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Zhi Guo,Hongjiang Liu,Jianchu Zheng,Liugen Chen,Haiyan Yuan 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.2
Insect-proof nets (IPN) are widely used to protect crops from invertebrate pests. The effects of IPN on the main microclimate parameters of paddy fields, as well as grain quality, were investigated for three japonica Oryza sativa L. varieties (Nanjing 44, Nanjing 45, and Suxiangjing 3) that are widely grown in China. The environmental temperature of IPN-treated fields increased slightly, whereas the maximum value of photosynthetically active radiation (PAR), daily cumulative PAR, and daily cumulative global radiation all decreased. However, the ratio of PAR to global radiation increased significantly, from 15.08 to 45.48%. Compared to the no insect-proof net (NIPN) treatment, the head rice percentage increased significantly for all three varieties; however, the chalky grain percentage, area and degree, and the amylose content all decreased significantly. The response of gel consistency, protein concentration, and the main parameters of the rapid visco analyzer (RVA) profile of rice starch to IPN treatment differed between varieties. The peak viscosity and breakdown were increased compared to the NIPN treatment, whereas the final viscosity and setback were decreased. However, no significant differences were found between the IPN and NIPN treatments. Furthermore, statistical analysis indicated no interaction between rice planting pattern (RPP) and rice variety (RV) for the rice grain quality parameters. These results suggest that IPN treatment improved rice grain quality for all japonica rice varieties studied, possibly due to the slight increase in environmental temperature and increased ratio of PAR to global radiation in IPN treated paddy fields.
Zhi-Rong Zhong,Zhi-rong Zhang,Ji Liu,Yong Deng,Hong-wei Zhang,Yao Fu,Qing-guo Song,Qin He 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
A novel non-viral gene delivery system, Procationic-Liposome-Protamine-DNA complexes (PLPD) which could further adsorb transferrin on the surface as a targeting ligand to form Tf- PLPD, was prepared and characterized before and after lyophilization. The size distribution of Tf-PLPD was in the range of 240 ± 12 nm and the zeta potential was -24.10 ± 2.5 mV. The transfection efficiencies of PLPD and Tf-PLPD were 12.18 ± 3.8 and 24.26 ± 2.6 mU β-galactosidase/ mg protein respectively. The lyophilization and the presence of serum didn’t affect the tansfectivities of PLPD or Tf-PLPD. Compared to LipofectamineTM 2000 (Invitrogen, U.S.A.), the procationic liposomes had less cytotoxicity to cells. In summary the procationic lipoplex described here, combining the condensing effect of protamine and the targeting capability of Tf, was a perspective non-viral vector for gene delivery system.
Zhi-qiang Li,Dan Gui,Zhi-hua Sun,Jun-bo Zhang,Wen-zhi Zhang,Hui Zhang,Fei Guo,Chuangfu Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.4
Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DwbkA) and evaluated its virulence. The survival of 2308DwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.
Mechanical Properties and Damage Characteristics of Coal-Rock Combination with Different Dip Angles
Zhi-Guo Xia,Shuai Liu,Zhuang Bian,Jinhu Song,Fan Feng,Ning Jiang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5
The effect of the dip angle of an interlayer coal seam on rock mass mechanical behavior and damage characteristics is seldom discussed. To address the lack of the existing research, a numerical simulation was conducted using the Particle Flow Code (PFC2D), and its damage to mechanical behavior, acoustic emission (AE) characteristics, and damage characteristics were analyzed. The outcomes exhibit the peak strength and elastic modulus of coal-rock combinations are between that of rock and coal. As the coal inclination angle rises, the elastic modulus and peak strength gradually decrease. AE model experiences the initial growth period, stable growth period, and rapid decline period. The value of AE hits and the generation of the AE signal is affected by the size of the inclination angle. The degree of damage to the coal-rock combination gradually rises as the angle rises. The damage process of the coal and rock composite sample encompasses an initial damage phase, damage stable evolution phase, accelerated damage phase, and a failure phase. The damage values of the stable evolution stage for varying dip angle models are also different. As the inclination angle increases, the damage value gradually increases, while the strain at the same damage value gradually decreases.
A new experimental technique on the tubular joints of spatial grid structures
Zhi-Hua Chen,Guo-Jun Sun,Zhi-Shan Luo 국제구조공학회 2011 Structural Engineering and Mechanics, An Int'l Jou Vol.40 No.6
A new experimental technique was developed on the plate-inserted welded tubular joints of spatial grid structures. In the experimental technique, a microcomputer controlling instrument of moire intervention (MCIMI) was adopted. A test was designed on the plate-inserted welded tubular joints of spatial grid structures to show the effectiveness of the MCIMI technique. Both traditional electrical measuring technique and MCIMI technique were employed in the test. The test results showed that the MCIMI technique was feasible in the case of the complicated tests on steel structures. The MCIMI technique not only implemented the limitation of traditional electrical measuring technique, but also improved the accuracy of the test. According to the test results, we further examined the plate-inserted welded tubular joints in the cable-stayed spatial grids of the Binhai International Convention & Exhibition in Tianjin, China. The analysis showed the joints are safely designed with adequate conservatism. The research provided a new application of MCIMI in the field of large-scale structure engineering.
Guo, Miao,Liu, Chao,Qi, Feng-Jie,Zhang, Xiu-Mei,Ren, Li-Li,Liu, Yi-Meng,Meng, Zhi-Chao,Zhu, Zhi-Tu,Xiao, Jian-Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Aim: To investigate the expression of protein kinase $CK2{\alpha}$ ($CK2{\alpha}$) in human thyroid disease and its relationship with thyroid cancer metastasis. Materials and Methods: Using immunohistochemistry we measured the expression of $CK2{\alpha}$ in 76 benign and malignant human thyroid cancer tissues, including 10 pairs of papillary carcinoma tissues with or without lymph node cancerous metastasis and similarly 10 pairs of lymph nodes. Results: The expression of $CK2{\alpha}$ was found to be higher in thyroid carcinoma cases (papillary carcinoma, follicular carcinoma, anaplastic carcinoma and medullary carcinoma) than in ones such as chronic lymphocytic thyroiditis, nodular goiter and adenoma. These findings were also confirmed by RT-PCR and Western blotting. More strikingly, elevated expression of $CK2{\alpha}$ in thyroid papillary carcinoma tissues was not only significantly associated with lymph node cancerous metastasis and clinical stage of thyroid cancers; but also correlated with epithelial-mesenchymal transition (EMT) and high tenascin C (TNC) expression. In addition, EMT and high TNC expression in thyroid carcinoma tissues was significantly associated with lymph node cancerous metastasis. Conclusions: Elevated expression of nuclear $CK2{\alpha}$ is a poor prognosis indicator in lymph node cancerous metastasis of human thyroid cancers.