In vivo transduction of ETV2 improves cardiac function and induces vascular regeneration following myocardial infarction

Lee, Sunghun,Lee, Dong Hun,Park, Bong-Woo,Kim, Riyoun,Hoang, Anh Duc,Woo, Sang-Keun,Xiong, Wenjun,Lee, Yong Jin,Ban, Kiwon,Park, Hun-Jun Nature Publishing Group UK 2019 Experimental and molecular medicine Vol.51 No.2

<▼1><P>Vascular regeneration in ischemic hearts has been considered a target for new therapeutic strategies. It has been reported that ETV2 is essential for vascular development, injury-induced neovascularization and direct cell reprogramming of non-endothelial cells into endothelial cells. Thus, the objective of this study was to explore the therapeutic potential of ETV2 in murine models of myocardial infarction in vivo. Direct myocardial delivery of lentiviral ETV2 into rodents undergoing myocardial infarction dramatically upregulated the expression of markers for angiogenesis as well as anti-fibrosis and anti-inflammatory factors in vivo. Consistent with these findings, echocardiography showed significantly improved cardiac function in hearts with induced myocardial infarction upon ETV2 injection compared to that in the control virus-injected group as determined by enhanced ejection fraction and fractional shortening. In addition, ETV2-injected hearts were protected against massive fibrosis with a remarkable increase in capillary density. Interestingly, major fractions of capillaries were stained positive for ETV2. In addition, ECs infected with ETV2 showed enhanced proliferation, suggesting a direct role of ETV2 in vascular regeneration in diseased hearts. Furthermore, culture media from ETV2-overexpressing cardiac fibroblasts promoted endothelial cell migration based on scratch assay. Importantly, intramyocardial injection of the adeno-associated virus form of ETV2 into rat hearts with induced myocardial infarction designed for clinical applicability consistently resulted in significant augmentation of cardiac function. We provide compelling evidence that ETV2 has a robust effect on vascular regeneration and enhanced cardiac repair after myocardial infarction, highlighting a potential therapeutic function of ETV2 as an efficient means to treat failing hearts.</P></▼1><▼2><P><B>Cardiovascular disease: New hope for healing the heart</B></P><P>A gene therapy strategy that stimulates cardiovascular repair could improve recovery for heart attack patients. Heart attacks inflict severe damage on the heart and blood vessels, tissues with limited capacity for self-repair. Researchers led by Kiwon Ban of the City University of Hong Kong and Hun-Jun Park of the Catholic University of Korea, Seoul, have now demonstrated that a gene responsible for cardiovascular development can also efficiently stimulate heart repair. They used viruses to deliver the gene into a mouse model of heart attack, and showed that treated heart tissues exhibited strong recovery relative to untreated controls. The treatment reduced scar tissue formation and promoted proliferation of the cells lining blood vessels and blood vessel formation, measurably improving heart function. This approach could lay the groundwork for treating a common potentially fatal event.</P></▼2>

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

초등학생들의 비만·식이에 대한 지식 및 태도에 영향을 미치는 요인

우상훈,오경재 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

양안의 실명으로 발현한 수막암종증 1예

김성훈,고상배,이광우 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

ATM망의 VPC/VCC Connection Group 관리를 위한 Circular-Get-Request

윤영우,문성헌 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.2

Recently, along with many studies to improve the traditional SNMP, some plans to effectively apply SNMP to the management of ATM networks have been devised. Connection Group MIB is one of these plans. It is proposed as a method to manage the VPC/VCC of the ATM switches, that is to say, as a method to equalize the indexes of the identical managed objects among the switches. But, Considering current technology of multicast services on the ATM network, the benefits gained by application of Connection Group MIB method to the real ATM network, would not so great. In this paper, we analyze some problems of Connection Group MIB method and propose a new circular-get request PDU to solve these problems.

소아과 입원 환자에 대한 통계적 고찰

정용현,서우식,유재홍,변상현 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

For the purpose of assessing the disease patterns by the body organ system in patients of the pediatric age, we performed a statistical analysis of diseases of patients admitted to the department of pediatrics at Chungnam National University Hospital from January 1990 to December 1994. The results obtained were as follows : 1. Total numbers of in-patients were 8,007, of which 4,923 were male and 3,084 were female. The ratio male to female was 1.60 : 1. 2. Prevalence of the diseases classified by body organ system was in the following order : neonatal diseases (34.6%), respiratory diseases (17.4%), infectious diseases (11.5%), congenital anomalies (5.5%), digestive diseases (5.1%), genitourinary diseases (4.9%), neoplasm (4.7%), blood diseases (3.3%), nervous system diseases (2.8%), metabolic and endocrine diseases (2.4%), circulatory system diseases (2.3%), skin and subcutaneous diseases (1.3%), musculoskeletal and connective tissue diseases (1.2%), mental and behavior disorders (0.4%). 3. Major diseases in each body organ system were as follows : 1) infectious diseases : unspecified acute gastroenteritis (35.9%), viral meningitis (10.4%), Rotaviral enteritis (6.8%), sepsis (6.7%), measles (5.8%). 2) neoplasm ; ALL (34.1%), AML (15.8%), Wilms tumor (12.8%), NHL (8.2%), neuroblastoma (5.6%) 3) blood diseases : allergic purpura (24.2%), aplastic anemia (17.1%), ITP (16.4%), iron deficiency anemia (15.8%). 4) metabolic and endocrine diseases : short stature (22.0%), hypocalcemia (13.8%), metabolic acidosis (9.6%), IDDM (6.4%). 5) mental and behavior diseases : neurosis (47.7%), mental retardation (18.2%). 6) Nervous and sensory system diseases : epilepsy (43.2%), cerebral palsy (12.1%), otitis media (9.3%), encephalopathy (6.2%). 7) circulatory system diseases: congestive heart failure (29.2%), dilated cardiomyopathy (8.0%), rheumatic fever (8.0%), mitral insufficiency (6.8%), hypertension (6.4%). 8) respiratory diseases : pneumonia (40.1%), URI (9.6%), acute tonsillitis (9.4%), asthma (8.5%), acute bronchiolitis (8.3%), croup (7.1%), acute bronchitis (4.1%). 9) digestive system diseases : unspecified hepatitis (22.8%), acute gastritis (13.8%). CAH (12.6%), intussusception (7.8%), constipation (7.1%). 10) skin and subcutaneous diseases : cellulitis (23.1%), lymphadenitis (19.7%), urticaria (15.6%), atopic dermatitis (8.8%), furuncle (8.2%). 11) musculoskeletal and connective tissue diseases : Kawasaki disease (76.8%), JRA (8.5%), SLE (7.0%). 12) genitourinary system diseases : UTI (29.0%), minimal change nephrotic syndrome (27.3%), APSGN (7.6%), APN (4.9%). 13) congenital or chromosomal abnormalities : VSD (38.7%), ASD (10.5%), TOF (8.4%), PDA (5.2%), CHPS (4.5%), Down syndrome (4.1%). 14) neonatal diseases : jaundice (32.0%), prematurity (12.7%), RDS (5.7%), sepsis (4.5%), birth asphyxia (3.0%), SGA (3.0%). 15) Others : febrile convulsion (45.6%), failure to thrive (9.2%), FUO (8.9%), drug intoxication (8.2%). 4. Common diseases for admission in pediatrics were in the following order : jaundice, pneumonia, unspecified acute gastroenteritis, VSD, RDS, URI, acute tonsillitis, acute lymphadenitis, ALL, sepsis, asthma, acute bronchiolitis, UTI.

함수언어 Miranda의 Serial-Combinator Graph로의 변역

윤영우,문성헌,최관덕 嶺南大學校 工業技術硏究所 1991 연구보고 Vol.19 No.1

One of the serious problems of functional languages is the slow speed in execution of functional programs. By choosing and desiging the appropriate reduction model and machine architecture and by using clever compiling techniques this problem can be overcome. The serial-combinator-based compiling technique is known as a very efficient one for the functional languages, especially in parallel processing. The writers developed a serial-combinator-based translator and an interpretative graph reducer for Sub Miranda, a test language, on the XENIX V system. The translator is composed of three phases. The first phase translates the SubMiranda source program into λ-calculus graph, the second phase transforms the λ-calculus graph into supercombinator graph, and the final phase transforms the supercombinator graph into serial combinator graph. The correctness of the translator was examined by the intermediate codes from translating a large number of the SubMiranda programs and by the results of reducing the translated serial combinator graphs.

여성 요실금에서 Kontinence™와 Compact Elite™의 치료효과 비교

양상국,정상원,오미희,노용수,김홍섭,우응제,박승훈,이수열 건국대학교 의과학연구소 1999 건국의과학학술지 Vol.9 No.-

Biofeedback and pelvic floor electrical stimulation are new modalities that have been advocated for the treatment of female urinary incontinence. The aim of this study was to compare prospectively the therapeutic effect of Kontinence™(HMT Co., Korea) with Compact Elite™(ECL Co., France) in female urinary incontinence. All patients were evaluated with a complete history, physical examination, urinalysis, urine culture and urodynamic study. Eighteen patients were treated with Compact Elite™, and thirteen patients with Kontinence™. Enrolled patients had completed 8 office sessions consisting of biofeedback and intravaginal electrical stimulation according to the program of each machines. Overall treatment response was graded by patients on a scale of complete cure to aggravation. At two weeks after last session, overall improvement rate was 78% in Compact Elite™group and 92% in Kontinence™group(p>0.05). Overall patients' acceptability during treatment course was greater than 80% in both machines(p>0.05). There were no unexpected clinical findings during Kontinence™ treatment. After Kontinence™treatment, there were no laboratory changes including electrocardiography, complete blood count, liver function profile, and chest PA. These results showed that the biofeedback and electrical stimulation with Kontinence™was safe and moderately effective for female urinary incontinence comparable to Compact Elite™. However, long-term results of a larger patients are needed to ensure the therapeutic effect of Kontinence™.

XPS와 AFM에 의한 올리고펩타이드의 자기 집합화 단분자막 분석

송성훈,박진영,김우식,Nakamura, Chikashi,Miyake, Jun,장상목 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.1

금속 기판 위에 유기 박막을 형성하는 방법 중에서 티올기와 Au와의 결합을 이용한 자기집합에 관한 연구가 활발히 진행되고 있다. 포르피린 유도체와 강하게 결합하는 타올기를 가지는 올리고펩타이드 PSP1과 PSP2를 합성하여 MOPS 완층용액에서 금표면에 자기집합화 단분자막(self-assembled monolayer, SAM)을 형성시켜 X-ray photoelection spectroscopy(XPS)와 atomic force microscopy(AFM)을 이용하여 분석하였다. 그 결과 PSP2의 경우와 PSP1의 경우보다 Au표면에서 SAM이 더욱 더 잘 형성되고 있음을 알 수 있었고 MOPS완충용액에 존재하는 황 이온의 영향도 알 수 있었다. 이 결과는 구성단위로부터 이론적으로 계산한 SAM막의 steric energy 값의 차이에서 유추한 결론과 일치하였다. Recently, the self-assembled monolayer(SAM) of thiols on nible metals has been studied intensively. Oligopeptides such as PSP1(Tyr-Ala-Gly-Tyr-Cys) and PSP2(His-Ala-Ser-Cys) with thiol group, which interact strongly with a typical cationic porphyrin derivative, are synthesized. Peptides adsorption from the liquid phase on Au has been studied using X-ray photoelection spectroscopy(XPS) and AFM image. XPS provides further evidence that the primary adsorbate species is bonded to Au through the sulfur atom. By the analysis of XPS spectra and AFM image, it is found that PSP2 is self-assembled better than PSP1 and the sulfur ion MOPS buffer solution has an effect on the self-assembling performance. This result is coincident with the calculation result of steric energies of Au-Peptide bonded SAM structure.

卷柏의 혈액암세포주에서 抗癌作用에 대한 연구

남항우,이성원,이병주,권칠용,문연자,안성훈,우원홍 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

The Sellaginella Tarmariscina is widely used in the traditional oriental herbal medicine for its anti-inflammatory, antioxidant effects. The effects of aqueous extracts of Sellaginella Tarmariscina on the viability and induction of apoptotic cell death were investigated in HL-60 cells. The cell viability after treating with extract of Sellaginella Tarmariscina was quantified by MTT assay. The results showed that could inhibit the proliferation of HL-60 cells and caused a 40% inhibition of HL-60 cells at concentrations of 400 ㎍/ml. The cancericidic effect of Sellaginella Tarmariscina was mediated by apoptosis. Thus, HL-60 cells exposed to Sellaginella Tarmariscina displayed the DNA fragmentation ladder and nucleus chromatin condensation characteristic for apoptosis. In conclusion, our results suggest that the extract of Sellaginella Tarmariscina may induce the apoptotic death in HL-60 cells.