組織素 分解酵素를 生成하는 高溫性 곰팡이의 分離 및 特性

성낙계,강인수,박채규,정영철,김두현 慶尙大學校 1990 論文集 Vol.29 No.1

고온에서 잘 생육하고 cellulose 및 xylan 분해력 이 우수한 곰팡이를 분리할 목적으로 pH5.0, 50℃, Czapek-Dox pulp 배지에서 celluase complex와 xylanase생산이 우수한 곰팡이 H-702균주를 최종선정하여 균학적 성상을 조사한 결과, Aspergillus속과 거의 일치하였으며, 본 공시균주가 효소 생산에 미치는 영향과 효소학적 성질의 결과를 요약하면 다음과 같다. 생육 및 효소생성의 최적온도는 50℃이고, 균체증식 최적 pH는 5.0, cellulase 생산 최적 pH 는 4.5 xyanases는 4.0으로 나타났으며, 효소최대 생성 배양 4일째에, 균체증식은 약 3일경에 최대에 도달하였다.또한 α-1, 4-linkage를 가진 섬유성 물질 왕성하였으며 glucose 와 같은 저분자물질에서는 효소생합성이 현저히 저해되었는데 이런 결과로 볼때 이균주는 carbon catabolite repression을 받고 있었다. 효소생산에 적합한 탄소원으로는 α-cellulose,avicel, filter paper 등 이였으며, 질소원으로는 암모니아가 효과적이었으며 무기염류는 Ca??, Mg??, Mn??, Co??에서 효소생합성이 좋은것으로 나타났다. 계면활성제는 0.15% Tween 80이 좋았다. 개선된 KD배지에서는 각종 효소활성도가 1.2-1.6배 증가하였으며 또한 KD배지에서 각종탄소원을 지질로하여 효소 생합성을 조사한 결과 3% a-cellulose 에서 효소활성도가 좋았고 값싼 기질인폐신문지에서도 효소활성이 높게 나타났다. 조효소의 최적 온도는 cellulas complex 는 70℃, xylanase는 60℃로 나타났으며 열안정성은 cellulas complex 70℃까지는 30분간 , 80℃에서는 잔존활성이 약 93%였고 xylanase는 70℃에서도 거의 안정하였다.최적 pH는 cellulas complex는 5.0, xylanase는 5.5였으며, pH안정성은 3.0-8.0까지 안정하였다.조효소의 미치는 금속이온의 영향은 Ca??,Mg?? Mn??에의해 활성화 되었으며 Pb??, Cu??, Mg?? 에 의해 현저히 저해 현상을 나타냈다. In order to isolate a fungus which grow well in the high temperature and has the excellent decomposing power of cellulose and xylose a fungus. H-702 which produced potently cellulase complex(CMCase,avicelase and β-glucosidase) and xylanase in Czapek-Dox pulp medium (pH 5.0, 50℃) was finally selected. Then as a result of its bacteriological properties investigated it was almost the same properties as Apergillus sp.. The effect influencing to the enzyme production of the isolated strain and enzymological properties are followed. The optimal temperature and pH for cell growth and enzyme production were 50℃, 5.0, 4.5 and 4.0.respectively . The highest production of enzyme was shown when the culture time was three days and the greatest growth of cell was shown about after two days. In the cellulosic materials(CMC.avicel and α-cellulose etc.) containing α-1.4-linking the vigorous production if enzyme was shown and in the small molecular substance such as glucose was remarkably repressed. According to these results, this stain was under the control of carbon catabolite repression.α-cellulose , avicel and filter paper were suitable for carbon source in the enzyme production and NH? for nitrogen source and Ca??, Mg??, Mn?? and Co?? for inorganic salt. 0.15% Tween 80 was a good surface active agent. In improved KD medium each enzyme activity was increased 1.2 to 1.4 times. As a result of investigating enzyme biosynthesis, which each carbon source was used as substrate in the KD medium, it was shown a good enzyme activity. The optimal temperature of crude enzyme in cellulase complex and xylanase were 70 ℃and 60℃, respectively. Thermal stability was shown for 30 minutes until 70℃in cellulase complex and its residual activity at 80℃ was about 93%. It almost was stable at 70℃in xylanase. The optimal pH for cellulase complex and xylanase were 5.0 and 5.5.respectively and they were stable in the range of pH 3.0 TO 8.0. The crude enzyme was activated by Ca?? , Mg??and Mn?? but inhibited by Pb??, Cu?? and Ag?? remarkably.

고 무기질 음료(π-water) 섭취시 럭비선수들의 근력, 근 지구력 및 유산소성 운동능력에 미치는 영향

손두옥,선우섭,윤우상,유재충,박영진,오종환 경희대학교 사회체육연구소 1996 體育科學論叢 Vol.- No.9

Most of elite sports player during the sports competing and trainning have used the mineral supplements to enhance on the human performance and, also a number of researcher have recommended the mineral supplements of RDA(Recommended Dietary Allowance) for them. However, It has not exactly known to the scientific interpretation and evidences of relationship between human performance and mineral. Thence, The purpose of this study was to prove whether the mineral supplements of high RDA could enhance the sport player's performance or not The subjects of this study were composed of sixteen Rugby football players(experimental group=8, high density multimineral water intake for 30days; control group=8, placebo intake)in K-H University. Both of groups were tested three times(pre=before intake, mid=after 15days supplements, post=after 30days supplments) about maximum anaerobic power(MAnp) and maximum oxygen uptake by bicycle ergometer(868), muscle strength and muscle endurance by isokinetic Cybex system(350). The statistical analysis employed this study was t-test by statistic 512+ program in machintosh computer. The result of this study were appeared that the difference in control and experimental group signified that the experimental group was high more than the control group in left knee extensor's peak torque at mid-test(P<.05), in left knee extensor's total work(%BW) at mid-test(P<.05), in left extensor's average power and average power(%BW) at mid-test(P<.05). Relative maximal oxygen uptake(㎖/LBMkg/min) in experimental group was higher than control group after 30 days the mineral supplements. These results suggest that the high density multimineral water intake should influence partially rugby football player's performance by the increment of muscle endurance and maximal oxygen uptake.

性別 및 年齡에 따른 體格과 體力의 特性과 變化에 關한 縱斷的 硏究Ⅱ : Emphasised on College Men of Department of Physical Education 體育專功 大學生을 中心으로

金尙國,姜東洹,張周鎬,金昇一,徐再均,金耕知,孫斗玉,鮮于攝,崔榮烈,尹宇相,金亨燉,方永鎭,朴英震,柳在忠 慶熙大學校 體育科學大學 1989 體育科學論叢 Vol.- No.2

The purpose of this study was to compare the characteristics, change in physique and physical function of college students majoring physical education and Taekwondo (454 males and 64 females of age groups ranging from 18 to 26). The results are as following: 1. The male group of 23yrs old in physical education showed highest values in 7 items(Weight, Chest Girth, Fore Arm Girth, Thigh Girth, Calf Girth, Scapular Skinfold Thickness and Suprailiac Skinfold Thickness). 2. In the Physical function, the male group of 18yrs old showed highest values in Power, Flexibility, and then 21yrs old at Back Strength, Vital Capacity, and 23yrs old at Grip Strength, and 25yrs old at Blood Pressure, respectively. 3. In the physique, the male group of 22yrs old showed highest values in Weight, Chest Girth, Thigh Girth, Calf Girth, and then 24yrs old Upper Arm Girth, Fore Arm Girth, and 19yrs old at Height, Upper Limb Length, respectively. 4. In Physical Function, the 22yrs old showed highest values in Back Strength, Vital Capacity, and then 23yrs old at Side Step, Trunk Extension, but 18yrs old showed lower Values in Grip Strength, Side Step, Vital Capacity and Blood Pressure. 5. The female group of 22yrs old showed highest values in 8 items(Height, Sitting Height, Waist Girth, Upper Limb Length, Lower Limb Length, Skinfold Thickness), and then 18yrs old at 5 items (Weight, Upper Arm Girth, Fore Arm Girth, Thigh Girth, Calf Girth), respectively. 6. In the physical function, the 22yrs old showed highest Values in Grip strength, Back Strength, Power, 5 Minute Run, and 18yrs old at flexibility, Harvard Step Test, respectively. 7. In the change of the physique for two years(1988-1989) in the male group, majoring Physical education the 22yrs old showed increase in 4 items, and the 20yrs old in 3 items, but 18yrs old showed decrease in 3 items. 8. In the change of the physical function for two years in the male group, the 20yrs old showed increase in 4 items, and the 21, 25, 26yrs old in 3 items, but the 19yrs old showed decrease in 6 items, and 18, 22 yrs old in 5 items, respectively. 9. The change of the physique for two years in all female group, of 18, 19, 20, 21yrs old showed increase in 11 items except Skinfold Thickness. 10. In the change of physical function for two years in female group, the 18yrs old showed increase in 3 items, and then 20yrs old in 5 items, but 19, 22yrs old decreased 12 items, respectively. 11. The highest significant difference in physique between male and female was found in weight(mean Value 17.5%), and the lowest difference was found Thigh Girth, Calf Girth(1,3%), Upper Limb Length, Lower Limb Length(2.5%). 12. The Highest difference in physical function was Strength (mean Value 40%), Vital Capacity (33%), Power(22%), but Lower Trunk Flexion, Foot Balance(3.4%). 13. In the difference of Physique and physical function between physical education major and taekwondo major, the physical education showed higher values in skinfold Thickines (mean Value 16.5%), Weight, Waist Girth(4.7%) and Strength(33%), Endurance(5.6%), but subjects majoring Taekwondo showed higher values in Flexibility and Foot Balance. 14. In all male groups, Waist Girth, Scapular Skinfold Thickness, Back Strength, Sargent Jump, Trunk Extension and Diastolic Blood Pressure were showed a significantly related at 0.001 level.

高品質 豚肉 生産을 위한 고구마 사일리지의 開發에 關한 硏究 : 3. 肥肉豚에 대한 고구마 사일리지 給與가 豚肉의 品質에 미치는 影響 3. Effects of Supplementing the Sweet Potato Silage on Pork Quality

安瑛敏,高永杜,文勝式,陳詳根,鄭孟和 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

육에 대한 소비자들의 욕구는 계속해서 증가하고 다양해지고 있어 이러한 욕구에 부응하기 위해 육의 풍미 및 육색을 증진시키는 것으로 알려진 고구마 사일리지를 급여한 돈육 일반성분과 물리적인 성질 및 도체특성을 조사하였다. 고구마 사일리지 제조는 고구마의 외부에 부착된 흙을 수세하여 마쇄한 후 고구마 사일리지 1은 고구마 70%, 맥강 20%, 대두박10%를 혼합하여 제조하였으며, 고구마 사일리지 2는 고구마 70%, 맥강 30%를 혼합하여 제조하였다. 일반성분으로는 수분, 조지방, 조단백, 조회분을 조사하였고, 물리적 성질로는 pH, 보수성, 연도, 육과 지방색, 관능검사, 도체특성을 조사하였다. 일반성분에서 수분은 고구마 사일리지 2 급여구가 다른 처리구에 비하여 유의적으로 높게 나타난(P<0.05) 반면에 조지방 함량과 조단백질 함량은 유의적으로 낮은 함량을 보였으며, 그리고 조회분 함량은 처리구간에 유의적인 차이가 없었다. 물리적 성질인 pH의 변화는 대조구와 고구마 사일리지 급여구간에는 유의적인 차이가 없었다. 보수성은 고구마 사일리지 급여구가 대조구보다 유의적으로 높게 나타났으며(P<0.05), 연도는 고구마 사일리지 급여구가 대조구에 비하여 좋은 결과를 보였다. 육색의 변화는 고구마 사일리지 급여로 명도가 유의적으로 낮게 나타났으며, 적색도와 황색도는 측정부위에 따라 값의 변화가 심하였다. 지방색은 고구마 사일리지 급여가 대조구에 비하여 황색도가 낮아 좋은 결과 였다. 관능검사 결과는 고구마 사일리지 급여가 대조구에 비하여 풍미는 유의적으로 높은 성적을 보였지만(P<0.05), 다즙성, 견고성, 탄력성에는 유의적인 차이가 없었다. 전체적인 관능검사 성적은 고구마 사일리지 2 급여구가 가장 좋은 성적을 얻었다. 도체특성에 미치는 변화를 측정한 결과 도체중은 유의적으로 낮게 나타났으나(P<0.05), 지육율은 차이가 없었다. 그러나 고구마 사일리지 급여로 등지방 두께가 유의적으로 감소하는 결과를 보였다(P<0.05). 본 실험 결과 보수성과 연도는 대조구에 비해 고구마 사일리지 급여구가 더 나은 결과 나타내었으며, 육색과 관능검사, 도체특성 결과도 대조구에 비교해 고구마 사일리지 급여구가 유의적으로 더 나은 것으로 나타나 고구마 사일리지를 급여시 특히 고구마 사일리지 1 급여구의 경우 돈육의 육질을 크게 향상시킬 수 있을 것으로 사료된다. The present study was conducted to investigate the effect of supplementary feeding of sweet potato silage on carcass and its meat quality in finishing gilts. Two sweet potato silages containing same ingredients with different ratios(silages 1 and 2; 70 and 70% sweet potato, 20 and 30% barley bran, 10 and 0% soybean meal, respectively) were fermented for 21 days in 500 kg-capacity silos. Finishing gilts were fed a commercial finishing diet(CONT), or the same basal diet plus 0.4kg silage 1(TRT 1) or silage 2(TRT 2)/head/day beginning from 90kg of body weight, 18 heads per group, up to approximately 110kg of final weight and slaughtered. After measuring carcass traits, loin eye sections were removed and their physicochemical characteristics were analyzed. The sensory evaluation on the fresh and cooked meat was performed by the panel test and the whole experiment was repeated 3 times. Backfat thickness was reduced in TRT 1 and TRT 2 compared with CONT, but carcass weight and dressing percentage were not different between the feeding regimens. Compared with CONT, TRT 1 and TRT 2 had greater water holding capacity, tenderness, fat color and palatability scores including flavor and reduced brightness, with no difference in pH, juiciness, firmness and gumminess. Overall, TRT 2 exhibited the greatest sensory evaluation score.

SLAP Ⅱ 병변의 진단을 위한 관절 조영 자기 공명 영상에서 견관절 위치에 따른 비교

이영수,신동배,박수진,김진용,김희상,하두회 대한견.주관절학회 2000 大韓肩.주關節學會誌 Vol.3 No.2

高品質 豚肉 生産을 위한 고구마 사일리지의 開發에 關한 硏究 : 2. 肥肉豚에 대한 고구마 사일리지 給與가 生産形質과 經濟性에 미치는 影響 2. Effects of Feeding the Sweet Potato Silage on Performances and Production Cost of Finishing Pigs

宋瑛敏,高永杜,文勝式,金斗煥,鄭孟和 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

본 연구는 고구마 사일리지를 이용한 고품질 돈육 생산을 위하여 고구마 사일리지가 돼지에 대한 생산형질과 경제성에 미치는 영향을 조사하였다. 사양시험은 고구마 사일리지를 고구마 70, 맥강 20, 대두박 10의 비율(사일리지 1)과 고구마 70, 맥강 30의 비율(사일리지 2)로 500kg 용 사일로에 충진하여 21일간 발효시켜 제조하고, 급여시기를 각각 체중이 75kg 과 90kg부터 시작하여, 급여시간은 각각 32일과 25일로서 체중 110kg까지 사육하며, 동일한 교배조합(LYD)으로 생산된 육돈 암컷을 3반복, 반복당 18두씩 총 324두를 공시하였으며, 고구마 사일리지 급여량을 하루에 두당 0.4kg씩 공급한 결과는 다음과 같다. 1. 고구마 사일리지를 급여한 경우 개시 체중 75kg과 90kg 공히 종료시 체중, 증체량, 일당증체량 및 사료효율은 배합사료를 무제한 급여하는 대조구 보다 유의적으로 낮았고, 배합사료의 섭취량과 일당사료섭취량은 유의적으로 적게 섭취하였으나, 고구마 사일리지를 건물로 환산하여 합산한 총사료섭취량은 대조구와 고구마 급여구간에 비슷하게 나타났다. 2. 고구마 사일리지를 급여한 경우 배합사료의 섭취량은 줄어들어 배합사료비가 절감되나 고구마 사일리지를 섭취함으로서 총사료비는 비슷하다. kg 증체에 소요되는 사료비는 높게 나타났고, 배합사료급여를 기준한 생산 지수는 고구마 사일리지 급여구가 40∼50% 이상의 사료비가 추가로 소요되는 것으로 분석되었다. This study was carried out to investigate the effects of supplementing sweet potato silages on live performance and production cost in finishing gilts. Two sweet potato silages containing same ingredients with different ratios(silages 1 and 2; 70 and 70% sweet potato, 20 and 30% barley bran, 10 and 0% soybean meal, respectively) were fermented for 21 days in 500 kg-capacity silos. Finishing gilts were fed a commercial finishing diet(CONT), or the same basal diet plus 0.4kg silage 1(TRT 1) or silage 2(TRT 2)/head/day beginning from either 75kg or 90kg of body weight up to approximately 110kg of final weight and the whole experiment was repeated 3 times. 1. TRT 1 and TRT 2 were lesser than CONT in final weight, total weight gain, average daily gain, feed efficiency and total and daily basal diet intake, but not in total dry matter intake including the silage. 2. Compared with CONT, TRT 1 and TRT 2 showed a reduced basal diet cost resulting from the reduced intake of it, but total feed cost was not different between the feeding regimens. Total feed cost per kg weight gain, however, was increased 40∼50% by supplementing the silage.

Representation of bounded Linear Operators on Reproducing Kernel Hilbert Space

변두원,이성희,박달원,노영순,김승동 공주대학교 과학교육연구소 2001 과학교육연구 Vol.32 No.1

재생핵 힐버트공간에서 유계인 작용소는 어떤 적분변환의 형태로 표시된다. 또한 재생핵을 이용하여 어떤 적분변환의 형태가 유계인 작용소이기 위한 필요충분조건을 구한다. The bounded linear operators on a reproducing kernel Hilbert space can be represented in the form of generalized integral transform. Also, they are completely characterized by integral kernels.

알코올 금단 발작 환자에서 진전 섬망 예방에 대한 Nimodipine과 Chlordiazepoxide의 효과 비교

정두신,양광익,이보람,방차옥,이태경,성기범,안무영,박형국 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

Background & objectives : An increase of calcium influx during alcohol withdrawal state may contribute to neuronal hyperexitability, which cause delirium tremens(DT). Some investigators have found calcium channel blockers effects in prevention of DT in experimental animals. Therefore we evaluated the preventive effects of calcium channel blocker(nimodipine) and benzodiazepine(chlordiazepoxide) on the development of DT in alcohol withdrawal seizure(AWS) patients. Method : A total 59 patients with AWS were divided into three groups according to the management nimodipine-treated, chlordiazepoxide-treated, and control(no specific medication) groups. We compared the incidence rates of DT in the three groups. Results : Total incidence rate of DT was 30.5%(18 of 59 patients). There were 6 DTs of 18 patients(33.5%) in nimodipine-treated group, 4 of 21 patients(22.5%) in chlordiazpoxide-treated group, and 8 of 20 patients(44.0%) in control. Conclusion : The control group(44.0%) showed the highest incidence rate of DT. And nimodipine-treated(33.5%) and chlordiazepoxide-treated group(22.5%) were followed. However, this result failed to demonstrated statistically significant differences due to small numbers size.

업무 관련성 견갑상 포착 신경증이 의심되는 3례

정두신,성기범,신현길,안무영,김형수,홍영의 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : Entrapment of the suprascapular nerve is frequently overlooked in the differential diagnosis of shoulder pain. Methods : Suprascapular entrapment neuropathy is a well-defined clinical entity and EMG and NCV is used to confirm a diagnosis. But the diagnosis typically not considered until patients develop severe weakness secondary to atrophy of the spinous musculature that the nerve supplies. Results : A narrow suprascapular notch has rarely been reported as a work-related factor of this entrapment neuropathy. Diagnosis of suprascapular entrapment neuropathy is based on the patients' clinical course, neurologic, radiologic, and electrophysiologic findings. One of the most helpful evaluations was anteriorposterior projection with the X-ray tube angled 15-30 degree caudally. The suprascapular entrapment neuropathy is relatively uncommon entity of shoulder discomfort(pain, weakness, and atrophy). Conclusions : If the worker who used his shoulder joint repetitiously having the shoulder pain and muscle weakness, we must rule out the suprascapular entrapment neuropathy. And it is needed to evaluate the motions which cause suprascapular entrapment neuropathy as the ergonomic factor.

性別 및 年齡에 따른 體格과 體力의 特性과 變化에 關한 縱斷的 硏究 Ⅰ : 體育專攻 大學生을 中心으로 Emphasised on College Men of Physical Education

金尙國,姜東洹,金昇一,徐再均,金耕知,孫斗玉,鮮于攝,崔榮烈,尹宇相,金亨燉,方永鎭,李錫元 慶熙大學校 體育科學大學 1988 體育科學論叢 Vol.- No.1

The purpose of this study was to compare the characterists and change in physique and physical function by college student of physical education major(287males and 40 females of age groups ranging from 18 to 26). The results were as following; 1) The characteristics of physique and physical function by sex and age. ① The male group of 18yrs old showed higher values in Height, Weight, Upper Limb Length, Strength, Flexibility, and Vital Capacity than any other groups, but female group showed lower values in Height, Weight, Chest Girth, Lower Limb Length, Thigh, Girth, Calf Girth and all of the Physical function. ② The male group of 19yrs old showed high values in power and Agility, then female group showed in Strength and Flexibility. ③ The male group of 20yrs old showed the lower values in Weight, Waist Girth, Upper Limb Length, Fore Arm Girth, Thigh Girh.Calf Girth, Vital Capacity, and female group are very lowed in Upper Arm Lenght, Fore Arm Length, Skin Fold Thickness and 5 minute Run. ④ The male group of 23yrs old showed the high values in Upper Arm Girth, Fore Arm Girth and Balance, and then female group higher in Weight, Chest Girth, Waist Girth, Upper Arm Girth, Fore Arm Girth, Thigh Girth, Power, Shuttle Run, Balance and vital capacity than any other age Groups. ⑤ In male group, Chest Girth, and Blood Pressure were significantly related, and Side Step and Trunk Extension were nagatively related to the age at 0.1% level, and then in female group, Sargent Jump, Standing Long Jump and Vital Capacity were showed a significantly related at 0.5% level. 2) The difference of physique and physical function between male and female by age. ① Everage difference in whole physique items except skinfold thickness was 9.8%, and that 21.1% in all of physical function except Trunk Flextion between male and female group subjects. ② About the ages, the 18yrs old male group showed the 22.8% higher values of 26 items, but 22yrs old male group were about 12.2% in 23 items, thus it is very lower than any other groups. 3) The difference of physique and physical function between General group and physical education group in age-matched. ① In the physique male physical education groups showed about 7% higher in Weight, Chest Girth, Upper Limb Length, Upper Arm Girth, Thigh Girth, and female group showed about 7% higher in Weight and Upper Limb Length. ② In the physical function, Trunk Flextion, 5 minute Run, and Blood Pressure(Diastolic) were about 8% high values in whole group, and female group showed about 8% higher in Standing Long Jump, Shuttle Run, and Trunk Extension. ③ In 22yrs old male and female physical education group were very dominated in physique and physical function than the other age group against the general group.