Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau

Wang Xungang,Hu Linyong,Liu Hongjin,Xu Tianwei,Zhao Na,Zhang Xiaoling,Geng Yuanyue,Kang Shengping,Xu Shixiao 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12

Objective: The intestinal microbiota enhances nutrient absorption in the host and thus promotes heath. Qinghai semi-fine wool sheep is an important livestock raised in the Qinghai- Tibetan Plateau; however, little is known about the bacterial microbiota of its intestinal tract. The aim of this study was to detect the microbial characterization in the intestinal tract of the Qinghai semi-fine wool sheep. Methods: The bacterial profiles of the six different intestinal segments (duodenum, jejunum, ileum, cecum, colon and rectum) of Qinghai semi-fine wool sheep were studied using 16S rRNA V3-V4 hypervariable amplicon sequencing. Results: A total of 2,623,323 effective sequences were obtained, and 441 OTUs shared all six intestinal segments. The bacterial diversity was significantly different among the different intestinal segments, and the large intestine exhibited higher bacterial diversity than the small intestine. Firmicutes, Bacteroidetes, and Patescibacteria were the dominant phyla in these bacterial communities. Additionally, at the genus level, Prevotella_1, Candidatus_ Saccharimonas, and Ruminococcaceae_UCG-005 were the most predominant genus in duodenal segment, jejunal and ileal segments, and cecal, colonic, and rectal segments, respectively. We predicted that the microbial functions and the relative abundance of the genes involved in carbohydrate metabolism were overrepresented in the intestinal segments of Qinghai semi-fine wool sheep. Conclusion: The bacterial communities and functions differed among different intestinal segments. Our study is the first to provide insights into the composition and biological functions of the intestinal microbiota of Qinghai semi-fine wool sheep. Our results also provide useful information for the nutritional regulation and production development in Qinghai semi-fine wool sheep. Objective: The intestinal microbiota enhances nutrient absorption in the host and thus promotes heath. Qinghai semi-fine wool sheep is an important livestock raised in the Qinghai-Tibetan Plateau; however, little is known about the bacterial microbiota of its intestinal tract. The aim of this study was to detect the microbial characterization in the intestinal tract of the Qinghai semi-fine wool sheep.Methods: The bacterial profiles of the six different intestinal segments (duodenum, jejunum, ileum, cecum, colon and rectum) of Qinghai semi-fine wool sheep were studied using 16S rRNA V3-V4 hypervariable amplicon sequencing.Results: A total of 2,623,323 effective sequences were obtained, and 441 OTUs shared all six intestinal segments. The bacterial diversity was significantly different among the different intestinal segments, and the large intestine exhibited higher bacterial diversity than the small intestine. Firmicutes, Bacteroidetes, and Patescibacteria were the dominant phyla in these bacterial communities. Additionally, at the genus level, Prevotella_1, Candidatus_ Saccharimonas, and Ruminococcaceae_UCG-005 were the most predominant genus in duodenal segment, jejunal and ileal segments, and cecal, colonic, and rectal segments, respectively. We predicted that the microbial functions and the relative abundance of the genes involved in carbohydrate metabolism were overrepresented in the intestinal segments of Qinghai semi-fine wool sheep.Conclusion: The bacterial communities and functions differed among different intestinal segments. Our study is the first to provide insights into the composition and biological functions of the intestinal microbiota of Qinghai semi-fine wool sheep. Our results also provide useful information for the nutritional regulation and production development in Qinghai semi-fine wool sheep.

Elastro-hydrodynamic lubrication model of multi-decked foil thrust bearing with copper wire support

Tianwei Lai,Yu Guo,Wei Wang,Shuangtao Chen,Yu Hou 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.9

Copper wire supported foil thrust bearing (CWFTB), which can be applied in high speed turbo-expander, adopts underlying flat foil as elastic supporting that interconnected with copper wires. In this paper, finite element model of the thrust bearing is developed incorporating the fluid film and foil deflections. Fluid-structure interaction (FSI) is realized through serial solution of the fluid Reynolds equation and the plate Kirchhoff equation. Pressure profile of the gas film and deflections of the top foil and bottom foil are obtained through iteration. Static bearing performance such as bearing load and friction torque of the bearing are evaluated concerning bearing configuration such as taper/land ratio, taper height and copper wire arrangements. The finite element numerical results are validated with data from experiments.

High cell density fermentation of Saccharomyces cerevisiae GS2 for selenium-enriched yeast production

Tianwei Tan,Zheng Wang,Liyang Zhang 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.6

This paper describes a fed-batch fermentation protocol about production of selenium-enriched yeast. Saccharomyces cerevisiae GS2 was selected because of its high tolerance to selenium. The strain GS2 was tested and 122±0.5 g·l−1 dry cell weight was obtained after 30 h cultivation through feed back control of feed rate of glucose according to the concentration of ethanol and dissolved oxygen. Furthermore, the optimal pattern of Na2SeO3 addition was 9 mg Na2SeO3 against 1 g DCW at late exponential phase. With the combination of glucose feeding and Na2SeO3 addition,the final dry cell biomass reached 102±0.4 g·l−1 and a Se uptake level of 2,020±13 mg·Kg−1 was achieved in a 5 l fermentor after 38 h cultivation.

Studies on Purification of 1,3-propanediol by Molecular Distillation

Zhe Wang,Zhe Wu,Tianwei Tan 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4

The separation of 1,3-propanediol using molecular distillation has been studied. The effects of operating temperature and feed flow rate through a sequential distillation strategy were investigated. The optimal experimental temperature was at 70oC for separating 1,3-propanediol and the by-product 2,3-butanediol. Meanwhile, the volume flow rate was 10 mL/min. As a result, the recovery of 1,3-propanediol and 2,3-butanediol were 87.6 and 87.5%,respectively. Furthermore, the integrated separation characteristic of 1,3-propanediol was evaluated through macrolevel and micro-level models. The separation factors of 1,3-propanediol versus 2,3-butanediol and glycerol were 0.11 and 1.07, respectively, affirming that the separation of 1,3-propanediol by molecular distillation was feasible.

An Open-switch Fault Diagnosis Method for Single-phase PWM Rectifier Based on CEEMD-DNN

Na Qin,Tianwei Wang,Deqing Huang,Yiming Zhang,Lei Ma 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.10

Based on complementary ensemble empirical mode decomposition and deep neural network (CEEMDDNN), a novel diagnosis method is proposed to discover the open-circuit faults of insulated gate bipolar transistors (IGBTs) in single-phase pulse width modulation (PWM) AC-DC rectifier, an important part of traction power supply system of high-speed railway. By virtue of the combination of signal processing and deep learning schemes, the multi-scale feature information for IGBT’s open-circuit fault diagnosis are extracted by regarding the input current of rectifier as the original signal. More clearly, CEEMD is adopted to decompose the original signal to a series of intrinsic mode function components (IMFs). Then, the correlation coefficient algorithm is used to evaluate the correlation between each of the IMFs and the original signal. Further, those IMFs that have been verified to be highly correlated with the original signal are selected as the input eigenvectors of DNN to train the IGBTs fault diagnosis network. Experimental results show that the proposed CEEMD-DNN algorithm is superior to pure DNN, empirical mode decomposition (EMD)-DNN and ensemble empirical mode decomposition (EEMD)-DNN in the sense that within the similar time-consuming situations the fault diagnosis accuracy has been improved from 73.0%, 86.0%, and 89.9% to almost 99.0%. This is mainly owing to the remarkable decomposing performance of CEEMD for complex mixed signal, and the strong feature extraction and learning abilities of DNN in pattern recognition.

Simultaneous separation of three isoflavones on oligo-β-cyclodextrin substituted polystyrene-based medium and evaluation adsorption characteristics using AutoDock

Li Yang,Tianwei Tan,Jinyi Luan,Xin Wei,Yongqiang Yang,Wenqing Huang,Zhi Guo,Yujie Wang 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.2

The adsorption characteristics between three isoflavones in crude soybean sample and styrene-β-cyclodextrin (S-CD) were studied by molecular mechanics calculations with AutoDock. The discriminatory ability exhibited by S-CD against glycitin, daidzin, and genistin through the differences in the interaction energies and complex geometries could potentially serve for the chromatographic separation. The chromatographic elution order of the three analytes on oligo-β-cyclodextrin substituted polystyrene-based medium (PS-CDP) was predicted depending on the binding free energy values obtained from molecular docking simulations. The experimental results of chromatographic evaluation on PS-CDP were consistent with the simulation prediction. The three isoflavones in sample can be simultaneously separated in one-step under the optimized mobile phase, which consisted of methanol/0.1mM NH4AC=65.0/35.0 (v/v) by PS-CDP column chromatography. A glycitin purity of 95.1% with a recovery of approximate 86.3% was achieved by proper peak cutting, and that of daidzin and genistin was 95.8%, 95.4% and 96.2%, 95.7%, respectively.

Synthesis and Enhanced Photocatalytic Activity of Black Porous Zr-doped TiO2 Monoliths

Jimin Du,Huiming Wang,Huijuan Chen,Menghe Yang,Xiaoling Lu,Hui Guo,Zhanmin Zhang,Tianwei Shang,Shujue Xu,Wenqi Li,Pianpian Wang,Imran Shakir 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.6

Black Porous Zr-doped TiO2 monoliths were successfully synthesized by a combined route including sol–gel method with polystyrene spheres as template followed by calcination at high temperature in the presence of oxygen. As-synthesized samples were then treated in hydrogen atmosphere at 500℃ for 6 h to obtain black Porous Zr-doped TiO2 monoliths. This as-synthesized black porous Zr-doped TiO2 composite showed relatively narrow bandgap compared to the pure anatase phase of TiO2, which may have its origin from the contributions from the Zr impurity and oxygen vacancies. The photocatalytic activity of the black porous Zr-doped TiO2 monolith was examined by carrying out the dye degradation on Rhodamine B under visible irradiation. Our experimental results indicate that black porous 0.9% Zr-doped TiO2 sample exhibits high photocatalytic activity with the photodecomposition of 95.25% under visible irradiation for 120 min.

Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites

Zhu Wen,Liu Tianwei,Deng Jian,Wei Cong Cong,Zhang Zi Jun,Wang Di Ming,Chen Xing Yong 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10

Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet. Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats.Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome.Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments.Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

Synthesis and characterization of a novel soil stabilizer based on biodegradable poly(aspartic acid) hydrogel

Jun Yang,Fang Wang,Tianwei Tan 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.5

A novel soil stabilizer based on poly-amino acid - polyaspartic acid (PASP) and its copolymer which modified by xanthan gum (XG) was studied to increase soil particle compressive strength and resistance to wind erosion. Due to its unique property, the stabilizer aggregated individual soil particles and formed crust. The sample compressive strength increased from 0.175 to 0.612MPa and the wind erosion modulus reduced from 22.43 to 10.56 g·m−2·min−1 after the 1% PASP hydrogel was applied by 1.67 Lm−2 (1 cm of crust). The soil water content was higher than the control due to the polymer’s excellent water-retaining property. The polymer had no negative influence on seed germination and growth. The biodegradability experiment showed that PASP was easy to biodegrade and therefore it was safe to apply in the field.

Differential Evolution Algorithm for Constraint Joint Replenishment Problem with Indirect Grouping Strategy

Li Chengyan,Gao Jun,Zhang Tianwei,Wang Xiaotian 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.5

The joint replenishment problem with deterministic resource restriction is considered. We present a differential evolution (DE) algorithm that uses indirect grouping strategy to solve constrained joint replenishment. The procedure and structure of the DE algorithm is proposed. Extensive computational experiments are performed to compare the performances of the DE algorithm with results of genetic algorithm (GA) and heuristic algorithm CRAND. The experimental results indicate that the DE algorithm performs relative to CRAND and superior to GA.