In vivo study for the hemostatic efficacy and foreign body reaction of a new powder-type polysaccharide hemostatic agent

Yoonhyeong Byun,Eun Jin Kim,Areum Lee,Young-Ah Suh,Hee Ju Sohn,Jung Min Lee,Jae Seung Kang,Yoo Jin Choi,Youngmin Han,Hongbeom Kim,Wooil Kwon,Jin-Young Jang 대한외과학회 2022 Annals of Surgical Treatment and Research(ASRT) Vol.102 No.2

Purpose: Various hemostatic agents have been introduced in therapy as postoperative bleeding is a poor prognostic factor for postoperative outcomes. These products can be divided into those that directly promote the hemostatic cascade and those that physically form a barrier by absorbing blood. The latter, powder-type hemostatic agents have the advantages of being inexpensive and more absorbable with less foreign body reactions (FBRs) and are applicable to a relatively wide area. This study was conducted to verify the safety and efficacy of a newly invented polysaccharide product (OOZFIX, Theracion Biomedical), which improves blood absorption and hemostatic effects. Methods: Two separate animal experiments were performed. The first evaluated FBRs histologically at 3 days, 2 weeks, and 4 weeks, after implantation of OOZFIX in rats, and the second compared hemostatic performance of OOZFIX and Arista AH (Bard) in the porcine liver punch biopsy model. Results: We found minimal FBRs in the 3-day group and no reactions in both the 2-week and 4-week groups after implantation of hemostatic agents. The time to hemostasis of OOZFIX was not significantly different from that of Arista AH (median [interquartile range]: 9 [6–10] minutes vs. 8 [6–10] minutes, respectively; P = 0.522). When comparing the serial bleeding grade tendency, there was no statistical difference between OOZFIX and Arista AH (P = 0.656). Conclusion: OOZFIX caused a minimal FBR that disappeared within 2 weeks in vivo, and its hemostatic performance was comparable with that of an existing agent, Arista AH. Further clinical studies are required in the future.

침 시술로 발생한 Non-O1, Non-O139 Vibrio cholerae에 의한 패혈증

임태섭,지아영,이중희,장수연,김인수,김영주,김범경,김승업,박준용,안상훈,한광협,김도영 Ewha Womans University School of Medicine 2013 EMJ (Ewha medical journal) Vol.36 No.S

Vibrio cholerae is mainly known to cause gastrointestinal infection after seawater exposure or raw seafood intake. It is rarely reported to cause cellulitis or sepsis, but threre has been no known case after acupuncture. Herein, We report a 56-year-old cirrhotic patient of non-O1, non-O139 Vibrio cholerae septicemia caused by cellulitis of both lower extremities after acupuncture.

에어로빅 운동이 비만 여중생의 복부비만과 심폐기능 및 인슐린에 미치는 영향

김기봉,신주화,이정아 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

The purpose of this study was to analyze the effects of aerobic exercise on abdominal obesity, cardiopulmonary function and serum insulin in obese middle school girls participated in aerobic exercise for 20 weeks. The conclusions are as follows ; 1. Total body fat, percent body fat, body mass index, waist circumference and waist-hip ratio were significantly decreased, but lean body mass was significantly increased in the changes of body composition factors. 2. Systolic blood pressure, diastolic blood pressure were significantly decreased, but maximal oxygen consumption was significantly increased in the changes of cardiopulmonary function factors. 3. Total cholesterol, triglyceride, low-density lipoprotein cholesterol and insulin were significantly decreased, but high-density lipoprotein cholesterol was significantly increased in the changes of serum lipid and insulin factors. In conclusions, aerobic exercise may be very effective to decrease the obesity and geriatric diseases.

Family Origin Names and Haplotypes of Y - DNA in Korean Population

Kim, Jong Soon,Kim, Yung Jin,Ahn, Gwang Sook,Lee, Ju Won,You, Sun Ah 한국유전학회 1996 Genes & Genomics Vol.18 No.4

The use of restriction fragment length polymorphisms (RFLPs) has been proposed for the construction of a human genetic linkage map and also become a powerful tool in human population genetics. Human Y-chromosome which is haploid and paternally inherited is valuable for investigating male-mediated gene flow and for complement maternally based studies of mtDNA. We have studied 166 samples from 4 kinds of family origin names such as Kyungju Kim, Kimhae Kim, Chunju Lee and Milyang Park based on the 49aTaq 1 polymorphisms, and a total of 25 haplotypes were observed. The B, F, and I bands is observed in all haplotypes and haplotype showing A3, B, D2, D3, F, and I bands is common haplotype except for Chunju Lee. The result obtained are summarized as follows.

EGFR 돌연변이와 ROS1 전위를 동시에 가진 폐선암 환자의 Erlotinib 치료 1예

김민환,박예현,박혜정,지아영,송창호,진무년,김영주,김선욱,이중희,김인수,김혜련,김주항,조병철 Ewha Womans University School of Medicine 2014 EMJ (Ewha medical journal) Vol.37 No.1

The rearrangement of c-ros oncogene 1 (ROS1) has been recently identified as an important molecular target in non small cell lung cancer (NSCLC). ROS1 rearrangement and epidermal growth factor receptor (EGFR) mutation were mutually exclusive each other in previous studies, and the clinical implication of co-existence of the two genetic alterations has not been determined. We report a case of 46-year-old female never-smoker NSCLC patient whose tumor harbored ROS1 rearrangement and EGFR mutation concomitantly. She had undergone curative surgery for stage IIIA NSCLC, and the recurrence in left pleura and brain occurred at 2 years after the surgery. She received several lines of chemotherapy including docetaxel plus carboplatin, erlotinib, pemetrexed, and gemcitabine. Erlotinib therapy showed a favorable treatment response with progression-free survival of 9.5 months and partial response of tumor on radiologic evaluations. This case represents a successful erlotinib treatment in a NSCLC patient with concurrent ROS1 rearrangement and EGFR mutation.

Blood-letting therapy for the common cold : A protocol for a systematic review of controlled trials

Lee, Ju Ah,Hong, Minna,Lee, Myeong Soo,Yoon, Seong Hoon,Choi, Jun-Yong Williams & Wilkins Co 2017 Medicine Vol.96 No.51

<P><B>Abstract</B></P><P><B>Background:</B></P><P>Many people experience the common cold, but there is currently no special treatment. For this reason, complementary and alternative medicine (CAM) therapies are used to improve the symptoms of the common cold. Blood-letting therapy (BL) is a CAM therapy that has been used for over 2000 years to treat various diseases. However, few studies have provided evidence for the efficacy and safety of BL for the common cold. This study aims to assess the effectiveness and safety of BL for the common cold.</P><P><B>Methods and analysis:</B></P><P>A total of 11 databases will be searched for studies conducted through June 2017. We will include randomized controlled trials assessing BL for the common cold. All randomized controlled trials on BL or related interventions will be included. Risk of bias will be assessed using the Cochrane Risk of Bias Assessment Tool, while confidence in the accumulated evidence will be evaluated using the Grading of Recommendations Assessment, Development and Evaluation instrument.</P><P><B>Ethics and dissemination:</B></P><P>This systematic review will be published in a peer-reviewed journal and will also be disseminated electronically and in print. The review will be updated to inform and guide healthcare practices.</P>

Hypoglycemia at Admission in Patients With Acute Myocardial Infarction Predicts a Higher 30-Day Mortality in Patients With Poorly Controlled Type 2 Diabetes Than in Well-Controlled Patients

Lee, Sang Ah,Cho, Suk Ju,Jeong, Myung Ho,Kim, Young Jo,Kim, Chong Jin,Cho, Myeong Chan,Kim, Hyo-Soo,Ahn, Youngkeun,Koh, Gwanpyo,Lee, Jeong mi,Oh, Seok Kyu,Yun, Kyeong Ho,Kim, Ha Young,Cho, Chung Gu,Le American Diabetes Association 2014 Diabetes Care Vol.37 No.8

<P><B>OBJECTIVE</B></P><P>We aimed to evaluate the association between hypoglycemia at admission and 30-day mortality in patients with acute myocardial infarction (AMI) and to determine whether these associations differed according to diabetes-control status in AMI patients with diabetes.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>We analyzed the prognostic significance of hypoglycemia and hyperglycemia in 34,943 AMI patients with or without type 2 diabetes from two AMI registries: the Korea Acute Myocardial Infarction Registry (KAMIR) and the Korea Working Group on Myocardial Infarction (KorMI).</P><P><B>RESULTS</B></P><P>The patients were divided into five groups according to serum-glucose levels at admission: <3.9 mmol/L (<70 mg/dL); 3.9–7.72 mmol/L (70–139 mg/dL); 7.78–11.06 mmol/L (140–199 mg/dL); 11.11–14.39 mmol/L (200–259 mg/dL); and ≥14.44 mmol/L (≥260 mg/dL). The 30-day mortality rates in the lowest and highest glucose groups were higher than those in other groups; the lowest glucose group had the highest mortality for patients with type 2 diabetes, after adjusting for multiple factors. We also extracted and compared four subgroups from the patients with type 2 diabetes, based on hemoglobin A1c and serum-glucose levels at admission: group A, <6.5% (48 mmol/mol) and <3.9 mmol/L; group B, <6.5% (48 mmol/mol) and ≥11.11 mmol/L; group C, ≥8% (64 mmol/mol) and <3.9 mmol/L; and group D, ≥8% (64 mmol/mol) and ≥11.11 mmol/L. Group C had the highest 30-day mortality rate among the groups.</P><P><B>CONCLUSIONS</B></P><P>These data suggest that hypoglycemia at admission affects clinical outcomes differently in AMI patients with type 2 diabetes depending on the diabetes-control status.</P>

Cognition and memory function of Taraxacum coreanum in an in vivo amyloid-β-induced mouse model of Alzheimer’s disease

Lee Young, Ah,Yamabe, Noriko,Kang Sung, Ki,Kim Hyun, Young,Lee, Sanghyun,Cho Eun, Ju National Library of Serbia 2014 Archives of biological sciences Vol.66 No.4

Association of vitamin D concentrations with adiposity indices among preadolescent children in Korea

Lee, Hye Ah,Kim, Young Ju,Lee, Hwayoung,Gwak, Hye Sun,Park, Eun Ae,Cho, Su Jin,Oh, Se Young,Ha, Eun Hee,Kim, Hae Soon,Park, Hyesook Walter de Gruyter GmbH 2013 Journal of pediatric endocrinology & metabolism Vol.26 No.9

<P>Background and Objective: We aimed to investigate the association between serum 25-hydroxyvitamin D (25(OH)D) and anthropometric indicators of adiposity among Korean preadolescent children aged 7?9 years. Subjects and Methods: Children aged 7 to 9 from the Ewha Birth & Growth Cohort were followed up from July to August 2011. Serum 25(OH)D concentration was measured using radio immunoassay. We assessed the magnitude of cross-sectional association with vitamin D concentrations and indicators of adiposity [body mass index (BMI), waist circumference (WC), mid-arm circumference (MAC), body fat mass (BFM), percent body fat (PBF), and triceps skinfold thickness (TSF)] using multiple linear and logistic regression analyses adjusted for sex, age, birth order, maternal education, and fruit/fruit juice intake. Results: Thirty-two (16%) out of a total of 205 children showed an optimal level of serum 25(OH)D, but most children did not reach a sufficient level (<30 ng/mL) even in summer. The level of 25(OH)D was inversely associated with BMI (β=?0.10, p<0.01), WC (β=?0.28, p<0.01), and BFM (β=?0.12, p=0.02) after adjusting for confounding factors and showed marginal boundary with PBF (β=?0.20, p=0.06) and TSF (β=?0.11, p=0.08). Regarding the risk of overweight, a 13% protective effect per 1 ng/mL increase of 25(OH)D was shown even after adjusting for relevant confounding factors (adjusted odds ratio=0.87, 95% confidence interval 0.78?0.98). Conclusions: We found that the 25(OH)D concentrations were inversely associated with adiposity indices in preadolescent children. This study suggests that adequate vitamin D intake in growing children is crucial to maintain an optimal vitamin D level to prevent obesity and obesity-related health problems later in life.</P>

Knockdown of vps54 aggravates tamoxifen-induced cytotoxicity in fission yeast

Lee, Sol,Nam, Miyoung,Lee, Ah-Reum,Baek, Seung-Tae,Kim, Min Jung,Kim, Ju Seong,Kong, Andrew Hyunsoo,Lee, Minho,Lee, Sook-Jeong,Kim, Seon-Young,Kim, Dong-Uk,Hoe, Kwang-Lae Korea Genome Organization 2021 Genomics & informatics Vol.19 No.4

Tamoxifen (TAM) is an anticancer drug used to treat estrogen receptor (ER)-positive breast cancer. However, its ER-independent cytotoxic and antifungal activities have prompted debates on its mechanism of action. To achieve a better understanding of the ER-independent antifungal action mechanisms of TAM, we systematically identified TAM-sensitive genes through microarray screening of the heterozygous gene deletion library in fission yeast (Schizosaccharomyces pombe). Secondary confirmation was followed by a spotting assay, finally yielding 13 TAM-sensitive genes under the drug-induced haploinsufficient condition. For these 13 TAM-sensitive genes, we conducted a comparative analysis of their Gene Ontology (GO) 'biological process' terms identified from other genome-wide screenings of the budding yeast deletion library and the MCF7 breast cancer cell line. Several TAM-sensitive genes overlapped between the yeast strains and MCF7 in GO terms including 'cell cycle' (cdc2, rik1, pas1, and leo1), 'signaling' (sck2, oga1, and cki3), and 'vesicle-mediated transport' (SPCC126.08c, vps54, sec72, and tvp15), suggesting their roles in the ER-independent cytotoxic effects of TAM. We recently reported that the cki3 gene with the 'signaling' GO term was related to the ER-independent antifungal action mechanisms of TAM in yeast. In this study, we report that haploinsufficiency of the essential vps54 gene, which encodes the GARP complex subunit, significantly aggravated TAM sensitivity and led to an enlarged vesicle structure in comparison with the SP286 control strain. These results strongly suggest that the vesicle-mediated transport process might be another action mechanism of the ER-independent antifungal or cytotoxic effects of TAM.