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      • 나비目 終齡幼蟲의 血球에 關한 電顯的 硏究

        梁凞永,李鳳熙,鄭啓憲 순천향의과대학 1979 Journal of Soonchunhyang Medical Science Vol.2 No.1

        Morphological studies on larval hemocytes of varioius insects were performed in two ways of research. Ⅰ. Finestructures. An electron microscopical study on hemocytes of last instar larvae of 12 species of Lepidoptera was performed to observe cell types, structrues, raions, relationships and their origins. The results are as follows; 1) Prohemocytes are the smallest round cells with large numlesus, poorly developed cytoplasmic organelles and lower composition ratio, accounting for less than 5% of all cell types. Little remarked differences were revealed among prohemocytes of varous insects and they are thought to be the origins of other cell types. 2) Palsmatocytes are relatively large cells with higher composition ration, accounting for about 40%. They could be subdivided into two forms, oval and spindle. Oval cells are rich in cytoplasmic projections and organelles. But the spindle cells include few of them, while nearly all of the spindle cells possess some microtubules as their cytoskeletons. Some marked difference were revealed among different insects. The most exceptional structure was the fibrous bundles located in both nucleus and cytoplasm in Sericinus telamon Donovan. 3) Granular cells are characterized by various granules in cytoplasm, and by higher composition ration, accounting for about 50% of all numbers. They could be subdivided into three forms, oval, round and spindle with fully developed cytoplasmic organelles and Projections. Cellular fractions were often noticed to be enclosed by granular cells, indicating some activities of phagocytosis. Granular cells are the most pleomo rphous of all cell types, and the most exceptional structures are the bundles of microfibrils in Phalerodonta manleyi Leech. 4) Adipohemocyte were found only in Plodia interpunctella Hubner. They are somewhat oval with few cytoplamic projections and less developed organelles. Some granular cells were found to accumulate lipid droplets into the cytoplasm by trapping floating lipid droplets, adn the adipohemocytes are believed to be originated from formers. 5) Sperule cells were found nearly in all specimens and characterized by sperules composed of flocculent materials. They are round or oval with few cytoplasmic organelles. The most conspicuous spherulesof all sperule cells were radial arrangement of sperules in Adristyrannus amurensis Staudinger and presence of the specific granules in Mamestra illoba Butler. Young sperule cells resembled the granular cells and it is possible that they originate from granular cells. 6) Oenocytoids are large cells and were found nearly in all specimens. They could be subdivided into 3 types according to their inclusions, such as microfibrils, microtubules and none of them. Young Oenocytoids resemble the giant plasmatocytes, perhaps in dicating to be originated from the latters. Ⅱ. Composition ratios of cell types from mid 5-instar to late prepupa. Composition ratios of cell types were investigated in phalerodonta manley; Leech and could be summarized as follows; 1) Prohemocytes disappeared from the stage of early prepupa. 2) Plasmatocytes increased in number at mid stages of each inster and decreased nearing to pupal stage. 3) Granular cells reached peak at early prepupal stage. 4) Adipohemocytes heavily increased at late prepupal stage. 5) Sperule cells increased at late stage of each instar and reached peak at late prepupal stage. 6) Oenocytoids increased at late stage of each instar and reached peak at early prepupal stage.

      • 톡소포자충 배양시 약제투여에 따른 Toxoplasma P30 유전자의 발현

        이상걸,이영하,김계영,나영언,신대환 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

        To know the drugs effect on gene expression and its regulating mechanism by RT-PCR and southern blot hybridization assay based on detection of the P30 gene encoding the major surface antigen, P30 of Toxoplasma gondii and the DNA synthesis in T. gondii infected HeLa cells. The experiment was performed and the results were as follows. 1. The thymidine incooperation in DNA of HeLa cell and T. gondii infected HeLa cell reached peak level at 36 hours and suddenly decreased at 48 hours. The uracil incooperation in DNA of T. gondii reached peak level at 48 hours. 2. The decrease of P30 gene expression began from 24 hours after pyrimethamine treated and 36 hours after methotrexate treated in T. gondii. The results were some what different. Pyrimethamine was more sensitive and acted earlier to T. gondii than Methotrexate did. The decrease of P30 gene expression of T. gondii was regulated these drugs in dose dependent manner. 3. The effects of dibutyryl cyclic AMP on P30 gene expression increased peak at 24 hours and on contrary decreased at 60 hours after treatment in dose dependent manner, but at 10mM high concentration of dibutyryl cAMP, P30 gene expression was supressed. 4. Actinomycine D supressed the P30 gene expression of T. gondii in HeLa cell. As the results, we presume pyrimethamine acts more powerful and earlier to supresses P30 gene expression than methotrexate, and it is regulated at transcription level. The results of RT-PCR were in agreement with that of southern blot hybridization.

      • KCI등재
      • 어린이용 다기능 놀이기구 디자인 개발

        계재영,조원휘 대구보건대학 1997 대구보건대학 論文集 Vol.17 No.-

        Childhood is very important in one s physical and psychological development through the whole life. The development status of childhood exerts an influence upon physical and spiritual health of the future. Especially its development is dominated by environmental conditions. Unlike adults, children have fantastic, clear, and various sensibility. As they always express themselves following their inner desires, we should provide them with children s culture and environment which can satisfy with their desires and promote their intelligence and spirit. As we investigate carefully that how our children live under the rapid changes of modem society, what they want, and how they behave, express and feel, it s necessary to develop the diversified and reasonable play things fit for children s characteristics which can satisfy with their eyelight improving lack facilities and considering what the want, and also can help the development of their physique, language, emotion, acknowledgment and sociality. First, as a plaything to help children s creativity, social development and physical development in a small in a small area, we made it to be used variously. Second, we made it be controlled according to the height and length because children grow up rapidly in their height and weight. Third, as it s users are young. we made it to be assembled easily and mode it with hygienic and strong materials. Fourth. we improved the degree of children s color satisfaction and efficiency, providing various colors to consider their psychology. As they can experience disjointing, jointing, stability and rhythm through children s various playing desires and the basic type of things using the playthings designed like these, their world can be more rightful.

      • SCOPUSKCI등재
      • KCI등재
      • 영도지역의 컴퓨터 환경과 활용에 관한 연구

        계영희 고신대학교 영도발전연구소 2000 영도연구 Vol.2 No.-

        The purpose of this study is to investigate computer environments and residents' ability of using Internet in Youngdo-Gu(District), Busan. In this study, the survey was conducted by questionnaire from 210 residents in Youngdo-Gu (District). The answers to the questionnaire were analyzed on the time of computer used, the necessity of computer education, the ability of using Internet in each age group of 20's, 30's, and 40's. The results showed that there were significant differences among age groups between male and female. Further results were discussed in detail.

      • KCI등재

        외국인 노동자와 문화의 정치학: 개념과 논의

        박계영 대구대학교 다문화사회정책연구소 2014 현대사회와 다문화 Vol.4 No.2

        한국은 오랫동안 단일민족국가를 지향해 왔으나 최근 예상치 못했던 고령화와 저출산 그리고 급변해 가는 가족구조와 외국인 노동자 대거 유입 등의 대대적인 인구학적 변화를 겪게 되었다. 특히 외국인 노동자 유입으로 인해 한국사회는 급격히 다문화사회로 전환되었다. 본 논문은 종합적이고 체계적인 다문화주의 정책이 부재한 한국사회에서 적절한 정책을 위한 이론과 사례 및 외국 노동자 유입에 따른 사회변화들을 종합적으로 검토하고자 한다. 일찍부터 다문화주의 정책을 시행해 온 북유럽국가들의 이론과 사례를 '귀감(龜鑑)'으로 삼을 것을 제안한다. 이들 다문화국가들은 한국과 마찬가지로 민족의 동질성을 유지해 왔으나 이민은 물론 난민들을 대거 받아들이는 가운데 대규모 인구학적 변화를 겪게 되었다. 오랫동안 이민을 받아들였고 또한 진보주의에 입각하여 이들의 권익을 보호하는 정책을 펴 왔으나 근래 경제성장이 둔화되는 가운데 시민들의 반발이 심하게 되었다. 이들 국가의 다문화주의 정책의 성공과 실패 그리고 그저변의 이론적 배경을 분석해 보면 한국 같은 신흥 다문화주의 표방국가에서 배울 점이 많이 있다. 또한 외국 노동자 유입에 대한 개념적 이론적 분석과 관련된 정책변화도 검토하고자 한다. 한국의 경우 아직까지 다문화주의 정책이 결혼이주자에게만 한정되어 있어 이 기회에 외국 노동자층까지 확대시키기를 주장한다. 동시에 이민 및 이주자들에 관계된 다문화주의와 또는 대안적 이론들인 문화적 다양성과 사회적 다원론 등의 이론 및 정책적 배경에 대해서도 살펴본다. South Korea, long considered an ethnically homogenous country, faces unprecedented demographic changes: long life expectations, rock-bottom birth rates, changing family structures, and migration. Migration in particular posses challenges to the South Korean nation-state, as it did (and contines to do so) in European countries. These challenges have produced dabates about multiculturalism in South Korea. In this article, I map a conceptual landscape of the issues surrounding migration and multiculturalism in Korea, including the social changes precipitated by international migration. I place these issues in the context of past histories and present anxietied by doing a comparative analysis of Northern European experiments in multiculturalism. Like South Korea, Northern European countries were historically ethinically homogenous but faced demographic changes in the wake of international immigration. Although these countries initially welcomed immigrants and implemented liberal policies to protect their rights, recent economic downturns have generated a backlash against this embrace of multiculturalism. The successes and failures of these countries' multiculturalist policies have much to offer in mapping the conceptual landscape of a newly-branded multiculturalist society like South Korea (hereafter Korea). In addition to this comparative analysis. I will also critically examin conceptual and theoretical discussions on international migration and multiculturalism, together with an analysis of policy ternds. Past discussion on international migration has focused on foreign spouses and their families, but I will widen this focus to include transnational workers. This wider focus expands the disscussion beyond multiculturalism in order to examine other topics raised by a diverse society, including cultural diversity and social pluralism.

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