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        Aero-elastic wind tunnel test of a high lighting pole

        Yaozhi Luo,Yucheng Wang,Jiming Xie,Chao Yang,Yanfeng Zheng 한국풍공학회 2017 Wind and Structures, An International Journal (WAS Vol.25 No.1

        This paper presents a 1:25 multi-freedom aero-elastic model for a high lighting pole at the Zhoushan stadium. To validate the similarity characteristics of the model, a free vibration test was performed before the formal test. Beat phenomenon was found and eliminated by synthesis of vibration in the X and Y directions, and the damping ratio of the model was identified by the free decay method. The dynamic characteristics of the model were examined and compared with the real structure; the similarity results were favorable. From the test results, the major along-wind dynamic response was the first vibration component. The along-wind wind vibration coefficient was calculated by the China code and Eurocode. When the peak factor equaled 3.5, the coefficient calculated by the China code was close to the experimental result while Eurocode had a slight overestimation of the coefficient. The wind vibration coefficient during typhoon flow was analyzed, and a magnification factor was suggested in typhoon-prone areas. By analyzing the power spectrum of the dynamic cross-wind base shear force, it was found that a second-order vortex-excited resonance existed. The cross-wind response in the test was smaller than Eurocode estimation. The aerodynamic damping ratio was calculated by random decrement technique and the results showed that aerodynamic damping ratios were mostly positive at the design wind speed, which means that the wind-induced galloping phenomenon is predicted not to occur at design wind speeds.

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        Aero-elastic wind tunnel test of a high lighting pole

        Luo, Yaozhi,Wang, Yucheng,Xie, Jiming,Yang, Chao,Zheng, Yanfeng Techno-Press 2017 Wind and Structures, An International Journal (WAS Vol.25 No.1

        This paper presents a 1:25 multi-freedom aero-elastic model for a high lighting pole at the Zhoushan stadium. To validate the similarity characteristics of the model, a free vibration test was performed before the formal test. Beat phenomenon was found and eliminated by synthesis of vibration in the X and Y directions, and the damping ratio of the model was identified by the free decay method. The dynamic characteristics of the model were examined and compared with the real structure; the similarity results were favorable. From the test results, the major along-wind dynamic response was the first vibration component. The along-wind wind vibration coefficient was calculated by the China code and Eurocode. When the peak factor equaled 3.5, the coefficient calculated by the China code was close to the experimental result while Eurocode had a slight overestimation of the coefficient. The wind vibration coefficient during typhoon flow was analyzed, and a magnification factor was suggested in typhoon-prone areas. By analyzing the power spectrum of the dynamic cross-wind base shear force, it was found that a second-order vortex-excited resonance existed. The cross-wind response in the test was smaller than Eurocode estimation. The aerodynamic damping ratio was calculated by random decrement technique and the results showed that aerodynamic damping ratios were mostly positive at the design wind speed, which means that the wind-induced galloping phenomenon is predicted not to occur at design wind speeds.

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        Genome-wide identification of WRKY family genes and their response to abiotic stresses in tea plant (Camellia sinensis)

        Pengjie Wang,Chuan Yue,Di Chen,Yucheng Zheng,Qian Zhang,Jiangfan Yang,Naixing Ye 한국유전학회 2019 Genes & Genomics Vol.41 No.1

        The WRKY transcription factors (TFs) family is one of the largest TF families in plants and plays a central role in diverse regulation and multiple stress responses. However, the systematical analysis of the WRKY gene family in tea plant (Camellia sinensis) based on genomic data has been lacking. The primary objective of this study was to set a systematic analysis of the WRKY gene family based on genomic data in tea plant and analyze their expression profiles under various abiotic stresses. We searched the tea plant genome using the consensus model of the WRKY domain (PF03106) and then used these search results to identify all the WRKY family members by SMART and the CDD program. Analyze their phylogeny, classification, structure, conserved motifs, Cis-elements, interactors and expression profiles. 56 putative WRKY genes were identified from the tea plant genome and divided into three main groups (I–III) and five subgroups (IIa–IIe) according to the WRKY domains and the zinc-finger structure. The gene structure and conserved motifs of the CsWRKY genes were also characterized and were consistent with the classification results. Annotation analysis showed that 34 CsWRKY genes may be involved in stress responses. Promoter analysis implied that CsWRKY genes, except for CsWRKY55, possessed at least one abiotic stress response cis-element. Expression profiles of CsWRKY genes in different tissues were analyzed with RNA-seq data. The results showed that 56 CsWRKY genes had differential expression in their transcript abundance. The expression profiles also showed that many identified CsWRKY genes were possibly involved in the response to cold, drought, salt, or ABA treatment. Tea plant genome contains at least 56 WRKY genes. These results provide useful information for further exploring the function and regulatory mechanism of CsWRKY genes in the growth, development, and adaption to abiotic stresses in tea plant.

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        MOF-Derived Porous CeO2-x/C Nanorods and their Applications in Uric Acid Biosensor

        Jinbao Luo,Jiewu Cui,Yan Wang,Dongbo Yu,Yongqiang Qin,Hongmei Zheng,Yu Hong,Yong Zhang,Yucheng Wu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.7

        Selectivity is significant to the practical applications of electrochemical biosensors in clinical and diagnostic field. In this paper, porous CeO2-x/C nanorods (NRs) derived from Ce-based metal organic framework (MOF) were synthesized and employed as substrate to construct uric acid biosensors with high sensitivity and selectivity at low working potential. The morphology, microstructures and elemental states of as-prepared samples were investigated by SEM, XRD, TEM and XPS systematically. It was found that a great amount of oxygen vacancies was introduced into the interstitial of CeO2 and nonstoichiometric CeO2/C (CeO2-x/C) nanorods based on Ce-MOF were formed under calcination in Ar atmosphere. The increased oxygen vacancies enabled the negatively shifting of the working potential towards H2O2 detection for CeO2-x/C nanorods, favoring the construction of biosensors based on the detection of H2O2. Uric biosensors based on CeO2-x/C NRs exhibited a high sensitivity of 220.0 μA · cm -2 · mM -1 and a linear range from 50 μM to 1000 μM at working potential of -0.4 V versus SCE. It also exhibited superior selectivity toward interferents coexisting with uric acid in urine due to the low working potential.

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