개의 상후두신경 뇌간유발반응에 대한 실험적 연구

김광문,김영호,김광문,김영호 대한이비인후과학회 1998 대한이비인후과학회지 두경부외과학 Vol.41 No.4

염색체 위치 특이적 삽입과 안정적인 유전자 발현을 유도하는 플라스미드 백터의 제작

문영준,강윤성,최지혜,손진숙,민나영,이광호 中央大學校 基礎科學硏究所 2002 基礎科學硏究所 論文集 Vol.16 No.-

Insertion of reporter constructs into the mammalian genome leads to variable gene expression due to position effects at the site of integration. This random integration has limited the gene therapy of human genetic disorders by its undesirable effects. We report here the newly constructed plasmid vector(pIRES-neo-YJ) based on the concepts of homologous recombination and position-independent promoter enhancing of beta-globin matrix attachment region(Glb-MAR). chromosome 7 centromere-specific alpha satellite(alphoid) DNA sequence was cloned into pIRES-neo-YJ for homologous recombination of the cloned gene with the centromeric region of chromosome 7, which is genetically silent. Beta Glb-MAR sequence that allows high levels of transcription independent of the chromosomal site of integration was also inserted into pIRES-neo-YJ to ensure the stable and higher expression of the cloned genes. We expect that pIRES-neo-YJ would provide a valuable tool to eliminate random integration of cloned genes into the undesirable chromosomal region and their short-lived expression which often encounters during the construction of transgenic animals and human gene therapy.

연안산 패류를 이용한 조미소재의 가공 및 정미성분

문정호,강수태,김종태,오광수 경상대학교 해양산업연구소 2002 해양산업연구소보 Vol.15 No.-

고부가가치를 창출할 수 있는 새로운 식품가공용 풍미조미소계의 개발과 품질의 개선, 그리고 연안에서 생산되는 수산자원의 유효이용이라는 관점에서, 고부가 활용도가 거의 없는 바지락을 원료로 하여 고부가가치의 풍미계 조미소재 및 수프 소재로서 활용될 수 있는 유용 조미소재의 최적 가공조건을 구명하였고, 이의 정미성분을 분석하였다. 바지락 2단 효소분해 조미소재는 최적 가공공정은 다음과 같다. 시료 바지락을 chopper로써 세절한 다음 약 3배량의 물을 가하고, 98℃에서 5분간 자숙하여 자가소화효소를 불활성화시킨 후, 시료액의 pH를 8.0으로 조정하고 여기에 활성이 비교적 약한 내알칼리성 단백분해효소 가하여 교반하면서 55℃에서 4시간 동안 가수분해시킨다. 이어 자숙 처리하여 효소를 불활성화시킨 후 다시 pH를 6.0으로 조정하고, 여기에 exopeptidase 형의 중성 단백분해효소를 가한 다음 45℃에서 교반하면서 4시간 동안 가수분해시키고, 효소를 불활성화시킨 후 원심분리하여 상등액을 취함으로서 효소분해 조미소재를 얻을 수 있었다. 열수추출법, 자가소화법 및 2단효소분해법으로 바지락 조미소재를 조제하고, 각 조미소재의 특성을 서로 비교 검토한 결과, 바지락 열수 및 가압추출 조미소재에서는 대체로 감칠맛이 났으나 맛의 강도가 약하였고 패류취가 감지되었다. 바지락 2단 효소분해 조미소재는 감칠맛과 맛의 조화력이 월등히 강하였고, 특히 투명도 상승 등 조미소재의 물리적, 관능적 품질이 현저히 개선되었다. 바지락 열수 및 가압추출 조미소재, 2단효소분해 조미소재의 유리아미노산 총량은 각각 1,352.1 mg/100g, 1,174.1 mg/100g 및 2,122.4 mg/100g이었고, glutamic acid, glycine, alanine, tyrosine, phonylalanine, arginine 등이 주요 유리아미노산으로 추출방법에 따라 아미노산의 농도변화가 켰다. Betaine 함량은 116.O~845.0 mg/100g으로 열수추출이나 가압추출에 비해 효소분해 조미소재의 함량이 월등히 많았으며, 타 정미성분과도 함량을 비교해 볼 때 바지락 조미소재의 주된 정미성분일 것으로 추정되었다. 무기이온으로서 P, Na, K 및 Cl의 함량이 많았으며, 열수나 가압추출 조미소재에 비해 효소분해 조미소재 쪽이 함량이 훨씬 많았다. To develop natural flavoring substances, optimal two stage enzyme hydrolysis conditions and flavour compounds for short-neck clam enzyme hydrolysates were examined. The optimal enzyme hydrolysis conditions for two stage enzyme hydrolysate(TSEH) of short-neck clam were revealed in temperature at 55℃ for 4 hours digestion with alcalase at the 1st stage and 4 hours digestion at 45℃ with exopeptidase type neutrase at the 2nd stage. From the results in quality tests of hot-water extracts, steam extracts and 4 kinds of enzyme hydrolysates, TSEH processing method was superior to other methods on the aspects of yield, nitrogen contents, taste such as umami intensity and inhibition of off-flavor formation, and transparency of extract. And the flavor constituents and functionality of short-neck clam were also examined as affected by two stage enzyme hydrolysis. Total free amino acid contents in hot-water extract steam extract and TSEH of short-neck clam were 1,352.1 mg%, 1,174.1 mg% and 2,122.4 mg%, respectively. Major free amino acids in TSEH were glutamic acid, glycine, alanine, tyrosine, phenylalanine, arginine. As for nucleotides and other bases, betaine, TMAO and creatinine were principal components in TSEH. And the major inorganic ions in TSEH were Na, K, p and Cl

적외선 흡수 분광법 및 DPC를 이용한 치과용 수복재의 광중합 거동 분석

정찬문,김중곤,최준호,장두옥,김경남,김광만 대한치과기재학회 1998 대한치과재료학회지 Vol.25 No.1

Mechanical properties of compomers would be obtained mainly by photopolymerization by visible light irradiation. Photopolymerization behavior of two commercial compomers was investigated by FT-IR spectral analysis and differential photocalorimetry(DPC). The results obtained were as follows: 1. From FT-IR analysis, it was found that the compomer products were photocured mainly by radical polymerizations of multifunctional methacrylate monomers. Final conversion values of the polymerizations after exposure to visible light for 60 sec were 67% for Dyract and 59% for Compoglass. 2. Upon exposure the compomers exhibited autoacceleration and autodeceleration, which are usually observed in bulk polymerizations of multifunctional monomers. 3. Dyract showed higher rate of polymerization and conversion of double bond than Compoglass.

습식 TCE 분해반응에서 CoO_(x)/TiO₂ 촉매의 반응활성 및 표면화학적 구조

김문현,추광호 한국환경과학회 2005 한국환경과학회지 Vol.14 No.2

Catalytic wet oxidation of trichloroethylene (TCE) in water has been conducted using TiO₂-supported cobalt oxides at 36℃ with a weight hourly space velocity of 7,500 h^(-1). 5% CoO_(X)/TiO₂, prepared by using an incipient wetness technique, might be the most promising catalyst for the wet oxidation although it exhibited a transient behavior in time on-stream activity. Not only could the bare support be inactive for the wet decomposition reaction, but no TCE removal also occurred by the process of adsorption on TiO₂ surface. The catalytic activity was independent of all particle sizes used, thereby representing no mass transfer limitation in intraparticle diffusion. XPS spectra of both fresh and used Co surfaces gave different surface spectral features for each CoO_(X+). Co 2p_(3/2) binding energy for Co species in the fresh catalyst appeared at 781.3 eV, which is very similar to the chemical states of CoTiO_(X) such as CO₂TiO₄ and CoTiO₃. The used catalyst exhibited a 780.3-eV main peak with a satellite structure at 795.8 eV. Based on XPS spectra of reference Co compound, the TCE-exposed Co surfaces could be assigned to be in the form of mainly CO₃O₄. XRD patterns for 5% CoOx/TiO₂ catalyst indicated that the phase structure of Co species in the catalyst even before reaction is quite comparable to the diffraction lines of external Co3O4 standard. A model structure of CoO_(X) present predominantly on titania surfaces would be CoO₃O₄, encapsulated in thin-film CoTiO_(X) species consisting of CO₂TiO₄ and CoTiO₃, which may be active for the decomposition of TCE in a flow of water.

아연 용탕 내에서 불순물의 입자 크기 측정에 관한 연구

이광학,문광호,김영홍,최욱태 울산대학교 1995 공학연구논문집 Vol.26 No.2

LiMCA방법으로 아연 용탕내에 Pb를 첨가하여 불순물의 입자크기를 측정하였다. 아연 용탕내에 Pb의 첨가량이 증가할수록 불순물의 수는 증가하였으며 불순물의 입자 분포는 일정범위를 크게 벗어나지 않고 d=50㎛이하의 입자직경이 약90% 이상 존재하였다. 실험 후 시편을 분석한 결과, 시편내에 미량의 ZnO와 PbO가 존재함을 확인하였으며, 이는 아연용탕 내에서 제 2상의 거동을 예상하고 첨가한 Pb에 의해 형성된 PbO와 극소량의 ZnO등이 불순물로 거동한 것으로 사료된다. 본 실험은 약1분 정도의 짧은 시간으로 수행되었으며, 실험결과 사용된 장치의 감지 한계는 불순물의 입자직경이 d=29㎛이었다. The particle size of impurities was measured with addition to Pb in the molten Zinc by using LiMCA method. The number of impurities was increased with increasing Pb addition in the molten Zinc and particle size distribution of impurities was not deviated from the constant range therefore the particle size, d=50㎛ existed about 90% in this study. The experimental results of X-ray analysis, it was found that the small amount of PbO and ZnO which are formed with Pb addition to the molten Zinc, expecting the second particle role, behavior as impurities. This study was carried out about one minute and the detect limit of particle size of impurities using instrument was obtained d=29㎛.

급성 근골격질환에 있어서 피록시캄 근육주사제의 임상적 연구 : An Open, Non-Comparative Trial

윤승호,이광진,김학영,문형석,조성일 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

The authors have conducted a clinical study to evaluate the efficacy and toleration with newly developed piroxicam injectable (I. M.) to thirty-one cases of the patients of musculoskeletal disorders during five months from Oct. 1986 to Feb. 1987. Treatment for the patients was initiated with two amples of piroxicam 20mg I. M. daily for first two days and followed administration one ample of 20mg piroxicam daily for three to seven days. Results were as follows: 1. Out of 31 cases, male were 16 (50.5%), female 15(49.5%), and predominant age group was 20-29 years old 11(35.4%). 2. Major indications were sprained ankle and sprained PIP joint disorders 11(35.7%), 12(38.7 %), respectively. 3. Overall efficacy, more than good was 83.8% by patients and 96.8% by physician. 4. Overall tolerance, more than good was 90.3% by patients and 90.3% by physician (excluded four cases). 5. Side effects were seen in 4 patients such as I case of constipation, 2 cases of abdominal pain and 1 case of vomiting. 6. Conclusion: The piroxicam I. M. was very effective and well tolerated for the treatment of musculoskeletal disorders and side effects were few and generally mild.

반도체 전공정 제어용 온도제어기 설계 및 구현

한광록,문세호 호서대학교반도체제조장비국산화 연구센터 2000 반도체 장비기술 논문집 Vol.2000 No.-

반도체 양산공정에 적용되는 장비에 있어서 선공정용 장비의 정밀한 온도의 제어는 반도체의 품질과 전체 반도체 제조 공정의 품질을 결정하는 주된 용인으로 작용되므로, 전공정 양산 장비에 대응할 수 있고, 특히 OXIDATION, ANNEAL 및 DEFFUSION 공정에 적용할수 있도록 예측기능을 갖는 온도제어 모듈을 설계 및 구현한다.

전국 응급의학과 수련병원의 응급실 병력지에 대한 분석

임태호,임훈,이종호,강형구,장문준,조광현,장석준,김승호,정상원 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: This study was designed to analyze the current emergency department(ED) medical records of teaching hospitals in Korea. Methods: The five-item questionnaires were mailed to the EDs of 40 hospitals. Among them, 27 questionnaires and 35 ED medical records were returned for reply rates of 67.5% and 87.5%, respectively. Results: 1) The actual number of data elements in the ED medical records used by each hospital varies widely. It ranges from 1 to 15 data elements with an average of 7.5 data elements. 2) Thirteen data elements, signature of nurse, checklist style in review of systems, checklist style in physical examination, neurologic examination, figure of face, Glasgow coma scale, trauma scale, treatment plan, mode of transfer, condition on transfer, documents sent with patient, condition on discharge or discharge instruction, use of pediatric chart and vaccination history are used by less than 50% of the medical records examined. 3) There was no difference in the total number of data elements or in redesign and computerization of ED medical record based on the location of the hospital, the type of hospital administration, or the number of years since the start of EM residency program. 4) There was a statistically increased number of data elements in redesigned medical records. 5) In the survey, 89% of the residents replied that medical records needed to be redesigned. With respect to uniformity, 58% of the residents disagreed. A well-designed checklist chart rather than a descriptive chart was preferred by 89% of the residents. Conclusion: The currently used ED medical records have much room for improvement. The age of the ED had little impact on the quality of ED medical records. More attention and effort in this field are needed. In addition, The Korean Society of Emergency Medicine should provide guidelines for ED medical records.

정어리 냉동고기풀의 품질개선에 관한 연구

오광수,문수경,이응호,김복규 國立統營水産專門大學 附設 水産科學硏究所 1994 수산과학연구소보고 Vol.5 No.-

정어리 냉동고기풀의 최적 가공조건은 원료 정어리를 알칼리 염수수세한 후 정어리 육에 대해 분리 대두단백질:물:정제 정어리유(1:5:2.6)로 만든 유화커드를 20%, 솔비톨 4%, 설탕 4%, 중합인산염 0.2% 및 항산화제로서 에리소르빈산나트륨을 0.1% 첨가하여 고기갈이한 후 -35℃에서 급속동결시켜 carbon box로 포장하여 동결저장한 제품이 가장 좋았다. 본 제품의 수분 함량은 73.3%. 조단백질 15.0%, 조지방은 6.9%였으며, 생균수는 1.6~l.8X10^4/g으로 동결저장 중 변화는 거의 없었다. 제품의 주요 구성지방산은 16:0, 16:1, 18:1, 20:5 및 22:6 등으로 고도 불포화지방산이 47.7% 함유되어 있었다. 동결저장 중 120일 동안 제품의 물성, 지질산화 및 지방산의 변화 등을 측정한 결과, 유화커드와 에리소르빈산나트륨을 첨가함으로서 동결저장 중 단백질 변성, 지질의 산화 및 변색을 효율적으로 억제 시킬 수 있었으며, 품질이 안정하게 유지되었다. 한편, 본 시제품과 명태 냉동고기풀을 혼합하여 가공한 어묵의 품질을 측정한 결과, 어묵의 품질에 큰 저하됨이 없이 명태 고기풀을 본 정어리 고기풀로 40%까지 대체할 수 있었으며 어육의 풍미를 갖는 연제품을 가공할 수 있다는 결론을 얻었다. The processing conditions and quality of sardine surimi were examined: Raw sardine meat was separated, washed in 0.2% NaHC0_3 and 0.15% NaCl solution, and then dewatered by centrifuge. The dewatered sardine meat was chopped, mixed with 20% emulsion curd (soybean protein : water : refined sardine oil= 1 : 5. 2.6), 4% sorbitol, 4% sucrose, 0.2% polyphosphate and 0.1% sodium erythorbate by stone mortar. The mixed sardine meat was frozen with contact freezer, packed in carton box and then stored at -25±2℃. The moisture, crude protein and lipid contents of the sardine surimi product was 73.3%, 15.0% and 6.9%, respectively. Fatty acid composition of product consisted of 28.8% of saturates, 24.3% of monoenes and 47.7% of polyenes and the major fatty acids were 16:0, 20 5, 18:1, 22:6 and 16:1. The results of changes in POV, TBA value, fatty acids, texture and sensory score of products during frozen storage showed that lipid oxidation and freeze denaturation of product could be retarded, and flavor enhanced by addition 20% emulsion curd and 0.1% sodium erythorbate. In an attempt to apply sardine surimi in producing surimi-based product, it was concluded that Pollack surimi could be substituted with sardine surimi up to 40% without showing any significant changes in texture and taste of surimi-based product.