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Liu, Xiaoqing,Yuan, Lingling,Xu, Ling,Xu, Zhaolong,Huang, Yihong,He, Xiaolan,Ma, Hongxiang,Yi, Jinxin,Zhang, Dayong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4
In this paper, we isolated and characterized a gene encoding the soybean MYB transcription factor, GmMYB39 (Accession No: XM_003538605). Analysis of the deduced amino acid sequence revealed that GmMYB39 contained N-terminal R2R3 repeats that corresponded to the DNA-binding domain of plant MYB-type proteins, which were highly conserved among the R2R3-MYB proteins. The detailed expression pattern of GmMYB39 in various tissues of soybean was investigated by quantitative RT-PCR. The transcript level was found to be higher in flowers than in other examined organs. In contrast, the GmMYB39 expression level was relatively weak in the pod. The GmMYB39-GFP fusion protein was found to localize in the nucleus of Arabidopsis mesophyll protoplasts. Over-expression of GmMYB39 in hairy roots resulted in a significant reduction of the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Whereas, the IFS transcript level was slightly but not significantly increased, and no significant change in CHI expression was observed between over-expression and control roots. The repressing effect on expression of CHS was further supported by the results from co-transfection assays of CHS promoter (reporter) and GmMYB39 (effector) in soybean. Compared to the control (only with the reporter construct), a significant decrease in the GUS activity was observed in soybean hairy roots with both effector and reporter constructs. Furthermore, an ultimate decrease in isoflavonoids contents coincided with the decrease in the transcript levels of PAL, C4H, CHS, 4CL, and CHR. Overall, the results suggest that GmMYB39 plays an inhibiting role in regulating the isoflavonoid biosynthesis in soybean.
Liu Genshen,Wei Peitang,Liu Huaiju,Du Xuesong,Hu Rui,He Huilin,Zhou Pengliang,Tan Xiaoqing 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.6
The planetary roller screw mechanism (PRSM) is an advanced linear transmission device. The relationship between tolerance allocation and performance risk still remains elusive, which is a challenge for its future applications. This work proposes a novel transmission accuracy–axial backlash–fatigue life-driven tolerance optimization method for the screw, roller, and nut of PRSM. A computational framework for PRSM transmission accuracy, axial backlash, and fatigue life calculation is developed to work on the parametric variation of design parameters including the eccentric, pitch, nominal diameter, and flank angle. Combinations of parametric variation are obtained by the Latin hypercube sampling-based tolerance statistical model to rapidly evaluate PRSM performance risk under operation conditions and tolerance parameters. The optimal tolerance parameters with the expanded width of tolerance interval and the minimum PRSM performance risk probability are obtained using the non-dominated sorting genetic algorithm. Results reveal that PRSM performance risk probabilities change from 89.25 to 68.72% and 58.1 to 56.86%, with 29.94 and 17.38% tolerance interval width increase under the high-precision and heavy-loading operation cases studied, respectively.
LIU Xiaoqing 이화여자대학교 아시아여성학센터 2011 Asian Journal of Women's Studies(AJWS) Vol.17 No.4
Sophia and Coco are two characters in Ding Ling's Miss Sophia's Diary (1928/1995) and Wei Hui's Shanghai Baby (1999), respectively. Like a larva, Sophia, who enters society in the early twentieth century, is weak and immobile. Coco, who lives at the end of the twentieth century, is like a butterfly leading an outlandish lifestyle. The differences between Sophia and Coco reflect the achievement of official feminism, which liberated Chinese women from traditional patriarchal control in the social sense. However, gender issues for women remain unresolved. To fight against traditional patriarchy and especially challenge gender oppression in official feminism, both Sophia and Coco boldly acknowledge and pursue sexual desire. Their feminist discourses are both commendable and controversial, given their situations and the times in which they live.
A Novel Video Stitching Method for Multi-Camera Surveillance Systems
( Xiaoqing Yin ),( Weili Li ),( Bin Wang ),( Yu Liu ),( Maojun Zhang ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.10
This paper proposes a novel video stitching method that improves real-time performance and visual quality of a multi-camera video surveillance system. A two-stage seam searching algorithm based on enhanced dynamic programming is proposed. It can obtain satisfactory result and achieve better real-time performance than traditional seam-searching methods. The experiments show that the computing time is reduced by 66.4% using the proposed algorithm compared with enhanced dynamic programming, while the seam-searching accuracy is maintained. A real-time local update scheme reduces the deformation effect caused by moving objects passing through the seam, and a seam-based local color transfer model is constructed and applied to achieve smooth transition in the overlapped area, and overcome the traditional pixel blending methods. The effectiveness of the proposed method is proved in the experiements.
Evaluation of Fungal Lactic Acid Accumulation Using Glycerol as the Sole Carbon Source
Xiaoqing Wang,Zhenhua Ruan,Webster Guan,Robert Kraemer,Yuan Zhong,Yan Liu 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
This study investigated the glycerol utilization for lactic acid accumulation by the filamentous fungus Rhizopus oryzae 9363 and compared it with the conventional carbon source glucose. No lactic acid accumulated in the glycerol media at 30oC, in contrast to the glucose media. Increasing the temperature from 30 to 37oC led to a 63% decrease in the average growth rate of R. oryzae in glycerol media and a 61% increase in the average cell mass yield, and the cultures on glycerol media at 37oC were able to generate 0.6 g/L lactic acid. While, raising temperature significantly inhibited lactic acid production on glucose media. Moreover, supplementing cultures with sodium pyruvate significantly improved the lactic acid synthesis of R. oryzae on glycerol media, with lactic acid concentrations reaching 1.33 g/L at 37oC and 0.67 g/L at 30oC, respectively. Our results indicate that glycerol utilization for lactic acid accumulation by Rhizopus sp. is limited by the availability of intracellular pyruvate, and controlling pyruvate flow is a key to enhancing the lactic acid accumulation.
( Xiaoqing Yin ),( Bin Wang ),( Weili Li ),( Yu Liu ),( Maojun Zhang ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.10
We propose a background subtraction method for moving cameras based on trajectory classification, image segmentation and label inference. In the trajectory classification process, PCA-based outlier detection strategy is used to remove the outliers in the foreground trajectories. Combining optical flow trajectory with watershed algorithm, we propose a trajectory-controlled watershed segmentation algorithm which effectively improves the edge-preserving performance and prevents the over-smooth problem. Finally, label inference based on Markov Random field is conducted for labeling the unlabeled pixels. Experimental results on the motionseg database demonstrate the promising performance of the proposed approach compared with other competing methods.
Xiaoqing Lin,Renjie Li,Qingshi Wen,Jinglan Wu,Jiansheng Fan,Xiaohong Jin,Wenbin Qian,Dong Liu,Xiaochun Chen,Yong Chen,Jingjing Xie,Jianxin Bai,Han-Jie Ying 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2
Removal of biobutanol from acetone-butanolethanol (ABE) fermentation broth can be achieved by fixed-bed sorption by means of KA-I resin, and the relevant breakthrough curves would provide much valuable information to help design a continuous fixed-bed sorption process in field application. In the present study, the effects of several important design parameters, i.e., initial butanol concentration (Cf: 3.0 ~ 30.0 g/L), inlet flow rate (Qf: 0.5~ 5.5 mL/min) and adsorbent bed height (Z: 4.2 ~ 18.0 cm),on the adsorption breakthrough curves of KA-I resin in a fixed-bed column were investigated. It was found that the amount of adsorbed butanol at breakthrough point was increased with an increase in the value of Cf and Z; and with decrease in the value of Qf. However, the maximum sorption capacities of butanol at saturated point were basically unchanged. Three well-established fixed-bed adsorption models, namely Thomas, Yoon-Nelson and Adams-Bohart,were applied to predict the breakthrough curves and to determine the characteristic parameters of fixed-bed column,which are the basis for the process design at a real scale. Good agreement between the theoretical breakthrough curves and the experimental result were observed using Thomas and Yoon-Nelson models.
Digital Watermark of 3D CAD Product Model
Xiaoqing Feng,Yanan Liu,Lincong Fang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.9
This paper proposes a novel watermarking algorithm of 3D CAD product model, which is able to keep strictly geometrical shape around embedding watermark and make watermarked model have function transparency. Firstly, the isolated geometrical entities are selected as watermark carriers. Secondly, the watermark is embedded into the isolated geometrical entities with a geometric entity substitution algorithm. When extracting the watermark, the original product model and pre-process (registration or re-sampling) need not be provided. The experimental results have shown that the proposed watermarking algorithm is robustness against some common attacks, such as transformation, rotation, scaling and entity deletion.