동일한 시료에 대한 국내 기관간의 STR 분석결과 비교 : STR 유전자좌 분석법의 표준화 설정을 위하여

박종태,신경진,양윤석,우광만,이숭덕,이승환,이정빈,정연보,조승희,한길로,한면수,홍승범 大韓法醫學會 2001 대한법의학회지 Vol.25 No.1

This paper described a collaborative exercise intended to see what kinds of short tandem repeat (STR) loci are used in different DNA typing laboratories in Korea and to compare their results for the demonstration whether uniformity of DNA profiling results from different laboratory could be achieved in Korea Laboratories were asked to test five tissue DNAs using methods routinely used in each laboratory and to report the results to the coordinating laboratory. The exercise demonstrated that each laboratory was using different STR loci for the typing with different STR numbers,2 VNTRS,36 STRs and amelogenin in total, and the direct comparison of the results from all the laboratory for the 18 loci could not be done as only one laboratory submitted typing results. Among 21 loci for which several laboratories submitted typing results, results for 14 loci were the same and results for the other 7 loci were different depending on the participating laboratory. D1S80, F13A01, D16S539, D21S11, D18S51, D3S1744 were the loci with different typing results. Even in the cases where commercial kits were used, the results were not the same depending on the machines used, that is the capillary electrophoresis or the gel based electrophoresis. The reason for the different results, points about the standardization of the methods arid the profiling data were described.

난소의 성국 낭성기형종에서 관찰된 전립선 조직 - 1예 보고 -

홍승모,최미선,한은미,이현경,노재윤 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.7

MeV이온주입을 사용하여 Buried Layer를 포함한 CMOS Retrograde Well 제조시 발생하는 격자 결함에 관한 연구

盧在相,柳承翰,柳漢權 弘益大學校 科學技術硏究所 1998 科學技術硏究論文集 Vol.9 No.2

MeV ion implantation has been recently employed in the field of CMOS (complementary metal oxide semiconductor) retrograde well engineering. An issue on MeV ion induced damage is critical especially in forming a buried layer below the well. MeV B implanted buried layers were observed to show greatly improved characteristics of latchup suppression. Junction leakage current, however, showed a critical behavior as a function of ion does. The rod like defects were observed to be responsible for the leakage current. Rod-like defects were generated near the ??(projected range) and grown upward to the surface during annealing. According to cross sectional examination of each pit density, they were generated and propagated between 700。C and 800。C. They shrink or change into long, elongated dislocation loops at higher temperatures above 900。C. Results of SIMS (secondary ion mass spectroscopy) analyses and two-step-annealing (700。C/3∼6hrs.→900。C/1hr.) indicate that interstitial oxygens impede shrinkage of existing-g rod-like defects at higher temperatures above 900。C.

자동제어 시스템의 안정화에 미치는 모타 설계 변수의 영향

노승훈,조한중 金烏工科大學校 1994 論文集 Vol.15 No.-

Servomotors are widely used for the modern control systems in various ways. In this thesis, the effects of the servo parameters are investigated to obtain the optimum design for the given situation. In order to analyze the effects of those parameters the result of the variation of the combined values as well as the individual parameters is considered. The simulation algorithm and the optimization process used in the study are readily applicable to many control systems.

2¼Cr-1Mo강 압력용기 용접이음부의 재열균열에 관한 역학적 연구

권영섭,노찬승,방한서 朝鮮大學校 機械技術硏究所 1999 機械技術硏究 Vol.2 No.2

구조물에 사용되는 각종 압력용기들의 재질은 고온 및 고압에 견딜 수 있도록 요구되고 있다. 따라서, 내열, 내압 및 내부식성이 뛰어난 2¼Cr-1Mo강의 수요가 향후 증대될 전망이다 용접잔류응력은 일반적으로 응접구조물에 각종 악영향을 미치는 것으로 알려져 있다. 이 때문에 용접잔류응력. 확산성 수소제거 및 용접 열영향부의 연화등을 목적으로 용접후열처리(PWHT)를 수행하나, PWHT를 행하면 사용 강재의 종류에 따라 재열균열이 발생하고 있다. 본 연구에서는 2¼Cr-1Mo강에 대하여 실험과 수치해석 기법을 통하여 용접이음부의 재열균열 감수성평가 및 상당크리프 변형율의 재열균열 발생에 판정에 대한 역학적 지표로서의 적용성을 검토하였다. 2¼Cr-1Mo steels are widely used to fabricate pressure vessels. Residual stresses, in general, are known to be harmful to the soundness of the welded structures. PWHT is release residual stresses, which may cause cracks. But, in those PWHT process, 2¼Cr-1Mo steels are apt to occur reheat crack frequently. In this reason, it is strongly needed to analysis and examine the mechanical behavior of welded joints through welding to PWHT process. So, in this study, welded nozzle parts of pressure vessel where reheat cracks are frequently occur are selected for examine the crack-occurrence mechanism.

돼지난소에서 난포폐쇄와 큰포식세포에 관한 형태학적 연구

김원식,한승로,김수일,박창식 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

난포폐쇄가 과립층세포의 세포자멸사에 의해 이루어지고 이 과정에 큰포식세포가 직? 간접적으로 관여한다는 사실은 널리 알려져 있다. 그러나 난포내에서 일어나는 세포자멸사가 과립층의 어디에서부터 개시되고 어떻게 파급되는지, 세포자멸사 산물과 퇴화된 난모세포의 제거 방법 그리고 난포폐쇄의 완성은 어떻게 이루어지는가에 대해서는 아직 확실히 밝혀져 있지 않다. 이에 저자들은 가임기 돼지(Yorkshire-breed)를 실험동물로 난소내 난포의 광학현미경적 및 투과전자현미경적 관찰과 돼지 대식세포 단크곤항체 4E9를 이용한 변역조직화학적 방법으로 본 연구를 실시하였다. 광학현비경적관찰 결과, 난포폐쇄는 과립층세포의 세포자멸사로부터 개시되고 이어 난모세포의 퇴화와 난포막 속층 세포들의 세포자멸사가 일어나면서 난포폐쇄가 완성되는 것으로 보인다. 과립층세포의 세포자멸사는 핵농축으로부터 시작되고 과립층의 깊이에 관계없이 짧은 시간 안에 모든 층으로 파급되고 최종적으로 난모세포의 투명대를 둘러싸고 있는 과립층세포의 세포자멸사포 이어지는 것으로 보인다. 투과전자현미경 관찰에서는 세포자멸사중에 있는 과립층세포들이 인접한 정상적인 과립층세포들과 큰포식세포들에 의해 포식되는 것을 확인할 수 있었고, 난포동내에 산재한 세포자멸사 소체들의 제거에도 큰포식세포들이 관여하고 있음을 알 수 있었다. 난포폐쇄와 큰포식세포와의 관계를 알아보기 위해 실시한 면역조직화학적 관찰에서는, 과립층세포의 세포자멸사 초기에는 큰포식세포가 과립층내에서만 발견되었으나, 세포자멸사의 진행과 함께 난포동과 난모세포를 둘러싸고 있는 투명대 주위에서 각각 순서대로 관찰되었고 마지막으로 난포막 속층에서도 발견되어, 이들이 과립층세포들의 세포자멸사 소체들을 제거하는 것은 물론 최종적으로는 난포막 속층 세포들의 세포자멸사 소체들을 제거하여 난소 실질화에 관여하는 것으로 보인다. 이러한 본 연구의 결과는 난포폐쇄와 관련된 큰포식세포의 구조, 기능적 연구에 유용한 자료로 생각된다. Apoptosis of granulosa cells leads follicular atresia and macrophages have an important role during the apoptotic process. However, the propagation of apoptosis within the follicle, the ways of elimination of apoptotic bodies and degenerated oocyte, and the completion of follicular atresia are still controversial and unidentified clearly. Using adult porcine (Yorkshire-breed) ovary, in this morphological study, transmission electron microscopic observation and immunohistochemical study with pig macrophage monoclonal antibody 4E9 were performed. In light microscopy, the follicular atresia initiated with apoptosis of granulosa cells, followed by degeneration of oocyte and apoptosis of theca interna cells. Apoptosis occured in random fashion among the granulosa cells and propagated multidirectionally, and finally to the granulosa cells surrounding zona pellucida of degenerating oocyte. Pyknosis of granulosa cells was the first sign of apoptosis. In immunohistochemistry, macrophages were found only in the granulosa layer at the stage of beginning of apoptosis. With progression of apoptosis, they were proliferated greatly in number enough to eliminate all the apoptotic bodies, and found within the follicular antrum. In advanced stage of atresia, macrophages surrounded the zona pellucida of degenerating oocyte, and found also in the theca interna. In transmission electron microscopy, phagocytic granulosa cells maintained characteristic gap junctions with neighboring granulosa cells and contained several apoptotic bodies and lipid droplets within their cytoplasm. Macrophages kept many apoptotic bodies, vacuoles and autophagosomes in their cytoplasm. Apoptotic granulosa cells were ingested by intact granulosa cells and macrophages initially, but lately, all the apoptotic granulosa cells and degenerated oocyte were eliminated by macrophages. Ovarian follicular atresia completed with phagocytosis of apoptotic theca interna cells by macrophages, and the remnants of the atretic follicle became ovarian stroma. It is well known that macrophages may play an important role during follicular atresia, such as elimination of apoptotic granulosa cells, theca intema cells and degenerated oocytes, but, the valid action mechanisms of macrophages on the initiation of granulosa cell apoptosis and on the completion of atresia through the secretion of paracrine factors and autocrine factors still unclear.

5-FU로 백서 가슴샘에서 유도된 세포고사의 Comet Assay에 의한 분석

김수원,한승로,정옥,조근자,김선,김원식 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Thymus is a lymphoid organ forming T-cells from hematogenous stem cells. Apoptosis of thymocytes in the cortex of thymus is so rapid that cannot find easily with routine histological techniques. In this experiment, Comet assay and SDS-PAGE were used to detect apoptotic changes and protein changes after 5-fluorouracil(5-FU) treatment in the thymus of the rat. The results were as follows. 1. The number of thymocytes in the cortex was markedly decreased, and many apoptotic cells with various size and shapes were found on DAT 5 in 5-FU treated group. 2. On DAT 5, new nodules with germinal centers began to appear in the peripheral cortex, and in the germinal center, there was many apoptotic bodies of various size and shapes. 3. In SDS-PAGE, a protein with molecular weight of 123kDa was increased on DAT 5 which was considered as integrins subfamily.

카드뮴에 의해 유발된 흰쥐 고환의 미세구조 및 단백질 변화

황일면,한승로,정옥,김수일,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 2000 충남의대잡지 Vol.27 No.2

Although cadmium is a well known heavy metal which attacks testis and results male infertility, the mechanism of action in the testis is'nt still fully known. In the previous experiment, authors observed morphological changes in the testis, and found cadmium-induced death of all the spermatogenic cells and hemorrhagic necrosis within the seminiferous tubules, and apoptosis and death of interstitial cells and fibroblasts in the interstitial tissue of the testis. In this experiment, cadmium chloride (4 mg/kg of body weight) administered to the adult male rats(Sprague-Dawlcy) intraperitoneally and sacrificed 1 week after treatment. And electrophoresis, immunoprecipitation and Western blotting, and N-terminal analysis were performed to find the proteins changed after cadmium treatment in the testis. And the results were as follows; 1. Two proteins of molecular weight 42kDa and 21kDa disappeared after cadmium treatment. 2. The protein of 2lkDa was rat collagen type I alpha 2. According to the above results, it was considered that cadmium degraded the collagen fibers within the wall of small blood vessels in seminiferous tubules and interstitial tissues and disrupted vascular walls, which resulted hemorrhagic necrosis, death of all the spermatogenic cells, and the death of interstitial cells and fibroblastic cells.

방사선 조사에 의한 흰주 난포의 퇴축과 난포세포의 방사선 감수성

김수일,한승로,조근자,허대영,이영호,조문준,김무강,김원식 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

The aim of this study were to investigate radiation-induced atresia and radiation susceptibility in the rat ovary morphologically. Female rats (Sprague Dawley strain) of 4 weeks old were irradiated with dose of 4 Gy and 8 Gy, and sacrificed at hour 6, 12, and 24 after radiation. The H & E stain, the TUNEL method (ApopTag kit) and transmission electron microscopy (TEM) were used. In light microscopic observation, the number of atresia of ovarian follicles were increased significantly at 6h after irradiation (p<0.01). There was no significant difference between 4 Gy and 8 Gy irradiated groups. In TEM observation, granulosa cells in radio-sensitive follicles were characterized by several structural features including condensation of nuclear chromatin granules, nuclear fragmentation and apoptotic body formation. An apoptotic cell is observed to have been phagocytosed by a normal granulosa cell. Granulosa cells in radio-resistant follicles were characterized by several structural features including nuclear indentation, partial condensation of chromatin granules, mitochondrial swelling, dilatation of RER cisterns, accumulation of dense irregular masses, accumulation of lipid droplets, and increased lysosomal bodies. Number of gap junctions between granulosa cells were decreased, and intercellular space were widen than that of control animals. These findings were prominent at 6h after irradiation and were diminished at 12h and 24h after irradiation. With these results, it was concluded that radiation-induced follicular cell apoptosis and ovarian follicular atresia in rat ovary increased considerably at 6h after irradiation. Further studies are needed to reveal the more extensive differences between radiosensitive and radioresistant follicular granulosa cells.

흰쥐 난소에서 황체내 큰포식세포에 관한 형태학적 연구 : 면역조직화학적 및 투과전자현미경적 연구

김원식,한승로,조근자,김수일,박창식 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

황체는 임신의 수립과 유지에 필수적인 일시적인 내분비샘으로, 주기능을 발정주기의 생리적 기간을 결정하고 성공적인 임신을 달성하는데 결정적인 프로게스테론의 생산이다. 황체의 기능은 뇌하수체 gonadotropin 뿐만 아니라 황체내 큰포식세포에서 분비되는 TNF-α를 포함한 몇가지 cytokines에 의해 조절되는 것으로 알려져 있다. 본 연구에서는 흰쥐 (Sprague-Dawley계통)에서 생식주기별 황체내 큰포식세포의 변화를 면역조직화학적 및 투과전자현미경적으로 관찰 비교하였다. 본 연구에서 흰쥐 황체에서 큰포식세포는 배란기,임신기 및 출산후기 등 모든 시기에 나타나고,그 수와 면역반응성은 배란기에 가장 컸고 출산후기,임신기 순으로 나타났다. 아울러 투과전자현미경 관찰에서는 황체내 큰포식세포를 세포체가 가늘고 돌기가 긴 비포식성 큰포식세포와 포식성 공포와 지방소체들을 많이 함유한 포식성 콘포식세포로 분류할 수 있었다. 본 연구를 통해 황체내 콘포식세포는 발정주기에 따라 그 수나 면역반응성이 다르며,황체퇴화가 활발히 진행되는 배란기와 출산후기에 면역반응성이 증가하고 포식성 큰식세포들이 나타난 것은 큰포식세포에서 TNF-α 와 같은 cytokines의 분비를 활발히 일으키고 그들이 황체세포들의 세포자멸사를 유발,촉진하고 또한 세포자멸 사소체들을 포식,제거하는 방법으로 황체퇴화를 유도하여 정상적인 발정주기를 유지하기 위한 기전으로 생각 되고,임신기에 큰 포식세포의 수와 면역반응성이 작아지고 비포식성 큰포식세포가 주를 이루는 것은 이들이 TNF-α 동의 cytokines 분비를 통해 그들이 황체자극효과를 나타내 황체의 기능을 유지,촉진하는데만 관여하고,한편으로는 황체에서 생산되는 프로게스테론에 의한 큰포식세포 억제작용 때문인 것으로 생각된다. Macrophages in the corpus luteum have many important roles during the periods of functional development and luteal regression. Not only phagocyte the apoptotic luteal cells, but also they secrete many cytokines and exert their effects via autocrine/paracrine actions. In this study, we investigated the changes of number and immunoreactivity of macrophages at various developmental periods of the corpus luteum in the rat ovary. The rats (Sprague- Dawley strain, female) at age of 8 weeks (ovulatory period), GD 6 (pregnant period), and postpartum 5 days (postpartum period) were sacrificed under ether anesthesia and obtained both ovaries, one used for macrophages immunohistochemistry and the other used for TEM. The results were as follows; I. In the corpora lutea of the rat, macrophages were observed all the developmental periods including ovulatory, pregnant and postpartum periods. 2. In the corpora lutea of the rat, number of macrophages was highest in the ovulatory period, and decreased at postpartum period and pregnant period in order. The immunoreactivity of macrophages was high at ovulatory period, moderate at postpartum period, and low at pregnant period. 3. In TEM observations, two types of macro phages were observed: One type was non-phagocytic macrophage and the other type was phagocytic macrophage. Phagocytic macrophages were observed in the corpora lutea at ovulatory and postpartum periods and contained apoptotic bodies, phagocytic vacuoles and many lipid droplets. Non-phagocytic macrophages were observed in the corpora lutea at pregnancy period and showed slender cell body with long cytoplasmic processes and contained no apoptotic bodies. In the rat, the number and the degree of immunoreactivity of macrophages in the corpus luteum varied with the changes of functional state of the corpus luteum. It was suggested that the main function of the macrophages at the ovulatory and postpartum periods was elimination of apoptotic luteal cells and that at pregnancy period was autocrine/paracrine action. Ultrastructurally, two types, phagocytic and nonphagocytic types, of macrophages confirmed. These results will provide valuable informations on the study of the role macrophages during development and regression of corpus luteum.