흰쥐 난소에서 가지돌기세포 (dendritic cell) 및 큰포식세포의 특징과 분포양상

윤식,최연주,고서영,박선미,박봉수 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.1

Paraquat 중독환자의 초기검사로서 sodium dithionite를 이용한 소변내 paraquat검출의 임상적 의의

윤갑준,임경수,이진웅,김영식,이부수,박덕우,김선만,이강현,황성오,안무업 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Mortality from paraquat intoxication depends upon plasma paraquat concentration. To know the severity of paraquat intoxication is important for directing therapeutic modality and predicting prognosis. Sodium dithionite test for urinary paraquat provides an easy and simple method to determine the severity of paraquat intoxication in emergency department. purpose : To determine whether the result urinary paraquat test by sodium dithionite can predict outcome in patients with paraquat intoxication in emergency department. Subjects : 48 patients(male 31, female 17, mean age 37 years) who had exposure to paraquat and presented within 24 hours after exposure. Result : Thirty five patients were positive in paraquat urine test and thirteen patients were negative. Clinical manifestations were more severe in positive patients than in negatives. Complication was much more in positives than in negatives. 28 of 35 patients(80%) in positives and 2 of 13 patients(15%) in negatives died. Conclusion : Positive test for urinary paraquat is associated with high mortality and morbidity from paraquat intoxication, and qualitative test for urinary paraquat by sodium dithionite is an useful method to determine the severity of paraquat intoxication in emergency department.

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

가시오갈피 열매 추출물의 항돌연변이원성 및 세포독성 효과

전윤영,최승필,이효진,문선영,이득식,함승시 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

가시오갈피(Eleutherococcus senticous Maxim) 열매의 에탄올 추출물 및 분획물의 항돌연변이성 및 항암활성을 규명하였다. 항돌연별이원성 실험결과에서는 직접변이원인 MNNG, 4NQO 그리고 간접변이원인 B(α)P, Trp=P-1에 대해서 농도의존적인 돌연변이 억제활성을 나타내었다. MNNG (0.4㎍/plate)의 경우 S. typhimurium TA100 균주에서는 시료농도 200㎍/plate에서 클로로포름 분획물을 제외한 모든 분획물들에서 90%이상의 높은 억제효과를 나타내었다. 4NQO(0.15㎍/plate)에 대한 S. typhimurium TA98 균주의 경우 물 분획물의 경우 88.3%, TA100 균주에서는 에틸 아세테이트 분획물이 84.4%의 억제효과를 보였다. 간접변이원의 경우 B(α)P(10㎍/plate)에선느 에탄올 추출물과 핵산 분획물은 시료농도 200㎍/plate에서 각각 96.1%와 97.5%로 가장 높은 억제효과를 나타내었다. 그리고 Trp-P-1(0.15㎍/plate)에서는 TA100 균주의 경우 200㎍/plate 농도에서 에탄올 추출물과 물 분획물이 각각 95.5%와 90%로 높은 억제율을 보여주었고, TA98 균주에 대해서는 에틸아세티에트 분획물이 88.3%로 비교적 높은 억제효과를 나타내었다. 인간의 암세포인 폐암세포(A549), 위암세포(AGS), 간암세포(Hep3B), 유방암세포(MCF-7)에 대한 세포독성 억제 효과를 검토한 결과 모든 암세포에서 각분획물들은 1㎎/mL 농도에서 60% 이상의 비교적 높은 암세포 성장 효과를 나타내었다. 특히 MCF-7에서 높은 암세포 성장억제 효과를 나타내었는데 핵산 분획물(1㎎/mL)에서 92.7%의 높은 암세포 성장 억제 효과를 나타내었고 Hep3B에서는 부탄을 분획물(1㎎/mL)에서 82%의 비교적 높은 성장 억제효과를 나타내었다. This study was carried out to investigate the antimutagenic and cytotoxic effects of Eleutherococcus senticosus Maxim fruits ethanol extract on Salmonella typhimurium TA98, TA 100 and cancer cell lines using Ames test and SRB assay, respectively. They were extracted with ethanol and then fractionated with hexane, chloroform, ethyl acetate, butanol and water to get active fractions. In the Ames test, most of the extract had strong antimutagenic effects against the mutagenesis induced by MNNG, 4NQO, B(α)P Trp-P-1. The ethanol extract (200 ㎍/plate) of Eleutherococcus senticosus fruits showed 87.2% inhibitory effect on the mutagenesis induced by MNNG against TA100. And also, The suppression ratio against B(α)P and AGS, MCF-7, Hep3B), the value value of inhibition were mostly above 60% for each fraction (1 ㎎/mL). Hexane fraction (1 ㎎/mL) against showed the strongest cytotoxic effects of 92.7% compared to those of other fraction and butano fraetion against Hep3B was relatively high growth inhibitory effect of 82%.

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

부산대학교 신입생의 자아 정체감에 영향을 미치는 요인

전윤식,이성해,정영홍,박선자,김상구 釜山大學校 學生生活硏究所 1988 硏究報 Vol.24 No.1

The purpose of this study was to explain the factors that effect on ego-identity of Pusan National University(PNU) freshmen. In order to achieve the above purpose 2,743 freshman class of 1988 were selected. Statistical methods applied were general descriptive statistics, Pearson's collelation method, multiple regression analysis. The statistical computations were done by SPSS. The results of this study are summarized as follows: 1. The personal and domestic factors that effect on ego-identity of PNU freshman class of 1988 are mother's attitude of rearing the adaptation confidence of the university life, religion, the participation of the social reality, the experience of the acquaintance with the other sex, the self-conceit and health. 2. The personality factors that effect on ego-identity of PNU freshman class of 1988 are the social introversion and the social responsibility. 3. The factors that effect on ego-identity of PNU freshman class of 1988 are the social introversion, the social responsibility, mother's attitude of rearing religion, the participation of the social reality, a sense of superiority, the experience of the acquaintance with the other sex, the adaptation confidence of the university life.

부산대학교 재학생의 학업성취에 영향을 미치는 요인

전윤식,이성해,정영홍,박선자,김상구 釜山大學校 學生生活硏究所 1988 硏究報 Vol.24 No.1

The purpose of this study was to explain the factors that effect on a academic achievement of Pusan National University(PNU) students. In order to achieve the above purpose, two research problems were established as follows: 1. What are the personal-internal factors which effect on a academic achievement of PNU students? 2. What are the personal-exteranl factors which effect on a academic achievement of PNU students? Statistical methods applied were general descriptive statistics, Pearson's correlation method, multiple regression analysis. The statistical computations were done by SPSS. The results of this study are summarized as follows: 1.The personal-internal factor that effect on a academic achievement of PNU students is the learning skill. 2. The personal-external factors that effect on a academic achievement of PNU students are professor's fulfilling prophecy and the times of review of studies.

간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.

알레르기 환자에서 특이알레르겐 항체 양성율과 관련요인

이선화,남해선,이성수,안현철,황규윤,박준수,,박춘식 순천향의학연구소 2002 Journal of Soonchunhyang Medical Science Vol.8 No.2

Recently, allergic diseases are very prevalent in Korea, resulting in physical and economic losses during the life. It is well-known that the principle therapy is identification, eradication, and desensitization of allergens. There are many allergic diagnostic tools for identification of specific-allergens. However, none of them is satisfied with its efficiency due to lack of validity. Recently MAST-CLA has been widely used in the allergic clinical fields because of its simplicity and simultaneous identification of allergens. This study was designed to determine the positivity of allergen-specific antibodies using MAST-CLA in patients with allergic diseases, and evaluate the efficacy for allergen detection, compared to results of skin prick test. During June 2000 - September 2001, 325 study subjects visited Soonchunhyang University Chunan Hospital for the evaluation of allergic problems. Among them, 289 subjects were diagnosed with allergic disease and included for the study. Thirty four subjects visited children's allergic unit were evaluated specific allergens using a skin prick test at the same time. Our result as follows; 1. The positive rates of allergen-specific antibodies and total IgE were 51.2% and 73.4%, retrospectively. 2. There was significant difference in positive rate in age group, showing highest in teen ages(73.6%), but insignificant difference in sex in allergen-specific antibodies. 3. The positive rate was 60.4% in allergic rhinitis, 51.3% in allergic dermatitis, 46.9% in bronchial asthma, and 39.1% in urticaria in order(P=0.073). 4. Significant difference in month or season was observed, with highest rate in April(78.1%) and May(72.1%). The highest season was in spring(62.7%), and then summer(52.8%), fall(50.3%), and winter(41.4%) in order (P=0.04). 5. The three highest allergen-specific antibodies were D. pteronyssinus(39.3%), D. farinae(38.3%), and house dust(24.3%) in Korean inhalant pannel, However, in food pannel types of allergen-specific antibody were different as D. farinae(18.5%), Alternaria(18.5%), house dust(17.3%). 6. There were significant agreements between MAST-CLA and Skin prick test (D. farianae Kappa=0.636;D. pteronyssinus Kappa=0.78, both P<0.001) In conclusion, MAST-CLA could detect allergen-specific antibodies in patients with allergic diseases as a simple and valid tool of screening of allergy. Especially, it could be a useful measure of multiple allergens simultaneously in children However, more detailed allergens and valid assay for unmeasured allergens should be identified for Korean-specific allergens.

승모판대치술후 도플러 심초음피를 이용한 인공 판막의 종류에 따른 최대혈류속도와 평균혈류속도에 관한 연구

박선우,김권배,김윤년,김기식 啓明大學校 醫科大學 1988 계명의대학술지 Vol.7 No.2

The conventional non-invasive and invasive technique have limitations in evaluating cardiac prosthetic valves and artificial valves. We have used continuous wave Doppler echocardiography to evaluate 48 patients with tissue and mechanical mitral valves implanted within 4 months prior to examination. The results are as follows. 1) Of these patients, male are 28. The mean ages of male are 35.60 (yr) and female are 41.82. 2) The differences of peak and mean velocities of prosthetic acardiac valves between male and female are not significant, but the differences of valvular size between male and female are significant(male:31.00±1.30(mm), female:29.36±2.11, p<0.01). 3) The difference of size and flow velocities between mechanical (Edwards-Duromedics) and tissue(Ionescue-Shiley) valves are not significant, but the ages of patients with mechanical valves are significantly lower than tissue valves(mechanical valve; 34.05±11.28, tissue valve; 43.62±11.26, p<0.01). 4) The differences of flow velocities between mechanical and tissue valves, sizes of which are 31mm, are significant statistically (peak velocity; 130.15±24.59 vs 110.00±19.41, mean velocity; 74.49±23.14 vs 51.38±12.71, p<0.01). 5) The sizes of valves are larger, the peak and mean velocities are lower, but it is not significant statistically. The results indicated that Doppler echocardiography is a useful method for evaluating function of prosthetic valves.