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고장식염수가 토끼 빈창자 점막 산호성 과립세포의 미세구조에 미치는 영향
문정석,윤상필,정윤영,허영,장인엽,김종중 朝鮮大學校 附設 醫學硏究所 1998 The Medical Journal of Chosun University Vol.23 No.1
This experiment was performed to study the influence of hypertonic saline on the production of the secretory granules by the cellular organelles of the jejunal Paneth cells of the rabbit fed on the 3 % hypertonic saline. The jejunal mucosae of the experimental rabbits were removed partially in the upper one third of the jejunum and fixed in 2.0% glutaraldehyde for 2 hours, followed by 1% osmium tetroxide fixative for 2 hours. Then they were embedded in epon mixture, cut with ultramicrotome and done uranyl acetate-lead citrate double stain, and observed with JEM100 CX Ⅱ electron microscope. Observed results were as follows: 1. The number of secretory granules of a higher electron density was gradually increased as a whole in the course of time after feeding on the hypertonic saline. 2. After feeding on the hypertonic saline, secretory granules of various sizes with a higher electron density mostly occupied the supranuclear region of Paneth cells, but a few secretory granules of various sizes with low electron density were observed. 3. After feeding on the hypertonic saline, the Goigi complex, the mitochondria and the rough surfaced endoplasmic reticula were gradually figured, especially the cisternae of the rough surfaced endoplasmic reticula displayed polyhedral shapes. 4. Considering the above findings, following the feeding on the hypertonic saline, the function of the jejunal Paneth cells was influenced and secretory granules of various sizes with a higher electron density were more abundant as compared with those of a lower electron density. From the above results, it was considered that the Golgi complex, the mitochondria and especially the rough surfaced endoplasmic reticula were activated to maintain homeostasis. As a result of that, the production and concentration of secretory granules were prominently observed.
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문창종,김승준,위명복,김희석,정종태,박전홍,지영흔,Tanumab, Naoyuki,Matsumoto, Yoh,신태균 濟州大學校 農科大學 動物科學硏究所 2000 動物科學論叢 Vol.15 No.1
The expression of pro-apoptotic molecules, p53 and Bax, in the spinal cord of rats with experimental autoimmune encephalomyelitis (EAE) was examined. Apoptosis was confirmed by the terminal deoxynucleotidyl transferasemediated dUTP nick end-labeling (TUNEL) method. TUSEL (+) apoptotic cells were mainly either ED1 (+) macrophages or T-cells in the parenchyma of E N . Western blot analysis showed that both p53 and Bax expression significantly ( p < 0.01) increased in the spinal cords of EAE rats at the peak stage, and thereafter declined. An immunohistochemical study showed that inflammatory cells (notably T cells) in the parenchyma express p53 and Bax, while brain cells, includng neurons and glia, were devoid of these nuclear staining of these molecules. The nuclear expression of p53 largely matches apoptotic cells in the parenchyma of EAE. These finchngs suggest that pro-apoptotic molecules, p53 and Bax, may play an important role in eliminating T cells in the parenchyma in EAE.
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척수를 손상시킨 후 꼬리정맥에 주입한 사람탯줄혈액세포가 뇌줄기에 미치는 영향
김종중,정윤영,박영란,문영민,현영식,정영욱,문정석 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1
Background and Objectives: Stem cells are a valuable resource for treatment of many disease, but limited access to stem cells in some organs such as brain restricts their utility. Many approaches have been attempted to restore the function following brain stem injury (BSI) and spinal cord injury (SCI). The use of the human umbilical cord blood cells (hUCB) - a rich source of nonembryonic or adult stem cells - has recently been reported to ameliorate the behavioral consequences of stroke. Mateiials and Methods: Forty rats were divided into 8 groups: (1) SCI l+hUCB (infused 1 day post injury); (2) SCI 2+hUCB (infused 2 days post injury); (3) SCI 3+hUCB (infused 3 days post injury); (4) SCI 4+hUCB (infused 4 days post injury); (5) SCI 5+hUCB (mfusedt 5 days post injury); (6) SCI 6+hUCB (infused 6 days post injury); (7) LO+hUCB (laminectomy+hUCB); and (8) LO (laminectomy only). SCI was produced by compressing the spinal cord for one minute with an aneurysm clip calibrated to a closing pressure of 50 g. We report here that immunhistotochemical identification of fluorescent hUCB positive cells in the brain stem after compressed spinal cord injury using mouse anti-human mitochondria monoclonal antibody (MAB1273). Results: All SCI+hUCB (1-8) groups contained fluorescent hUCB positive cells in the all area of the brain stem. But especially a large number of fluorescent hUCB positive cells were observed in the whole area of the brain stem of the experimental 5 (SCI 5+hUCB) and 6 (SCI 6+hUCB)groups. No hUCB positive cells were found in the brain stem of group with non-injured spinal cord of these animals and group with laminectomy only. Conclusion: These results suggest that hUCB are potentially useful as a vector for treating a variety of the central nervous system disorders, and we are sure that continuous of stem cell study will give an best opportunity to treat the uncurable disorders in the future.
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박상문,박준석,류명곤,김갑득,송화식,김종완 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4
Diagnostic peritoneal lavage is a accurate and safe method confirming the intraabdominal injury with head trauma, unstable multiple injuried patients. But the development of imaging technique and the trend in noninvasive diagnostic value is decreasing now. In the emergency room, the emergency physicians are required the rapidity and accuracy in intial assessment and the decisions for the operation or not. So emergency physicians have been investigating the diagnostic method for intraabdomial injury that is more rapid, safe and accurate. Many diagnostic tools are developed and now accepted and used widely, among them the diagnostic peritoneal lavage is still the best method for unsatble or head injuried patients. But the positive diagnostic criterias are variable by many authors and still remain imperfectly. So our study was reviewed by medical record of patients who were undertaken the diagnostic peritoneal lavage for the diagnosis intraabdominal injury and statistical analysis was done compared with many criteria of red blood cell and white blood cell for the more accurate way. A retrospective study of diagnostic peritoneal lavage was done of 90 patients with blunt abdominal trauma during 2 years from January, 1996 to December, 1997 and summerized our experience. The results were as follows; 1) There are 73 males and 17 females in sex. The most common type of injury was the traffic accident. 2) With many variable criteria of RBC and WBC, the accuracy and the false positive and false negative were not different in statistical results. The average accurracy was 80.1%. 3) The operations were done in 30(33.3%) cases, and the most frequent injuried organ was the small bowel, 20 cases(66.7%).
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이석희,이상은,박종근,문성배 부산대학교 사범대학 과학교육연구소 2001 科學敎育硏究報 Vol.28 No.-
The purpose of this study is to examine the effects of the application of experimental practices, descriptive tests, and research papers among the performance assessments on the students' accomplishment, their scientific research abilities and their attitudes to science in high schools. Two classes among the first grade ones were selected and assigned to the control group and the experimental group respectively. T-analysis of pre-test showed that the two groups have the same quality(p>.05). The questionnaire made by the Korean Educational Development Institute(KEDI) was used. Its reliability is .689. The experimental process was evaluated on the basis of the criterion table and reports. T-tes and ANOVA were enforced with the SPSS/WIN(ver10.0) for the purpose of analyzing the mean difference between the two groups. The results of this study are as follows. First, though the application of the performance assessments showed that there were little significant differences between the scientific research abilities of the experimental group with the experimental practice and those of the control group(p>.05), and the results of the pre-test and the post-test on the experimental group revealed that there were significant effects on improving the students' scientific research abilities(p<.05). Second, the result of the test using ANOVA at each research course factor showed that there was no significant difference at most research course factors that there were significant differences between the two groups in expanding the scientific research abilities of classification and measurement(p<.05). Finally, the results of the pre-test and the post-test by application of the performance assessments showed that they had significant effects on changing the students' attitude to science subject positively(p<.05). In addition, the results of the pre-testing and the post-testing on the sub-factors of the attitude related to science showed that there were significant changes in the concept of 'self-consciousness' and the attitude to learning(p<.05). In conclusion, the application of the performance assessments has positive effects on improving the high school students' scholastic achievement, scientific research abilities, and attitudes to related to science. Consequently, science teachers should be willing to use various performance assessments in the class.
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