全蝎 抽出物이 血栓症, 全腦虛血 및 腦細胞毒性에 미치는 影響

백명현,황영근,정지천,강정준,김성훈 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

본 실험은 전갈 추출물이 어혈병태 모델과 KCN으로 유발된 전뇌허혈, 뇌세포독성 등에 미치는 영향을 관찰하였다. 전갈은 Dextran에 의해 유도된 혈전으로 감소된 혈소판수, fibrinogen량, hemocrit치를 유의성 있게 증가시키고. 증가된 prothrombin time을 유의성 있게 단축시켰다. 전갈은 thrombin과 ADP에 의해 유도된 혈소판 응집을 억제하였으나 collagen에 의해 유도된 혈소판 응집에는 저해효과를 나타내지 않았다. 또한 collagen과 epinephrine에 의해 유도된 pulmonary embolism에 대하여 보호 효과를 나타내었다. 전갈은 KCN에 의한 전뇌허혈 유발 실험에서 혼수시간을 유의성 있게 단축시켰고, Amyloid β protein(25-35)에 의해 유도된 PC12 세포의 독성에 대하여 보호효과를 나타내었다. This following is effect of Buthus martensi Karsch (BMK) extract on dextran-thrombus model, KCN-induced coma, cytotoxicity of brain etc. BMK extract significantly increased number of platelet and fibrogen and significantly shortened the prothrombin time as compared with control group treated with dextran. BMK extract didn't affect the changes of hematocrit as compared with control group treated with dextran. BMK extract induced a significant inhibition of human platelet aggregation induced by thrombin and ADP but did not affect human platelet aggregation induced by collagen. BMK extract showed a protective effect on pulmonary thrombosis induced by collagen and epinephrine. BMK extract prolonged the duration of KCN-induced coma and showed a protective effect on cytotoxicity of PC12 cells induced by amyloid β protein(25-35) in a dose dependent manner. These results suggested that BMK extract might be usefully applied for prevention and treatement of thrombosis and brain damage.

원형질체 융합에 의한 Aspergillus nidulans의 효소생산

백종현,김성준,양영기 조선대학교 기초과학연구소 1988 自然科學硏究 Vol.11 No.1

Aspergillus nidulans FGSC 473, 154 각각의 원형체질 형성 최적 조건을 구해서 FGSC 473(pyr^-,rib^-)과 FGSC 154(ade^-, met^-)의원형질체 융합을 통하여 얻은 hybrid 균주의 효소 활성도를 모균주의 효소활성도와 비교하였다. 각각의 실험결과는 다음과 같다. 원형질체 형성은 0.5% Novozym 234의 세포벽 분해효소를 0.6MKCl의 삼투압 안정체에 녹여 37℃에서 2시간 동안 반응시켰을 때 최적으로 나타났다. 원형질체 융합의 빈도는 0.22%~0.51%로 나타났다. 모균주와 hybrid균주간의 세포외 효소활성도를 비교해본 결과 hybrid 균주는 CMCase만이 모균주 보다 높았고 다른 효소들은 대체로 모균주보다 낮은 효소 활성도를 나타냈다. Formation of protoplasts from auxotrophes of Aspergillus nidulans FGSC 473 and 154 by Novozym 234, and their protoplast fusion between complementary mutants were investigated. From Aspergillus nidulans FGSC 473 (pyr^-, rib^-) and 154 (ade^-, met^-), hybrid strains (No. 1-13 Strain) were isolated by protoplast fusion. Enzyme activities of each of Aspergillus nidulans 473, 154 and hybrid strains (No. 1-13 strain) were investigated. The main results of the present study were as follows: Optmimal concentration and reaction time of the lytic enzyme for protoplasts were 5㎎/㎖ and 2hr respectively. As osmotic stabilizer for protoplast formation, 0.6M KCl was to be suitable. The fusion frequencies between complementary strains ranged from 0.22% to 0.51% . In Cellulaseand Xylanase system, the most of enzyme activities of hybrid strains were lower than those of parental stains, but only CMCase activities of hybrid strains were higher than those of parental strains.

혼합유화제 비율에 따른 인계난연제 캡슐의 제조 및 특성 연구

백경현,이준영,홍상현,김중현 한국공업화학회 2004 응용화학 Vol.8 No.1

The microcapsules containing triphenyl phosphate (TPP) flame retardant have been prepared by using the epoxy resin (Novolac Type) with good physical properties and network structure. The reactants including TPP, epoxy resin and mixed surfactants were emulsified to oil in water (O/W) by the phase inversion technology and then conducted on the crosslinking of epoxy resin by In-situ polymerization. The capsule size and size distribution of TPP microcapsules was controlled by mixed surfactant ratio. The thermal property of TPP capsules were measured by the TGA. The morphology and capsule size were also investigated by the SEM respectively. TPP capsules with 1 : 1 of mixed surfactant (F127 : SDBS) ratio were more spherical and uniform than it with others. The TPP capsule size was 12~17㎛ and TGA results shows that TPP capsule efficiency was about 73%.

허혈 조건부여가 심근기능의 회복에 미치는 영향 : 적출 심장모델을 이용한 실험연구 Isolated heart experimental study

백광제,김준식,한승백,전영진,이인성 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Brief episode of coronary artery occlusion(i.e.,ischemic preconditioning) makes the heart more resistant to injury from a subsequent ischemic insult. Although a great deal of effort has been made in studying ischemic preconditioning, the underlying mechanism of ischemic preconditioning and its effect on hypothermic insult has not been elucidated. This study was performed to see whether ischemic preconditioning protects against the depression of cardiac contractility induced by hypothermic cardioplegic arrest/reperfusion. And recently, adenosine was known to have some correlation with the mechanism of preconditioning. If so, does this effect remain after the blockade of adenosine receptor by 8-phenyl theophyline? Method: Twenty-four Sprague-Dawley rat weighed 250-350g were used and divided into three groups. Rat hearts were removed rapidly, and each isolated heart paced with a rate of 180/min was perfused by modified Krebs-Hensleit buffer(KHB) solution on a Langendorff apparatus for an hour. After obtaining baseline data including left ventricular pressure(LVP), dp/dt, and coronary flow, cardiac arrest was induced by perfusion of 0℃ crystalloid cardioplegic(St Thomas) solution. After that, all hearts were stored in the same St Thomas solution at same temperature for 2 hours. In group Ⅰ(control group), the heart was reperfused by KHB solution. In group Ⅱ(preconditioning group), the heart was subjected to two 2-minute episode of global ischemia followed by 5 minute reperfusion with KHB solution(preconditioning) before cardiac arrest. In group Ⅲ(phenyl theophylline group), the heart was subjected to preconditioning procedure and 8-phenyl theophylline at 10μM in concentration was added to KHB solution at time of reperfusion. Observing parameter was obtained in each group at 10, 20, 40 and 60 minutes after starting reperfusion and compared statistically by use of one way ANOVA test(STASTICA, release 4.5). p-value less than 0.05 was considered significant. Results: Although depressed LVP, dp/dt, and coronary flow were seen in all groups during the reperfusion period, the preconditioned group showed more effective recovery of LVP than that of the control group, especially at 10, 20 and 40 minutes(p<.05). We failed to demonstrate the difference between the phenyl theophylline group and the control group(p=NS). Conclusion: These results suggest that ischemic preconditioning has protective effect on recovery state of hypothemic cardioplegic arrest/reperfusion. Its protective effect was limited during early reperfusion stage and was blocked by adenosine blocker.

상행결장의 거대지방종으로 인한 대장중첩증 1예 : Case Report

백무준,김태윤,김성용,이문수,김창호,조무식 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Colonic lipoma is rare and mostly asymptomatic lesions; but as they become larger they may produce abdominal pain, constipation, diarrhea, hemorrhage, and intussusception. We report the case of a 61-year-old male who sufferd from nonspectific recurrent abdominal pain in the right upper and lower quadrants. Abdominal computed tomography (CT) revealed a constipating colonic tumor. The patient underwent an operative resection with primary anastomosis. His postoperative course was unremarkable, and he was discharged to home on postoperative day 11. Surgical pathologic examination comfirmed a large lipoma.

일부 남성 금속제품 조립작업자들의 피로에 영향을 미치는 작업관련요인

장준호,강동묵,고상백,김정원,조병만,이수일 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.2

목적 : 남성 금속제품 조립 작업자들의 피로에 영향을 미치는 작업관련요인을 파악하고자 한다. 방법 : 작업자의 일반적 특성 및 작업관련 특성, 육체적 부하, 직무 스트레스 등을 조사 항목으로 하여 자동차 부품 조립업 1개소(476명), 자동차 엔진 조립업 1개소(610명), 대형 선박 엔진 조립업 1개소(454명)의 작업자(1,540명)를 대상으로 수거된 설문지 중 부실하게 응답한 38부를 제외한 1,136부(총연구대상의 73.8%)를 분석하였고, 피로도와의 연관성을 다중선형회귀분석으로 살펴보았다. 조사는 자기 기입식 설문지를 사용하였다. 육체적 부하를 측정하기 위해서는 Borg의 scale이 사용되었다. 격무 스트레스를 측정하기 위해서는 Karasek의 Job content questionnaire가 사용되었다. 피로의 측정은 전반적 피로도(sum of fatigue), 일상생활 기능장애(daily dysfunctioning), 상황적 피로(situational fatigue), 피로점수의 총합(sum of fatigue)으로 구성되어 있는 다면적 피로척도를 사용하였다. 피로점수의 분포는 본 연구에 응답한 피로점수의 총합을 사분위수로 나누어서 4등급으로 나누었다. 결과 : 피로점수의 분포는 피로점수의 총합을 사분위수로 나누었을 때, 0≤1등급≤73, 74≤2등급≤82, 83≤3등급≤93, 94≤4등급≤133이었다. 다중선형회귀분석에서 피로점수의 총합과 유의한 관계를 보인 경우는 주간근무, 긴 주당 노동시간, 높은 육체적 부하, 높은 직무요구도, 낮은 직무자율성, 낮은 상사의 지지 등이었고, 전반적 피로도와 유의한 관계를 보인 경우는 주간근무, 높은 육체적 부하, 높은 직무요구도, 낮은 직무자율성, 낮은 상사의 지지 등이었다. 일상생활 기능장애와 유의한 관계를 보인 경우는 주간근무, 긴 주당 노동시간, 높은 육체적 부하, 낮은 직무자율성 등이었다. 상황적 피로와 유의한 관계를 보인 경우는 높은 연령, 주간근무, 긴 주당 노동시간, 낮은 직무자율성 등이었다. 결론 : 피로관련 요인은 긴 주당노동시간, 높은 직무요구도, 높은 육체적 부하, 낮은 직무자율성, 낮은 상사의 지지로 생각된다. Objectives: To find work related factors affecting perceived fatigue among male metal product assemblers. Methods: The study subjects were metal product assemblers from three metal product companies (476 car parts assemblers, 610 car engine assemblers, 454 ship engine assemblers). From the total of 1,540 subjects, 1,136 (73.8%) were included and 38 respondents with insufficient replies were excluded. Associations between fatigue score and other variables (general characteristics, job related factors, workload, job stresses) were investigated in terms of the concept of multidimensional fatigue construct by self administered questionnaire. Using MFS (Multidimensional Fatigue Scale), JCQ (Job Content Questionnaire), and Borg's scale, we estimated the fatigue, job stress, and workload, respectively. Fatigue score was estimated by 'sum of fatigue' in MFS. Associations were tested by linear regression analysis using SAS v.8.0. Results: In this study, the fatigue related factors were older age, longer working hours per week, higher workload, higher job demand, lower decision latitude, and lower supervisor support. Conclusions: Prolonged working hours, higher workload, higher job demand, poor supervisor support and lower decision latitude were fatigue related factors among the male metal product assemblers of this study.

건강한 여성의 자궁경부 배양 결과 분석

박성은,백진영,강명서,어영,윤갑준 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.6

참박과 호박 종자의 발아촉진을 위한 저선량 감마선의 효과

김재성,백명화,이영근,이혜연,유준철 한국환경농학회 2002 한국환경농학회지 Vol.21 No.3

참박과 호박 채종종자의 발아율을 증진시킬 수 잇는 종자 처리 방법을 확립하고자 저선량 감마선을 1∼20 Gy 까지 조사하여 발아와 초기생육 및 생리활성을 관찰하였다. 저선량 조사한 참박과 호박종자의 발아율은 대조구보다 증가하는 경향을 보였는데 효과는 종자상태에 따라 다르게 나타났다. 참박 70∼80% 발아율종자의 발아는 4∼20 Gy에서 증가하였는데 특히 4 Gy조사에서 가장 높았고, 80∼90% 발아율종자도 4 Gy 조사에서 효과를 보였다. 파종 4일 후의 발아율은 참박 70∼80%와 80∼90% 발아율 종자의 경우는 4 Gy조사에서 대조구보다 각각 66%와 26% 증가하였고, 호박의 70∼80% 발아율종자는 2 Gy 조사에서, 80∼90% 발아율종자는 8 Gy 조사에서 향상되었다. 저선량 조사한 유묘생육은 발아율이 증가한 선량에서 증가하였는데 특히 호박 70∼80% 종자의 2 Gy 조사에서 가장 높았다. 파종 6일 후에 조사한 참박의 효소활성은 4 Gy조사시에 단백질과 catalase 활성이, 호박의 경우는 catalase와 peroxidase 활성이 2 Gy와 8 Gy 조사에서 증가하였다. 참박과 호박종자의 발아와 유묘생육 및 생리활성은 저선량 감마선 조사에 의해 촉진됨을 확인할 수 있었다. To establish the seed treatment method of the gamma radiation for germination enhancement of bottle gourd(Lagenaria siceraria Standl) and pumpkin(Cucurbita ficifolia Bouche), seeds were irradiated with the dose 1~20 Gy of the gamma radiation. The germination rate in irradiated group was higher than that of the control, depending on seed condition. In the case of bottle gourd, the germination rate of 70~80% germinative seed was increased at 4-20 Gy irradiated groups. Especially, it was the highest at 4 Gy irradiated group. The germination rate of 80~90% germinative seed was the highest at 4 Gy irradiated group. The germination rate of 70~80% and 80~90% germinative seed at 4 days after sowing was increased 66% and 26% at 4 Gy irradiated group, respectively. In the case of pumpkin, the germination rate of 70~80% and 80~90% germinative seed was increased at 2 Gy and 8 Gy irradiated group, respectively. Effect of the low-dose gamma radiation on the early growth was enhanced depending on their germination enhancement. Especially, it was the highest at 2 Gy irradiated group of 70~80% gen inative seed of pumpkin. Catalase activity of bottle gourd was increased at 4 Gy irradiated group. Catalase and peroxidase activity of pumpkin were increased at 2 Gy and 8 Gy irradiated group. These results suggest that the germination, early growth and physiological activity of bottle gourd and pumpkin seeds could be promoted by the low dose gamma radiation.

치수 및 치근단병소에서 Prostaglandin E₂, 6-keto-prostaglandin F₁α, Leukotriene B₄의 분포에 관한 연구

임성삼,백승호,손원준 大韓齒科保存學會 2000 Restorative Dentistry & Endodontics Vol.25 No.2

구조물의 안전진단을 위한 충격파의 주파수 영역 탐사에 관한 연구

김형준,이상철,서백수 강원대학교 산업기술연구소 2005 産業技術硏究 Vol.25 No.B

Impact seismic wave method is a method for non-destructive testing of concrete structure using of stress wave which is propagate and reflected from internal flaws within concrete structure and external surface. In this study, we performed frequency domain method using impact seismic wave test for safety diagnosis of civil engineering structure. And reflection method which is used for one-dimensional target such as tunnel lining and transmission method are compared with each other.