정식 시기와 적심 횟수가 화단국화의 생장과 개화에 미치는 영향

姚婧超, 정해준, 김동찬, 이진희, 권민훈, 서병기 배재대학교 자연과학연구소 2013 自然科學論文集 Vol.24 No.1

This study was conducted to investigate optimum planting date and pinching times on the growth and flowering of garden chrysanthemum(Dendranthema grandiflorum Ramat.). 3 lines of garden mums were planted on May 16, June 13 and July 11 respectively. As planting date was delayed, all the 3 lines' plant height and plant width was reduced. 09-19-49 line got the biggest plant height and width, followed by 09-11-37 line. And 08-166-02 line was the smallest. In different planting times, the experiment group of May 16 and June 13 got the biggest plant height, plant width and stem diameter. However, in the experiment group of May 16, most of the plants got flattened. The experiment group of June 13 got the best ornamental value, and the ornamental value was reduced in the experiment group of July 11 by the short vegetative growth. 09-19-49 line got the most flowers, while in different planting time, the experiment group of May 16 got the most flowers. 09-11-37 line got the biggest flower diameter. 09-11-37 line and 09-09-51 line were planted in the containers on the rooftop. Each groups were pinched by 1, 2 and 3 times on July 2, July 26 and Aug 16 respectively. There was no effect on plant height, plant width, and stem diameter of 09-09-51 line and 09-11-37 line between 1-time-pinching group and 2-time-pinching group. But 3-time-pinching group was reduced. There was no significance in flower diameter of these two lines but the number of flowers was reduced by more pinching times. Therefore, in this experiment, the groups which were planted on June 13 got the best ornamental value and the recommended planting time is in the middle of June. Furthermore, 2 times of pinching was recommended.

Adsorptive Granulocyte and Monocyte Apheresis in the Treatment of Ulcerative Colitis: The First Multicenter Study in China

( Ya-min Lai ),( Wei-yan Yao ),( Yao He ),( Xuan Jiang ),( Yu-bei Gu ),( Min-hu Chen ),( Yu-lan Liu ),( Yao-zong Yuan ),( Jia-ming Qian ) 대한소화기학회 2017 Gut and Liver Vol.11 No.2

Background/Aims: Patients with active ulcerative colitis (UC) have elevated levels of activated myeloid-derived leukocytes as a source of inflammatory cytokines. The selective deple-tion of these leukocytes by adsorptive granulocyte/monocyte apheresis (GMA) with an Adacolumn should alleviate inflammation, promote remission and enhance drug efficacy. However, studies have reported contrasting efficacy out-comes based on patients` baseline demographic variables. This study was undertaken to understand the demographic features of GMA responders and nonresponders. Methods: This was a multicenter study in China involving four institutions and 34 patients with active UC. Baseline conventional medications were continued without changing the dosage. The treatment efficacy was evaluated based on the endo-scopic activity index and the Mayo score. Results: Thirty of the 34 patients completed all 10 GMA treatment sessions. The overall efficacy rate was 70.59%. The receiver operating characteristic analysis showed that the area under the curve was approximately 0.766 for a Mayo score of ≤5.5 with 0.273 specificity and 0.857 sensitivity (Youden index, 0.584) for GMA responders. No GMA-related serious adverse events were observed. Conclusions: The overall efficacy of GMA in patients with active UC who were taking first-line medications or were corticosteroid refractory was encouraging. Addition-ally, GMA was well tolerated and had a good safety profile. (Gut Liver 2017;11:216-225)

A Review of Asia’s IPO Research

Yao-Min Chiang,Vivian Tai,Wanqin Zhang 한국재무학회 2021 財務硏究 Vol.34 No.1

A Conservative Formulation of the Apparent Heat Capacity Method for Heat Transport Problems with Phase Change

Min Wu Yao,Arnon Chait 대한기계학회 1993 International Symposium on Transport Phenomena in Vol.1 No.1

Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces

Yao-Dong Wu,Qi-Qi Wang,Meng Wang,Hany M. Elsheikha,Xin Yang,Min Hu,Xing-Quan Zhu,Min-Jun Xu 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.2

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

Design of ITLMS In-transit Logistics Monitoring System

Yao Zhong-min,Si Hong-yan,Lu Yanyang 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.12

A design of in-transit logistics remote video monitoring system is proposed based on cotex-A8 processor hardware and Android software systems platform, by the terminal USB infrared camera driver transplant, video streaming H.264 codec, and 3G wireless transmission network, the in-transit logistics remote video monitoring real-time display is achieved, which can prevent the loss of goods and cargo in logistics process and be served as the evidence for the responsibility distinguishing in the event of goods damage. Managers and customers can track the transport status of goods by computer or mobile phone in real-time, and provide better service to customer, therefore enhance the competitiveness of logistics enterprises.

DEVELOPMENT OF A THREE-DIMENSIONAL HIGH-ORDER ACCURATE DGM FLOW SOLVER ON UNSTRUCTURED MESHES

Min Yao,Oh Joon Kwon 한국전산유체공학회 2014 한국전산유체공학회 학술대회논문집 Vol.2014 No.5

A three-dimensional high-order flow solver based on discontinuous Galerkin method has been developed for the numerical simulations on unstructured meshes. The numerical test for the subsonic flow past a bump in a channel has been conducted to evaluate the efficiency and high-order accuracy of DGM flow solver. The subsonic flow past a NACA 0015 wing is also numerically simulated to demonstrate that DGM perfectly preserves the strength of wing tip vortex. Then, the flow around a Caradonna-Tung rotor in hover is simulated to validate the flow solver by means of comparing the computed pressure coefficients with experimental data. The numerical results demonstrate that the implicit discontinuous Galerkin methods with high-order representation of curved solid boundaries can achieve the designed high-order of accuracy.

Efficiently targeted therapy of glioblastoma xenograft via multifunctional biomimetic nanodrugs

Zhipeng Yao,Xiaochun Jiang,Hong Yao,Yafeng Wu,Fan Zhang,Cheng Wang,Chenxue Qi,Chenhui Zhao,Zeyu Wu,Min Qi,Jia Zhang,Xiaoxiang Cao,Zhichun Wang,Fei Wu,Chengyun Yao,Songqin Liu,Shizhang Ling,Hongping Xi 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Glioblastoma multiforme (GBM) is a fatal malignant primary brain tumor in adults. The therapeutic efficacy of chemotherapeutic drugs is limited due to the blood-brain barrier (BBB), poor drug targeting, and short biological half-lives. Multifunctional biomimetic nanodrugs have great potential to overcome these limitations of chemotherapeutic drugs. Methods: We synthesized and characterized a biomimetic nanodrug CMS/PEG-DOX-M. The CMS/PEG-DOX-M effectively and rapidly released DOX in U87 MG cells. Cell proliferation and apoptosis assays were examined by the MTT and TUNEL assays. The penetration of nanodrugs through the BBB and anti-tumor efficacy were investigated in the orthotopic glioblastoma xenograft models. Results: We showed that CMS/PEG-DOX-M inhibited cell proliferation of U87 MG cells and effectively induced cell apoptosis of U87 MG cells. Intracranial antitumor experiments showed that free DOX hardly penetrated the BBB, but CMS/PEG-DOX-M effectively reached the orthotopic ntracranial tumor through the BBB and significantly inhibited tumor growth. Immunofluorescence staining of orthotopic tumor tissue sections confirmed that nanodrugs promoted apoptosis of tumor cells. This study developed a multimodal nanodrug treatment system with the enhanced abilities of tumor-targeting, BBB penetration, and cancer-specific accumulation of chemotherapeutic drugs by combining chemotherapy and photothermal therapy. It can be used as a flexible and effective GBM treatment system and it may also be used for the treatment of other central nervous systems (CNS) tumors and extracranial tumors.

복합종균을 접종하여 발효한 메주의 특성

조민정 ( Min Jeong Cho ),심재민 ( Jae Min Shim ),이재용 ( Jae Yong Lee ),이강욱 ( Kang Wook Lee ),야오좡 ( Zhuang Yao ),류샤오밍 ( Xiaoming Liu ),김정환 ( Jeong Hwan Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.2

대두에 B. amyloliquefaciens 2 균주들과 P. farinose SY80, 그리고 R. oryzae 총 4 균주를 접종한 메주를 만들었다(메주 1). 대조구로 B. subtilis KACC16450과 A. oryzae를 접종한 메주(메주 2)와 볏짚을 균원시료로 접종한 메주(메주 3)들을 제조하여 총 56일 발효시켰다. 발효 기간중 메주 1과 2의 pH와 적정산도는 메주 3보다 각각 높고 그리고 낮았다. 메주의 혈전용해능은 메주 1이 가장 높았다. 발효 기간중 모든 메주에서 B. cereus는 검출되지 않았으나 메주 2와 3에서 여러 모양과 색을 띈 잡균들이 검출되었다. 메주 2에서 histamine이 그리고 메주 3에서 tyramine이 저 농도로 검출되었으나 메주 1에서는 둘 다 검출되지 않았다. B. amyloliquefaciens EMD17이나 MJ1-4와 같은 항균력 균주들을 접종하여 메주를 제조하면 미생물학적으로 보다 안전한 장류제품들 생산이 가능할 것이다. A test meju (meju 1) was prepared by inoculating two strains of Bacillus amyloliquefaciens (EMD17, MJ1-4), Pichia farinosa SY80, and Rhizopus oryzae into cooked soybeans. A control (meju 2) was prepared by inoculating Bacillus subtilis KACC16450 and Aspergillus oryzae. Another control (meju 3) was prepared using rice straw as the source of microorganisms. Three different mejus were fermented for 56 days outdoors. Meju 1 and meju 2 showed higher pH values than meju 3, whereas meju 3 showed higher titratable acidity than meju 1 and meju 2. Meju 1 showed the highest fibrinolytic activity. Bacillus cereus was not detected in any mejus, but various microorganisms were detected in meju 2 and 3. Histamine was detected in meju 2 and tyramine in meju 3 at lower concentration, which were not detected in meju 1. It was concluded that microbially safe, fermented soybean products could be produced from meju fermented with starters such as B. amyloliquefaciens EMD17 and B. amyloliquefaciens MJ1-4.

Properties of a Fibrinolytic Enzyme Secreted by Bacillus amyloliquefaciens RSB34, Isolated from Doenjang

( Zhuang Yao ),( Xiaoming Liu ),( Jae Min Shim ),( Kang Wook Lee ),( Hyun-jin Kim ),( Jeong Hwan Kim ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1

Nine bacilli with fibrinolytic activities were isolated from doenjang, a traditional Korean fermented soy food. Among them, RSB34 showed the strongest activity and was identified as Bacillus amyloliquefaciens by 16S rRNA and recA gene sequencing. During growth on LB up to 96 h, RSB34 showed the highest fibrinolytic activity (83.23 mU/μl) at 48 h. Three bands of 23, 27, and 42 kDa in size were observed when the culture supernatant was analyzed by SDSPAGE and 27 and 42 kDa bands by fibrin zymography. The gene encoding the 27 kDa fibrinolytic enzyme AprE34 was cloned by PCR. BLAST analyses confirmed that the gene was a homolog to genes encoding AprE-type proteases. aprE34 was overexpressed in Escherichia coli BL21(DE3) using pET26b(+). Recombinant AprE34 was purified and examined for its properties. The K_m and V_max values of recombinant AprE34 were 0.131 ± 0.026 mM and 16.551 ± 0.316 μM/l/min, respectively, when measured using an artificial substrate, Nsuccinyl- ala-ala-pro-phe-p-nitroanilide. aprE34 was overexpressed in B. subtilis WB600 using pHY300PLK. B. subtilis transformants harboring pHYRSB34 (pHY300PLK with aprE34) showed higher fibrinolytic activity than B. amyloliquefaciens RSB34.