Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Group Size And Measure Of Group-Level Properties

Kim,Kyoung-Su,Kim In-Sook,Jun,Gyung-Ju,Yang,Dong-Min 한국인적자원관리학회 2009 인적자원관리연구 Vol.16 No.4

Bliese와 Halverson(1998)은 집단수준의 효과를 분석하는데 있어서 기존에 사용되어 온 eta-squared 보다는 eta-squared 값이 사용되어야만 한다는 것을 주장하였다 그러나 이들이 제시한 수정된 eta-squared 접근은 다음과 같은 한계점을 가지게 되는 바 첫째, 이 접근법은 개인수준의 데이터를 집단 수준의 데이터로 만드는데 있어서의 정당성을 제공하는 기준을 구체적으로 제시하고 있지 못하다는 것을 들 수 있고, 둘째, 이 접근법은 또한 집단 내 효과를 용인하고 분석하는 기준을 제공하고 있지 못하다는 점을 들 수 있다 본 논문에서는 이러한 한계점을 극복할 수 있는 몇 가지의 대안을 제시하고 있으며, 이와 더불어 개인수준의 데이터를 집단 수준으로 만드는 것에 대한 정당성을 위한 또 하나의 추가적인 절차를 제시하였다 Bliese and Halverson(1998) argue that corrected eta-squared, rather than eta-squared, should be used for analyzing group-level effects However, their proposed corrected eta-squared approach has the following limitations (1) it fails to specify a criterion for justifying the aggregation of individual-level data to the group level and (2) it fails to specify a criterion for analyzing and allowing for within-group effects In this paper, we suggest several alternatives that overcome these limitations We suggest an additional procedure for justifying the aggregation of individual-level data to the group level

광주지역 소아에서 유행한 호흡기바이러스 유행양상 분석

서진종,김민지,김선희,기혜영,정재근,김은선,박종태,김경심,이수야,김명권,정윤석 대한감염학회 2008 감염과 화학요법 Vol.40 No.4

Background : This investigation was to perform the epidemiological surveillance and genetic analysis on respiratory viral agents from children with acute respiratory infections in Gwangju. Materials and Methods : For this study, 3,695 specimens obtained from patients with acute respiratory infections were collected by collaboration with pediatric hospitals in Gwangju between 2005 and 2007. Specimens were screened for 8 respiratory viruses including influenza viruses (IFV), human rhinoviruses (HRV), human coronaviruses (HCoV), adenoviruses (ADV), parainfluenza viruses (PIV), human enteroviruses (HEV), respiratory synthitial viruses (RSV) and human bocaviruses (HBoV). Respiratory viruses were detected using multiplex (RT) PCR with viral specific primers. Results : Out of 3,695 specimens, the ratio of virus detection was 24.9% (919). Overall, HRV (35.5%) and IFV (34.9%) were the most common viruses found, followed by HBoV (14.8%), HCoV (10.6%), RSV (3.7%), ADV (3.4%), PIV (3.2%) and HEV (3.0%). In addition, multiple infections were detected in 80 patients (8.7%). When the prevalence was analyzed according to season, HBoV, IFV and HCoV showed two epidemic points in late spring and early winter. ADV, HRV, RSV PIV and HEV, however, were all found to have only one epidemic point, with RSV being most common during winter and the others being most prominent during spring. Conclusions : Through this epidemiological surveillance, the respiratory viruses prevalent in children in Gwanaju area were investigated. We strongly recommend the development of nationwide policy for the management of prevalent respiratory virus that includes long term collection of data and samples, vaccine development and prevention education of the misuse of antibiotics.

건축공사시방서에서의 KS규격의 국제인증방안에 관한 연구

김경식,이재섭,김용수,이규진,조용훈,최완철,전재열,이현수,구재동,이기환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The purpose of this research is make out construction specifications for International Construction. Using off-shore products incur cost increase and longer delivery term of construction. and inconvenient on International Construction. Expand the range of additional local products conforming to performance. Life Cycle Cost. maintainability. operability. user's acceptability, etc.. And this study is suggest the standard formats and the techniques for construction specification.

남자 대학생의 음주 형태 변화에 영향을 미치는 유전적, 심리사회적 요인

김시경,이수경,김민경,이상익 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.4

Objectives : This study aimed to determine the general predictive factors of change in drinking behavior and to provide materials for preventing drinking problems during early adulthood through examining genetic and psychosocial factors affecting the change of drinldng behavior in college students. Methods : The subjects were 101 male college students, apart of 534 students who had completed the previous study in 2000. In the present study as a 6-years follow up, we reassessed the drinking pattem and psychosocial variables and compared the results with previous data of the same subjects. To identify factors affecting the current drinking pattem, we used stepwise multiple regession and logistic regression analysis. Results : D allele (ALDH2) was found to reduce the degree of drinking and suppress problematic drinking, and C allele (TPH) had a suppression effect on problematic drinking. Drinking motive had a direct effect on the degree of drinking and Pro-blematic drinking. Negative cognitive expectancy had a direct effect on problematic drinking. Conelusion : Authors found some factors affecting the change of alcohol drinking behavior in college students and confirmed that there were hierarchies of significance among these factors. These may be applicable as variables for predicting drinking behavior in early adulthood.

금속 촉매를 이용한 poly-Si의 결정화에 관한 연구

金善浩,李秀京,金泰延,康娜榮,金亨駿 弘益大學校 科學技術硏究所 2005 科學技術硏究論文集 Vol.16 No.-

Nickel induced crystallization of amorphous silicon (a-Si) has been studied by selective deposition of Ni on a-Si thin films. The kinetics of crystallization for MIC was found to be greatly enhanced than that of SPC. Ni-induced crystallization is a promising technique for low-temperature fabrication of poly-Si thin-film transistors needed for large area applications. Lateral growth of polycrystalline silicon mediated by the formation of nickel silicide, has been successfully employed for the fabrication of high-performance TFTs. A-Si thin films on which Ni films were deposited with various thickness were crystallized as a function of annealing temperatures. Throughout the thesis work, wise choice of both the thickness of Ni and the annealing temperature was found to produce poly-Si films with high quality, thus potentially resulting in the production of TFTs with high performance.

자가이식을 이용한 상악 매복 견치의 치료 : OF IMPACTED MAXILLARY CANINES

김수경,백병주,김재곤,양연미 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

부산지역 IT기업의 특성이 수출성과에 미치는 영향에 관한 연구

김미정,박경은,이춘수 한국관세학회 2009 관세학회지 Vol.10 No.1

According to the empirical results, it was found out that the R&D capability of a company's characteristics, overseas market experience and export immersion from the CEO's characteristics directly affected the qualitative export results. This study also found out that the R&D capability of a company's characteristics, export immersion from the CEO's characteristics and government overseas marketing support policy characteristics directly affected the qualitative export results. Therefore, a high R&D capability played a key role in quick market entrance and performance creation of technology oriented IT companies that have less market experience and insufficient business resources.

수도권 소재 산부인과의원의 제왕절개율과 관련 요인분석

김윤미,고수경 여성건강간호학회 2002 여성건강간호학회지 Vol.8 No.3

This study aims to find factors that affect variations in cesarean section frequency rates among OBGY clinics in Metropolitan areas. The factors include patient. medical supplier characteristics and economic factors. This study is a cross-sectional analysis using health insurance delivery claims from July to December 2000 and files of the NHIC(national health insurance corporation). Multiple regression was used to analyze the dependent variable of cesarean section frequency rate at each clinic. The results are as follows' Cesarean section frequency rate is increasing in proportion to the number of the following patients repeated caesarean section. disproportion. obstructed labour, fetal distress. emergency caesarean section and self-employed patients. There are geographic variations as well. Cesarean section frequency rates are higher in Inchon and Gyonggi province than in Seoul. The higher number of total delivery the clinic has. the lower rate of cesarean section it has. Clinics with high frequency rates in 1999 showed higher rates the next year. Further research is required to develop evidence based delivery modes and change strategies for increasing normal delivery and activating midwife clinics.

스키장의 서비스스케이프가 고객만족과 행동의도에 미치는 영향

김형섭,장경수,이귀옥 대한경영학회 2003 大韓經營學會誌 Vol.16 No.6

The purpose of this study is to develop scale items to measure servicescape of ski resort, to measure servicescape of ski resort to use them, and to find out relationships among servicescape, customer satisfaction and Behavioral intentions. Hypotheses were developed from reviewing the existing literature and tested empirically. The survey, based on questionnaire, was conducted in ski resort. The data were analyzed using SPSS 10.0 and structural equation model by LISREL 8.51. As a result of factor analysis on servicescape of ski resort, 6 factors were extracted and titled "lift", "ski slope", "facilities layout", "safety facilities", "other facilities", and "ski equipments". The results of the study show that servicescape of ski resort has a positive relationship with customer satisfaction, but has not relationship with behavioral intention. The customer satisfaction has a positive influence on behavioral intentions.