동양달팽이 Nesiohelix samarangae 소화관에서의 cellulase 활성에 대한 세포화학적 및 면역세포화학적 연구

정계헌,이용석,김은정 한국패류학회 1998 The Korean Journal of Malacology Vol.14 No.2

In order to observe the anticellulolytic localization in the epithelia of the digestive tract such as esophagus, crop, and intestine of a Korean land snail N. samarangae, a cytochemical method and a immunogold labelling method were applied. For the cytochemical study on the cellulase activity, Benedict reaction method applied. And for the immunocytochemical study, the rabbit serum immunoglobuins (IgG) was obtained from the rabbits injected with cellulase which was extracted from body fluid of the snail. The digestive tract tissues of N. samarangae were fixed with 4% paraformaldehyde and 2% OsO4 and embedded in Lowicryl K4M at -40$^{\circ}C$ under UV light (360 nm). The thin sections were loaded on the nickel grids and stained with the serum IgG and protein A-gold complex (particle size: 10 nm). Observations were undertaken with transmission electron microscope (Jeol, JEM-1010). The epithelium of the digestive tract was consisted of five types of cells. In the cytochemical study, the reaction products were found along the periphery of the vacuoles derived from the Bebedict reaction. In the immunocytochemical study, the protein-A gold particles were selectively labelled in Type 1, Type 3 and Type 4 cells in intestinal tissue. membranes of rER, in the surrounding cytoplasm of the rER and secretory granules, and in the apical cytoplasm of the cells. On the material being secreted from the apical cytoplasm was also labelled with the immunogold particles. The all results obtained throughtout present study suggest that the intestinal epithelium of the snail N. samarangae seretes cellulase as one of digestive enzymes.

昆蟲의 變態에 따른 神經組織의 微細構造的 硏究

鄭啓憲,李鳳熙 순천향대학교 1997 Journal of Soonchunhyang Medical Science Vol.3 No.3

나비目 終齡幼蟲의 血球에 關한 電顯的 硏究

梁凞永,李鳳熙,鄭啓憲 순천향의과대학 1979 Journal of Soonchunhyang Medical Science Vol.2 No.1

Morphological studies on larval hemocytes of varioius insects were performed in two ways of research. Ⅰ. Finestructures. An electron microscopical study on hemocytes of last instar larvae of 12 species of Lepidoptera was performed to observe cell types, structrues, raions, relationships and their origins. The results are as follows; 1) Prohemocytes are the smallest round cells with large numlesus, poorly developed cytoplasmic organelles and lower composition ratio, accounting for less than 5% of all cell types. Little remarked differences were revealed among prohemocytes of varous insects and they are thought to be the origins of other cell types. 2) Palsmatocytes are relatively large cells with higher composition ration, accounting for about 40%. They could be subdivided into two forms, oval and spindle. Oval cells are rich in cytoplasmic projections and organelles. But the spindle cells include few of them, while nearly all of the spindle cells possess some microtubules as their cytoskeletons. Some marked difference were revealed among different insects. The most exceptional structure was the fibrous bundles located in both nucleus and cytoplasm in Sericinus telamon Donovan. 3) Granular cells are characterized by various granules in cytoplasm, and by higher composition ration, accounting for about 50% of all numbers. They could be subdivided into three forms, oval, round and spindle with fully developed cytoplasmic organelles and Projections. Cellular fractions were often noticed to be enclosed by granular cells, indicating some activities of phagocytosis. Granular cells are the most pleomo rphous of all cell types, and the most exceptional structures are the bundles of microfibrils in Phalerodonta manleyi Leech. 4) Adipohemocyte were found only in Plodia interpunctella Hubner. They are somewhat oval with few cytoplamic projections and less developed organelles. Some granular cells were found to accumulate lipid droplets into the cytoplasm by trapping floating lipid droplets, adn the adipohemocytes are believed to be originated from formers. 5) Sperule cells were found nearly in all specimens and characterized by sperules composed of flocculent materials. They are round or oval with few cytoplasmic organelles. The most conspicuous spherulesof all sperule cells were radial arrangement of sperules in Adristyrannus amurensis Staudinger and presence of the specific granules in Mamestra illoba Butler. Young sperule cells resembled the granular cells and it is possible that they originate from granular cells. 6) Oenocytoids are large cells and were found nearly in all specimens. They could be subdivided into 3 types according to their inclusions, such as microfibrils, microtubules and none of them. Young Oenocytoids resemble the giant plasmatocytes, perhaps in dicating to be originated from the latters. Ⅱ. Composition ratios of cell types from mid 5-instar to late prepupa. Composition ratios of cell types were investigated in phalerodonta manley; Leech and could be summarized as follows; 1) Prohemocytes disappeared from the stage of early prepupa. 2) Plasmatocytes increased in number at mid stages of each inster and decreased nearing to pupal stage. 3) Granular cells reached peak at early prepupal stage. 4) Adipohemocytes heavily increased at late prepupal stage. 5) Sperule cells increased at late stage of each instar and reached peak at late prepupal stage. 6) Oenocytoids increased at late stage of each instar and reached peak at early prepupal stage.

쌍구흡충(Paramphistomum cervi)에서의 정자형성과정

정계헌,박종안 한국통합생물학회 1995 동물학회지 Vol.38 No.1

소의 위에 기생하는 쌍구흠충(Paromphistomum cewi)에서의 정자형성과정을 광학현미경과 투과전자현미경을 이용하여 관찰하였다 발생중인 모든 세포들은 중앙에 있는 세포질로부터 각 세포들로 이어지는 세포간교에 의하여 서로 연결되어 있어 분열과 분화의 동시성을 위한 영양계를 이루고 있었다 정원세포들은 4세포기까지이며 모두 기정막에 붙어 있었다. 일차정모세포는 8세포, 이차정모세포는 16세포가 영양계를 이루었고, 정세포는 32세포가 영양계를 이루었으며 이 상태에서 변태를 진행하였다 성숙한 정자의 머리는 나사모양이고 첨체는 형성되지 않았으며 2개의 축사를 가지고 있었다 축사의 단면에서 미소관의 배열상태는 9치 유형이었다.

糞便에서 檢出된 Escherichia coli의 抗菌劑 耐性에 關한 硏究

오영숙,정계헌 순천향대학교 1992 논문집 Vol.15 No.3

A total of 120 strains of Escherichia coli which were isolated from the stool specimens of the in-or out-patients a hospital located in Kyungki-Province was examined to find out their antibiotic resistances. The results obtained from the present study are as followings. 1. 107 strains(89.2%) out of total 120 isolates were resistant to Ampicillin. Different resistances were shown by drug such as 104 strains(86.7%) to Tetracycline, 84 strains (70.0%) to Kanamycin, 78 strains(65.0%) to Streptomycin, 75strains (62.5%) to Chloramphenicol, and 5 strains (4.2%) to Gentamicin. 2.40 strains (33.3%) were resistant to four drugs, and 32 strains (26.6%) were resistant to five drugs. 3.91 strains (75.8%) out of total 120 isolates were resistant to Ampicillin and Teracycline at the same time.

긴촌충(廣節裂頭條蟲) 感柒의 一例

李駿商,林漢鍾,鄭啓憲 순천향대학교 1980 논문집 Vol.3 No.3

韓國에서의 긴촌충(廣節裂頭條史) 感染例는 日制時 慶南地方 住民들의 大便檢査 증에 史卵이 觀索되었다는 報告가 있었고, 患者로부터 成忠을 얻어 報告한 例는 趙等(1971)이 報告한 1例 뿐이었다. 著者들은 14歲의 韓國少年으로부터 길이 685cm의 頭節이 없는 史體를 얻었다. 治療藥製는 Bithionol이었다. 忠體를 形態學論으로 檢討한 바 Diphyllobothrium latum(Linnaeus, 1758) Luhe, 1910으로 同定되었다. 患者는 그때까지 外國을 旅行한 經驗이 없었으며 市中에서 生鮮膾률 먹거나 약수터 또는 溪流의 물을 마신 記憶이 없고 더우기 뱀이나 개구리를 生食한 經驗은 전혀 없다고 한다. 다만 그는 本硏究室을 찾기 1年前 그의 父親을 따라 浦項으로 바다낚시를 간 일이 있으며 그곳에서 낚아 올린 농어( 魚)를 謄로 먹은 經驗이 있다고 하였다. 그러므로 本例의 感染源은 魚일 가능성이 많으며 分明한 것은 國內의 土着感染이란 점이다. , 國內에서 수시로 報告되는 sparganum症의 例로 보거나 中間宿主의 役割을 할 수 있다고 思料되는 甲穀類 및 魚類에 대한 報告와 韓國海洋의 魚類分布로 보아 韓國에서의 廣節裂頭條忠 感染率은 窓外로 높을지도 모른다. A case of Diphyllobothrium latum infection was recorded. A 14-year-old Korean boy was infected with this tape worm and treated with bithionol orally. An incomplete strobila without scolex and neck parts was collected. It was identified as D.latum on the basis of morpho1ogical characteristics. The most probab1e source of infection is autochthonous according to the past history of the patient. This is the second record of Diphyllo-bothrium latum infection in Korea which was verified with the recovery of the adult worm.

동양달팽이의 눈에 대한 해부학적 및 미세구조적 연구

정계헌,이현 한국패류학회 1994 The Korean Journal of Malacology Vol.10 No.1

各種織物販賣場 大氣內의 Formaldehyde 濃度調査

鄭啓憲 서울大學校 保健大學院 1968 公衆保健雜誌 Vol.5 No.2

This survey was conducted to determine the concentrations of formaldehyde (HCHO) in the atmosphere of the textile stores during the period of 1 Aug., 1968∼30 Sept., 1968 and another survey was carried out to find out the health conditions of the subjects who worked in the textile stores during this period. From the results of these studies, the following conclusions were obtained: 1. The average daily concentrations of HCHO in the atmosphere of the textile stores the synthetic fabrices stores without ventilators showed the highest (7.9ppm), and it was the lowest (0.9ppm) at the cotton fabrics stores. All other stores which did not deal with textiles was shown an average daily reading of 0.6 ppm. 2. The variations in the concentrations of HCHO with relation to the time of day were the highest from 3 to 4 o'clock in the afternoon, and were the lowest from 9 to 10 o'clock which was right after the stores were opened in the morning. 3. Almost 100% of subjects who answered the questionnaire reported symptoms of formaldehyde irritation. The highest rate (88.5%) of complaints of general symptoms was among women who worked in the synthetic fabrics stores and the lowest(52.9%) among men working in the clothing stores. 4. The percent of irritating symptoms which were reported as "sick at present" were as follow : 70.4%(the highest frequency rate) reported by women who worked in the synthetic fabrics stores and 12.6% (the lowest frequency rate) reported by men working in the general goods stores. The frequency rates of the general symptoms were as follow: 48.4% reported by women who worked in the synthetic fabrics stores and 12.6% reported by men working in the clothing stores. 5. The incubation period of the irritating symptoms of women who worked in the synthetic fabrics stores was the shortest, with 1.5 years and the longest, with 3.2 years to men who worked in the clothing stores. The incubation period of the general symptoms were almost same to all the subjects, between the periods 3∼4 years. 6. In the atmosphere of the textile markets, the concentrations of formaldehyde were the highest at the synthetic fabrics stores. The concentrations of formaldehyde were considerably over the maximum allowable concentration (M.A.C. : 5 ppm) in the atmosphere of all the synthetic fabrics stores and most of the silk fabrics stores. The relative rise in prevalence with the increased concentration of HCHO was more pronounced for the irritating symptoms and general symptoms. The incubation periods were relatively short at the textile stores which were under the high concentrations of formaldehyde. Under the same circumstances, women were more sensitive to HCHO than men. 7. For the prevention of the injurious effects resulting from exposures to HCHO, it is essential that its concentration in the atmospheric air of the textile stores be kept below 5 ppm, and that the formaldehyde-type resins should not be used in the manufacture of textiles. All synthetic fabrics should be sold in the synthetic fabrics stores only and the temperature in the air of the synthetic fabrics stores be kept 17∼20℃. The contaminated atmospheric air of the textile stores should be exchanged with fresh air. All workers working in the textile stores should be taken periodic medical examination and all persons showing sign of irritation should be exclued from further exposure until these conditions being arrested completely.

동양달팽이의 소화관에 대한 조직화학적 및 미세구조적 연구

정계헌,이용석 한국패류학회 1998 The Korean Journal of Malacology Vol.14 No.2

A histochemical and ultrastructural study on the epithelia of some selected digestive tracks such as esophagus, crop, intestine of a land snail N. samarangae was carried out during the period of June 1997 to may 1998. The epithelium of digestive tract are simple columnar epithelium and consisted of five types of columnar cells. Type 1 cell which is majority in number has a brush border with microvilli on the free surface of the cell and contains numerous secretory granules supposed to be neutral mucopolysaccharide. Type 2 cell, elongated conical in shape, is rarely found in the epithelium. This cell also has a brush border with microvilli on its free surface and contains well developed rough surfaced endoplasmic reticulum, Golgi bodies, and secretory granules in various electron densities. This cell seems to produce both of acid and neutral mucopolysaccharides. Type 3 cell, which is morphologically similar to the Type 1 cell, has microvilli and cilia on the free surface and exists in group only in the limited regions of the intestine. Type 4 cell, typical goblet cell containing secretory granules in high electron density. Type 5 cell rarely found in the digestive tract. This cell contain inconspicuous materials.

동양달팽이의 위에 대한 조직화학적 및 미세구조적 연구

정계헌,이용석 한국패류학회 1997 The Korean Journal of Malacology Vol.13 No.2

A histochemical and ultrastructural study on the stomach epithelium of a land snail Nesiohelix samarangae was carried out during the period of June 1996 to May 1997. The stomach epithelium is simple columnar dpithelium and is consisted of three types of columnar cells, Type 1 cell which is majority in number has a brush border with microvilli on the free surface of the cell and contains numerous secretory granules supposed to be neutral mucopolysaccharide. Type 2 cell, elongated conical in shape. is rarely found in the dpithelium. This cell also has a brush border with microvilli on its free surface and contains well developed rough surfaced endoplasmic reticulum, Golgi bodies, and secretory granules in various electron densities. This cell seems to produce both of acid and neutral mucopolysaccharides. Type 3 cell, which is morphologically similar to the Type 1 cell, has microvili and cilia on the free surface and exists in group only in the limited regions of the stomach.