Vascular Protective Role of Samul-Tang in HUVECs: Involvement of Nrf2/HO-1 and NO

Choi, Eun Sik,Lee, Yun Jung,Seo, Chang Seob,Yoon, Jung Joo,Han, Byung Hyuk,Park, Min Cheol,Kang, Dae Gill,Lee, Ho Sub Hindawi Publishing Corporation 2016 Evidence-based Complementary and Alternative Medic Vol.2016 No.-

<P>Samul-Tang (Si-Wu-Tang, SMT), composed of four medicinal herbs, is a well-known herbal formula treating hematological disorder or gynecologic disease. However, vascular protective effects of SMT and its molecular mechanisms on the vascular endothelium, known as the central spot of vascular inflammatory process, are not reported. The aim of this study was to investigate vascular protective effects of SMT water extract in human umbilical vein endothelial cells (HUVECs). Water extract of SMT was prepared and identified by HPLC-PDA analysis. Expression of cell adhesion molecules (CAMs) and heme oxygenase-1 (HO-1) and translocation of nuclear factor-kappa B (NF-<I>κ</I>B) and nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined by western blot. Nuclear localization of NF-<I>κ</I>B and Nrf2 was visualized by immunofluorescence and DNA binding activity of NF-<I>κ</I>B was measured. ROS production, HL-60 monocyte adhesion, and intracellular nitric oxide (NO) were also measured using a fluorescent indicator. SMT suppressed NF-<I>κ</I>B translocation and activation as well as expression of CAMs, monocyte adhesion, and ROS production induced by TNF-<I>α</I> in HUVECs. SMT treated HUVECs showed upregulation of HO-1 and NO which are responsible for vascular protective action. Our study suggests that SMT, a traditionally used herbal formula, protects the vascular endothelium from inflammation and might be used as a promising vascular protective drug.</P>

제통단이 퇴행성 슬관절염 환자의 삶의 질에 미치는 영향

서병관,류성룡,강중원,우현수,이상훈,이재동,최도영,김건식,이두익,이윤호 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objectives : to evaluate the effects of Jetongdan, the newly developed herbal medicine, on the quality of life in patients with osteoarthritis of knee Methods : Placebo-controlled, randomized clinical trial on the 80 patients with osteoarthritis of the knee was fulfilled. After enrollment, they took medication as they enrolled. And they were asked to answer the questionnaires (Korean Health Assessment Questionnaire (KHAQ), Lequesne's functional index (LF1), visual analogue scale (VAS)) and analysed with the erythrocyte sedimentation rate (ESR) at baseline, after 4 weeks and 8 weeks of medication. Results : Total KHAQ score, some categories of KHAQ like hygiene, activities were significantly improved after 8 weeks of medication. And the change of the KHAQ score was significantly correlated with the change of VAS. But LFI, VAS, ESR level was not significantly improved, and it was considered because of the short medication period and small size of study population. Conclusion : In conclusion, Jetongdan could improve the health-related quality of life in patients with osteoarthritis of knee. Further study in the large population, in long period were recommended.

분산 객체 실시간 스트리밍 기법

서봉근,김윤호,이정환 국립7개대학공동논문집간행위원회 2004 공업기술연구 Vol.4 No.-

In this paper, We present a technique for live streaming using RMI and JMF. We handle live streaming using JMF that based on RTP in distributed computing environment using RMI. The media transmission is dispersed under distributed environment using. RMI with it will be able to solve the media service concentration of server and it will be able to provide the service which is flexibility. Also, It uses the media transmission function of JMF which solves the absence of object exchange function when using RMI. And, it is implemented with 100% pure Java using RMI and JMF.

퇴행성 슬관절염에 대한 제통단의 안정성과 효능 임상연구

서병관,류성룡,강중원,안경애,이상훈,최도영,김건식,이두익,이윤호,이재동 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objectives : In order to investigate the efficacy and safety of the newly developed herbal medicine Jetongdan, a placebo-controlled randomized clinical trial of patients with osteoarthriotis of the knee was undertaken. Methods : Data were obtained from 80 patients with OA of the knee. After the enrollment, they were asked to answer a disease-specific questionnaire (Western Ontario and McMaster Universities (WOMAC) OA index) and analyzed with the erythrocyte sedimentation rate (ESR) in order to evaluate the efficacy of Jetongdan, and analyzed for aspartate transaminase (AST) level, alanine transminase (ALT) level, blood urea mitrogen (BUN) level, and creatinine (Cr) level in order to evaluate the safety of Jetongdan. Results : The liver function and renal function did not deteriorate after treatment with Jetongdan. Composite WOMAC score and physical function subscale was improved, but pain subscale, stiffness subscale, and ESR were not improved by Jetongdan. This was possibly because the baseline characteristics of the two groups were not homogenized after randomization. Conclusions : Jetongdan could be a promising treatment option for osteoarthritis of the knee. Further study in a larger population with appropriate severity grades is recommended.

가시광을 이용한 ZnS/ZnO 상에서의 광촉매 반응에 의한 물로부터의 수소생성

서승광,윤필상,이한수,이호인 한국공업화학회 2003 응용화학 Vol.7 No.2

The pure ZnS can not absorb visible light because of its large band gap of 3.6eV. The ZnO/ZnS was obtained from partial oxidation of ZnS, and its band gap was 2.5eV where visible light can be absorbed. From XRD analysis, it was confirmed that only ZnO was formed during oxidation process but not ZnSO₄. In case of ZnO/ZnS, the quantum efficiency for the hydrogen evolution from water was 0.87% under visible light irradiation. In order to improve the interaction between ZnO and ZnS, ZnS/ZnO was prepared by precipitating ZnS on ZnO. After calcination of ZnS/ZnO, the band gap became narrow. The pure ZnO and the physically mixed ZnS-ZnO could not absorb visible light, but the ZnS-doped ZnO (ZnS/ZnO) could do. The band gap of pure ZnO was 3.2 eV and that of ZnS/ZnO was 2.5ev. The best activity was obtained when clcination temperature and time were 500 ℃ and 1 h, respectively. The quantum efficiency of ZnS/ZnO for the reaction was 3.29%.

퇴행성 슬관절염 환자의 증상 중증도 지표와 DITI의 상관성 연구

서병관,류성룡,강중원,안경애,이재동,최도영,김건식,이두익,이윤호,이상훈 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objectives : To investigate the applicability of thermography as severity measurement in the patients with osteoarthritis (OA) of the knee. Methods : Data were obtained from 80 patiens with OA of the knee. They were asked to answer two disease-specific questionnaire (Western Ontario and McMaster Universities (WOMAC) OA index, Lequesne's Functional Index (LFI)), one generic instrument (Korean Health Assessment Questionnaire (KHAQ)), VAS in order to assess the severity of disease, quality of life, degree of pain and taken thermography in standardized environment. Results : The thermal difference between ipsilateral side and contralaterla side of lateral aspect of knee was correlated with that of medial aspect of knee, and the thermal difference of anterior thigh was correlated with that of lateral, medial aspect of knee and patella region. Age, duration of disease, duration of morning stiffness, sex, crepitus, and painful side of knee were not correlated with the thermal differences of each region. LFI, WOMAC, WOMAC pain subscale, WOMAC stiffness subscale, WOMAC physical function, KHAQ,, VAS were not correlated with the thermal differences of each region. Conclusion : Further study on the thermography on OA of the knee in population with appropriate severity grade and the standardization of analysis of thermographic data were recommended.

NaCl 처리에 대한 벼 Callus 의 품종간 양이온함량 차이

윤주,신서호,이영만 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

Callus 현탁배양시 배양기간, NaCl 농도를 달리한 양이온 함량의 내염성 품종과 감수성 품종간 변화에서 callus 증식율은 NaCl 이 첨가된 배지에서 현탁배양 3주후 감수성 품종과 내염성 품종간 차이를 보이지 않지만, 6주, 9주 후에는 내염성 품종인 상해향혈나가 감수성 품종인 동진벼보다 NaCl 농도 증가에 따른 감소율이 훨씬 적게 나타났다. callus의 양이온 함량은 두 품종 모두에서 NaCl 농도가 증가함에 따라 ??, ??/??비율은 증가하나, ??, ??, ?? 이온은 감소하는 경향을 나타냈다. In vitro culture of rice(Oryza sativa L.), cation contents were different between the genotypes of tolerant and susceptible to NaCl treatment. Differences in callus proliferation between salt tolerant and susceptible genotypes due to NaCl content in culture media were not observed until 3 weeks of culture, but became clear 6 to 9 weeks after culture. That is, decrease in calluse growth due to increasing of NaCl content in the media was much lower in tolerant genotypes than in susceptible genotypes. Content of ?? and ??/?? ratio in callus increased, as NaCl concentration in medial increased, while that of ??, ?? and ?? decreased in tolerant as well as susceptible genotypes.

성인 음낭수종의 치료로서 Window Operation의 효용성

서병욱,박영호,이남규,전윤수,김민의,김영호,송윤섭,구자현 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

We intended to determine the usefulness of window operation for adult hydrocele repair. We reviewed medical records of 132 patients from January 1995 to June 1998 with primary hydrocele and evaluated complications and recurrence rates of eversion and/or excision, plication, and internal drainage technique. Recurrence rate was highest in internal drainage technique as 85.2%, and those of eversion and/or excision and plication technique were 1.3% and 4%, respectively. Complication rate was highest in eversion and/or excision technique, and lowest in internal drainage technique. Although complication rate was lowest in window operation, this operation could not be successful due to high recurrence, and we suggest that this operation should be abandoned for hydrocele repair.

20세기 가면극과 인형극의 예능전수과정 연구

서연호 고려대학교 한국학연구소 1997 한국학연구 Vol.9 No.-

편마비 환자의 총대퇴동맥에서 초음파를 이용한 혈류 동태 분석

김완호,고명환,서정환,김연희 大韓再活醫學會 1998 Annals of Rehabilitation Medicine Vol.22 No.3