Genome and transcriptome sequencing of a newly isolated 2,4-dinitrophenol-degrading strain Rhodococcus imtechensis XM24D

Fan Hu,Longhe Yang,Zhaokai Wang,Jinpei Wang 한국유전학회 2021 Genes & Genomics Vol.43 No.7

Background 2,4-Dinitrophenol (2,4-DNP) is an important organic environmental pollutant that is highly toxic to all forms of living organisms. A gram-positive strain (designated XM24D) was isolated from 2,4-DNP-contaminated soil by an enrichment technique. Objective The study was designed to analyze the ability of XM24D to degrade 2,4-DNP and its analogs and to reveal the degradation pathways of these aromatic compounds. Methods The degradation ability of XM24D was tested by a growth experiment. 2,4-DNP and its analog degradation pathways were predicted by genome and comparative transcriptome sequencing. Results Growth profles showed that XM24D was able to utilize 2,4-DNP as the sole source of carbon, nitrogen and energy. Analogs of 2,4-DNP, including 4-nitrophenol (PNP) and 2-chloro-4-nitrophenol (2C4NP), can also be degraded by XM24D. Genome analysis showed that the XM24D genome contains two chromosomes with a combined size of 9.08 Mb and an average GC content of 67.07%. Average nucleotide identity analysis indicated that Rhodococcus imtechensis RKJ300 is the most closely related strain to XM24D. Comparative transcriptome analysis revealed that the 2,4-DNP/PNP/2C4NP degradation pathway in XM24D is highly similar in sequence and organization to the 2,4-DNP degradation pathway in Rhodococcus opacus HL PM-1, the PNP degradation pathway in Rhodococcus opacus SAO101 and the 2C4NP degradation pathway in Rhodococcus imtechensis RKJ300. These results suggested that 2,4-DNP/PNP/2C4NP was degraded via the 2,4-dinitrocyclohexanone/4-nitrocatechol/hydroxyquinol pathway in XM24D. Conclusions Genomic and transcriptomic information on XM24D provides a valuable reference for further investigating the evolutionary characteristics of nitrophenol degradation pathways in microorganisms.

Multilingual Computer Processing System

Hu Fan Jin,Ru Lin Liu,Ri Di Ga,Zhung Rao Wu 대한전자공학회 1991 International Conference on Electronics, Informati Vol.1 No.1

Optimal design of bio-inspired isolation systems using performance and fragility objectives

Hu, Fan,Shi, Zhiguo,Shan, Jiazeng Techno-Press 2018 Structural monitoring and maintenance Vol.5 No.3

This study aims to propose a performance-based design method of a novel passive base isolation system, BIO isolation system, which is inspired by an energy dissipation mechanism called 'sacrificial bonds and hidden length'. Fragility functions utilized in this study are derived, indicating the probability that a component, element, or system will be damaged as a function of a single predictive demand parameter. Based on PEER framework methodology for Performance-Based Earthquake Engineering (PBEE), a systematic design procedure using performance and fragility objectives is presented. Base displacement, superstructure absolute acceleration and story drift ratio are selected as engineering demand parameters. The new design method is then performed on a general two degree-of-freedom (2DOF) structure model and the optimal design under different seismic intensities is obtained through numerical analysis. Seismic performances of the biologically inspired (BIO) isolation system are compared with that of the linear isolation system. To further demonstrate the feasibility and effectiveness of this method, the BIO isolation system of a 4-storey reinforced concrete building is designed and investigated. The newly designed BIO isolators effectively decrease the superstructure responses and base displacement under selected earthquake excitations, showing good seismic performance.

Attributable Causes of Liver Cancer Mortality and Incidence in China

Fan, Jin-Hu,Wang, Jian-Bing,Jiang, Yong,Xiang, Wang,Liang, Hao,Wei, Wen-Qiang,Qiao, You-Lin,Boffetta, Paolo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Objectives: To estimate the proportion of liver cancer cases and deaths due to infection with hepatitis B virus (HBV), hepatitis C virus (HCV), aflatoxin exposure, alcohol drinking and smoking in China in 2005. Study design: Systemic assessment of the burden of five modifiable risk factors on the occurrence of liver cancer in China using the population attributable fraction. Methods: We estimated the population attributable fraction of liver cancer caused by five modifiable risk factors using the prevalence data around 1990 and data on relative risks from meta-analyses, and large-scale observational studies. Liver cancer mortality data were from the 3rd National Death Causes Survey, and data on liver cancer incidence were estimated from the mortality data from cancer registries in China and a mortality/incidence ratio calculated. Results: We estimated that HBV infection was responsible for 65.9% of liver cancer deaths in men and 58.4% in women, while HCV was responsible for 27.3% and 28.6% respectively. The fraction of liver cancer deaths attributable to aflatoxin was estimated to be 25.0% for both men and women. Alcohol drinking was responsible for 23.4% of liver cancer deaths in men and 2.2% in women. Smoking was responsible for 18.7% and 1.0%. Overall, 86% of liver cancer mortality and incidence (88% in men and 78% in women) was attributable to these five modifiable risk factors. Conclusions: HBV, HCV, aflatoxin, alcohol drinking and tobacco smoking were responsible for 86% of liver cancer mortality and incidence in China in 2005. Our findings provide useful data for developing guidelines for liver cancer prevention and control in China and other developing countries.

An integrated dendrite-free zinc metal electrode for corrosion inhibition in aqueous system

Yi-Fan Hu,Li-Feng Zhou,He Gong,He Jia,Peng Chen,Yi-Song Wang,Li-Ying Liu,Tao Du 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.9

Zinc ion batteries have gotten increasing attention as a potential candidate for lithium-ion batteries, due totheir high specific capacity (820 mAh·g1), energy density, and safety. Inevitably, dendrite and corrosion create sometrouble for this system. Herein, an integrated Zn electrode coated by Zn-Al metal oxides prepared by a simple spincoatingmethod was utilized to increase the rechargeability for aqueous zinc ion batteries. By coating the Zn anodewith an artificial electrolyte interface, the wettability of Zn anodes was improved and impedance was reduced. Thecoating suppressed not only the appearance of dendrite but also the formation of corrosion products. The symmetricalcells with coating have a low overpotential (43mV) and an excellent life span. Meanwhile, the applied full batteriesexhibit an improved capacity retention rate (86.67% after 120 cycles), great rate performance and low apparent activationenergy (24.6 KJ·mol1). The simple production methods and superior corrosion suppression effects provide newideas for the anode protection of aqueous system batteries.

Research of Power Battery Management System in Electric Vehicle

Yinquan Hu,Xiaobing Wu,Jianshan Tu,Qiheng Fan 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2

Power battery management system is a very important part in electric vehicle. Currently, power battery management system is only monitor and control of the power batteries group voltage. This may cause a single battery over-charge and over-discharge, thus affecting cycle life of the power battery. In this paper, for each cell in the batteries group is monitored and controlled to prevent any battery over-charge and over-discharge. Hardware and software of power battery management system has been designed. Experimental results show that power battery management system can accurately monitor the power batteries, thereby ensuring the safety of the power batteries and prolonging the cycle life of the power batteries group.

Development of SSR molecular markers for Allium mongolicum

Jing Hu,Xiaoke Hu,Qian Zhang,Jinhu Zhang,Baoli Fan,Qiushi Yu 한국유전학회 2017 Genes & Genomics Vol.39 No.12

Allium mongolicum is high palatability, nutrient, medicinal value and drought resistance wild plant. However, a lack of molecular markers of this plant results in a series of genetic questions remain largely unknown, including molecular marker data, population genetic structure and accuracy of genetic breeding. In this study, a total of 1,409,706 quality- filtered and trimmed reads were obtained from the normalized genomic DNA of pooled A. mongolicum individuals. These sequences were assembled into 2,093,593 high quality contigs, and a total of 219 simple sequence repeats loci were screened. Sixty of them were selected to validate amplification and to determine the degree of polymorphism in the genomic DNA pools. Fifteen primer pairs successfully amplified DNA fragments and detected significant amounts of polymorphism. The number of alleles in ten geographically diverse A. mongolicum populations ranged from 3 to 8 per locus. The observed and expected heterozygosities ranged from 0.350 to 0.860 and 0.516 to 0.931, respectively. Our results provide a valuable resource for A. mongolicum research.

서비스 실패의 심각성과 통제성이 회복만족에 미치는 영향

유호범(Hu, Fan-Yu),지희진(Ji, Hee-Jin),강기두(Kang, Gi-Du) 대한경영학회 2013 大韓經營學會誌 Vol.26 No.4

SEDs and Beaming Effect for Fermi Blazars

Jun-Hui Fan,Jiang-He Yang,Yi Liu,Yu-Hai Yuan,Cao Lin,Hu-Bing Xiao 한국우주과학회 2016 Journal of Astronomy and Space Sciences Vol.33 No.2

In this work, based on our previous calculations of spectral energy distributions for a sample of Fermi blazars (Fan et al. 2015a), we calculated the radio loudness and performed correlation analyses. Our analysis results show that radio loudness is closely anti-correlated with synchrotron peak frequency and positively correlated with gamma-ray luminosity, suggesting that the gamma-ray emissions are strongly beamed.

Experimental Study on the Corrosion of Buried Pipeline in Coastal Area Under the Interference of Stray Current

Fan Xu,Huijun Wu,Xin Hu,Zhong-Chang Wang 한국전기전자재료학회 2019 Transactions on Electrical and Electronic Material Vol.20 No.5

To explore the eff ect of stray current and the concentration of Cl − on corrosion of buried pipeline in coastal area, in this paper, indoor electrochemical experiments were used to change the voltage of AC interference. The electrochemical parameters such as zero current potential error, Tafel constant and corrosion current density error of the polarization curves obtained in three diff erent concentrations of Cl − solutions without alternating current interference and with diff erent intensities of alternating current interference were compared and measured. The results showed that the corrosion potential of the sample was shifted negatively. When the interference ratio of the fi xed voltage was not disturbed, the corrosion current increased, and the corrosion process changed from cathode reaction control to hybrid control. AC stray current could aggravate the corrosion process of Q235 steel. In the presence of AC stray current, with the increase of applied AC voltage, the corrosion potential of Q235 steel moved negatively, the corrosion current density increased and the corrosion rate accelerated. The concentration of Cl − had a great infl uence on the reaction rate, and the higher the voltage, the more obvious the eff ect. This is of great signifi cance for eff ectively predicting the life of buried pipelines, improving the reliability of buried pipelines during service and protecting the safety of existing pipeline facilities.