A Novel Molecular Grading Model: Combination of Ki67 and VEGF in Predicting Tumor Recurrence and Progression in Non-invasive Urothelial Bladder Cancer

Chen, Jun-Xing,Deng, Nan,Chen, Xu,Chen, Ling-Wu,Qiu, Shao-Peng,Li, Xiao-Fei,Li, Jia-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Purpose: To assess efficacy of Ki67 combined with VEGF as a molecular grading model to predict outcomes with non-muscle invasive bladder cancer (NMIBC). Materials: 72 NMIBC patients who underwent transurethral resection (TUR) followed by routine intravesical instillations were retrospectively analyzed in this study. Univariate and multivariate analyses were performed to confirm the prognostic values of the Ki67 labeling index (LI) and VEGF scoring for tumor recurrence and progression. Results: The novel molecular grading model for NMIBC contained three molecular grades including mG1 (Ki67 $LI{\leq}25%$, VEGF $scoring{\leq}8$), mG2 (Ki67 LI>25%, VEGF $scoring{\leq}8$; or Ki67 $LI{\leq}25%$, VEGF scoring > 8), and mG3 (Ki67 LI > 25%, VEGF scoring > 8), which can indicate favorable, intermediate and poor prognosis, respectively. Conclusions: The described novel molecular grading model utilizing Ki67 LI and VEGF scoring is helpful to effectively and accurately predict outcomes and optimize personal therapy.

Network topology control strategy based on spatial evolutionary public goods game

Li, Zhi,Deng, Chuang,Suh, Il Hong Elsevier 2015 PHYSICA A-STATISTICAL MECHANICS AND ITS APPLICATIO Vol.432 No.-

<P><B>Abstract</B></P> <P>It is often the case that rational individuals will adjust their connectivity in commercial or social activities for maximizing their payoffs. In this process, we can observe that individuals always gather around a leader or a competitive individual who can bring them more benefits. Inspired by this, we propose a strategy that impels nodes of network to connect with a specific node that we have specified with the perspective of spatial evolutionary public goods game. Thus a node is specified and given a larger enhancement factor which reflects his advantage over others. Then we employ a payoff-oriented preferential rewire strategy that individual will sever a neighbor who provides him with the lowest benefit and then link others randomly. The results illustrate that this strategy not only ensures the promotion of cooperation but also increases the degree of the specified node. Furthermore, we analyze the effect of two relevant parameters: enhancement factor and rewire frequency. We find that if the control strategy expects to work effectively, these two parameters have to ensure an evolution environment where cooperators can prevail defectors. We also conclude that a relatively low rewire frequency contributes to increasing the degree of the specified node. Meanwhile we attempt to present our interpretations for these phenomena.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The phenomenon that competitive individual will attract more partners is interpreted with the framework of spatial evolutionary game theory. </LI> <LI> A strategy that impels nodes of network to connect with a specified node is proposed. </LI> <LI> Cooperative environment is essential for attractive operation. </LI> <LI> Low rewire frequency leads to high level of heterogeneity. </LI> </UL> </P>

Photoluminescence properties of a new orange–red emitting Sm³⁺-doped Y₂Mo₄O₁₅ phosphor

Deng, Huajuan,Zhao, Ze,Wang, Jing,Hei, Zhoufei,Li, Mengxue,Noh, Hyeon Mi,Jeong, Jung Hyun,Yu, Ruijin Elsevier 2015 Journal of solid state chemistry Vol.228 No.-

<P><B>Abstract</B></P> <P>A series of novel Y<SUB>2</SUB>Mo<SUB>4</SUB>O<SUB>15</SUB>:<I>x</I>Sm<SUP>3+</SUP> ( (0.01 ≤ <I>x</I> ≤ 0.20) phosphors for white light-emitting (W-LEDs) were successfully prepared by the solid state reaction technology at 973K for 12h. X-ray diffraction and photoluminescence spectra were utilized to characterize the structure and luminescence properties of the as-synthesized phosphors. The emission spectra of the Y<SUB>2</SUB>Mo<SUB>4</SUB>O<SUB>15</SUB>:Sm<SUP>3+</SUP> phosphors consisted of some sharp emission peaks of Sm<SUP>3+</SUP> ions centered at 565nm, 605nm, 650nm, and 712nm. The strongest one is located at 605nm due to <SUP>4</SUP> <I>G</I> <SUB>5/2</SUB>–<SUP>6</SUP> <I>H</I> <SUB>7/2</SUB> transition of Sm<SUP>3+</SUP>, generating bright orange–red light. The optimum dopant concentration of Sm<SUP>3+</SUP> ions in Y<SUB>2</SUB>Mo<SUB>4</SUB>O<SUB>15</SUB>:<I>x</I>Sm<SUP>3+</SUP> is around 5mol% and the critical transfer distance of Sm<SUP>3+</SUP> is calculated as 23.32Å. The CIE chromaticity coordinates of the Y<SUB>2</SUB>Mo<SUB>4</SUB>O<SUB>15</SUB>:0.05Sm<SUP>3+</SUP> phosphors were located in the orange reddish region. The Y<SUB>2</SUB>Mo<SUB>4</SUB>O<SUB>15</SUB>:Sm<SUP>3+</SUP> phosphors may be potentially used as red phosphors for white light-emitting diodes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An orange–red emitting Y<SUB>2</SUB>Mo<SUB>4</SUB>O<SUB>15</SUB>:Sm<SUP>3+</SUP> phosphor has been firstly synthesized. </LI> <LI> Their structures, luminescent properties have also been investigated. </LI> <LI> The optical absorption edge for the molybdate lies around 325nm. </LI> <LI> The CIE chromaticity coordinates were located in the orange reddish region. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>The excitation spectrum of Y<SUB>2</SUB>Mo<SUB>4</SUB>O<SUB>15</SUB>:Sm<SUP>3+</SUP> is composed of a broad band and some sharp <I>f</I>–<I>f</I> transitions. Under 407nm excitation, the phosphor presents some sharp emission peaks of Sm<SUP>3+</SUP> ions.</P> <P>[DISPLAY OMISSION]</P>

Advances in CRISPR-Cas systems for RNA targeting, tracking and editing

Wang, Fei,Wang, Lianrong,Zou, Xuan,Duan, Suling,Li, Zhiqiang,Deng, Zixin,Luo, Jie,Lee, Sang Yup,Chen, Shi Elsevier 2019 BIOTECHNOLOGY ADVANCES Vol.37 No.5

<P><B>Abstract</B></P> <P>Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, especially type II (Cas9) systems, have been widely used in gene/genome targeting. Modifications of Cas9 enable these systems to become platforms for precise DNA manipulations. However, the utilization of CRISPR-Cas systems in RNA targeting remains preliminary. The discovery of type VI CRISPR-Cas systems (Cas13) shed light on RNA-guided RNA targeting. Cas13d, the smallest Cas13 protein, with a length of only ~930 amino acids, is a promising platform for RNA targeting compatible with viral delivery systems. Much effort has also been made to develop Cas9, Cas13a and Cas13b applications for RNA-guided RNA targeting. The discovery of new RNA-targeting CRISPR-Cas systems as well as the development of RNA-targeting platforms with Cas9 and Cas13 will promote RNA-targeting technology substantially. Here, we review new advances in RNA-targeting CRISPR-Cas systems as well as advances in applications of these systems in RNA targeting, tracking and editing. We also compare these Cas protein-based technologies with traditional technologies for RNA targeting, tracking and editing. Finally, we discuss remaining questions and prospects for the future.</P> <P><B>Highlights</B></P> <P> <UL> <LI> RNA targeting and editing are becoming increasingly important </LI> <LI> CRISPR-Cas systems are advancing for RNA targeting, tracking and editing </LI> <LI> The type VI CRISPR-Cas systems are useful for RNA-guided RNA targeting </LI> <LI> Use of Cas9 and Cas13 will advance RNA-targeting technologies </LI> </UL> </P>

SERS study of surface plasmon resonance induced carrier movement in Au@Cu₂O core-shell nanoparticles

Chen, Lei,Zhang, Fan,Deng, Xin-Yu,Xue, Xiangxin,Wang, Li,Sun, Yantao,Feng, Jing-Dong,Zhang, Yongjun,Wang, Yaxin,Jung, Young Mee Elsevier 2018 Spectrochimica acta. Part A, Molecular and biomole Vol.189 No.-

<P><B>Abstract</B></P> <P>A plasmon induced carrier movement enhanced mechanism of surface-enhanced Raman scattering (SERS) was investigated using a charge-transfer (CT) enhancement mechanism. Here, we designed a strategy to study SERS in Au@Cu<SUB>2</SUB>O nanoshell nanoparticles with different shell thicknesses. Among the plasmonically coupled nanostructures, Au spheres with Cu<SUB>2</SUB>O shells have been of special interest due to their ultrastrong electromagnetic fields and controllable carrier transfer properties, which are useful for SERS. Au@Cu<SUB>2</SUB>O nanoshell nanoparticles (NPs) with shell thicknesses of 48–56nm are synthesized that exhibit high SERS activity. This high activity originates from plasmonic-induced carrier transfer from Au@Cu<SUB>2</SUB>O to 4-mercaptobenzoic acid (MBA). The CT transition from the valence band (VB) of Cu<SUB>2</SUB>O to the second excited π-π* transition of MBA, and is of b<SUB>2</SUB> electronic symmetry, which was enhanced significantly. The Herzberg-Teller selection rules were employed to predict the observed enhanced b<SUB>2</SUB> symmetry modes. The system constructed in this study combines the long-range electromagnetic effect of Au NPs, localized surface plasmon resonance (LSPR) of the Au@Cu<SUB>2</SUB>O nanoshell, and the CT contribution to assist in understanding the SERS mechanism based on LSPR-induced carrier movement in metal/semiconductor nanocomposites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We designed a shell-dependent Au@Cu<SUB>2</SUB>O nanoshell for SERS study. </LI> <LI> SERS contribution enables us to understand the possible enhancement of hybrid nanostructures. </LI> <LI> LSPR-induced carrier movement in Au@Cu<SUB>2</SUB>O nanocomposites. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>We designed the shell-dependent Au@Cu<SUB>2</SUB>O core-shell nanoparticles (NPs) for SERS study. For the electron-hole pairs in the Cu<SUB>2</SUB>O, the plasmon induced resonant energy transfer from Au to the Cu<SUB>2</SUB>O and the direct electron transfer simultaneously which can be observed from the SERS intensity.</P> <P>[DISPLAY OMISSION]</P>

Stress-induced expression of the sweetpotato gene <i>IbLEA14</i> in poplar confers enhanced tolerance to multiple abiotic stresses

Ke, Qingbo,Park, Sung-Chul,Ji, Chang Yoon,Kim, Ho Soo,Wang, Zhi,Wang, Shiwen,Li, Hongbing,Xu, Bingcheng,Deng, Xiping,Kwak, Sang-Soo Elsevier 2018 Environmental and experimental botany Vol.156 No.-

<P><B>Abstract</B></P> <P>Late embryogenesis abundant (LEA) proteins are small, highly hydrophilic proteins that act as protectors of macromolecules and increase abiotic stress tolerance in plants. We previously reported that overexpressing sweetpotato <I>IbLEA14</I> under the control of the <I>CaMV 35S</I> promoter increased osmotic and salt stress tolerance in transgenic sweetpotato calli. In this study, we generated transgenic poplar plants (<I>Populus alba × P. glandulosa</I>) expressing <I>IbLEA14</I> under the control of the oxidative stress-inducible <I>SWPA2</I> promoter (referred to as SL plants). Among the 15 SL plants obtained, three lines (SL2, SL7, and SL12) were established based on <I>IbLEA14</I> transcript levels, tolerance to salt stress and Southern blot analysis. The SL plants exhibited less damage in response to methyl viologen-mediated oxidative stress than non-transgenic (NT) plants. SL plants also showed enhanced tolerance to drought, salt, and heat stress, which was associated with higher photosystem II efficiency and lower malondialdehyde levels compared with NT plants. Furthermore, SL plants had higher levels of monolignol biosynthesis-related gene transcripts under drought stress compared with NT plants. Finally, SL plants exhibited increased tolerance to heat stress, which is associated with the high thermostability of IbLEA14 protein. SL plants might be useful for reforestation on global marginal lands, including desertification and reclaimed areas.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>IbLEA14</I> gene was transformed into poplar plants. </LI> <LI> Transgenic poplars exhibit increased tolerance to MV-mediated oxidative, drought, salt and heat stress. </LI> <LI> Overproduction of IbLEA14 affects lignification and thermostability of transgenic poplars. </LI> </UL> </P>

Overexpression of Cyclin L2 Inhibits Growth and Enhances Chemosensitivity in Human Gastric Cancer Cells

Li, Hong-Li,Huang, Ding-Zhi,Deng, Ting,Zhou, Li-Kun,Wang, Xia,Bai, Ming,Ba, Yi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Cyclin L2 is a novel member of the cyclin family, recently implicated in the regulation of cell cycle progression and/or transcriptional regulation. The present study was undertaken to investigate the effects of overexpression on tumor cell growth and chemosensitivity in human gastric cells in vitro. Cyclin L2 was transfected into human gastric cancer cell line BCG823 and expressed with a mammalian expression vector pcDNA3.1. The effects and mechanisms of cyclin L2 on cell growth, cell cycling and apoptosis were studied. Compared to control vectors, overexpression of cyclin L2 inhibited the growth of BCG823 cells and enhance their chemosensitivity to fluorouracil, docetaxel and cisplatin. The anti-proliferative effects of cyclin L2 could be due to G0/G1 arrest and apoptosis. Cyclin L2 induced G0/G1 arrest and apoptosis involved upregulation of caspase-3 and down regulation Bcl-2 and survivin. The results indicated that overexpression of cyclin L2 protein may promote efficient growth inhibition and enhance chemosensitivity to chemotherapeutic agents in human gastric cancer cells by inducing G0/G1 cell cycle arrest and apoptosis.

전통문화를 반영한 중국 현대 주얼리 스타일의 이미지 평가 연구

이등등(Li, Deng Deng),성광숙(Sung Kwangsook) 한국일러스아트학회 2023 조형미디어학 Vol.26 No.1

Silencing downstream of receptor kinase gene (drk) impairs larval-pupal ecdysis in Leptinotarsa decemlineata (Say)

Pan Deng,Jun-Li Du,Li-Li Mu,Kai-Yun Fu,Wen-Chao Guo,Guo-Qing Li 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.1

In insects, an insulin-like peptide (ILP) triggers the formation of the insulin receptor (InR)/the insulin receptor substrate Chico complex. The complex then recruits downstream of receptor kinase (Drk) and phosphatidylinositol-3-kinase (PI3K) to initiate two signaling branches, i.e., Drk-mitogen-activated protein kinase (MAPK) and Pi3K-protein kinase B subdivisions. Previous findings reveal that RNA interference (RNAi) of LdILP2 or Ldchico, rather than Ldpi3k92E, impairs larval-pupal and pupal-adult molting in the Colorado potato beetle Leptinotarsa decemlineata. It is accordingly hypothesized that the Drk-MAPK branch regulates larval metamorphosis. In the present paper, we first found that silencing LdILP2, Ldchico or Ldpi3k92E did not decrease the expression level of Lddrk, indicating other receptor tyrosine kinases’ signaling except insulin pathway is not affected in the RNAi larvae. Moreover, two InRs and Torso were highly expressed in the final larval instars. Furthermore, RNAi of either Lddrk or Ldchico, or both of them equally affected larval-pupal and pupal-adult molts, and similarly repressed the expression of representative MAPK (Ldras and Ldraf), ecdysteroidogenesis (Ldphm and Ldsad), and 20E signaling (LdEcR, LdUSP, LdHR3 and LdE75) genes. 20E feeding by Lddrk RNAi larvae completely restored the reduced mRNA levels of LdEcR, LdHR3 and LdE75, but did not rescued the decreased Lddrk and LdUSP levels and the lowered pupation and emergence rates. Therefore, our findings suggest that the Drk-MAPK branch is involved in metamorphosis regulation in L. decemlineata.

Protocatechuic Aldehyde Represses Proliferation and Migration of Breast Cancer Cells through Targeting C-terminal Binding Protein 1

Yu Deng,Wanjun Guo,Guancheng Li,Shuang Li,Hong Li,Xinyan Li,Bei Niu,Mingzhu Song,Yamei Zhang,Zhijian Xu,Fulun Li 한국유방암학회 2020 Journal of breast cancer Vol.23 No.1

Purpose: C-terminal binding protein 1 (CtBP1) is a transcriptional co-repressor that is overexpressed in many cancers. CtBP1 transcriptionally represses a broad array of tumor suppressors, which promotes cancer cell proliferation, migration, invasion, and resistance to apoptosis. Recent studies have demonstrated that CtBP1 is a potential target for cancer therapy. This study was designed to screen for compounds that potentially target CtBP1. Methods: Using a structure-based virtual screening for CtBP1 inhibitors, we found protocatechuic aldehyde (PA), a natural compound found in the root of a traditional Chinese herb, Salvia miltiorrhiza, that directly binds to CtBP1. Microscale thermophoresis assay was performed to determine whether PA and CtBP1 directly bind to each other. Further, clustered regularly interspaced short palindromic repeats associated Cas9 nuclease-mediated CtBP1 knockout in breast cancer cells was used to validate the CtBP1 targeting specificity of PA. Results: Functional studies showed that PA repressed the proliferation and migration of breast cancer cells. Furthermore, PA elevated the expression of the downstream targets of CtBP1, p21 and E-cadherin, and decreased CtBP1 binding affinity for the promoter regions of p21 and E-cadherin in breast cancer cells. However, PA did not affect the expression of p21 and E-cadherin in the CtBP1 knockout breast cancer cells. In addition, the CtBP1 knockout breast cancer cells showed resistance to PA-induced repression of proliferation and migration. Conclusion: Our findings demonstrated that PA directly bound to CtBP1 and inhibited the growth and migration of breast cancer cells through CtBP1 inhibition. Structural modifications of PA are further required to enhance its binding affinity and selectivity for CtBP1.