In Vitro Evaluation and Monitoring of the Expression Level and Localization of Aldose Reductase Using Functionalized Quantum Dots and EGFP

Xiaomin Liu,Chengbin Yang,Jing Liu,Jianwei Liu,Rui Hu,Hongwei Lian,Guimiao Lin,Liwei Liu,Ken-Tye Yong,Ling Ye 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

The optimization of aldose reductase (AR) expression levels and tracking of the AR expression sites within the cell is an essential step in developing a platform for the effective production of aldose reductase inhibitors (ARIs). In this study, we have demonstrated the use of both immunocytochemistry and quantum dots-based immunofluorescence techniques for observing and detecting the expression level and localization of AR in the cytoplasm and cell membrane of a eukaryotic cell model with high levels of AR protein expression. Our results show that high expression levels of human AR can be achieved using the eukaryotic cell model that we have developed. The overexpressed AR can be used for translational studies of hAR and the screening of ARIs. More importantly, the use of the established quantum dots-based immunofluorescence technique in the intracellular labeling of AR allows the determination of the expression and distribution of the AR gene. Overall, the use of the interdisciplinary approach of both genetic engineering and quantum dot-based immunofluorescence allows not only the effective production of a desired protein, but also the determination of the cellular localization of such an expressed protein.

Observer-based Actuator Fault Detection and Robust Tolerant Control for Vehicle Platoons

Xiaomin Liu,Maode Yan,Panpan Yang,Jiacheng Song 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.4

This paper investigates the actuator fault detection and tolerant control problem for vehicle platoons with partial loss of effectiveness and bias faults. Considering the inherent coupling between the control input and the nonlinear term in the actuator, a decoupled observer based fault detection algorithm with time-varying threshold is developed to decide whether the actuator fault occurs in the platoon. Then, by employing radial basis function neural networks (RBF NNs) and backstepping control method, an adaptive robust fault-tolerant control (FTC) scheme is proposed, which is proved to be capable of achieving the safety, stability and tracking performance of vehicle platoons in the presence of actuator fault. Simulation studies illustrate the effectiveness of the proposed method.

Nitrogen removal and metabolic pathways of nitrifying coal ash propelled by temperature change under high nitrogen loading

Fang Liu,Xin Zhao,Chengjian Liu,Xiaomin Hu 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.5

Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy

Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5

Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.

Cellular and Molecular Mechanisms of Intestinal Fibrosis

Wu Xiaomin,Lin Xiaoxuan,Tan Jinyu,Liu Zishan,He Jinshen,Hu Fan,Wang Yu,Chen Minhu,Liu Fen,Mao Ren 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.3

Intestinal fibrosis associated stricture is a common complication of inflammatory bowel disease usually requiring endoscopic or surgical intervention. Effective anti-fibrotic agents aiming to control or reverse intestinal fibrosis are still unavailable. Thus, clarifying the mechanism underpinning intestinal fibrosis is imperative. Fibrosis is characterized by an excessive accumulation of extracellular matrix (ECM) proteins at the injured sites. Multiple cellular types are implicated in fibrosis development. Among these cells, mesenchymal cells are major compartments that are activated and then enhance the production of ECM. Additionally, immune cells contribute to the persistent activation of mesenchymal cells and perpetuation of inflammation. Molecules are messengers of crosstalk between these cellular compartments. Although inflammation is necessary for fibrosis development, purely controlling intestinal inflammation cannot halt the development of fibrosis, suggesting that chronic inflammation is not the unique contributor to fibrogenesis. Several inflammation-independent mechanisms including gut microbiota, creeping fat, ECM interaction, and metabolic reprogramming are involved in the pathogenesis of fibrosis. In the past decades, substantial progress has been made in elucidating the cellular and molecular mechanisms of intestinal fibrosis. Here, we summarized new discoveries and advances of cellular components and major molecular mediators that are associated with intestinal fibrosis, aiming to provide a basis for exploring effective anti-fibrotic therapies in this field.

The clinical outcomes of fresh versus frozen embryos transfer in women ≥40 years with poor ovarian response

Conghui Liu,Yu Li,Hong Jiang,Yingchun Liu,Xiaomin Song 대한산부인과학회 2021 Obstetrics & Gynecology Science Vol.64 No.3

ObjectiveTo compare the clinical outcomes of fresh embryo transfers (ETs) and frozen-thawed embryo transfers (FETs) after afreeze-all cycle in women ≥40 years old with poor ovarian response (POR). MethodsWe performed a single-center, retrospective, case-control study of patients who underwent in vitro fertilizationbetween January 2014 and June 2019. We included a total of 192 patients aged 40 years or older from whom 3 orfewer oocytes had been retrieved and who were receiving cleavage-stage ET in this study. Of these patients, 101 and91 patients underwent fresh ET and FET, respectively. The primary outcome was the live birth rate (LBR) after thefirst ET. Logistic regression analysis was used to compare the IVF outcomes and neonatal characteristics between thefresh ET and FET groups, adjusting for maternal age, body mass index, luteinizing hormone, and the number of goodquality embryos transferred. ResultsThe mean maternal ages and number of oocytes retrieved (43.2 years and 2.3 in both groups, P=0.902 and P=0.927,respectively) were similar in the fresh ET and FET groups. No significant difference was observed between the LBRsof the fresh ET and FET groups (adjusted odds ratio, 1.28; 95% confidence interval, 0.29–5.70). The clinical pregnancyand miscarriage rates, and neonatal characteristics (birth weights and premature infant rates) were similar betweenthe 2 groups. ConclusionsFET after the freeze-all strategy had no beneficial impact on the clinical outcomes of women ≥40 years with POR.

Analysis and research on dynamic characteristics of 3D braided composite two-stage reducer case

Weiliang Zhang,Xupeng Wang,Xiaomin Ji,Xinyao Tang,Fengfeng Liu,Shuwei Liu,Mingjun Liu,Xiao-Long Gao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.6

Based on the analysis of the movement rule of 3D five-way braided yarn, the 3D five-way braided case model is divided into three parts: base, case bottom plate and case wall. According to the characteristics of each part, the braiding and forming principle of a 3D five-way composite case was analyzed. Under the assumption of ignoring the influence of friction, bearing and other factors on the system, the differential equations of motion of the two-stage gear transmission system with case were established. The two-stage gear transmission system was numerically analyzed by Euler method, and the influence of dimensionless meshing frequency, clearance, case mass and damping on the dynamic characteristics of the transmission system was studied. After that, high and low frequency disturbance was applied to the input case. The research shows that the damping ratio of the composite case increases and the low frequency attenuation is accelerated due to the decrease of the case mass, so the composite case has better shock resistance to low frequency interference.

Thin-film electronics based on all-2D van der Waals heterostructures

Liu Xinling,Yang Xiaomin,Sang Weihui,Huang Hai,Li Wenwu,Lin Yen-Fu,Chu Junhao 한국정보디스플레이학회 2021 Journal of information display Vol.22 No.4

Two-dimensional (2D) layered materials including metal, semiconductor, and insulator have received extensive attention in recent years. The weak van-der-Waals (vdW) interactions between 2D materials layers enable them to isolate monolayers and restack into artificial 2D vdW heterostructures in the desired sequence. These assembled all-2D vdW heterostructures are promising platforms for fabricating next-generation electronics as well as optoelectronics. In particular, the all-2D vdW heterostructure devices composed entirely of 2D layered material have received extensive attention due to their natural thickness, atomically sharp heterointerfaces, and excellent mechanical flexibility. Herein, we firstly introduce 2D vdW heterostructures and their preparation methods. Secondly, the recent progress of field-effect transistors (FETs) and photodetectors based on all-2D vdW heterostructures are summarized. Finally, we discuss some challenges of all-2D vdW heterostructure-based devices for practical applications and offer personal perspectives toward the future development of thin-film electronics.

Preparation and Characterization of Regenerated Cellulose Blend Films Containing High Amount of Poly(vinyl alcohol) (PVA) in Ionic Liquid

Xiaomin Zhang,Jin Zhu,Xiaoqing Liu 한국고분자학회 2012 Macromolecular Research Vol.20 No.7

Regenerated cellulose/poly(vinyl alcohol) (PVA) blend films in ionic liquid 1-allyl-3-methylimidazolium chloride (AMIMCl) were successfully prepared by coagulating with water. The structures and properties were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and differential scanning calorimetry (DSC). SEM images indicated that there was good compatibility between cellulose and PVA. The XRD results showed that the crystallinity of cellulose was significantly decreased from 42.8% up to 29.4% with increasing PVA content. The blend film with a 10 wt% PVA demonstrated the best mechanical performance. Its tensile strength and modulus showed 97 and 5,100 MPa, respectively compared with 69 and 3,400 MPa of neat cellulose film. The elongation also increased 50%. In addition, the transparency of the blend lms was still maintained well, and the shrinkage of the regenerated cellulose film was greatly improved with incorporation of PVA. The possible mechanism of forming cellulose/PVA blend films was proposed in the process of regeneration and dissolution.

A Throughput Improved Path Selection Method Based on Throughput Prediction Model and Available Bandwidth for MPTCP

Xiaomin Jin,Yuanan Liu,Wenhao Fan,Fan Wu,Hongguang Zhang 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.2

Nowadays, many network devices have more than one network interface. Meanwhile, many protocols have been proposed to utilize multiple paths simultaneously. MPTCP (Multipath TCP) is a transport layer protocol that transfers data by multiple paths simultaneously, which is designed to improve the throughput and increase robustness of end to end transmission. However, when we use MPTCP, we find that MPTCP can not improve the throughput but even reduce the throughput in some cases. In this paper, we study this issue. Firstly, we study the effects of path characteristics for throughput of MPTCP, and find that path characteristics especially those bad paths have a great impact on the performance of MPTCP. Secondly, we analyze the reasons why the bad path characteristics have a serious impact on throughput of MPTCP, and propose a path selection method based on throughput prediction model and available bandwidth to improve the throughput. Finally, we validate our path selection method by simulations, and the results show that our method can improve the throughput of MPTCP.