서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

경막내 척수외 종양의 수술적 치료

신병준,이재철,윤태경,정호원,한성우,김동원,김경제,김연일 대한척추외과학회 2002 대한척추외과학회지 Vol.9 No.3

파킨슨씨병에서 Selegiline HCI (MAO-B^(�))의 효과 및 안전성

조기현,김명규,김태선,김병채,이승한,황인용,김준태,김종기 全南大醫大 2000 전남의대학술지 Vol.36 No.2

고콜레스테롤혈증 환자에서 Atorvastation의 유용성 및 안전성에 관한 연구

고종훈,신준한,김한수,탁승제,최병일,김동수,권혁문,김현승 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.9

Distribution of Aquaporins (Water Channels) in the Rat Salivary Glands

정지연(Ji-Yeon Jung),한창룡(Chang-Ryoung Han),정연진(Yeon-Jin Jeong),오원만(Won-Mann Oh),김미원(Mi-Won Kim1),김선헌(Sun-Hun Kim),김옥준(Ok-Joon Kim),김현진(Huyn-Jin Kim),고정태(Jeong-Tae Koh),최홍란(Hong-Ran Choi),김원재(Won-Jae Kim) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.3

침샘은 하루 1.5 l의 침을 분비하며, 일반적으로 침샘에서 수분이동은 능동적 염분 이동에 따른 삼투현상에 의해 일어난다. 따라서 침샘에서 높은 수분투과성으로 인해 수분통로 단백인 aquaporin (AQP)들이 풍부하게 존재할 수 있다. 지금까지침샘에 4가지 형태의 AQP이 존재한다고 알려져 있지만 침샘에서 정확한 위치와 수분 이동에 대한 각 AQP들의 역할은 아직 확실치 않다. 본 연구는 300 g 정도의 흰쥐를 pentobarbital sodium (50 mg/kg, IP) 마취하에서 carbarchol (10 μg/kg)을 복강 내 투여한 후 AQP들의 역할과 분포를 면역조직화학방법으로 조사하였다. AQP1은 침샘의 미세혈관의 내피세포와 샘세포와 샘관 주위에 존재하는 근육상피세포에 존재하였다. AQP4는 침샘관의 마지막 부위인 배출관에 존재하였다. AQP5는 주로 장액세포의 바닥가쪽과 세포사이 분비소관을 포함한 세포꼭대기쪽막 에 존재하였으며 콜린성 분비 자극으로 세포꼭대기쪽막으로 이동되어 밀집되었다. AQP5는 모든 침샘의 사이관과 줄무늬 관에도 존재하였으며 점액세포는 약하게 존재하였다. AQP8은 AQP5처럼 장액세포의 바닥쪽막과 세포사이 분비소관을 포함한 세포꼭대기쪽막에 존재하였으며 콜린성 침분비 자극으로 세포꼭대기쪽막쪽으로 이동되어 밀집되었다. 이상의 실험결과는 AQP5와 AQP8이 장액세포에서 일차 침 형성시 일어나는 수분이동의 주된 통로임을 시사하였다. The salivary glands produce 1.5 l of fluid per day. As in other organs, the general paradigm in the salivary glands is that water movement occurs secondary to osmotic driving forces created by active salt transport. Therefore, high water permeability in salivary glands is expected to need a variety of aquaporin (AQP), a water channel. Although four AQPs have been known to reside in salivary glands, the precise location and roles of AQPs have been not well examined. This study is aimed to investigate the distribution of AQPs in 3 major salivary glands and their changes after cholinergic stimulation using immunohistochemical study in Sprague Dawley rats weighing 300 g under pentobarbital sodium anesthesia. AQP1 was localized in the endothelial cells of all salivary capillary vessels and the myoepithelial cells. AQP4 was demonstrated in the epithelium of the excretory ductal cells of all salivary glands. AQP5 and 8 were abundantly present in the basolateral membrane and apical membranes of the serous acini including intercellular secretory canaliculi, whereas AQP5 was weakly present in mucous acini. In addition, AQP5 was found in the epithelium of the intercalated and striated ducts. Upon stimulation of carbachol (10 μg/kg, I.P). AQP5 and 8 tended to translocate from basolateral membrane to the apical membrane, appearing as clusters of dots. These results suggest that AQP5 and 8 are the candidate molecules responsible for the water movement in salivary acinar cells.

기체온도 변화에 따른 질소레이저의 출력특성

김성훈,양준묵,추한태,이치원 충남대학교 자연과학연구소 1993 忠南科學硏究誌 Vol.20 No.2

While the temperature of the gas was being changed by modifying the value of E/P which being effects the output characteristics of gas laser into a temperature function, the optimum value of E/P was calculated, being applied to Nitrogen laser and was compared with experimental values. The optimum operational characteristics by the variation of the nitrogen gas temperature is observed at 30 ℃ in gas temperature and 120 torr in gas pressure. Also the value of E/P is evaluated to be 423 V ㎝^-1torr^-1 which is 2.2 times as large as that of theoretical value. According to increasing the nitrogen gas temperature, the nitrogen gas pressure for the laser action is to be broaded. However in this case, the maximum output power is slightly decrease.

자매에서 발생한 중증 마이코플라즈마 폐렴에서 스테로이드 치료

김도희,이경일,김명숙,윤유숙,황자영,임정우,강진한,이준성 대한감염학회 2009 감염과 화학요법 Vol.41 No.3

Numerous evidences support the pathogenesis that M. pneumonias pneumonia is associated with cell-mediat-ed immune reaction. We report 2 cases of severe M. pneumoniae pneumonia in previously healthy sisters, who were both admitted during an epidemic of M. pneumoniae pneumonia. The elder sister, who was 16 years old, was admitted with M. pneumoniae pneumonia. She showed no response to treatment with clarithroycin and levo-floxacin, and eventually progressed to severe ARDS, requiring mechanical ventilation. After treatment with hydrocortisone (200 mg/day), there were rapid improvements in clinical manifestations and chest radiographic findings. The younger sister, who was 14 years old, was admitted 10 days later, Presenting with fever but no pneumonic lesions on chest radiograph. Just like her sister, the infection showed no response to clarithromycin. Fever persisted and pneumonic consolidation with mild pleural effusion was noticed in the left lower lobe on the 5th hospital-day. After treatment with oral prednisolone (1 mg/kg), she showed rapid defervescence and on the 8th hospital day, no pneumonic lesions were detectable on chest radiograph. Given the fact that the pathogenesis of pneumonia and extrapulmonary manifestations in M. pneumoniae infection is immune-mediated, an immuno-suppressive therapy would be validated for selected patients with M. pneumoniae infections.

韓國의 肝吸蟲症에 관한 臨床的 硏究

金名世,李駿商,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1

For understanding of the significance on clinical features of clonorchiasis, the authors carried out the examinations for hematology, blood biochemistry and clinical symptoms on the patients whose stools were positive eggs of clonorchis sinensis in several endemic areas in Korea. A total of 287 clonorchiasis patients were examined with the stools by Stoll's egg counting method for the intensity of infection. The patients were divided into light, moderate Ⅰ & Ⅱ, heavy and very heavy infection groups depending upon the number of eggs per gm, of feces(EPG). All the calculated mean values for each items with standard deviation of hematological and blood bichemical tests were compared with those of the normal values and the detailed clinical symptoms according to the intensity of infection were analyzed. The results obtained are as follows: 1. The mean values of hematologic findings in each group of infection were within the normal limit except eosinophil counts. In the changes of hematological values by means of intensity of infection, the mean values of hemoglobin, hematocrit, RBC, WBC and eosinophil counts were increased in accordance with the intensity of infection. 2. To observe the occurrence of anemia in the patients infected with C. sinensis, the mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were compared in each group of infection. But all the values were shown within normal value. 3. All the mean values of blood biochemistry in each group of infection were within the normal limit. However the mean values of GPT, GOT, alkaline phosphatase, bilirubin and cholesterol were increased in accordance with the intensity of infection. Whereas, the mean values of total protein, albumin and A/G ratio were decreased. 4. The subjective symptoms of human clonorchiasis were increased by the higher intensity of infection. Among chief complaints, asthenia, lassitude and mental depression were most common as generalized symptoms, but as gastrointestinal symptoms, abdominal discomfort, epigastric pain and indigestion were most frequent. In physical examination, hepatomegaly, epigastric tenderness and jaundice were found most frequently. Even in the light infection group, over 60 per cent of patients complained the subjective symptoms. 5. With the above results, the changes in the fields of hematology, blood biochemistry and the clinical symptoms by means of intensity of infection were discussed for the specific clinical features of clonorchiasis.

肝카필라리아症(Hepatic Capillariasis)에 있어서 卵周圍 沈降反應에 관한 實驗的 硏究

金正俊,李駿商,林漢鍾 고려대학교 의과대학 1982 고려대 의대 잡지 Vol.19 No.1

The present study was undertaken to investigate the circumoval precipitation (COP) reaction of Capillaria hepatica. The source of C. hepatica eggs was the deposit of nonembryonated eggs encapsulated in the liver of the house rat. The eggs were embryonated in the incubator at 27℃ to 30℃ for four to five weeks. Rat was infected orally with 2,000 embryonated eggs. Blood collections were made by the cardiac puncture after infection of C. hepatica for 10 to 63 days. Fresh eggs obtained from the liver of the house rat washed three times with distilled water and lyophilized with the freeze dryer. lyophilization was carried out on a temperature of -50℃ and a vacuum of 10^(-4)㎜Hg. The occurrence of circumoval precipitation (COP) reaction was proved by the embryonated eggs of C. hepatica which were incubated in the sera from rats given embryonated eggs of C. hepatica by orally. The result findings were as follows: 1. The precipitations were formed around the mucoid plugs of the embryonated eggs after 24 hours at 37℃ by the sera from infected rats. 2. The average positive rates of COP reaction in different diluting solutions were 46.5% in PBS, 35.5% in Millonig's buffer solution, 20.2% in normal saline and 8.8% in distilled water. 3. Precipitations in COP reaction was noticed even in highly diluted sera up to 1 : 128. The high positive rate of COP reaction in serum with the PBS diluting solution was shown to be over 76% at the dilution of 1 : 16 to 1:32. 4. There were no differences in the positive rates of the COP reaction between the fresh and lyophilized eggs using PBS as a diluting solution. 5. There were no differences in the positive rates of COP reaction using lyophilized embryonated eggs in each serum collected during the 10th to 63rd days after infection of C. hepatica. 6. With the embryonated eggs of C. hepalica precipitations were not produced in the sera from rats infected with Clonorchis sinensis, Metagonimus yokogawai, Paragonimus iloktsuenensis and by the sera from human infected with Ascaris lumbricoides.

전산모형과 지리정보시스템을 결합한 Wed 기반의 유역 및 하수도 관리시스템의 개발

김준현,박형춘,한영한 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.A

A Web based watershed and sewer management system was developed for the analysis of stormwater runoff and sewer flow, and for optimal operation of sewer works using ArcView and SWMM. SWMM and ArcView were dynamically linked together using Avenue and Visual Basic in order to construct user-friendly management system. The developed system was applied to Choonchun city to verify its utilities. All the relevant field data were analyzed on the basis of developed system, and the modeling of runoff and sewer flow was implemented using RUNOFF and TRANSPORT blocks in SWMM. This system was connected to the management system of surface and subsurface environment management system in order to develop an integrated environmental mangament system. Futhermore. this system will be a critical part of overall control system of sewer works including sewer line and wastewater treatment plant. As this system can provide comprehensive prediction of flow and pollution profiles and analytical tool equipped with Web-GIS, it could serve widely as a tool not only for optimal mangament, but also for decision support system to examine the efficiency of planning and implementation of sewer projects.