Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

제주도 지하수 질산염 농도의 시·공간적 변화 특성: 장기(1993-2015) 모니터링 자료의 평가

김호림(Ho-Rim Kim),오준섭(Junseop Oh),도현권(Hyun-Kwon Do),이경진(Kyung-Jin Lee),현익현(Ik-Hyun Hyun),오상실(Sang-Sil Oh),감상규(Sang-Kyu Kam),윤성택(Seong-Taek Yun) 대한자원환경지질학회 2018 자원환경지질 Vol.51 No.1

1993년부터 2015년까지 관측된 제주도 지하수 장기모니터링 관측정(N = 4,835)에서 수집된 지하수 수질자료(N = 21,568)를 기반으로 질산성질소의 시공간적 변동 특성을 평가하였다. 제주도 지하수의 질산성질소 농도의 중앙값은 2.5 mg/L로서 다른 국가나 대륙의 조사 결과에 비해 다소 높거나 유사한 것으로 나타났다. 또한 지하수 용도, 행정구역 및 고도 별로 유의한 차이를 보였다. 특히, 산간 지역에 비해 저지대 해안가에 위치한 농업 및 주거지역에서 농도가 높음을 확인하였다. Mann-Kendall 및 Sen’s slope 분석을 활용한 질산성질소 농도의 추세 분석 결과, 하류 저지 대에 비해 중산간지역에서의 질산성질소 농도 증가 경향이 뚜렷하였다. 제주도 내 토지 피복의 시계열 변화 특성과 결부 지어 보면, 중산간지역의 오염 증가 추세는 농업지역의 확장 등 인위적 활동 증가에 기인한 결과로 판단된다. 반면,기지정된 지하수자원특별관리구역에서는 전반적으로 질산성질소 농도의 감소 경향이 나타났는데, 이는 지하수 관리 측면에서 수질관리를 위한 적극적인 정책이 유효함을 시사한다. 본 연구에서는 제주도 지하수의 질산성질소 오염관리를 위한 적정 방안을 제안한다. The spatio-temporal variations of nitrate concentrations in groundwater of Jeju Island were evaluated by an analysis of time series groundwater quality data (N = 21,568) that were collected from regional groundwater monitoring (number of wells = 4,835) for up to 20 years between 1993 and 2015. The median concentration of NO 3 -N is 2.5 mg/L, which is slightly higher than those reported from regional surveys in other countries. Nitrate concentrations of groundwater in wells tend to significantly vary according to different water usage (of the well), administrative districts, and topographic elevations: nitrate level is higher in low-lying agricultural and residential areas than those in high mountainous areas. The Mann-Kendall trend test and Sen’s slope analysis show that nitrate concentration in mid-mountainous areas tends to increase, possibly due to the expansion of agricultural areas toward highland. On the other hand, nitrate concentrations in the Specially Designated Groundwater Quality Protection Zones show the temporally decreasing trend, which implies the efficiency of groundwater management actions in Jeju. Proper measures for sustainable groundwater quality management are suggested in this study.

미들박스 링크 비용을 고려한 라우팅 비용 변화에 대한 연구

권수경(Su-Kyung Kwon),김태준(Tae-June Kim),김정구(Jeong-Goo Kim),최윤호(Yoon-Ho Choi) 한국통신학회 2015 한국통신학회 학술대회논문집 Vol.2015 No.06

정헌 고인석 교수 정년퇴임기념호 / 경희한약개발연구소편 : 웅황의 연용이 흰쥐의 체동 , 비소축적 및 간기능에 미치는 영향

권창호 ( Chang Ho Kwon ),이경태 ( Kyung Tae Lee ) 경희대학교 동서약학연구소 1986 경희약대 논문집 Vol.14 No.-

폴리에스테르 織物의 熱傳導率과 初期最大熱流速에 관한 硏究

權五敬,曺扈絃 慶一大學校 1992 論文集 Vol.8 No.1

인간의 건강한 생활은 인체의 생산열량과 환경에 따른 에너지교환사이의 올바른 균형에 있으며 쾌적한 의생활을 영위하기 위해서는 생물학적 열방정식의 체내축적 열량을 0이 되도록 외계환경에 맞춘 여러가지 의복설계와 착용방법과 더불어 그의 소재가 되는 천에 대해서도 열적 특성을 규명할 필요가 있다. 특히 피복의 소재가 되는 직물은 섬유, 공기, 수분 등으로 구성된 복합체이고, 천의 열이동현상은 섬유, 공기, 수분 각각의 열전도율 및 섬유간이 복사, 대류 등이 조합된 복잡한 현상이다. 본 연구에서는 의복-열-환경에서의 열이동 특성을 규명하기 위한 기초연구로서 피�珦渶�많이 사용되는 폴리에스테르 직물의 열전도율과 피복착용시 온냉감과 깊은 관계가 있는 순간 접촉 열이동량인 초기열유속 최대값을 밝히고, 이들 특성치에 영향을 미치는 인자들간의 관련성을 규명하였다.

항공기 압력차단벽 손상허용설계를 위한 피로균열성장거동 해석

권정호,황경정 울산대학교 1999 공학연구논문집 Vol.30 No.1

압력차단벽 구조는 보강박판 구조형태로 이루어져 있으며 초기 설계과정에서 균열성장평가를 위한 응력강도해석에 유한요소해석법을 널리 사용하고 있으나 반복되는 계산작업이 매우 번거롭고 많은 시간이 소요되게 된다. 특히 압력차단벽은 내압을 효과적으로 감당하기 위하여 보통곡면 보강구조 형태로 설계되며 이러한 구조적 특성상 보강평판과는 달리 내압에 의해 표피구조에 이축응력이 발생하고 균열면을 따라 부풀어오름(bulging)현상이 발생되기 때문에 설계과정에서 손상허용해석이 까다롭게 된다. 이에 본 연구는 보강평판 해석에 적용한 바 있는 중첩법을 활용하여 압력차단벽 구조의 응력강도해석을 수행하고 유한요소해석 결과와 비교함으로써 중첩법의 활용 가능성을 검토하였다. 또한 내압차단벽의 손상허용 범위를 중앙보강재가 건재한 경우와 파손된 경우로 나누어 표피균열 끝단의 응력강도 해석 및 균열성장해석을 수행함으로써 손상허용성 확장에 따른 균열성장수명을 비교하였다. The fuselage pressure bulkhead is one of the important primary structures which should be designed satisfactorily damage tolerant. The calculation of fatigue crack growth in this study formed part of the preliminary analysis of damage tolerance design for the fuselage rear bulkhead of a medium civil transport. On the basis of superposition technique, stress intensity solution was derived for the cracked stiffened panel of the pressure bulkhead. The solution used is that of Poe for a built-up skin stiffener construction having a skin crack originating at a failed stiffener location. Both cases of intact and broken center stiffener were considered on the calculation of crack tip stress intensity factor. The only loading on the bulkhead is that of pressure, which is consisted of intermal cabin pressurization and external suction. The finite element analysis was also carried out for the stress intensity factor solution and its results were compared to that of closed form solution. The influence of center stiffener broken and biaxial stress state of skin, and also the effect of skin bulging due to internal pressure on crack growth behaviors, are also studied.

종이상자포장기의 개발에 관한 연구

권경우,권병철,류봉조,명태식,박찬호,이광원 한밭대학교 산업과학기술연구소 1994 논문집 Vol.2 No.-

The paper describes the development of a new automatic wrapping machine using paper films as it has not been used so far. The most of the automatic wrapping machine have used the vinyl film causing environmental pollution, the paper films, however, have a weak point in the aspect of high temperature adhesion. Therefore, the mechanism of the existing wrapping machine should be altered to prevent it from curling effect through the technical know-how. A new method and technology reducing the curling effect in the automnatic wrapping machine are demonstrated by using developed blower. Thus it is exqection and will decrease environmental pollution.

신경섬유종증 환자에게서 발생한 특발성 혈소판 감소증 1예

권세훈,김형호,강지인,장재혁,원경준,장재현,김상용,배학연 朝鮮大學校 附設 醫學硏究所 2006 The Medical Journal of Chosun University Vol.31 No.3

Idiopathic thrombocytopenic purpura is a disease characterized by thrombocytopenia in peripheral blood with positive anti-platelet antibody titer, no splenomegaly, the presence of megakarocyte. The causes of idiopathic thrombocytopenic purpura were usually related to autoimmune disease, infection and drugs. A 46-years-old man was admitted for evaluation of abdominal mass. He has many cafe-au-lait spots and nodules in almost skin for 30 years. Several years ago, he palpated mass in Lt. inguinal area. hIe has known the abdominal mass after checking abdomen CT in local clinic and visited our hospital for evaluation of abdominal mass. At admission, laboratory tests showed hemoglobin 12.5 g/dL, hematocrit 38.4 %, WBC 5,460 /㎣ (neutrophil 64.5%, lymphocyte 21.7%, monocyte 4.1%, eosinophil 5.4%), reticulocyte 0.7%, Platelet 52,000 /㎣, total protein 6.51 g/dL, albumin 3.80 g/dL, alkaline pohsphatase 161 IU/L, AST 110.7 IU/L, ALT 64.9 IU/L, total bilirubin 0.44 ㎎/dL, BUN 18.4 ㎎/dL, creatinine 0.79 ㎎/dL. Peripheral blood smear showed normocytic normochromic anemia, thrombocytopenia. Bone marrow aspiration smear showed normoplastic myeloid and erythroid precursor. The bone marrow biopsy section shows normocellular marrow (35%) for his age and mildly increasing myeloid cell lineage. We have experienced a case of idiopathic thrombocytopenic purpura in a patient being in neurofibromatosis and therefore reporting it with documentary records. 저자들은 신경섬유종증 환자에서 특별한 원인을 발견할 수 없었음에도 특발성 혈소판 감소증이 발생한 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

소아 조갑진균증에서 Itraconazole 주기요법의 치료효과

권경술,이재봉,진현우,오창근,장호선,임재영 대한의진균학회 2003 대한의진균학회지 Vol.8 No.4

대학병원 정신과 외래 환자의 약물순응도

권준수,신성웅,정영미,박경호 大韓神經精神醫學會 1997 신경정신의학 Vol.36 No.3

Objective : In view of the facts that education about diseases and realization of the need form drugs can improve compliance of the patients, we assessed the knowledge about drugs and medication compliance in psychiatric outpatients. In addition, we investigated factors that influence patients compliance to develop a model of medication compliance. Methods : One-hundred twelve male and eighty female psychiatric outpatient were asked about drug information using questionnaires. We developed a model that predicts medication compliance using chi square tests and multiple regression analysis. Results : More than a half of the patients knew at least one of the names and the effects of the drugs they had taken, but many of them didn't know the side effects of drugs. About eighty percent of the patients showed good compliance. Knowledge of side effects, belief in the benefits of drugs, and level of education could predict medication compliance reliably. Conclusions : Because education level could not be managed by physician, we could enhance compliance through education about drug side effects and benefits of medications.