GAS OF 96 PLANCK COLD CLUMPS IN THE SECOND QUADRANT

Zhang, Tianwei,Wu, Yuefang,Liu, Tie,Meng, Fanyi American Astronomical Society 2016 The Astrophysical journal Supplement series Vol.224 No.2

<P>Ninety-six Planck cold dust clumps in the second quadrant were mapped with (CO)-C-12 (1-0), (CO)-C-13 (1-0), and (CO)-O-18 (1-0) lines at the 13.7 m telescope of Purple Mountain Observatory. (CO)-C-12 (1-0) and (CO)-C-13 (1-0) emissions were detected for all 96 clumps, while (CO)-O-18. (1-0) emissions were detected in 81 of them. Fifteen clumps have more than one velocity component. In the 115 mapped velocity components, 225 cores were obtained. We found that 23.1% of the cores have non-Gaussian profiles. We acquired the V-lsr, FWHM, and T-A of the lines. Distances, T-ex, velocity dispersions, N-H2, and masses were also derived. Generally, turbulence may dominant the cores because sigma(NT)/sigma(Therm) > 1 in almost all of the cores and Larson's relationship is not apparent in our massive cores. Virial parameters are adopted to test the gravitational stability of cores and 51% of the cores are likely collapsing. The core mass function of the cores in the range 0-1 kpc suggests a low core-to-star conversional efficiency (0.62%). Only 14 of 225 cores (6.2%) have associated stellar objects at their centers, while the others are starless. The morphologies of clumps are mainly filamentary structures. Seven clumps may be located on an extension of the new spiral arm in the second quadrant while three are on the known outer arm.</P>

Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites

Zhu Wen,Liu Tianwei,Deng Jian,Wei Cong Cong,Zhang Zi Jun,Wang Di Ming,Chen Xing Yong 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.10

Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet. Objective: The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats.Methods: In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome.Results: The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments.Conclusion: Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.

Characterization of the bacterial microbiota across the different intestinal segments of the Qinghai semi-fine wool sheep on the Qinghai-Tibetan Plateau

Wang Xungang,Hu Linyong,Liu Hongjin,Xu Tianwei,Zhao Na,Zhang Xiaoling,Geng Yuanyue,Kang Shengping,Xu Shixiao 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12

Objective: The intestinal microbiota enhances nutrient absorption in the host and thus promotes heath. Qinghai semi-fine wool sheep is an important livestock raised in the Qinghai- Tibetan Plateau; however, little is known about the bacterial microbiota of its intestinal tract. The aim of this study was to detect the microbial characterization in the intestinal tract of the Qinghai semi-fine wool sheep. Methods: The bacterial profiles of the six different intestinal segments (duodenum, jejunum, ileum, cecum, colon and rectum) of Qinghai semi-fine wool sheep were studied using 16S rRNA V3-V4 hypervariable amplicon sequencing. Results: A total of 2,623,323 effective sequences were obtained, and 441 OTUs shared all six intestinal segments. The bacterial diversity was significantly different among the different intestinal segments, and the large intestine exhibited higher bacterial diversity than the small intestine. Firmicutes, Bacteroidetes, and Patescibacteria were the dominant phyla in these bacterial communities. Additionally, at the genus level, Prevotella_1, Candidatus_ Saccharimonas, and Ruminococcaceae_UCG-005 were the most predominant genus in duodenal segment, jejunal and ileal segments, and cecal, colonic, and rectal segments, respectively. We predicted that the microbial functions and the relative abundance of the genes involved in carbohydrate metabolism were overrepresented in the intestinal segments of Qinghai semi-fine wool sheep. Conclusion: The bacterial communities and functions differed among different intestinal segments. Our study is the first to provide insights into the composition and biological functions of the intestinal microbiota of Qinghai semi-fine wool sheep. Our results also provide useful information for the nutritional regulation and production development in Qinghai semi-fine wool sheep. Objective: The intestinal microbiota enhances nutrient absorption in the host and thus promotes heath. Qinghai semi-fine wool sheep is an important livestock raised in the Qinghai-Tibetan Plateau; however, little is known about the bacterial microbiota of its intestinal tract. The aim of this study was to detect the microbial characterization in the intestinal tract of the Qinghai semi-fine wool sheep.Methods: The bacterial profiles of the six different intestinal segments (duodenum, jejunum, ileum, cecum, colon and rectum) of Qinghai semi-fine wool sheep were studied using 16S rRNA V3-V4 hypervariable amplicon sequencing.Results: A total of 2,623,323 effective sequences were obtained, and 441 OTUs shared all six intestinal segments. The bacterial diversity was significantly different among the different intestinal segments, and the large intestine exhibited higher bacterial diversity than the small intestine. Firmicutes, Bacteroidetes, and Patescibacteria were the dominant phyla in these bacterial communities. Additionally, at the genus level, Prevotella_1, Candidatus_ Saccharimonas, and Ruminococcaceae_UCG-005 were the most predominant genus in duodenal segment, jejunal and ileal segments, and cecal, colonic, and rectal segments, respectively. We predicted that the microbial functions and the relative abundance of the genes involved in carbohydrate metabolism were overrepresented in the intestinal segments of Qinghai semi-fine wool sheep.Conclusion: The bacterial communities and functions differed among different intestinal segments. Our study is the first to provide insights into the composition and biological functions of the intestinal microbiota of Qinghai semi-fine wool sheep. Our results also provide useful information for the nutritional regulation and production development in Qinghai semi-fine wool sheep.

DENSE GAS IN MOLECULAR CORES ASSOCIATED WITH<i>PLANCK</i>GALACTIC COLD CLUMPS

Yuan (袁敬华,), Jinghua,Wu, Yuefang,Liu, Tie,Zhang, Tianwei,Li, Jin Zeng,Liu, Hong-Li,Meng, Fanyi,Chen, Ping,Hu, Runjie,Wang, Ke American Astronomical Society 2016 The Astrophysical journal Vol.820 No.1

<P>We present the first survey of dense gas toward Planck Galactic Cold Clumps (PGCCs). Observations in the J = 1-0 transitions of HCO+ and HCN toward 621 molecular cores associated with PGCCs were performed using the Purple Mountain Observatory's 13.7 m telescope. Among them, 250 sources were detected, including 230 cores detected in HCO+. and 158 in HCN. Spectra of the J = 1-0 transitions from (CO)-C-12, (CO)-C-13, and (CO)-O-18 at the centers of the 250 cores were extracted from previous mapping observations to construct a multi-line data set. The significantly low detection rate of asymmetric double-peaked profiles, together with the good consistency among central velocities of CO, HCO+, and HCN spectra, suggests that the CO-selected Planck cores are more quiescent than classical star-forming regions. The small difference between line widths of (CO)-O-18. and HCN indicates that the inner regions of CO-selected Planck cores are no more turbulent than the exterior. The velocity-integrated intensities and abundances of HCO+ are positively correlated with those of HCN, suggesting that these two species are well coupled and chemically connected. The detected abundances of both HCO+ and HCN are significantly lower than values in other low- to high-mass star-forming regions. The low abundances may be due to beam dilution. On the basis of an inspection of the parameters given in the PGCC catalog, we suggest that there may be about 1000 PGCC objects that have a sufficient reservoir of dense gas to form stars.</P>