In Vitro Evaluation and Monitoring of the Expression Level and Localization of Aldose Reductase Using Functionalized Quantum Dots and EGFP

Xiaomin Liu,Chengbin Yang,Jing Liu,Jianwei Liu,Rui Hu,Hongwei Lian,Guimiao Lin,Liwei Liu,Ken-Tye Yong,Ling Ye 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

The optimization of aldose reductase (AR) expression levels and tracking of the AR expression sites within the cell is an essential step in developing a platform for the effective production of aldose reductase inhibitors (ARIs). In this study, we have demonstrated the use of both immunocytochemistry and quantum dots-based immunofluorescence techniques for observing and detecting the expression level and localization of AR in the cytoplasm and cell membrane of a eukaryotic cell model with high levels of AR protein expression. Our results show that high expression levels of human AR can be achieved using the eukaryotic cell model that we have developed. The overexpressed AR can be used for translational studies of hAR and the screening of ARIs. More importantly, the use of the established quantum dots-based immunofluorescence technique in the intracellular labeling of AR allows the determination of the expression and distribution of the AR gene. Overall, the use of the interdisciplinary approach of both genetic engineering and quantum dot-based immunofluorescence allows not only the effective production of a desired protein, but also the determination of the cellular localization of such an expressed protein.

FEASIBILITY ANALYSIS OF CHANGING WATER SOURCE SYSTEM OF MOON-LAKE RESERVOIR

LIU JIANWEI,XU SHIGUO,LIU YINGFEI 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Dynamic Analysis of Slab Track with Crack Considering Rail Corrugation

Jianwei Yang,Peishan Liu,Xiaohui Wang,Yue Zhao,Fu Liu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.12

To investigate the dynamic response of ballastless slab track with crack in the rail corrugation area under the load of urban rail vehicle, this study establishes a vertical coupling dynamic model of the vehicle-rail-slab track, considering crack and rail corrugation. The exact closed-form solution of the vibration mode of the Euler Bernoulli beam with crack is introduced. Furthermore, a finite element model of the slab track is developed, and the vertical dynamic model's fastening force is applied to the slab track at the rail corrugation frequency to calculate the surface compressive stress of the slab track. The results indicate that, under rail corrugation conditions of varying magnitudes, the presence of crack induces higher vertical acceleration amplitudes in the slab track at low frequencies. As the wave depth increases, the acceleration of the slab track at the crack gradually intensifies. Moreover, with an increasing wavelength, the corresponding frequency to the acceleration amplitude of the slab track at the crack gradually decreases. Notably, when the wave depth is 0.2 mm and the wavelength is 50 mm, the axle load transfer rate exhibits the greatest increase. These research findings offer valuable insights for the maintenance of urban rail transit slab track systems.

Compound control strategy for maximum power point tracking with flexible step-up converters for thin film photovoltaic module applications

Liu, Yuhang,Liu, Xiangxin,Zhang, Jianwei,Zhang, Yufeng,Liu, Guangchen The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.9

This paper proposes a novel maximum power point tracking (MPPT) algorithm for a thin-film photovoltaic (PV) module with a flexible step-up DC-DC converter. To improve the voltage rating for the thin film module, a switch-inductor zero voltage transition (SIZVT) boost converter is proposed. In addition, the proposed methodology uses a multistage variable step size (MVSS) with a power close-loop control strategy (PCLC) for MPPT. The corresponding performances are compared with the classical fixed step size (FSS) algorithm and the conventional variable step size (CVSS) algorithm. Simulation results in MATLAB/Simulink and experimental tests with a 240 W prototype validate the proposed algorithm under a variety of conditions.

A 4x Time-Domain Interpolation 6-bit 3.4 GS/s 12.6 mW Flash ADC in 65 nm CMOS

Liu, Jianwei,Chan, Chi-Hang,Sin, Sai-Weng,U, Seng-Pan,Martins, Rui Paulo The Institute of Electronics and Information Engin 2016 Journal of semiconductor technology and science Vol.16 No.4

A 6-bit 3.4 GS/s flash ADC in a 65 nm CMOS process is reported along with the proposed 4x time-domain interpolation technique which allows the reduction of the number of comparators from the conventional $2^N-1$ to $2^{N-2}$ in a N-bit flash ADC. The proposed scheme effectively achieves a 4x interpolation factor with simple SR-latches without extra clocking and calibration hardware overhead in the interpolated stage where only offset between the $2^{N-2}$ comparators needs to be calibrated. The offset in SR-latches is within ${\pm}0.5$ LSB in the reported ADC under a wide range of process, voltage supply, and temperature (PVT). The design considerations of the proposed technique are detailed in this paper. The prototype achieves 3.4 GS/s with 5.4-bit ENOB at Nyquist and consumes 12.6 mW power at 1 V supply, yielding a Walden FoM of 89 fJ/conversion-step.

A 4x Time-Domain Interpolation 6-bit 3.4 GS/s 12.6 ㎽ Flash ADC in 65 ㎚ CMOS

Jianwei Liu,Chi-Hang Chan,Sai-Weng Sin,Seng-Pan U,Rui Paulo Martins 대한전자공학회 2016 Journal of semiconductor technology and science Vol.16 No.4

A 6-bit 3.4 GS/s flash ADC in a 65 ㎚ CMOS process is reported along with the proposed 4x time-domain interpolation technique which allows the reduction of the number of comparators from the conventional 2<SUP>N</SUP>-1 to 2<SUP>N-2</SUP> in a N-bit flash ADC. The proposed scheme effectively achieves a 4x interpolation factor with simple SR-latches without extra clocking and calibration hardware overhead in the interpolated stage where only offset between the 2<SUP>N-2</SUP> comparators needs to be calibrated. The offset in SR-latches is within ±0.5 LSB in the reported ADC under a wide range of process, voltage supply, and temperature (PVT). The design considerations of the proposed technique are detailed in this paper. The prototype achieves 3.4 GS/s with 5.4-bit ENOB at Nyquist and consumes 12.6 ㎽ power at 1 V supply, yielding a Walden FoM of 89 fJ/conversion-step.

Effect on tower crane structural vibration under the lifting-luffing coupling condition

Fu Liu,Jianwei Yang,Jinhai Wang,Changdong Liu,Xiaohui Wang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.10

The present research aims to investigate parametrically the structural vibration analysis of tower crane under the lifting-luffing coupling condition. Structural vibration greatly affects tower crane stability. The structural dynamics of a crane should be considered for studying motion behavior and vibration feature. The dynamic model of the tower crane is presented based on air resistance, boom deflection, and Lagrange equation under the lifting-luffing coupling condition. The vibration model of the tower crane was validated by an experiment. The experiment was conducted with an accelerating speed until reaching the constant speed and then with decelerating speed. This study analyses structural parameters to acquire the dynamic characteristics and vibration features of the tower crane under the compound working condition. The vibration responses of the mast and the boom are studied with frequency- and timedomain indicators. Results show that payload mass, luffing acceleration, boom length, mast height, and girder length significantly influence the mast vibration. In comparison, payload mass, trolley acceleration, and boom length affect the boom vibration. This research can improve the design of a dynamic system for tower crane safety.

Autophagy of bovine mammary epithelial cell induced by intracellular Staphylococcus aureus

Na Geng,Kangping Liu,Jianwei Lu,Yuliang Xu,Xiaozhou Wang,Run Wang,Jianzhu Liu,Yongxia Liu,Bo Han 한국미생물학회 2020 The journal of microbiology Vol.58 No.4

Bovine mastitis is a common disease in the dairy industry that causes great economic losses. As the primary pathogen of contagious mastitis, Staphylococcus aureus (S. aureus) can invade bovine mammary epithelial cells, thus evading immune defenses and resulting in persistent infection. Recently, autophagy has been considered an important mechanism for host cells to clear intracellular pathogens. In the current study, autophagy caused by S. aureus was detected, and the correlation between autophagy and intracellular S. aureus survival was assessed. First, a model of intracellular S. aureus infection was established. Then, the autophagy of MAC-T cells was evaluated by confocal microscopy and western blot. Moreover, the activation of the PI3K-Akt-mTOR and ERK1/2 signaling pathways was determined by western blot. Finally, the relationship between intracellular bacteria and autophagy was analyzed by using autophagy regulators (3-methyladenine [3-MA], rapamycin [Rapa] and chloroquine [CQ]). The results showed that S. aureus caused obvious induction of autophagosome formation, transformation of LC3I/II, and degradation of p62/SQSTM1 in MAC-T cells; furthermore, the PI3K-Akt-mTOR and ERK1/2 signaling pathways were activated. The number of intracellular S. aureus increased significantly with autophagy activation by rapamycin, whereas the number decreased when the autophagy flux was inhibited by chloroquine. Therefore, this study indicated that intracellular S. aureus can induce autophagy and utilize it to survive in bovine mammary epithelial cells.

Analysis on Slope Stability of Open Pit Coal Mine Based on Grey Support Vector Machine

Liu Bo,Zhang Peng,Zhang Jianwei 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.9

Shenhua BaoRi XiLe open-pit coal mine slope is located in the seismic belt of hulunbuir prairie in Inner Mongolia, which belongs to the high frequency and intensity earthquake zone, with rainfall concentrated, poor geological distribution, under the influence of complex environmental factors, extremely easy to cause landslide. This paper uses grey model and support vector machine (SVM) to establish slope model, and carries out stress and strain characteristics under the condition of natural, rainfall, earthquake and calculation and analysis of stability. Through the experiment, it analyzes the stability coefficient of downhill under rainfall condition, and obtains the different strength reduction of safety factor list. At the same time, give full consideration to the uncertainty of the earthquake, assume a variety of working conditions of the earthquake, it conducts dynamic analysis of the slope in detail, and gets the dynamic characteristics curves of the monitoring points. Results show that, relative complex terrain conditions, climate conditions and at a high intensity earthquakes area, are the main factors to induce landslides in this region. Therefore, this paper provides a reliable basis for further study of the effects of rainfall and earthquake on slope and disaster prevention and mitigation.

Biocompatible Gradient Chitosan Fibers with Controllable Swelling and Antibacterial Properties

Shangpeng Liu,Yi Yu,Shasha Jiang,Jiwei Li,Shuang Wang,Shaojuan Chen,Jianwei Ma 한국섬유공학회 2022 Fibers and polymers Vol.23 No.1

This study developed a partially water-soluble carboxymethyl chitosan (PWCS) fibers by ethanol-waterexhaustion method. Sodium carboxylate was successfully introduced into chitosan fibers, as evidenced by FTIR, whichenhanced natural chitosan water solubility. SEM-EDS revealed that the sodium carboxylate groups presented in a gradientdistribution from the exterior to the interior of the PWCS fibers, which leads to a controllable swelling performance. Moreover, the PWCS fibers had excellent antibacterial ability against S. aureus and E. coli bacteria comparing with origin CSfibers. The cytotoxicity assay (CCK-8) and fluorescence staining confirmed that all developed samples showed non-toxicitytowards MRC-5 and exhibited a surprising promoting effect on cell proliferation, corroborating their biocompatibility. Besides, due to its excellent controllable water selling ability and protonated amine groups, the PWCS fibers possessed anefficient hemostatic performance. In conclusion, PWCS fibers could become a promising material in hemostatic and woundhealing applications.