Study on Enhancing Lightning Protection Scheme of Catenary in Subway Viaduct Section

Li, Rui-Fang,Chen, Kui,Chen, Li-Sheng,Cao, Xiao-Bin,Wu, Guang-Ning,Zhang, Xue-Qin The Korean Institute of Electrical Engineers 2017 Journal of Electrical Engineering & Technology Vol.12 No.2

Viaduct increases the height of subway catenary, namely magnifies lightning attraction scope that lead to higher possibility of suffering lightning stroke. Therefore, it is necessary to analyze performance of lightning striking to catenary of subway in viaduct section and propose an improving lightning protection scheme. In this paper, using ATP-EMTP simulation software to establish an associated model to evaluate lightning withstand level of catenary with existing lightning protection schemes including arrester and grounding point, an improving lightning protection scheme is proposed - every pillar ground earth and arresters are installed with some installing spacing between 200m to 400m based on lightning damage degree and reliability requirements - according to analyzing results: while lightning withstand level is lowest for lightning striking to the neutral pillar, lightning withstand level is greatest for lightning striking to the both-ends pillar that arrester and grounding point are both installed; grounding point could obviously improve lightning withstand level for lightning striking to ground wire while arrester could obviously improve the lightning withstand level for lightning striking to catenary; every pillar ground earth could enhance the lowest lightning withstanding level up to 2.5 times than of that pillar ground earth across every 200m.

Synthesis and Antibacterial Activity of 1,3-Diallyltrisulfane Derivatives

Fang-Kui Ren,Xiao-Yan He,Li Deng,Bo-Heng Li,Dong-Soo Shin,Zhu-Bo Li 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.3

A series of novel 1,3-diallyltrisulfane analogues were synthesized and assayed in vitro for antimicrobial activity against Gram positive, Gram negative bacteria and fungi. The antimicrobial activity of the 1,3-diallyltrisulfane derivatives showed, on the whole, very potent towards all the tested Gram positive, Gram negative and fungi (MIC ranging from 4 to 256 μg/mL). 1,3-Di(pent-4-enyl)trisulfane 3b and 1,3-bis(3-methylbut-2-enyl)trisulfane 3e exhibited the strongest antibacterial activity among all the compounds, and both of them were more active than 1,3-diallyltrisulfane (DATS). Results indicated the relationship of either carbon number or lipophilicity with antimicrobial activity presented “V” shape. These observations provided some predictions in order to further design 1,3-diallyltrisulfane derivatives with antimicrobial activity.

Study on Enhancing Lightning Protection Scheme of Catenary in Subway Viaduct Section

Rui-Fang Li,Kui Chen,Li-Sheng Chen,Xiao-Bin Cao,Guang-Ning Wu,Xue-Qin Zhang 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.2

Viaduct increases the height of subway catenary, namely magnifies lightning attraction scope that lead to higher possibility of suffering lightning stroke. Therefore, it is necessary to analyze performance of lightning striking to catenary of subway in viaduct section and propose an improving lightning protection scheme. In this paper, using ATP-EMTP simulation software to establish an associated model to evaluate lightning withstand level of catenary with existing lightning protection schemes including arrester and grounding point, an improving lightning protection scheme is proposed — every pillar ground earth and arresters are installed with some installing spacing between 200m to 400m based on lightning damage degree and reliability requirements — according to analyzing results: while lightning withstand level is lowest for lightning striking to the neutral pillar, lightning withstand level is greatest for lightning striking to the both-ends pillar that arrester and grounding point are both installed; grounding point could obviously improve lightning withstand level for lightning striking to ground wire while arrester could obviously improve the lightning withstand level for lightning striking to catenary; every pillar ground earth could enhance the lowest lightning withstanding level up to 2.5 times than of that pillar ground earth across every 200m.

Synthesis and Antibacterial Activity of 1,3-Diallyltrisulfane Derivatives

Ren, Fang-Kui,He, Xiao-Yan,Deng, Li,Li, Bo-Heng,Shin, Dong-Soo,Li, Zhu-Bo Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.3

A series of novel 1,3-diallyltrisulfane analogues were synthesized and assayed in vitro for antimicrobial activity against Gram positive, Gram negative bacteria and fungi. The antimicrobial activity of the 1,3-diallyltrisulfane derivatives showed, on the whole, very potent towards all the tested Gram positive, Gram negative and fungi (MIC ranging from 4 to 256 μg/mL). 1,3-Di(pent-4-enyl)trisulfane 3b and 1,3-bis(3-methylbut-2-enyl)trisulfane 3e exhibited the strongest antibacterial activity among all the compounds, and both of them were more active than 1,3-diallyltrisulfane (DATS). Results indicated the relationship of either carbon number or lipophilicity with antimicrobial activity presented “V” shape. These observations provided some predictions in order to further design 1,3-diallyltrisulfane derivatives with antimicrobial activity.

The Effects of Physicochemical Factors and Cell Density on Nitrite Transformation in a Lipid-Rich Chlorella

( Fang Liang ),( Kui Du ),( Xiaobin Wen ),( Liming Luo ),( Yahong Geng ),( Yeguang Li ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.12

To understand the effects of physicochemical factors on nitrite transformation by microalgae, a lipid-rich Chlorella with high nitrite tolerance was cultured with 8 mmol/l sodium nitrite as sole nitrogen source under different conditions. The results showed that nitrite transformation was mainly dependent on the metabolic activities of algal cells rather than oxidation of nitrite by dissolved oxygen. Light intensity, temperature, pH, NaHCO3 concentrations, and initial cell densities had significant effects on the rate of nitrite transformation. Single-factor experiments revealed that the optimum conditions for nitrite transformation were light intensity: 300 μmol/㎡/s; temperature: 30℃; pH: 7-8; NaHCO3 concentration: 2.0 g/l; and initial cell density: 0.15 g/l; and the highest nitrite transformation rate of 1.36 mmol/l/d was achieved. There was a positive correlation between nitrite transformation rate and the growth of Chlorella. The relationship between nitrite transformation rate (mg/l/d) and biomass productivity (g/l/d) could be described by the regression equation y = 61.3x (R(2) = 0.9665), meaning that 61.3 mg N element was assimilated by 1.0 g dry biomass on average, which indicated that the nitrite transformation is a process of consuming nitrite as nitrogen source by Chlorella. The results demonstrated that the Chlorella suspension was able to assimilate nitrite efficiently, which implied the feasibility of using flue gas for mass production of Chlorella without preliminary removal of NOX.

Utilization of Single Nodal Cuttings and Therapies for Eradicating Double-Infected Potato Virus (PLRV, PVY) from In Vitro Plantlets of Potato (Solanum tuberosum)

Yi Lan Fang,Shambbu Prasad Dhital,Kui Hua Li,Dong Man Khu,Hae Young Kim,Ye Su Song,Hak Tae Lim 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.2

In vitro plantlets of potato genotype ‘F9-99’ infected with PLRV and PVY were used for the virus eradication using heat and chemical treatments. Subculture medium was added with acetylsalicylic acid (ASA) (10??, 10??, and 10?⁴ M) and ribavirin (10, 15, and 20 ㎎ㆍL?¹) alone or in combinations. The plantlets were incubated at 25℃ (control), and alternating heat treatments 35/30℃ for 1, 4, and 8 h or 42/25℃ for 4 h. Virus status of the plantlets were examined by means of double antibody sandwich-enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction. After 4?8 weeks of treatment, 40, 51, 41, 36, and 0% survival were observed in the control (MS and 25℃), ASA plus ribavirin (25℃), MS plus heat treatment (35/30℃), ASA plus ribavirin (35/30℃), and MS medium with heat treatment (42/25℃), respectively. As for virus eradication, heat treatment (35/30℃) with 1 h alternating thermo-cycle resulted in 100% PVY elimination, but it was much more difficult to remove the PLRV from the double-infected potato plantlets with PVY and PLRV. The highest rate of PLRV eradication was 60% from the combined treatment of 10?? M ASA, 20 ㎎ㆍL?¹ ribavirin, and heat treatment at 35/30℃ for 8 h alternating temperature.

Physiological character and molecular mapping of leaf-color mutant wyv1 in rice (Oryza sativa L.)

Xian-chun Sang,Li-kui Fang,Yuenyong Vanichpakorn,Ying-hua Ling,Peng Du,Fang-ming Zhao,Zheng-lin Yang,Guang-hua He 한국유전학회 2010 Genes & Genomics Vol.32 No.2

The seed of an excellent indica restorer line Jinhui10 (Oryza sativa L. ssp. indica) was treated by ethyl methanesulfonate (EMS); a leaf-color mutant displaying distinct phenotype throughout development grown in paddy field was identified from the progeny. The mutant leaf showed white-yellow at seedling stage and then turned to yellow-green at tillering stage, after that, virescent color appeared until to maturity. The mutant was thus temporarily designed as wyv1. The chlorophyll contents decreased significantly and the changing was consistent with the chlorotic level of wyv1 leaves. Chlorophyll fluorescence kinetic parameters measured at the seedling stage showed that co-efficiency of photochemical quenching (qP),actual photosystem II efficiency (ΦPS II), electron transport rate (ETR) and initial chlorophyll fluorescence level (Fo), net photosynthetic rate (Pn) and maximum photochemical efficiency (Fv / Fm) significantly decreased in severe chlorotic leaf of the mutant compared with that of wild type. However,no significant differences were observed for Pn and Fv/Fm between virescent leaf and normal green leaf. Genetic analysis suggested that the mutant phenotype was controlled by a single recessive nuclear gene which was finally mapped between SSR marker Y7 and Y6 on rice chromosome 3 based on F2 population of Xinong1A / wyv1. Genetic distances were 0.06 cM and 0.03 cM respectively, and the physical distance was 84kb according to the sequence of indica rice 9311. The results must facilitate map-based cloning and functional analysis of WYV1 gene.

Gene cloning and functional analysis of yellow green leaf 3 (ygl3) gene during the whole-plant growth stage in rice

Xiaoqing Tian,Ying-Hua Ling,Li-kui Fang,Peng Du,Xian-Chun Sang,Fang-Ming Zhao,Yun-Feng Li,Rong Xie,Guang-Hua He 한국유전학회 2013 Genes & Genomics Vol.35 No.1

Chlorophyll is an important photosynthetic pigment in the process of photosynthesis in plants and photosynthetic bacteria. Genes involved in chlorophyll biosynthesis in Arabidopsis and photosynthetic bacteria have been well documented. In rice, however, these genes have not been fully annotated. In this paper, a yellow-green leaf gene, yellow green leaf3 (ygl3) was cloned and analyzed. ygl3 encodes magnesium chelation ChlD (D) subunit,a key enzyme for chlorophyll synthesis, resulting in a yellow-green leaf phenotype in all growth stages in rice. Expression content of ygl3 is highest in the leaf blades,followed by the leaf sheaths, while there is virtually no expression of the gene in the stems and seeds. The subcellular structure and protein content of the photosynthetic system of the ygl3 mutant were revealed by transmission electron microscopy, BN-PAGE, and western blotting. The results show that the mutation of the ygl3 gene indirectly leads to a decrease in the protein content of the photosynthetic system and severely obstructs the formation of granum thylakoids.

두 가지 제초제에 대하여 저항성을 가지는 항생제 마커-프리 형질전환 감자 육성

방일란,김진석,공수,모황성,민석기,권석윤,이규화,임학태,Fang, Yi-Lan,Kim, Jin-Seog,Gong, Su,Mo, Hwang-Suk,Min, Seok-Ki,Kwon, Suk-Yoon,Li, Kui-Hua,Lim, Hak-Tae 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.3

본 연구에서는 제초제 저항성 bar 유전자 및 CP4-EPSPS 유전자를 포함하는 발현벡터로 형질전환되고 항생제 마커 유전자를 포함하지 않는 제초제 복합 저항성 감자 식물체를 육성하고자 실험하였다. Bar 유전자를 포함하는 pCAMBIA3300에 CaMV35S 프로모터에 의해 조절되는 CP4-EPSPS 유전자를 도입하여 식물체용 발현 운반체를 제작하고, 이를 Agrobacterium tumafaciens EHA105에 도입하였다. 태동밸리 잎 절편체를 Agrobacterium과 공동배양한 다음, phosphinothricin 0.5 mg/L이 첨가된 배지에서 선발하고 호르몬 무처리 MS발근시켜 형질전환체 (E3-6)를 얻었다. PCR, Southern 분석, 효소면역반응 분석 등을 통해 두 가지 유전자가 도입되었으며 이들이 정상적으로 발현됨이 확인되었다. E3-6 식물체는 glufosinate-ammonium의 어린 식물체 잎 도포처리, glyphosate 용액에 치상한 식물체 조직에서의 shikimate 축적 여부 조사를 통하여 조사한 결과, 두 제초제에 대해 저항성을 나타내었다. 또한 형질전환감자의 전식물체에 대해 glyphosate와 glufosinate-ammonium 각각의 용액 또는 이들의 혼합물을 처리한 후 제초활성 반응을 조사한 결과, E3-6 형질전환 감자는 두 제초제를 각각 단독으로 처리할 때나 혼합하여 동시 처리할 때에도 동일한 저항성이 나타남을 확인하였다. This study was conducted to develop an antibiotics marker-free potato (Solanum tuberosum L., cv. Taedong valley) plant having resistance against two herbicides. Agrobacterium tumefaciens strain EHA105, harboring a binary vector plasmid pCAMBIA3300 containing bar gene under the control of a promoter CaMV35S and linked CP4-EPSPS genes driven by CaMV35S promoter, was used in the current study. The leaf segments of newly bred potato variety (cv. Taedong Valley) was co-cultured with Agrobacterium. Then, the regenerated individual shoots were excised and transferred to potato multiplication medium supplemented with 0.5 mg/L phosphinothricin. The shoots were rooted in MS medium without hormone and obtained putative transgenic plant E3-6. Integration of target genes into the E3-6 plant and their expression was confirmed by PCR, Southern analysis, and ELISA test. The tissue necrosis test on young leaf blade and shikimic acid accumulation test using the tissue of E3-6 plant were conducted to investigate the resistance to glufosinate-ammonium and glyphosate, respectively. The transgenic plants (E3-6) simultaneously showed a high resistance to both herbicides. The same results were surely obtained also in the whole plants foliar-treated with alone or mixture of two herbicides, glufosinate-ammonium and glyphosate.