Hydrothermal Synthesis of CrO2 Nanoparticle Arrays on Ti nano-pit Array Templates

Xiaoling Wang,Lu Wang,Tao Lin,Hailiang Huang,Gehui Wen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.1

Chromium dioxide (CrO2) nanoparticle arrays were fabricated on Ti nano-pit array templates by hydrothermal synthesis using CrO3 aqueous solution as precursor. The Ti nano-pit array template was obtained by stripping TiO2 nanotube array formed on the Ti foil. CrO2 nanoparticles are arranged in honeycomb pattern in a large area and their surface density reaches 1.5 x 10 10 cm -2. The CrO2 nanoparticle arrays show typical magnetic behavior, and the easy axis is parallel to the plane of Ti nano-pit array template. The influence of the concentration of CrO3 aqueous solution on the CrO2 nanoparticle arrays is studied and the mechanism of the formation of the CrO2 nanoparticle arrays is briefly discussed.

The effects of main anoxic section oxidation-reduction potential on the metabolism of PHA and TP in continuous-flow single-sludge treatment system

Xiaoling Wang,Hai Lu,Tiehong Song,Ke Zhao 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.3

The experimental results and material balance analysis in this paper revealed the regularity of poly-hydroxy alkanoates (PHA) and total phosphorus (TP) metabolism in a continuous-flow single-sludge wastewater treatment system under different main anoxic section oxidation-reduction potential (ORPan) conditions. We also evaluated the effectiveness of the operation control parameters of ORPan as the continuous-flow single-sludge sewage treatment system from the aspect of the reaction mechanism. Using a programmable logic controller (PLC) automatic control system to take the circulating flow in nitrification as the controlled variable based on the feedback control structure, an experimental study was carried out under the condition of ORPan setting value of 143mV, 123mV, 105mV, 95mV, 72 mV and 57mV, respectively, with other operational design parameters remaining unchanged. Influent water quality of chemical oxygen demand/total nitrogen (COD/TN) was 5.0±0.6. The results showed that when ORPan was set at 95mV, the maximum values of PHA synthesis and storage rate, PHA degradation rate, phosphorus release rate and phosphorus absorption rate in anaerobic and pre-anoxic segments were 82.34, 7.90, 47.31, 14.27, 1.50 and 8.52mg/ (L·h), respectively. According to the metabolic mechanism of PHA and TP, ORPan was further proved to be the operation control parameter of the continuous-flow single-sludge sewage treatment system, and when the COD/TN value was 5.0±0.6, the optimal setting value was 95mV.

Achieving safe and high-performance gastrointestinal tract spectral CT imaging with small-molecule lanthanide complex

Xiaoling Che,Chunmei Yang,Liping Pan,Didi Gu,Guidong Dai,Jian Shu,Lu Yang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Non-intrusive imaging of gastrointestinal (GI) tract using computed tomography (CT) contrast agents is of the most significant issues in the diagnosis and treatment of GI diseases. Moreover, spectral CT, which can generate monochromatic images to display the X-ray attenuation characteristics of contrast agents, provides a better imaging sensitivity for diagnose inflammatory bowel disease (IBD) than convention CT imaging. Methods Herein, a convenient and one-pot synthesis method is provided for the fabrication of small-molecule lanthanide complex Holmium-tetraazacyclododecane-1, 4, 7, 10-tetraacetic acid (Ho-DOTA) as a biosafe and high-performance spectral CT contrast agent for GI imaging with IBD. In vivo CT imaging was administered with both healthy mice and colitis mice induced by dextran sodium sulfate. Results We found that Ho-DOTA accumulated in inflammation sites of large intestines and produced high CT contrast compared with healthy mice. Both in vitro and in vivo experimental results also showed that Ho-DOTA provided much more diagnostic sensitivity and accuracy due to the excellent X-ray attenuation characteristics of Ho- DOTA compared with clinical iodinate agent. Furthermore, the proposed contrast media could be timely excreted from the body via the urinary and digestive system, keeping away from the potential side effects due to long-term retention in vivo. Conclusion Accordingly, Ho-DOTA with excellent biocompatibility can be useful as a potential high-performance spectral CT contrast agent for further clinical imaging of gastrointestinal tract and diagnosis of intestinal system diseases.

Use of nitrate-nitrogen concentration for controlling source, cellular matter production and oxygen consumption for sewage treatment

Xiaoling Wang,Xiaoyu Zhang,Hai Lu 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.2

Carbon saving, oxygen consumption reduction and cellular matter production reduction of Modified University of Cape Town (MUCT) process under different nitrate-nitrogen concentration in the main anoxic section was studied. This was investigated by material balance analysis, biochemical reaction process and its metrology of ordinary heterotrophic bacteria, denitrifying bacteria, nitrifying bacteria and phosphorus-accumulating bacteria. The flow and distribution of carbon, nitrogen, and oxygen in the MUCT, and the influence of the regulation of the c(NO3) on the carbon source, cellular matter production, and oxygen consumption of the process were explained in detail. In the programmable logic controller (PLC) automatic control system, the circulating flow rate of nitrate was set as the controlled variable. Adopting the feedback control structure, c(NO3) was altered at 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0mg·L1, respectively. In this experimental study, the quality of influent and other operation design parameters remained unchanged. The results showed that the effluent quality was at its best when c(NO3) was controlled at 2.0- 4.0mg/L. Again, the distribution of chemical oxygen demand (COD) in the anaerobic section was between phosphorus- accumulating bacteria, common heterotrophic bacteria and denitrifying bacteria, and the distribution was related to c(NO3). Due to this phenomenon, the distribution of nitrate-nitrogen between denitrifying bacteria and denitrifying phosphorus-accumulating bacteria, and poly-hydroxy alkanoates (PHA) between denitrifying phosphorus-accumulating bacteria and aerobic phosphorus-accumulating bacteria was changed. Carbon source of 110.0 kg acetic acid/103m3 sewage was saved, while the cell material output was reduced by 37.5%, and the oxygen consumption of 51.1 kg O2/ 103m3 sewage was reduced. In the MUCT process, the regulation of c(NO3) enhanced the denitrifying phosphorus uptake performance of the main anoxic section and obtained good carbon source savings, reduction of cellular matter production, and reduction of oxygen consumption.

Variation in the quantity and composition of phosphorus accumulating organisms in activated sludge driven by nitrate-nitrogen

Wang Xiaoling,Shi Chunyan,Pan Wenbo,Lu Hai,Zhang Xiaoyu 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.7

Anaerobic/anoxia sequencing batch reactor (A/ASBR) system was used to analyze the quantity and composition of each branch of phosphorus accumulating organisms (PAOs) in activated sludge under different nitrate-nitrogen (NO 3 − -N) concentrations by using real-time quantitative polymerase chain reaction (PCR) technology. The study determined whether NO 3 − -N and its concentration change were the main driving factors for the variation of the quantity and composition of each branch of PAOs. The results show that with the increase of NO 3 − -N concentration from 10 mg/L to 40 mg/L, the number of bacterial 16S rRNA genes in the A/ASBR reactor changed slightly at 6.81×1011–7.53×1011 copies/g dry sludge. The number of PAO genes (Acc 16S rRNA) increased from 1.98×1011 to 3.53×1011 copies/g dry sludge, and the total number of ppk1 genes increased from 1.25×1011 to 3.59×1011 copies/g dry sludge. Additionally, the number of polyphosphate kinase (ppk) genes in Accumulibacter branch IA, IIC and IID was high, and the changes were positively related to the concentration of NO 3 − -N, while the number of branches in IIA, IIB and IIF was very low. The dosing concentration of NO 3 − -N was the main driving factor for the change of PAOs and their branch number and composition in the A/ASBR reactor.

Polymorphisms in the Perilipin Gene May Affect Carcass Traits of Chinese Meat-type Chickens

Zhang, Lu,Zhu, Qing,Liu, Yiping,Gilbert, Elizabeth R.,Li, Diyan,Yin, Huadong,Wang, Yan,Yang, Zhiqin,Wang, Zhen,Yuan, Yuncong,Zhao, Xiaoling Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.6

Improved meat quality and greater muscle yield are highly sought after in high-quality chicken breeding programs. Past studies indicated that polymorphisms of the Perilipin gene (PLIN1) are highly associated with adiposity in mammals and are potential molecular markers for improving meat quality and carcass traits in chickens. In the present study, we screened single nucleotide polymorphisms (SNPs) in all exons of the PLIN1 gene with a direct sequencing method in six populations with different genetic backgrounds (total 240 individuals). We evaluated the association between the polymorphisms and carcass and meat quality traits. We identified three SNPs, located on the 5' flanking region and exon 1 of PLIN1 on chromosome 10 (rs315831750, rs313726543, and rs80724063, respectively). Eight main haplotypes were constructed based on these SNPs. We calculated the allelic and genotypic frequencies, and genetic diversity parameters of the three SNPs. The polymorphism information content (PIC) ranged from 0.2768 to 0.3750, which reflected an intermediate genetic diversity for all chickens. The CC, CT, and TT genotypes influenced the percentage of breast muscle (PBM), percentage of leg muscle (PLM) and percentage of abdominal fat at rs315831750 (p<0.05). Diplotypes (haplotype pairs) affected the percentage of eviscerated weight (PEW) and PBM (p<0.05). Compared with chickens carrying other diplotypes, H3H7 had the greatest PEW and H2H2 had the greatest PBM, and those with diplotype H7H7 had the smallest PEW and PBM. We conclude that PLIN1 gene polymorphisms may affect broiler carcass and breast muscle yields, and diplotypes H3H7 and H2H2 could be positive molecular markers to enhance PEW and PBM in chickens.

Preparation of anhydrous from red gypsum and effect of high strength gypsum on its properties

Changrong Liu,Lu Wang,Hongbin Tan,Faqin Dong,Xiaoling Ma,Feihua Yang 한양대학교 청정에너지연구소 2022 Journal of Ceramic Processing Research Vol.23 No.6

Red gypsum waste comes from titanium dioxide production by sulphuric acid method. Anhydrite was prepared from thewaste. The effects of calcined temperature on the properties of anhydrite were studied. The normal consistency of anhydritedecreased with the increase of calcined temperature, while the compressive strength firstly increase and then decrease. Theeffect of high strength gypsum on the properties of anhydrite was also studied. The normal consistency of sample decreasedwith the increase of high strength gypsum content, while the setting time firstly decrease and then increase, strength anddensity increased.

Recrystallization and Microstructural Evolution During Hot Extrusion of Mg97Y2Zn1 Alloy

Bin Chen,Xiaoling Li,Chen Lu,Dongliang Lin 대한금속·재료학회 2014 METALS AND MATERIALS International Vol.20 No.3

This study revealed that the extrusion temperature has a great influence on microstructure and mechanicalproperties of the Mg97Y2Zn1 alloy. The average grain sizes increased from 3 μm to 8 μm with increasingextrusion temperatures from 623K to 773 K. Both dynamic recrystallization (DRX) and static recrystallization(SRX), which occur during and after deformation, respectively, were observed. The alloy, which extrudedat a relatively high temperature, exhibited lower strength because the strain strengthening was balanced bythe softening that originated from DRX. Three types of morphologies, namely, big recrystallized grains, fine recrystallizedgrains, and non-recrystallized grains, were observed in the extruded microstructures obtained at 623 K. The dislocation density was quite high in the fully recrystallized grain. The extruded microstructures obtainedat 773 K were composed of large grains with more uniform size. Their degree of recrystallization was higherand the dislocation density also declined. All dislocation in the grain were distinguished as <c+a> dislocations. Submicron scale precipitates were distributed along the newly formed recrystallized grain boundaries andhad a remarkable pinning effect on the recrystallized grain growth after extrusion at 773 K. The precipitates canbe divided into two main types: mixed type and single type.

Interaction Between Serum/Glucocorticoid-Regulated Kinase 1 and Interleukin-6 in Chronic Rhinosinusitis

Lai Yuting,Hu Li,Yang Lu,Hu Xianting,Song Xiaole,Yang Jingyi,Li Hongbin,Chen Kun,Li Huabin,Wang Dehui 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.5

Purpose: Serum/glucocorticoid-regulated kinase 1 (SGK1) has recently emerged as a critical regulator of inflammatory diseases. In this study, we examined SGK1 expression and its possible pathogenic roles in chronic rhinosinusitis (CRS). Methods: Immunohistochemistry, western blotting, Bio-Plex assay, enzyme-linked immunosorbent assays, and quantitative real-time polymerase chain reaction were performed to assess protein and gene expression levels. The mRNA expression levels of SGK1 and interleukin-6 (IL-6) were extracted from a CRS database to perform correlation analysis. Stable cell lines with SGK1 overexpression (16HBE) and knockdown (A549) were constructed to investigate the interaction between SGK1 and IL-6 in vitro. Results: SGK1 exhibited strong cytoplasmic and nuclear staining in the epithelial layers and the lamina propria of nasal polyps (NPs) and in the mucosal tissues of CRS without nasal polyps (CRSsNP). The mRNA and protein expression levels of SGK1 and IL-6 were significantly increased in NPs and CRSsNP tissues, compared to control tissues. SGK1 phosphorylation was significantly greater in NPs than in CRSsNP tissues (P < 0.01). The mRNA levels of SGK1 and IL-6 were significantly correlated (P < 0.001, r = 0.649). Exposure to IL-6 significantly increased SGK1 expression in cultured dispersed NP cells, 16HBE cells, and A549 cells. IL-6 expression was significantly down-regulated in SGK1-overexpressing 16HBE cells (P < 0.01) and significantly up-regulated in SGK1-knockdown A549 cells (P < 0.05). Administration of GSK650394, a SGK1 inhibitor, significantly increased IL-6 self-induced mRNA expression in cultured dispersed NP cells and 16HBE cells. Conclusions: The interaction between SGK1 and IL-6 may play an anti-inflammatory role in IL-6-induced inflammation in the pathogenesis of CRS.

Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa

Li, Chunjin,Sun, Yongfeng,Yi, Kangle,Li, Chengjiao,Zhu, Xiaoling,Chen, Lu,Zhou, Xu Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.10

The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR experiments using RNA extracted from bovine spermatozoa with SRY-specific primers yielded a 456 bp product, but the amount of SRY mRNA in sperm was lower than that in the testes (p<0.01). A protein of approximately 27 KDa was detected by western blotting. The SRY transcript was detected in the midpiece of approximately half the spermatozoa by in situ hybridization, and the SRY protein was detected in the heads of half the spermatozoa by immunofluorescence, indicating that SRY mRNA and protein may only be present in Y-bearing spermatozoa. These results suggest that the SRY transcript and protein are present in bovine ejaculated Y-sperm. The roles of the SRY gene in spermatogenesis, sperm motility, and the sperm-oocyte interaction merit further investigation.