Rat의 복강내에 투여한 5-fluorouracil-polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구

강대영,송규상,최정목,노승무,정경수,오정연,김진향,양준묵,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

For more effective intraperitoneal chemotherapy, the authers made 5-fluorouracil-polyglycolic acid composite disks(FU-PGA disks) with 5-fluorouracil and polyglycolic acid, a biodegradable polymer. We inserted the FU-PGA disk into the peritoneal cavites of Sprague-Dawley male rats, the control and three experimental groups; one FU-PGA disk insert group(300mg/kg), two FU-PGA disk insert group(600mg/kg), and three FU-PGA disk insert group(900mg/kg). The control group received a similar number of PGA disk inserts. A pharmacokinetic study was performed to measure the 5-fluorouracil concentration in the peritoneal fluid, blood, and tissues(liver, kidney and heart) at 24 hours, 72hours and 168 hours after insertion of the FU-PGA disk. Light and electron microscopic studies were done. The results were as follows: 1) The number of white blood cells and platelets decreased after FU-PGA insertion. The degree was proportional to the duration and amounts inserted. The change in the number of red blood cells varied slightly. 2) Light microscopically, slight changes were noted at 168 hours in the 3 disk insert group (900mg/kg). Mild fatty change and hepatocyte degeneration around the central veins of the liver were noted, with vacuolar degeneration in the proximal convoluted tubules of the kidney. 3) Electron microscopically,the liver showed focal increases of lipid droplets in the hepatocytes, and irregular nuclear membrane with focal nucleolar segregation of the fibrillar and granular elements. Also the double membranous structure of the hepatocytes and Kupffer cells was widened with bleb formation. The kidney showed wide separation of the nuclear double membrane in the proximal convoluted tubular cells and mesangial cells, with vacuolation and myelin figure formation in the mitochondria of the proximal convoluted tubular cells, at 72 hours and 168 hours in the 2 to 3 FU-PGA insert groups. The heart showed focal loss of cristae,vacuolation and myelin figure formation in the mitochondria of the 2 to 3 FU-PGA insert groups. In conclusion, despite the large amont of FU-PGA inserted, the histopathological changes in the liver, kidney and heart were slight, and consonant with the very low amount of 5-fluorouracil concentrations detected in the liver, kidney and heart. The above results suggest that the FU-PGA composite can serve as a new device for releasing drugs in a controlled manner and easily targeted to intraperitoneal organs. This device can improve the efficacy of intraperitoneal chemotherapy for gastric cancer.

오리 網膜에 存在하는 神經節細胞의 數와 分布에 關한 硏究

姜翰秀,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

The number and distribution of the retinal ganglion cells in the 1-week-old and 7-week-old ducks were determined from whole flat mounted preparations stained with methylene blue and thionin. The results were summarized as follows. 1. In the 1-week-old and 7-week-old ducks, the areas of the retinas were 269.4㎟ and 357.2㎟, respectively, and the total cell numbers 1,742,800 and 1,808,800, respectively. 2. The ganglion cells based upon soma diameter were classified into 3 groups such as small (below 10㎛), medium (10-20㎛) and large (above 20㎛) ganglion cells. In 1-week-old duck, the relative frequencies of these 3 groups were 91.0%, 8.5% and 0.5%, respectively, and in 7-week-old duck, 83.7% 14.7% and 1.6%, respectively. 3. Area centralis was located at the distance of 1.6mm (1-week-old) and 2.0mm(7-week-old) nasal side from the anterior tip of the pecten. The number of ganglion cells in the area centralis was 11,000 cells/㎟ (1-week-old) and 12,000 cells/㎟ (7-week-old). 4. There was a gradual decrease in density of retinal ganglion cells extending from area centralis to the periphery of retina. 5. The density of the large (above 20㎛) ganglion cells was higher in the peripheral regions than in the center of retina. Higher densities were noted in the superior-temporal region in 7-week-old duck, and in the nasal region in 1-week-old duck than in the other regions. 6. The direction of the beak tip is approximately 30° in 1-week-old and 26° in 7-week-old duck from the angle of the pecten.

馬蹄鐵腎의 一例

金弘善,金武剛,趙聲煥,柳時潤 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

A case of horseshoe kidney which was found during dissection of cadaver was reported with a brief review of literatures. The main findings were as follows; 1. This horseshoe kidney was supplied by the 4 renal arteries were obtained from the aorta and 1 renal artery was originated from the common iliac artery. 2. The pelvis of the horseshoe kidney were placed anteriorly and the ureters were passed anteriolateral surface to the renal isthmus. 3. In the horseshoe kidney, the renal stone was not observed and the isthmus was consisted of the renal parenchyma.

혈관외피종 -1증례 보고-

강유미,지미경,양기화,강석진,김병기,김선무,정수일 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.24 No.2

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

혈관외피종 -1증례 보고-

강유미,지미경,양기화,강석진,김병기,김선무,정수일 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.25 No.5

각종 동물 설유두의 형태학적인 관찰

김홍선,김무강,김원식,이영호,신태균 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The lingual papillae of the mouse, the rabbit, and the cat were observed by light microscope and scanning electron microscope The results were followings 1. The filiform papillae of the mouse were classified to typical filiform papillae, conical filiform papillae with 2 branched tips, and general conical filiform papillae. The outmost layers of the filiform papillae of the mouse keratinted well, middle layer of the anterior portion of the papillae appeaered a lot of small basophilic granules. The fungiform papillae existed between the filiform papillae with the peripheral folds of epithelia. A vallate papilla existed on the posterior portion of conical filiform papillae and their shape was lenticuiar. 2. The filiform papillae of the rabbit revealed various type (5 types). Keratin layer of the papillae existed vertical to long axis of the papillae on the posterior portion of the papillae. Stratified squamous epithelium on the anterior portion of the papillae existed with a few keratin layers and the intercellular materials of strong PAS positive reaction appeared between the these cells. The fungiform papillae of the rabbit existed between the filiform papillae and had a few peripheral folds. The foliate papillae of the rabbit existed on the lateral portion of tongue root, consisted of the paralleled folds. Taste buds located in the mid-lateral portion of the papillae concentrically. 3. The filiform papillae on the anterior portion of the cat tongue shaped anterior and posterior projection, the filiform palillae on the middle portion had a projections of columnar shape, which papillae surface appeared as onion surface. The conical filiform papillae on the posterior portion had many folds on the lower portion of the papillae. The fungiform papillae of the cat existed between the filiforr papillae with a columnar shaped projectionand proturded from the surface. The vallate papillae of the cat existed between the conical filiform papillae and their shape was typical form of vallate papillae.

Rat의 복강에 삽입한 Fluorouracil-Polyglycolic acid 제형의 Fluorouracil 용출에 관한 연구

노승무,정경수,오정연,김진향,양준묵,강대영,송규상,최정목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 忠南大學校 癌共同硏究所 1998 癌共同硏究所 硏究誌 Vol.2 No.1

A common form of relapse in adenocarcinoma of the stomach is intraperitoneal dissemination, in fact, among gastric adenocarcinoma patients who have undergone surgery intended to cure, approximately 50% of the patients develope initial recurrence in the peritoneal cavity regardless of the anatomic site of the primary tumor within the stomach. The efficacy of systemic postoperative chemotherapy to prevent peritoneal recurrence of gastrric adcnocarcinoma is not satisfactory. There is still a great need for improved therapeutic strategies on the disseminated microscopic disease and small miliary nodules remaining on the peritoneal surface or lymphatics after operation. The authers have made fluorouracil-polyglycolic acid composite disks(Fu-PGA disks) with fluorouracil and biodegradable polymer: polyglycolic acid for more effective intraperitoneal chemotherapy. We inserted the Fu-PGA disk(s) in the peritoneal cavity of rat and pharmacokinetic study was performed to measure fluorouracil concentration in the peritoneal fluid, plasma, liver, kidney and heart tissue at 24 hour, 72 hour and 168 hour after insertion of Fu-PGA disk(s). Myelosuppressive action of this composite also was determined following its administration. The data of this study suggested that Fu-PGA composite will be a new device releasing drugs in a controlled manner and having targetability to peritoneum, and this device will be improving the efficacy of intraperitoneal chemotherapy for gastric adenocarcinoma.

절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구

김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.

위암환자의 복강내에 투여한 Mitomycin C-Carbon Particle의 Mitomycin 용출에 관한 연구

노승무,조영훈,정경수,오정연,김진향,양준묵,강대영,송규상,조준식,최선웅,이진호,민병무,김용백,김창식,박근성,인현빈,정현용,김학용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

Locoregional recurrence is the most common type of recurrence in surgical operation of gastric adenocarcinoma, and peritoneal dissemination is one of the most difficult problems in advanced gastric adenocarcinoma treatment. Because the peritoneal cavity is the most common site of the first recurrence after gastric cancer resection, intraperitoneal chemotherpy seems a logical choice for cancer chemotherapy. The Mitomycin C(MMC) adsorbed by the activated charcoal particles(CH) is relatively released when the drug concentration surrounding the carbon particles becomes low in the peritoneum of the peritoneal cavity. For the intraperitoneal chemotherapy on the advanced gastric adenocarcnoma, mitomycin C adsorbed on activated carbon particles was administered in the peritoneal cavity just before abdominal wall closure. The closed drainage tubes were inserted in the peritoneal cavity and clamped for tuo hours after completion of operation. MMC concentrations were serially measured in peritoneal fluid, plasma and urine at 2hour, 48 hour, 72 hour and 168 hour following its administration in order to study the efficacy of the MMC-CH as a drug delivery system. There were minimal toxicities in born marrow, liver, and gastrointestinal system after intraperitoneal MMC-CH administration. The data of this study suggested that MMC-CH may have a somewhat more beneficial effect than surgery alone when administered in optimal dose and schedules, but the MMC concentration of the peritoneal fluid was not sufficient to eradicate remnant cancer cells, and effective duration of maintenance was only below 24 hours in the peritoneal fluid and plasma.