White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment

Zhu Qinchao,Zhou Xuhao,Zhang Yanan,Ye Di,Yu Kang,Cao Wangbei,Zhang Liwen,Zheng Houwei,Sun Ziyang,Guo Chengchen,Hong Xiaoqian,Zhu Yang,Zhang Yajun,Xiao Ying,Valencak Teresa G.,Ren Tanchen,Ren Daxi 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background : Post-traumatic massive hemorrhage demands immediately available first-aid supplies with reduced operation time and good surgical compliance. In-situ crosslinking gels that are flexibly adapting to the wound shape have a promising potential, but it is still hard to achieve fast gelation, on-demand adhesion, and wide feasibility at the same time. Methods : A white-light crosslinkable natural milk-derived casein hydrogel bioadhesive is presented for the first time. Benefiting from abundant tyrosine residues, casein hydrogel bioadhesive was synthesized by forming di-tyrosine bonds under white light with a ruthenium-based catalyst. We firstly optimized the concentration of proteins and initiators to achieve faster gelation and higher mechanical strength. Then, we examined the degradation, cytotoxicity, tissue adhesion, hemostasis, and wound healing ability of the casein hydrogels to study their potential to be used as bioadhesives. Result : Rapid gelation of casein hydrogel is initiated with an outdoor flashlight, a cellphone flashlight, or an endoscopy lamp, which facilitates its usage during first-aid and minimally invasive operations. The rapid gelation enables 3D printing of the casein hydrogel and excellent hemostasis even during liver hemorrhage due to section injury. The covalent binding between casein and tissue enables robust adhesion which can withstand more than 180 mmHg blood pressure. Moreover, the casein-based hydrogel can facilitate post-traumatic wound healing caused by trauma due to its biocompatibility. Conclusion : Casein-based bioadhesives developed in this study pave a way for broad and practical application in emergency wound management.

Research on the Dynamic Evolution of Manufacturing Industry Cluster Network-The Perspective of the Synergy of Core Enterprise Transformation and Cluster Members

Ye Di,Liu Zheng Yu,Lin Feng,Zhao Kai 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.10

China’s traditional industry clusters meet challenges and opportunities as the global economy goes ups and downs. Following the trend of industry upgradation is a highlight in both theoretical and practical domain. This paper focuses on the principles of dynamic evolution of China’s industry clusters in order to pursuit and establish its enduring growing drive and competitive advantages. This paper gives research into dynamic evolution of cluster network, centering on its inherent relationship with the core enterprise transformation and the restructure flexibility of cluster members and defines “the transformation and up gradation of the core enterprises in cluster network”, finds out the dynamic and persistent effect of transformation and upgradation of the core enterprises on network resources and puts forward the dilemma of the dynamic evolution and upgradation of China’s cluster network.

Long noncoding RNA atlas of the inflammation caused by asthma in mice

Ye Chen,Shou‑di He,Xiao‑dong Li,Zhi‑li Hu,Chao Zhang,Feng Xu 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.4

There is little evidence regarding the roles oflong noncoding RNAs (lncRNAs) in inflammation caused byasthma. In this study, we successfully generated an asthmamouse model that was induced by ovalbumin (OVA). Theeffects of dexamethasone (Dex) treatment on lung tissuewere investigated using pathological and biochemicalmethods, including Diff-Quik staining, enzyme-linkedimmunosorbent assay (ELISA), hematoxylin–eosin (H&E)staining, and western blotting (WB). The inflammation waseffectively relieved with Dex treatment. High-throughputsequencing revealed that a total of 1490 lncRNAs were detected in lung tissue samples. Differential expressionanalysis revealed that the Dex group had 20 upregulatedand 15 downregulated lncRNAs compared with those inthe Model group. Moreover, nine differentially expressedand inflammation-related lncRNAs were verified by quantitativereal-time reverse transcription polymerase chainreaction (qRT-PCR). Furthermore, the regulation networksof these nine lncRNAs, their potential binding microRNA(miRNAs), and the putative target genes showed that theselncRNAs play important roles in the nuclear factor kappalight-chain-enhancer of activated B cells (NF-κB) signalingpathway. We further identified the expression levels of threepotential binding miRNAs by qRT-PCR. The results of thisstudy contribute to a better understanding of the functionsof lncRNAs in inflammation caused by asthma.

Effect of Vacuum Carburizing Time on Microstructure and Mechanical Properties of Tantalum Carbide Layer

Chongqing Di,Xiaodong Yan,Xuming Lv,Chao Yan,Wei Ye,Defu Li 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

A (TaC/Ta2C) carbide bilayer is obtained by vacuum carburizing technology on the surface of Ta substrate at 1673 K for 4,8, and 12 h. XRD, SEM and EBSD are utilized to investigated phase composition and the microstructure. The mechanicalproperties of the Ta and tantalum carburized materials are studied with Vicker’s hardness tester and nanoindenter, adhesionautomatic scratch tester, reciprocating friction and wear testing machine. The results show that the outside surface phasecomposition of the carbide bilayer is all the TaC phase. With the increase of the carburizing time from 4 to 12 h, the averagegrain size from approximately 500 nm to 10 μm, the thickness of the carbide bilayer is from 11 to 20 μm. The microhardnessincreases from 104.1 to 322.5 HV, and the elastic modulus are from 466.6 to 615.3 GPa. Adhesive strength is best at 8 h,49.1 N, compared to 19 N at 4 h and 36.5 N at 12 h. The friction and wear coefficient of Ta fluctuates significantly between0.6 and 1.0, after carburizing treatment, the friction and wear coefficient fluctuates smoothly, and the wear resistance is wellimproved.

Development and validation of risk score for predicting spontaneous rupture of hepatocellular carcinoma

Feng Ye,Di Ma,Xiao-Yong Gong,Yu-Chen Yang,Yong-Jun Chen 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.99 No.5

Purpose: Spontaneous rupture is a potentially serious complication of liver cancer. A risk score was developed and validated for predicting spontaneous rupture based on a retrospective study. Methods: Multiple logistic regression analysis was used to study the relationship between clinical variables and spontaneous rupture. The independent rupture predictors were converted into a score based on the odds ratio. Predicted attributes of the developed scores were then verified using a dataset in 2019. Results: The incidence of spontaneous rupture was 5.5% from 2002 to 2019. A 10-point score (α-FP of ≥400 μg/L, 1; protrusion from liver surface, 2; ascites, 3; tumor size of >5 cm, 4) was derived for prediction of rupture and area under the receiver-operating characteristic curve was 0.9 (95% confidence interval, 0.87–0.92). When applying a cutoff value of 5 points or more, the specificity was 0.87 and the sensitivity was 0.84. A validation cohort consisting of 202 hepatocellular carcinoma patients reproduces the predictive, identification, and calibration characteristics. The observed rate of spontaneous rupture according to risk stratification of the score was 0.6% for those with a score of 0–4, 21.6% for a score of 5–7, and 36.4% for a score of 8–10 in the validation cohort. Conclusion: Here, based on routine clinical data, we determine the factors that affect prognosis and propose an effective tool for predicting spontaneous rupture, which may be useful in guiding priority treatment of high-risk patients or clinical routine preventive treatment.

Evolution of Microstructures and Compressive Properties in Al0.5CrFeNi2.1Mn0.8Tix High Entropy Alloys

Xu Chen,Di Gao,Yan Zhang,Jia Xuan Hu,Ye Liu,Feng Xiang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.1

The effects of Ti on the microstructures, macrohardness and compressive properties of the as-cast Al0.5CrFeNi2.1Mn0.8TixHEAs were investigated. The results showed that the microstructures of as-cast Al0.5CrFeNi2.1Mn0.8Tixalloys was changedfrom FCC phase to a mixture of FCC and BCC phases, then to a mixture of BCC phase and Ti-containing intermetalliccompound as the increasing of Ti content. Chrysanthemum-like eutectic microstructure was obtained in theAl0.5CrFeNi2.1Mn0.8Ti0.5and Al0.5CrFeNi2.1Mn0.8Tialloys. The area of flower core was composed of BCC2 phase, andeutectic microstructure was achieved in the petal area which contained BCC1 phase and BCC2 phase. Moreover, the macrohardnessof the as-cast alloys increased with the increasing of Ti, and the Al0.5CrFeNi2.1Mn0.8Ti0.5alloy showed excellentcomprehensive compressive properties.

Evolution of Microstructures and Properties in AlxCrFeMn0.8Ni2.1 HEAs

Xu Chen,Di Gao,Jia Xuan Hu,Ye Liu,Chang Ping Tang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.5

The microstructures, compression and corrosion behaviors of the as-cast AlxCrFeMn0.8Ni2.1high-entropy alloys (0 ≤ x ≤ 2.3)were investigated in this paper. It was found that the crystal structure changed from initial dual FCC structure to mixed FCCand BCC structure, then to BCC structure as the increasing of Al content. Al0.8CrFeMn0.8Ni2.1alloy exhibited a typicalspinodal structure consisting of alternating two phases microstructure. Moreover, sunflower-like microstructure was obtainedin the as-cast AlxCrFeMn0.8Ni2.1alloys (1.0 ≤ x ≤ 2.0). With the increasing of Al, the macrohardness increased while the plasticitydecreased in the alloys. The addition of an appropriate amount of Al could improve the compressive fracture strengthof the alloys. In addition, the corrosion resistance deteriorated slightly with the increasing of Al in 1 mol/L NaCl solution.

Efficiency Optimization of Variable Iron Loss Resistance Asynchronous Motor Based on Grey Wolf Optimization Algorithm

Guo Zhen,Tong Di,Zhao Yan-cheng,Chen Shuang,Nai Ji-qiu,Ye Ming-hao 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

Asynchronous motor is widely used in various industrial fields. When asynchronous motor works in case close to the rated load, it will run at maximum efficiency. However, most of the load is 50–100% of rated load and it will lead to the serious waste of energy. This paper proposes an efficiency optimization control strategy based on the grey wolf optimization algorithm to improve the operating efficiency of asynchronous motors at light loads. The motor loss model considering the change of iron loss is established and it makes the asynchronous motor model more accurate. The grey wolf optimization algorithm is used to find the optimized flux value. When the asynchronous motor operates at the optimal flux, the loss power of the motor is decreased and the operation efficiency improves effectively. The proposed method reduces the flux search time and improves the stability of the system. The simulation model is established and the simulation results are provided to verify the feasibility of the proposed control strategy achieving the global efficiency optimum of asynchronous motor.

Research of Circuit Working Construction Elevator with Single-guide Rail and Multi-cages

Kun Zhang,Kaiqiang Wang,Di Li,Qing Sun,Zhen Ye,Wei Liu Council on Tall Building and Urban Habitat Korea 2022 International journal of high-rise buildings Vol.11 No.2

As one of the most important vertical transportation equipment in super high-rise buildings, the construction elevator directly affects the project period, cost, and effectiveness. The paper proposes a new construction elevator with single-guide rail and multi-cages. It can solve the problems of single construction elevator capacity shortage and efficacy decrease with height reduction, the occupancy of plan and elevation position of multiple construction elevators, and extension of total construction period by cycling operation of multi-cages on a single-guide rail. The paper focuses on the design and research of the main components of the equipment, such as the rotating guide rail mechanism, vertical bearing mast tie system, segmented electrical power supply system, group control scheduling system, and safety anti-collision system.

Genome-wide identification of WRKY family genes and their response to abiotic stresses in tea plant (Camellia sinensis)

Pengjie Wang,Chuan Yue,Di Chen,Yucheng Zheng,Qian Zhang,Jiangfan Yang,Naixing Ye 한국유전학회 2019 Genes & Genomics Vol.41 No.1

The WRKY transcription factors (TFs) family is one of the largest TF families in plants and plays a central role in diverse regulation and multiple stress responses. However, the systematical analysis of the WRKY gene family in tea plant (Camellia sinensis) based on genomic data has been lacking. The primary objective of this study was to set a systematic analysis of the WRKY gene family based on genomic data in tea plant and analyze their expression profiles under various abiotic stresses. We searched the tea plant genome using the consensus model of the WRKY domain (PF03106) and then used these search results to identify all the WRKY family members by SMART and the CDD program. Analyze their phylogeny, classification, structure, conserved motifs, Cis-elements, interactors and expression profiles. 56 putative WRKY genes were identified from the tea plant genome and divided into three main groups (I–III) and five subgroups (IIa–IIe) according to the WRKY domains and the zinc-finger structure. The gene structure and conserved motifs of the CsWRKY genes were also characterized and were consistent with the classification results. Annotation analysis showed that 34 CsWRKY genes may be involved in stress responses. Promoter analysis implied that CsWRKY genes, except for CsWRKY55, possessed at least one abiotic stress response cis-element. Expression profiles of CsWRKY genes in different tissues were analyzed with RNA-seq data. The results showed that 56 CsWRKY genes had differential expression in their transcript abundance. The expression profiles also showed that many identified CsWRKY genes were possibly involved in the response to cold, drought, salt, or ABA treatment. Tea plant genome contains at least 56 WRKY genes. These results provide useful information for further exploring the function and regulatory mechanism of CsWRKY genes in the growth, development, and adaption to abiotic stresses in tea plant.