Interleukin-17이 배양된 류마티스관절염 활막세포에서 vascular endothelial growth factor 생성에 미치는 영향

곽임수 ( Ihm Soo Kwak ),남태수 ( Tae Soo Nam ),나하연 ( Ha Yeon Rha ),서정탁 ( Jeung Tak Suh ),김유선 ( Yoo Sun Kim ),김성일 ( Sung Il Kim ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.3

Objective: To investigate the the effects of interleukin-17 (IL-17) on the production of vascular endothelial growth factor (VEGF) from cultured rheumatoid arthritis synoviocytes. Methods: Fibroblast-like synovial cells(FLS) were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-β(TGF-β), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β). VEGF levels were determined in the culture supernatants by sandwitch ELISA. Results: Stimulation of FLS by serial concentration of IL-17, TGF-β, TNF-α, IL-1β increased the production of VEGF by 2.1-2.7, 2.2-3.0, 2.0-2.9, 2.3-3.1 fold over the constitutive levels of unstimulated FLS. Stimulation of FLS by IL-17 with TGF-β or TNF-α or IL-1β also increased the production of VEGF according to culture periods by 1.6-1.8, 1.1-1.9, 1.5-1.7 fold over the levels stimulated with TGF-β or TNF-α or IL-1β, respectively. This results indicated that IL-17 increased the effect of TGF-β, TNF-α, IL-1β on FLS, leading synergistic enhancement of VEGF production. Conclusion: IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.

흰쥐의 좌골신경 절단 후 요추척수에서 소교세포의 증식과 소멸에 따른 Cytokine의 발현

김상표,서성일,조영록,조승제,김승필,박종욱,곽정식,박종욱 대한병리학회 1998 Journal of Pathology and Translational Medicine Vol.32 No.2

桑黃 물추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 Nitric Oxide production에 미치는 영향

김상찬(Sang Chan Kim),정연숙(Youn Suk Jung),이재령(Jae Ryung Lee),김영우(Young Woo Kim),변부형(Boo Hyeong Byun),권택규(Teag Kyu Kwon),서성일(Seong Il Suh),변성희(Sung Hui Byun),권영규(Young Kyu Kwon) 한의병리학회 2004 동의생리병리학회지 Vol.18 No.3

고온 환경에서의 운동 시 서로 다른 수분 보충이 엘리트 운동선수들의 염증성 사이토카인 반응 및 면역 기능에 미치는 영향

백일영 ( Il Young Paik ),윤두상 ( Doo Sang Yun ),서상훈 ( Sang Hoon Suh ),노희태 ( Hee Tae Roh ) 한국스포츠정책과학원(구 한국스포츠개발원) 2011 체육과학연구 Vol.22 No.3

이 연구의 목적은 고온 환경에서의 운동 시 단순 수분 및 스포츠 음료를 통한 수분 보충이 엘리트 운동선수들의 호르몬 변화, 염증성 사이토카인 반응 및 면역 기능에 미치는 영향을 규명하는데 있다. 연구의 대상은 엘리트 운동선수 10명으로 하였으며, 4가지 실험 처치 조건에서 75%HRR 운동 강도로 60분간 트레드밀 달리기를 실시하였다: 1) 적정 환경에서 운동 처치 조건(TE), 2) 고온 환경에서 운동 처치 조건(HE), 3) 고온환경에서 운동 시 물 섭취 처치 조건(HW), 4) 고온 환경에서 운동 시 스포츠음료 섭취 처치 조건(HS). 기후조건은 인공기후실을 이용하여 적정 환경은 온도 18℃, 상대습도 50%로 설정하였으며, 고온 환경은 온도 32℃, 상대습도 50%로 설정하였다. 수분 보충은 물 또는 스포츠음료를 이용하여 고온 환경에서 운동 처치 조건(HE)의 운동 전, 후 체중 차와 동일한 양을 구강 투여하였다. 채혈은 안정 시, 운동 직후, 회복 60분 시점에서 실시하였다. 연구결과, 운동 직후 시점에서 epinephrine은 HE 처치가 다른 세 처치(TE, HW, HS)보다 유의하게 높았으며(p<.05), norepinephrine은 HE 처치가 TE 처치보다 유의하게 높았다(p<.05). IL-6는 운동 직후 시점에서 HE 처치가 TE 처치보다 유의하게 높았다(p<.05). TNF-α는 TE 처치를 제외한 세 처치(HE, HW, HS)에서 운동 직후 유의하게 증가하였다(p<.05). T-cell은 TE 처치를 제외한 세 처치(HE, HW, HS)에서 운동 직후 유의하게 증가하였으며(p<.05), HE 처치가 TE 처치보다 유의하게 높았다(p<.05). NK-cell은 운동 직후 시점에서 HE 처치가 TE 처치보다 유의하게 높았다(p<.05). Neutrophil은 HE 처치의 경우 운동 직후 시점에서 유의하게 증가하였다(p<.05). Lymphocyte는 TE와 HE 처치에서 운동 직후 유의하게 증가하였다(p<.05). Monocyte는 운동 직후 시점에서 HE 처치가 TE 처치보다 유의하게 높았다(p<.05). 이상의 결과를 종합하면, 고온 환경에서의 운동은 적정 환경에서의 운동 시보다 카테콜아민과 염증성 사이토카인의 분비를 증가시켜 면역기능에 부정적인 영향을 미칠 수 있는 것으로 나타났다. 반면에 고온 환경에서의 운동 시 수분 보충은 탈수와 열적 스트레스를 경감시켜 면역기능의 부정적 영향을 완화시킬 수 있음을 시사한다. The purpose of the current research was to investigate the effects of body fluid replacement by water or sport drinks on the hormonal, inflammatory cytokine and immune responses during exercise in high ambient temperature. The subjects of the current research were 10 elite athletes. The subjects were asked to perform treadmill running at 75%HRR for 60 minutes in four different conditions, controlled by the environmental chamber: 1) thermoneutral environment (TE), 2) high ambient temperature environment (HE), 3) high ambient temperature environment and body fluid replacement by water (HW), and 4) high ambient temperature environment and body fluid replacement by sport drink(HS). Ambient temperature and relative humidity were set at 18℃ and 50%, respectively, for the theromoneutral environment, and 32℃ and 50% for the high ambient temperature environment. The amount of fluid replaced during two different fluid replacing conditions (HW and HS) were equivalent to the difference in body weight between pre and post completion of the running in HE. The defined amounts of the fluids were administered orally with separations during the exercise trials. Blood samples were taken at rest, at immediately after exercise, and at 60-minute of recovery. For catecholamine responses, the level of epinephrine immediately after exercise appeared to be significantly higher in HE compared to the other treatment conditions (p<.05), while the level of norepinephrine appeared to be significantly higher in HE compared to TE (p<.05). For cytokine responses, the level of IL-6 immediately after exercise appeared to be significantly higher in HE compared to TE (p<.05) while the levels of TNF-α were shown to significantly increase at immediately after exercise in all treatment conditions except TE (p<.05). For immune responses, the levels of T-cell were shown to significantly increase at immediately after exercise in all treatment conditions except TE (p<.05), and the level appeared to be significantly higher in HE compared to TE (p<.05); the level of NK-cell appeared to be higher at immediately after exercise in HE compare to TE (p<.05); the level of neutrophil was shown to significantly increase at immediately after exercise in HE (p<.05); the levels of lymphocyte were shown to significantly increase in TE and HE at immediately after exercise (p<.05); and the level of monocyte appeared to be significantly higher in HE compared to TE at immediately after exercise (p<.05). On the base of the results of the current study, it is suggested that exercise in high ambient temperature can induce increase responses of catecholamines and cytokines which may negatively influence on immune functions. Body fluid replacements during exercise in high ambient temperature, however, can attenuate the negative consequences of thermal stress on immune functions by preventing dehydration and reducing thermal stress.

Inhibitory Effect of Farfarae Flos Water Extract on COX-2, iNOS Expression and Nitric Oxide Production in lipopolysaccharide - activated RAW 264.7 cells

Yoon Tae Gyoung,Byun Boo Hyeong,Kwon Teag Kyu,Suh Seong Il,Byun Sung Hui,Kwon Young Kyu,Kim Sang Chan The Physiological Society of Korean Medicine and T 2004 동의생리병리학회지 Vol.18 No.3

Farfrae Flos has been clinically used for the treatment of asthma in traditional oriental medicine. There is lack of studies regarding the effects of Farfrae Flos on the immunological activities. The present study was conducted to evaluate the effect of Farfrae Flos on the regulatory mechanism of cytokines and nitric oxide (NO) for the immunological activities in Raw 264.7 cells. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, Farfrae Flos water extract inhibited nitric oxide production in a dose-dependent manner and abrogated inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2). Farfrae Flos water extract did not affect on cell viability. To investigate the mechanism by which Farfrae Flos water extract inhibits iNOS and COX-2 gene expression, we examined the on the phospholylation of inhibitor κBα and production of TNF-α, IL-1β and IL-6. Results provided evidence that Farfrae Flos inhibited the production of interleukin-1β (IL-1β) and the activation of phospholylation of inhibitor κBα in Raw 264.7 cells activated with LPS. These findings suggest that Farfrae Flos can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.

금은화(金銀花) 및 금은화전초(金銀花全草)가 Raw 264.7 cell에서 LPS로 유도된 NO의 생성, iNOS, COX-2 및 cytokine에 미치는 영향

이동언,이재령,김영우,권영규,변성희,신상우,서성일,권택규,변준석,김상찬,Lee, Dong-Eun,Lee, Jae-Ryung,Kim, Young-Woo,Kwon, Young-Kyu,Byun, Sung-Hui,Shin, Sang-Woo,Suh, Seong-Il,Kwon, Taeg-Kyu,Byun, Joon-Seok,Kim, Sang-Chan 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.2

Lonicerae Flos has antibacterial effects against Staphylococcus aureus, streptococci, pneumococci, Bacillus dysenterii, Salmonella typhi, and paratyphoid. It is an antiviral agent. The herb has a cytoprotective effect against $CCl_{4}-induced$ hepatic injury. It has antilipemic action, interfering with lipid absorption from the gut. Nowadays this herb is used mainly in the treatment of upper respiratory infections, such as tonsillitis and acute laryngitis. It is also used in the treatment of skin suppurations, such as carbuncles, and to treat viral conjunctivitis, influenza, pneumonia, and mastitis. Lonicerae Flos is dried flower buds of Lonicera japonica, L. hypoglauca, L. confusa, or L. dasystyla. But, for the most part, we use whole plant of Lonicera japonica, as a flower bud of it. And, little is known of the original copy of effects of whole plant, except for the 'Bon-Cho-Gang-Mok', which is written the effects of flower of Lonicera japonica are equal to effects of leaves and branch of it. The present study was conducted to evaluate the effect of flower and whole plant of Lonicera japonica on the regulatory mechanism of cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) for the immunological activities in Raw 264.7 cells. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, flower and whole plant of Lonicera japonica water extracts inhibited nitric oxide production in a dose-dependent manner and abrogated iNOS and COX-2. Flower and whole plant of Lonicera japonica water extract did not affect on cell viability. To investigate the mechanism by which flower and whole plant of Lonicera japonica water extract inhibits iNOS and COX-2 gene expression, we examined the on phosphorylation of inhibitor ${\kappa}B{\alpha}$ and assessed production of $TNF-{\alpha}$, $interleukin-1{\beta}$ $(IL-1{\beta})$ and interleukin-6 (IL-6). Results provided evidence that flower and whole plant of Lonicera japonica inhibited the production of $IL-1{\beta}$, IL-6 and activated the phosphorylation of inhibitor ${\kappa}B{\alpha}$ in Raw 264.7 cells activated with LPS. These findings suggest that flower and whole plant of Lonicera japonica can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections, respectively.

고교 축구선수의 경기 후 회복방법의 차이가 혈중 염증, 근 손상 및 피로 지표에 미치는 영향

백승훈(Seung Hoon Paik),백일영(Il Young Paik),서상훈(Sang Hoon Suh),조수연(Su Youn Cho),노희태(Hee Tae Roh) 한국사회체육학회 2014 한국사회체육학회지 Vol.0 No.56

The purpose of the current study was to investigate the effects of different recovery treatments on serum inflammation, muscle damage and fatigue variables after a game of soccer players in high school. The subjects of current study were 10 soccer players in high school without any medical complications. All subjects received both recovery treatments (contrast bath and massage) for once and a control application; therefore, all underwent 3 experimental conditions. Blood sampling taken at rest, at immediately after the match, and at 60 minutes recovery. From the three blood samples, the parameters of inflammation and fatigue were analyzed. Additional blood sampling was taken at 24 hours recovery for the analysis of the long term effect of the applications on the parameters of muscle damage. The concentration of interleukin-6(IL-6), creatine kinase(CK), lactate dehydrogenase (LDH), and lactate were shown to significantly increase at immediately after the match in all experimental conditions(p<.05). For inflammation responses the concentration of IL-6 60min recovery appeared to be significantly lower in contrast bath and massage treatments compared to control treatment(p<.05). Also, for muscle damages the concentration of CK 24h recovery appeared to be significantly lower in massage treatment compared to contrast bath and control treatments(p<.05). On the base of the results of the current study, it is suggested that soccer match can induce increased concentrations of IL-6, CK, LDH and lactate which may influence on inflammation, muscle damage and fatigue. However, contrast bath and massage were beneficial for recovery from muscle damage.

The association of disease activity, pro-inflammatory cytokines, and neurotrophic factors with depression in patients with rheumatoid arthritis

Cheon, Yun-Hong,Lee, Seung-Geun,Kim, Mingyo,Kim, Hyun-Ok,Sun Suh, Young,Park, Ki-Soo,Kim, Rock Bum,Yang, Hyun-Su,Kim, Ji-Min,Son, Chang-Nam,Kyoung Park, Eun,Kim, Sang-Hyon,Lee, Sang-Il Elsevier 2018 Brain, behavior, and immunity Vol.73 No.-

<P><B>Abstract</B></P> <P>Inflammation and trophic factors (brain-derived neurotrophic factor [BDNF], vascular endothelial growth factor, glial cell line-derived neurotrophic factor, and insulin-like growth factor-1) are associated with depression in the general population. Rheumatoid arthritis (RA) is a chronic representative inflammatory autoimmune disease; however, the association of disease activity, pro-inflammatory cytokines, and neurotrophic factors with depression has not been sufficiently investigated. Therefore, we determined the prevalence of depression and risk factors for depression and deterioration of depressive symptoms in RA patients. In addition, we analyzed the association between disease activity, pro-inflammatory cytokines, trophic factors, and depression in RA (N = 474). Demographic and laboratory data were examined, and routine assessment of patient index data 3 (RAPID 3) and disease activity score 28-joint count C-reactive protein (DAS 28-CRP) was performed to assess disease activity of RA. Depression was measured using the Korean version of the Beck Depression Inventory-second edition (K-BDI II). A K-BDI score ≥18 was considered the cut-off for depression in accordance with a previous validation study. The serum level of pro-inflammatory cytokines and neurotrophic factors was assessed by enzyme-linked immune sorbent assay. The prevalence of depression was 32.4% in patients with RA. The severity of disease activity of RA (RAPID 3 score [OR 2.34; 95% confidence interval, CI 1.22–4.51], DAS 28-CRP [≥3.2] [OR 1.60, 95% CI 1.01–2.53]) and severity of fatigue (OR 1.26 95% CI 1.15–1.38) were associated with depression and deterioration of depressive symptoms in the multivariate analysis. Among the components of RAPID 3 and DAS 28-CRP, patient assessment for global health and abilities for daily performance were more related to depression. The level of pro-inflammatory cytokines (IL-1β, IL-6, TNF-alpha) was not related to depression. The level of BDNF was significantly lower in RA patients with depression and was negatively correlated with K-BDI II score. Depression was related with the level of fatigue, low expression of BDNF, and high RA disease activity, which was associated with impaired ability to perform activities of daily life. Strict control of fatigue and disease activity to improve one’s capacity to perform daily life activities would be important to regulate depression. The level of BDNF might be one of the possible biomarkers to predict or monitor depression in patients with RA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Prevalence of depression in patients with rheumatoid arthritis (RA) was analyzed. </LI> <LI> Risk factors for depression and deterioration of depressive symptoms and were analyzed. </LI> <LI> Depression was related with disease activity in RA. </LI> <LI> Depression in RA was associated with impaired ability to perform activities of daily life. </LI> <LI> Among neurotrophic factors, the level of BDNF was related with depression in RA patients. </LI> <LI> The level of pro-inflammatory cytokines were not related with depression in RA patients. </LI> </UL> </P>

規則的 反射條件으로 分類된 空間群

徐日煥,金光柱,秋錦洪,李珍昊,林星秀,柳保盈,金文執 충남대학교 자연과학연구소 1992 忠南科學硏究誌 Vol.19 No.1

各 結晶格子는 230個 空間群 中 하나로 記述되는 對稱性을 가지고 있으며 그 結晶의 構造를 밝히기 爲하여는 그 結晶이 屬한 空間群을 반드시 알아야 한다. 이 論文에서는 230個 空間群을, 그 結晶이 가지고 있는 反射條件에 依하여 明白히 決定되는 空間群과 空間群間의 共通的인 反射條件 때문에 結晶의 構造가 밝혀진 後에야 밝혀지는 空間群으로 分類하여 놓았다. Every crystal lattice has a symmetry which is described by one of the 230 space groups and the space group of a crystal must be known in order to elucidate its structure. In this paper the 230 space groups are classified into two categories, i.e., the space groups that are explicitly determined by the reflection conditions the crystals have and the others that are determined only after the elucidation of the crystal structures because of the common systematic reflection conditions between the space groups.

7個 結晶系의 唯一 X-線 回折强度 資料

徐日煥,金光柱,秋錦洪,李珍昊,林星秀,柳保盈,金文執 충남대학교 자연과학연구소 1992 忠南科學硏究誌 Vol.19 No.1

한 結晶의 構造는 唯一單位의 X-線 回折强度資料로부터 決定될 수 있다. Miller指數(hkl)의 函數인 回折强度Ⅰ(hkl)는 異常 散亂(anomalous scattering)이 無視될때 對稱中心點群 卽 Laue群의 對稱性을 나타낸다. 이 論文에서는 모든 結晶系에 對한 回折强度의 唯一單位를 Laue群들의 對稱性으로부터 誘導하여 놓았다. Crystal structures can be determined from the unique data of X-ray intensity. As the X-ray intensity Ⅰ(hkl), which is a function of diffraction plane (hkl), has a center of symmetry assuming anomalous scattering is negligible Ⅰ(hkl) shows the symmetry of a centrosymmetric point group, i.e., a Laue group. In this paper the unique data of the intensity for all crystal systems have been derived from the symmetries of Laue groups.