Complete genome sequence of Lactiplantibacillus plantarum ST, a potential probiotic strain with antibacterial properties

( Shujuan Yang ),( Chenglin Deng ),( Yao Li ),( Weicheng Li ),( Qiong Wu ),( Zhihong Sun ),( Zhenhui Cao ),( Qiuye Lin ) 한국축산학회 2022 한국축산학회지 Vol.64 No.1

Lactiplantibacillus plantarum (L. plantarum) ST was isolated from De’ang pickled tea in Yunnan Province, China. The genomes of strain ST were fully sequenced and analyzed using the PacBio RS II sequencing system. Our previous study has shown that L. plantarum ST is a potential probiotic strain. It had strong tolerance in the simulated artificial gastrointestinal tract, and in the antagonism tests, this strain showed strong antibacterial activity. Therefore, as a probiotic, it may be used in animal breeding. L. plantarum ST genome was composed of 1 circular chromosome and 7 plasmids. The length of the whole genome was 3320817 bp, and the annular chromosome size was 3058984 bp, guanine + cytosine (G ± C) content (%) was 44.76%, which contained 2945 protein-coding sequences (CDS). This study will contribute to a further comprehensive understanding of L. Plantarum ST at the genomic level and provide a theoretical basis for its future application in animal breeding.

Micromechanical modeling for viscoplastic properties of enzyme degradable semi-crystalline poly (ε-caprolactone)

Xinwei Yang,Cheng Yang,Shujuan Hou,Yiqi Mao 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.11

This work provide theoretical understandings for the enzyme-degradable PCL, and assist its structural designs and engineering applications. An energy-dependent evolution model is developed to reflect the enzyme-triggered decrystallization of crystals and the further dissolution by applying a chain-broken chemical reaction. Then, the mechanical properties of the enzyme-degradable semicrystalline PCL is modelled through the homogenization-based procedure by the volume-average of a collection of laminated inclusions with crystals and amorphous phase. A dual-phase-lag diffusion model is advanced to solve the enzyme concentrations in the PCL. The model is calibrated by the experiments and then applied for the chemomechanical properties of the PCL when under enzyme conditions. Some numerical examples are conducted to discuss effects of the enzyme concentration and the crystallinity on the crystallographic axe evolution as well as the overall chemomechanical properties of the semicrystalline PCL.

Five new species of Stenothemus Bourgeois from Guangxi, China (Coleoptera, Cantharidae)

Ge Shujuan,Liu Haoyu,Yang Xing-ke,Yang Yuxia 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Five new species of Stenothemus Bourgeois, 1907 from Guangxi, China are described, S. normalis sp. nov., S. flavicans sp. nov., S. nigripennis sp. nov., S. elongaticeps sp. nov. and S. maculaticollis sp. nov.. They are illus trated with habitus photos and aedeagi of males, also abdominal sternites VIII and internal genitalia of females. A key for the identification of the above species is provided.

Massive MIMO TWO-Hop Relay Systems Over Rician Fading Channels

( Jian Cao ),( Shujuan Yu ),( Jie Yang ),( Yun Zhang ),( Shengmei Zhao ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.11

With the advent of the fifth-generation (5G) era, Massive multiple-input multiple-output (MIMO) relay systems have experienced the rapid development. Recently, the performance analysis models of Massive MIMO relay systems have been proposed, which are mostly based on Rayleigh fading channels. In order to create a more suitable model for 5G Internet of Things scenarios, our study is based on the Rician fading channels, where line-of-sight (LOS) path exists in the channels. In this paper, we assume the channel state information (CSI) is perfect. In this case, we use statistical information to derive the analytical exact closed-form expression for the achievable sum rate of the uplink for the Massive MIMO two-hop relay system over Rician fading channels. Moreover, considering the different communication scenarios, we derive the analytical exact closed-form expression for the achievable sum rates of the uplink for other three scenarios. Finally, based on these expressions, we make simulations and analyze the performance under different transmit powers and Rician-factors, which provides a theoretical basis and reference for further research.

Gene microarray analysis revealed a potential crucial gene RACK1 in oral squamous cell carcinoma (OSCC)

Jian-Wei Zheng,Yinshen Yang,Shujuan Yang,Wei Zhou,Hongtian Qiu,Xiaoping Li,Qiuyun Cai,Ting Li,Gang Luo 한국통합생물학회 2018 Animal cells and systems Vol.22 No.2

Oral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide, which appears as a consequence of multiple molecular genetic events in various chromosomes and genes. In order to unveil the possible mechanisms underlying OSCC tumorigenesis, the OSCC-related gene expression variance and the gene interaction network should be further investigated. Herein, we conducted the NimbleGen Human Gene Expression Microarray to analyze expression heterogeneity between OSCC primary tumor tissue and its adjacent normal tissue from two patients. A total number of 7872 out of 32,448 detected genes are differentially expressed in OSCC. Gene ontology (GO) analysis demonstrated that these differentially expressed transcripts were critical in a series of metabolic processes, cancer-related signal pathways, and biological regulations. KEGG signaling pathway enrichment suggested a number of pathways (metabolic process and immune response) which are frequently enrolled during cancer progression. 15 most differential regulated genes between OSCC tumor and non-tumor were confirmed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, the interaction network analysis of these confirmed genes by STRING database showed the two subunits of RACK1 had direct interaction with 14 differential proteins. This bioinformatics research lends support about the critical role of RACK1 which functions as a key node protein driving OSCC development.

Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate

Zhang, Shuang,Shang, Wenting,Yang, Xiaoxi,Zhang, Shujuan,Zhang, Xiaogang,Chen, Jiawei Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

The immobilization of enzyme is one of the key issues both in the field of enzymatic research and industrialization. In this work, we reported a facile method to immobilize Candida Antarctica lipase B (CALB) in alginate carrier. In the presence of calcium cation, the enzyme-alginate suspension could be cross-linked to form beads with porous structure at room temperature, and the enzyme CALB was dispersed in the beads. Activity of the enzyme-alginate composite was verified by enzymatic hydrolysis reaction of p-nitrophenol butyrate in aqueous phase. The effects of reaction parameters such as temperature, pH, embedding and lyophilized time on the reactive behavior were discussed. Reuse cycle experiments for the hydrolysis of p-nitrophenol butyrate demonstrated that activity of the enzyme-alginate composite was maintained without marked deactivation up to 6 repeated cycles.

Construction of DOPA-SAM multilayers with corrosion resistance via controlled molecular self-assembly

Ting Chen,Ming Yang,Hui Yang,Ruining Wang,Shujuan Wang,Hang Zhang,Xiaoyu Zhang,Zhijuan Zhao,Jinben Wang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.69 No.-

Although nano “green” coatings with excellent corrosion resistance have attracted great attention, the inhibition efficiency is still limited due to the lack of knowledge about the correlation between molecular structure and anticorrosion performance. Here, we fabricated a series of 3,4-dihydroxy-l-phenylalanine adlayers on self-assembled monolayers (SAMs) with varying end groups. We found that both NH2 and CF3SAMs were more conducive to the adsorption of DOPA and a flat adsorption conformation was preferentially adopted, with the plane of the phenylene ring parallel to the surface via cation-π interactions or hydrophobic interactions, leading to a compact and dense adlayer. Such DOPA-SAM multilayers can effectively protect the substrate from corrosion by suppressing the diffusion of aggressive water and acid molecules as well as the electrodissolution of metals. The lowest corrosion current of adlayers reaches 6.96 μA cm−2 which is much lower than that of bare substrate and other anticorrosion surfaces reported previously. The results provide guidance on the design of green anticorrosion materials via selecting SAMs that bridge organic and metal interface.

Metastatic pattern of ovarian cancer delineated by tracing the evolution of mitochondrial DNA mutations

Xu Zhiyang,Zhou Kaixiang,Wang Zhenni,Liu Yang,Wang Xingguo,Gao Tian,Xie Fanfan,Yuan Qing,Gu Xiwen,Liu Shujuan,Xing Jinliang 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Ovarian cancer (OC) is the most lethal gynecologic tumor and is characterized by a high rate of metastasis. Challenges in accurately delineating the metastatic pattern have greatly restricted the improvement of treatment in OC patients. An increasing number of studies have leveraged mitochondrial DNA (mtDNA) mutations as efficient lineage-tracing markers of tumor clonality. We applied multiregional sampling and high-depth mtDNA sequencing to determine the metastatic patterns in advanced-stage OC patients. Somatic mtDNA mutations were profiled from a total of 195 primary and 200 metastatic tumor tissue samples from 35 OC patients. Our results revealed remarkable sample-level and patient-level heterogeneity. In addition, distinct mtDNA mutational patterns were observed between primary and metastatic OC tissues. Further analysis identified the different mutational spectra between shared and private mutations among primary and metastatic OC tissues. Analysis of the clonality index calculated based on mtDNA mutations supported a monoclonal tumor origin in 14 of 16 patients with bilateral ovarian cancers. Notably, mtDNA-based spatial phylogenetic analysis revealed distinct patterns of OC metastasis, in which a linear metastatic pattern exhibited a low degree of mtDNA mutation heterogeneity and a short evolutionary distance, whereas a parallel metastatic pattern showed the opposite trend. Moreover, a mtDNA-based tumor evolutionary score (MTEs) related to different metastatic patterns was defined. Our data showed that patients with different MTESs responded differently to combined debulking surgery and chemotherapy. Finally, we observed that tumor-derived mtDNA mutations were more likely to be detected in ascitic fluid than in plasma samples. Our study presents an explicit view of the OC metastatic pattern, which sheds light on efficient treatment for OC patients.

Immobilization of Lipase using Alginate Hydrogel Beads and Enzymatic Evaluation in Hydrolysis of p-Nitrophenol Butyrate

Shuang Zhang,Wenting Shang,Xiaoxi Yang,Shujuan Zhang,Xiaogang Zhang,Jiawei Chen 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

The immobilization of enzyme is one of the key issues both in the field of enzymatic research and industrialization. In this work, we reported a facile method to immobilize Candida Antarctica lipase B (CALB) in alginate carrier. In the presence of calcium cation, the enzyme-alginate suspension could be cross-linked to form beads with porous structure at room temperature, and the enzyme CALB was dispersed in the beads. Activity of the enzyme-alginate composite was verified by enzymatic hydrolysis reaction of p-nitrophenol butyrate in aqueous phase. The effects of reaction parameters such as temperature, pH, embedding and lyophilized time on the reactive behavior were discussed. Reuse cycle experiments for the hydrolysis of pnitrophenol butyrate demonstrated that activity of the enzyme-alginate composite was maintained without marked deactivation up to 6 repeated cycles.

Biosynthesis of rare 20(R)-protopanaxadiol/protopanaxatriol type ginsenosides through Escherichia coli engineered with uridine diphosphate glycosyltransferase genes

Lu Yu,Yuan Chen,Jie Shi,Ru-Feng Wang,Ying-Bo Yang,Li Yang,Shujuan Zhao,Zheng-Tao Wang 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.1

Background: Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such as Asian ginseng, American ginseng, and Notoginseng. Some ginsenosides, especially the 20(R) isomers, are found in trace amounts in natural sources and are difficult to chemically synthesize. The present study provides an approach to produce such trace ginsenosides applying biotransformation through Escherichia coli modified with relevant genes. Methods: Seven uridine diphosphate glycosyltransferase (UGT) genes originating from Panax notoginseng, Medicago sativa, and Bacillus subtilis were synthesized or cloned and constructed into pETM6, an ePathBrick vector, which were then introduced into E. coli BL21star (DE3) separately. 20(R)-Protopanaxadiol (PPD), 20(R)-protopanaxatriol (PPT), and 20(R)-type ginsenosides were used as substrates for biotransformation with recombinant E. coli modified with those UGT genes. Results: E. coli engineered with GT95syn selectively transfers a glucose moiety to the C20 hydroxyl of 20(R)-PPD and 20(R)-PPT to produce 20(R)-CK and 20(R)-F1, respectively. GTK1- and GTC1-modified E. coli glycosylated the C3eOH of 20(R)-PPD to form 20(R)-Rh2. Moreover, E. coli containing p2GT95synK1, a recreated two-step glycosylation pathway via the ePathBrich, implemented the successive glycosylation at C20eOH and C3eOH of 20(R)-PPD and yielded 20(R)-F2 in the biotransformation broth. Conclusion: This study demonstrates that rare 20(R)-ginsenosides can be produced through E. coli engineered with UTG genes.