Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells

Ruiping Zhang,Jing Li,Jianding Li,Jun Xie 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.9

Mesenchymal stem cells (MSCs) can differentiate into neural cells to treat nervous system diseases. Magnetic resonance is an ideal means for cell tracking through labeling cells with superparamagnetic iron oxide (SPIO). However, no studies have described the neural differentiation ability of SPIO-labeled MSCs, which is the foundation for cell therapy and cell tracking in vivo. Our results showed that bone marrow-derived mesenchymal stem cells (BM-MSCs) labeled in vitro with SPIO can be induced into neural-like cells without affecting the viability and labeling efficiency. The cellular uptake of SPIO was maintained after labeled BM-MSCs differentiated into neural-like cells, which were the basis for transplanted cells that can be dynamically and non-invasively tracked in vivo by MRI. Moreover, the SPIO-labeled induced neural-like cells showed neural cell morphology and expressed related markers such as NSE, MAP-2. Furthermore, whole-cell patch clamp recording demonstrated that these neural-like cells exhibited electrophysiological properties of neurons. More importantly, there was no significant difference in the cellular viability and [Ca2+]i between the induced labeled and unlabeled neural-like cells. In this study, we show for the first time that SPIO-labeled MSCs retained their differentiation capacity and could differentiate into neural-like cells with high cell viability and a good cellular state in vitro.

Laser-Induced Morphology Change Based on Small Molecular Model Compounds Photo-Detector

Ruiping Qin,Deen Guo,Jien Yang,Yurong Jiang 한국고분자학회 2018 Macromolecular Research Vol.26 No.11

Three small molecules furnished with a planar core, (5,6-bis(octyloxy) benzo[c][1,2,5] thiadiazole-4,7-diyl)bis(thiophene-5,2-diyl), and two wings, thieno [3,2-b]indole or carbazoles, were synthesized and used as sensing layer to make photo-detectors. The devices can detect photons at a wavelength band of 300-700 nm and exhibit a sensitive photo-response under on/off modulation. Near UV (405 nm) and visible (532 nm) laser beam were the light source in this work. The corrected responsivity reached up to 498 mA W-1 at 532 nm and 411 mA W-1 at 405 nm. Laser induced photo-current escalation was observed and investigated. For longer molecules, the photo-current was easy to be constant than the shorter reference counterpart. This special photo response mechanism was studied by alternating current impedance spectroscopy (IS) measurements and X-ray diffraction (XRD) exprements. Possible relations between molecular weight and various figures of merit for photo detectors were discussed.

Establishment of SSR Reaction System on Pear

Ruiping Ping Zhang,Shao Ling Zhang,Jun Wu 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Optimization of photocatalytic degradation of Cefradine using a “green” goethite/H2O2 system

Ruiping Li,Shaoming Hong,Xiaocong Li,Bin Zhang,Hailin Tian,Yingping Huang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.78 No.-

An environmental friendly photocatalyst, goethite in the presence of H2O2, was used to remove Cefradinefrom aqueous solution. Four factors were examined using Box–Behnken Design and results wereanalyzed by response surface method. H2O2 concentration had the largest effect on Cefradine removaland the optimal reaction conditions were: H2O2 concentration, 4 mmol/L; solution pH, 5; goethitedosage, 1.2 g/L and illumination time, 9 h. Experimental data on Cefradine removal under optimalconditions closely coincided with model predictions, validating the model. Hydroxyl radicals ( OH) andsuperoxide anion were involved in the Cefradine photodegradation process and that OH makes thelarger contribution.

Family-oriented Surrogate Decision Making and Confucian Bioethics

Fan, Ruiping Ewha Institute for Biomedical Law & Ethics 2011 BIOMEDICAL LAW & ETHICS Vol.5 No.2

This essay attempts to defend a family-oriented model of surrogate decision-making that prevails in some East Asian regions where Confucian virtue ethics is vitally influential. Although there are different standards suggested in the Western ethical and legal literatures regarding surrogate decision making for incapacitated patients, the dominant Western model of surrogate decision making is individual-oriented. In contrast, the Confucian familyoriented model takes into account the concerns of the family as a whole and allows the family to function from the bottom-up to make appropriate decisions for its incapacitated members. This essay shows that this model is rooted in the particular Confucian way of life shaped by the Confucian conceptions of authority, autonomy, decisional standard and mechanism. It also argues that the model can effectively deal with the challenges and problems raised against it in the contemporary medical world.

A rock physics simulator and its application for $CO_2$ sequestration process

Li, Ruiping,Dodds, Kevin,Siggins, A.F.,Urosevic, Milovan Korean Society of Earth and Exploration Geophysici 2006 지구물리와 물리탐사 Vol.9 No.1

Injection of $CO_2$ into underground saline formations, due to their large storage capacity, is probably the most promising approach for the reduction of $CO_2$ emissions into the atmosphere. $CO_2$ storage must be carefully planned and monitored to ensure that the $CO_2$ is safely retained in the formation for periods of at least thousands of years. Seismic methods, particularly for offshore reservoirs, are the primary tool for monitoring the injection process and distribution of $CO_2$ in the reservoir over time provided that reservoir properties are favourable. Seismic methods are equally essential for the characterisation of a potential trap, determining the reservoir properties, and estimating its capacity. Hence, an assessment of the change in seismic response to $CO_2$ storage needs to be carried out at a very early stage. This must be revisited at later stages, to assess potential changes in seismic response arising from changes in fluid properties or mineral composition that may arise from chemical interactions between the host rock and the $CO_2$. Thus, carefully structured modelling of the seismic response changes caused by injection of $CO_2$ into a reservoir over time helps in the design of a long-term monitoring program. For that purpose we have developed a Graphical User Interface (GUI) driven rock physics simulator, designed to model both short and long-term 4D seismic responses to injected $CO_2$. The application incorporates $CO_2$ phase changes, local pressure and temperature changes. chemical reactions and mineral precipitation. By incorporating anisotropic Gassmann equations into the simulator, the seismic response of faults and fractures reactivated by $CO_2$ can also be predicted. We show field examples (potential $CO_2$ sequestration sites offshore and onshore) where we have tested our rock physics simulator. 4D seismic responses are modelled to help design the monitoring program.

Efficient In Vitro Labeling Rabbit Bone Marrow-Derived Mesenchymal Stem Cells with SPIO and Differentiating into Neural-Like Cells

Zhang, Ruiping,Li, Jing,Li, Jianding,Xie, Jun Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.9

Mesenchymal stem cells (MSCs) can differentiate into neural cells to treat nervous system diseases. Magnetic resonance is an ideal means for cell tracking through labeling cells with superparamagnetic iron oxide (SPIO). However, no studies have described the neural differentiation ability of SPIO-labeled MSCs, which is the foundation for cell therapy and cell tracking in vivo. Our results showed that bone marrow-derived mesenchymal stem cells (BM-MSCs) labeled in vitro with SPIO can be induced into neural-like cells without affecting the viability and labeling efficiency. The cellular uptake of SPIO was maintained after labeled BM-MSCs differentiated into neural-like cells, which were the basis for transplanted cells that can be dynamically and non-invasively tracked in vivo by MRI. Moreover, the SPIO-labeled induced neural-like cells showed neural cell morphology and expressed related markers such as NSE, MAP-2. Furthermore, whole-cell patch clamp recording demonstrated that these neural-like cells exhibited electrophysiological properties of neurons. More importantly, there was no significant difference in the cellular viability and $[Ca^{2+}]_i$ between the induced labeled and unlabeled neural-like cells. In this study, we show for the first time that SPIO-labeled MSCs retained their differentiation capacity and could differentiate into neural-like cells with high cell viability and a good cellular state in vitro.

Mathematical modeling of torque for single screw expanders

Yuting Wu,Ruiping Zhi,Wei Wang,Lili Shen,Yeqiang Zhang,Biao Lei,Jingfu Wang,Chongfang Ma 대한기계학회 2017 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.31 No.1

This paper presents a mathematical model of torque for Single screw expanders (SSEs). Instantaneous torque and torque ratio were analyzed and discussed. The periodic variation of instantaneous torque is the same for different inlet pressure levels. The torque ratio, with its value close to 1, is independent of the inlet pressure of SSE. An experimental system was established to measure the torque, power and shaft efficiency of the self-developed SSE prototype, and results were used to validate the model. Comparison shows that the difference between calculated and experimental torque values is small (6.58 N.m to 7.55 N.m). The calculated and experimental output power is similar, with a difference of 2.07 kW to 2.37 kW. Therefore, the proposed model can be used to estimate the torque and output power of SSEs.

Down‑regulation expression of TGFB2‑AS1 inhibits the proliferation, migration, invasion and induces apoptosis in HepG2 cells

Wenrong Liu,Ruiping Huai,Yin Zhang,Shuquan Rao,Lili xiong,Ruofan Ding,Canquan Mao,Wenqing Zhao,Tao Hao,Qingqing Huang,Zhiyun Guo 한국유전학회 2019 Genes & Genomics Vol.41 No.8

Background Hepatocellular carcinoma (HCC) is the leading cause of cancer mortality and without effective prognosis. Previous study has been confirmed that the abnormal expression of long non-coding RNAs (lncRNAs) TGFB2-AS1 was involved in tumorigenesis. However, the biological functions of TGFB2-AS1 in hepatocellular carcinoma (HCC) remain largely unclear. Objective We comprehensively assess the clinical significance of TGFB2-AS1 and investigate the biological functions of TGFB2-AS1 on HCC HepG2 cells. Methods We firstly confirmed the expression of TGFB2-AS1 between tumor and normal tissues using public available transcriptome data. We analyzed the clinical significance of TGFB2-AS1 using the TCGA HCC datasets. The biological functions of TGFB2-AS1 on HCC HepG2 cells were explored by multiple in vitro assays. Results We found that TGFB2-AS1 was remarkably increased in HCC tissues (P = 0.00148) and exhibited a potential predictive marker for HCC, with an area under curve (AUC) of 0.708 (P = 0.0034) using the fifty pairs of matched HCC tissues of TCGA. Besides, higher expression of TGFB2-AS1 in HCC tissues was identified as being positively associated with advanced tumor (P = 0.012) and disease stage (P = 0.009) in 355 HCC cases using independent sample nonparametric test. Downregulation of TGFB2-AS1 expression significantly restrained proliferation (P < 0.01) and impaired colony formation (P < 0.05). Furthermore, TGFB2-AS1 depletion remarkably promoted the apoptosis of HepG2 cells (P < 0.05) and inhibited migration and invasion (P < 0.01). Conclusion Taken together, these findings suggested that TGFB2-AS1 might serve as a potential therapeutic target for HCC.

Polyurethane Membrane with Temperature- and pH-Controllable Permeability for Amino-Acids

Hu Zhou,Ruiping Xun,Kejian Wu,Zhihua Zhou,Bin Yu,Youxin Tang,Ning Li 한국고분자학회 2015 Macromolecular Research Vol.23 No.1

This work was focused on the investigation of the temperature- and pH-responsive polyurethane (PU)membranes and their permeability to amino-acids in response to environmental stimuli. The PU membrane wasprepared from a wet phase inversion method and a two-step solution polymerization from polycaprolactone diols(PCL), 4,4′-diphenylmethane diisocyanate (MDI), dimethylol propionic acid (DMPA), etc. The chemical structure,phase state, morphology and surface wettability of the membrane were characterized with Fourier transform infrared(FTIR) spectrometer, differential scanning calorimetry (DSC), scanning electron microscopy (SEM) and contactangle tester, respectively. The temperature and pH responses of the membrane were investigated by means of anamino-acid permeate experiment. The L-phenylalanine (L-Phe) was chosen as model amino-acids. The permeation of theL-Phe was measured using a dead-end flow filtration at varied temperatures and pH, and characterized by the permeateflux (J) and rejection coefficient (R). J of the L-Phe across the PU membrane increased with increasingtemperature and showed a sharp increase when temperature was raised to the crystalline melting temperature (Tm)of the soft segment of PU, while decreased with increasing pH and having a sharp decrease when pH reached thedissociation constant (pKa) of DMPA contained in PU macromolecules. While, the R behavior of L-Phe was justopposite from the results of J, which decreased with increasing temperature and increased with increasing pH,also showing the temperature and pH responses. Hopefully, the PU membrane with temperature- and pH-controllablepermeability has promising prospects in water treatment, membrane separation, drug delivery system, etc.