서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Hypoxia-Responsive MicroRNA-101 Promotes Angiogenesis <i>via</i> Heme Oxygenase-1/Vascular Endothelial Growth Factor Axis by Targeting Cullin 3

Kim, Ji-Hee,Lee, Kwang-Soon,Lee, Dong-Keon,Kim, Joohwan,Kwak, Su-Nam,Ha, Kwon-Soo,Choe, Jongseon,Won, Moo-Ho,Cho, Byung-Ryul,Jeoung, Dooil,Lee, Hansoo,Kwon, Young-Guen,Kim, Young-Myeong Mary Ann Liebert 2014 Antioxidants & redox signaling Vol.21 No.18

<P>Aims: Hypoxia induces expression of various genes and microRNAs (miRs) that regulate angiogenesis and vascular function. In this study, we investigated a new functional role of new hypoxia-responsive miR-101 in angiogenesis and its underlying mechanism for regulating heme oxygenase-1 (HO-1) and vascular endothelial growth factor (VEGF) expression. Results: We found that hypoxia induced miR-101, which binds to the 3 ' untranslated region of cullin 3 (Cul3) and stabilizes nuclear factor erythroid-derived 2-related factor 2 (Nrf2) via inhibition of the proteasomal degradation pathway. miR-101 overexpression promoted Nrf2 nuclear accumulation, which was accompanied with increases in HO-1 induction, VEGF expression, and endothelial nitric oxide synthase (eNOS)-derived nitric oxide (NO) production. The elevated NO-induced S-nitrosylation of Kelch-like ECH-associated protein 1 and subsequent induction of Nrf2-dependent HO-1 lead to further elevation of VEGF production via a positive feedback loop between the Nrf2/HO-1 and VEGF/eNOS axes. Moreover, miR-101 promoted angiogenic signals and angiogenesis both in vitro and in vivo, and these events were attenuated by inhibiting the biological activity of HO-1, VEGF, or eNOS. Moreover, these effects were also observed in aortic rings from HO-1(+/-) and eNOS(-/-) mice. Local overexpression of miR-101 improved therapeutic angiogenesis and perfusion recovery in the ischemic mouse hindlimb, whereas antagomiR-101 diminished regional blood flow. Innovation: Hypoxia-responsive miR-101 stimulates angiogenesis by activating the HO-1/VEGF/eNOS axis via Cul3 targeting. Thus, miR-101 is a novel angiomir. Conclusion: Our results provide new mechanistic insights into a functional role of miR-101 as a potential therapeutic target in angiogenesis and vascular remodeling. Antioxid. Redox Signal. 21, 2469-2482.</P>

D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

곽미선,이승구,정상철,서승현,이재흥,전영중,김영호,성문희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two therrnophiles, Bacillus sp. LK-I and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-0l, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

자폐장애 환자에서 FMR-1 유전 삼염기 반복의 분자생물학적 분석

곽호순,정철호,전효진,장은진,김희철,김정범,박영남 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.1

연구목적 : 자폐장애의 원인을 유전학적으로 규명하려는 연구가 시도되고 있으며, 그 중 fragile-X 증후군과의 연관성에 대한 연구가 활발히 진행되고 있다. fragile-X 염색체(Xq27.3)는 세포유전학적 방법으로 증명할 수 있으나 검사에 많은 제약과 단점이 있으므로, 본 연구에서는 보다 신뢰성이 높은 분자 생물학적 방법으로 FMR-1 유전자내 CGG 삼염기 반복부위를 분석하여 자폐장애와 fragile-X 증후군의 연관성을 규명하고자 하였다. 방법 : 자폐장애 환아(99명)와 정상대조군(8명)을 대상으로 FMR-1 유전자의 CGG 반복배열 부위를 sense와 antisense primer를 이용하여 PCR법으로 분석하엿으며, 동시에 세포유전학적 검사도 시행하였다. PCR 분석에서 CGG 반복수가 50 이상인 경우에 대해서는 StB12.3 혹은 Pfxa3 probe를 이용한 Sourthern blot hybridization으로 확인하였다. 결과 : FMR-1 유전자의 CGG 반복배열에 대한 PCR 분석 결과 CGG 삼염기의 반복배열의 수는 자폐장애 환자군과 정상대조군 사이에 통계적으로 유의한 차이가 없었다(p=0.207). 자폐장애 환자에서 CGG 반복수가 50회 이상인 조기변이(premutation) 환자가 2명 있었으나 Sourthern blot hybridization 결과 완전변이(full mutation)로 판정할 수 있는 경우는 없었다. 세포유전학적 검사에서 환자군 모두에서 정상 핵형을 나타내었으며 fragile-X 염색체는 확인되지 않았다. 결론 : 이상의 결과에서 자페장애 환자가 FMR-1 유전자의 CGG 삼염기 반복부위 이상, 즉 fragile-X 염색체 이상을 동반하지 않았음을 증명할 수 있었다. 이는 fragile-X 증후군을 자�장애의 직접적인 원인이라고 보기에는 어려움이 있음을 시사한다. Objectives : There has been a rapid expansion of studies aimed at elucidating the genetic basis of autistic disorder, especially it's relationship to fragile-X syndrome. The detection of fragile X chromosome(Xq27.3) by cytogenetic analysis has revealed many difficulties in testing. Therefore, to explore the relationship between autistic disorder and fragile X syndrome, this study administered molecular biologic methods which examined an unstable CGG repeat within the fragile X mental retardation-1(FMR-1) gene. Methods : Ninety nine autistic children and eight normal control children were tested. The number of CGG repeats within FMR-1 gene was measured after amplification by PCR, and cytogenetic analysis was also carried out to detect fragile site Xq27.3. Sourthern blot hybridization, using StB12.3 and/or Pfxa3 probe, was done for the patients showing expansion of more than 50 CGG repeats(premutation). Results : All but two autistic patients had no expansion in CGG repeats by PCR and there was no significant statistical difference in number of CGG repeat in comparison with normal control. Two autistic patients, considered as premutation by PCR analysis, had no full mutation or premutation by Southern blot hybridization. All autistic children tested did not have any abnormal karyotype or fragile site Xq27.3. Conclusions : These results suggest that autistic patients may not have abnormality in FMR-1 gene or abnormal expansion in CGG repeat. In conclusion, fragile X syndrome may not be antecedent of autistic disorder. KEY WORDS : Autistic disorder·FMR-1 gene trinucleotide repeats·Molecular biologic analysis.

고속 주행 모드에서 자동차 디퓨저의 스트레이크 개수와 배치에 따른 공기저항 유동해석

이진호,박성하,최동석,한영진,곽나영,이상욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

This study proposes an optimized diffuser strake design that can improve driving performance for cars in high-speed driving mode. The speed of the vehicle is set to 160km/h, and the number of strake is set to 4, 6 and 8, and the angle of the diffuser is set to 4, 5 and 6 degrees. The arrangement of the strake is centered, edged and equally arranged. The optimized diffuser design induced with the Ansys Fluent analysis shows a drag coefficient of 16% less than the basic diffuser-free model at a diffuser angle of 6 degree, 8 strakes, and equal arrangement.

종합정보통신망을 통한 제주지역 농산물 유통정보모델 구축에 관한 연구

곽호영,이상준,김장형 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

In this paper, we proposed a total information system model for cheju farming industry. The purpose of the model is to provide the useful informations and services about cheju farmings. For this purpose, we analized the various types of the cheju farming system. And analized the distribution channels of the agricultural products. And surveyed the kind of agricultural informations needed for the peasants. Using the surveyed data, we designed a computer information system model which is conceptually attached on the ISDN.

pH 5.5에서 재광화 용액의 포화도 변화가 인공 탈회된 법랑질의 재광화에 미치는 영향

곽영준,김의성,박성호,공형규,이윤,이찬영 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.5

초기 법랑질 우식의 경우 주위의 pH. 유기산의 종류 및 농도,치아의 무기질 및 유기질의 화학적 조성,불소의 농도,용액의 포화도등에 의해 영향을 받는다고 하였다. 본 연구에서는 lactic acid를 이용하여 법랑질을 인공 탈회시키고, pH 5.5인 포화도가 다른 세 가지 재광화 용액 (포화도 0.25,0.30, 0.35)으로 12일간 처리한 후,편광 현미경에서 얻은 상에서 탈회 깊이의 변화,우식 표면층 폭의 변화,무기질의 양적 변화를 Photoshop, Image pro plus, Scion Image, Excel을 이용하여 비교,분석하였다. 1. pH 5.5 에서 재광화 용액의 포화도가 증가할수록 우식 표면층의 폭은 증가하였다. 2. pH 5.5 에서 l 군의 경우 우식 부위에 전반적으로 재광화가 일어났으나,3군의 경우 우식 표면층에서만 재광화의 양상이 뚜렷하게 나타났고, 2군에서는 1군과 3군의 중간적인 양상이 나타났다. 한편,3군의 경우 1, 2 군보다 우식 표면층의 폭이 유의하게 증가하였다. 결론적으로 본 실험의 조건에서 포화도가 가장 낮았던 군에서는 우식 표면층에서 우식 섬부까지 전반적으로 재광화가 일어난 반면,포화도가 높아질수록 우식 표면층의 폭은 증가하였으나 우식 심부에서는 큰 변화를 보이지 않는 양상이 나타났다. The purpose of this study is to observe and compare the remineralization tendencies of artificially demineralized enamel by remineralization solutions of different degree of saturations at pH 5.5, using a polarizing microscope and computer programs (Photoshop, Image pro plus, Scion Image, Excel). For this study, 36 sound permanent teeth with no signs of demineralization, cracks, or dental restorations were used. The specimens were immersed in lactic acid demineralization solution for 3 days in order to produce dental caries artificially that consist of surface and subsurface lesions. Each of 9 or 10 specimens was immersed in pH 5.5 lactic acid buffered remineralization solution of three different degrees of saturation (0.25, 0.30, 0.35) for 12 days. After the demineralization and remineralization, images were taken by a polarizing microscope (× 100). The results were obtained by observing images of the specimens, and using computer programs, the density of caries lesions were determined. In conclusion, in the group with the lowest degree of saturation, remineralization occurred thoroughly from the surface to the subsurface lesion, whereas in the groups with greater degree of saturation showed no significant change in the subsurface lesion, although there was corresponding increase in the remineralization width on the surface zones.

컴파일러 최적화를 위한 코드 스케쥴링

곽호영,한정현 제주대학교 1992 논문집 Vol.35 No.-

This paper deals with instruction scheduling, one area of compiler optimization. This assures the correctness of program execution during occuring the interlock and reduces the execution time in pipelined processor. DAG(Directed Acyclic Graph) is used to represent the problem and is proven to be pretty convinient to represent the various Kinds of serialization constraints. Gibbon's scheduling algorithm with O(n²) is introdued to schedule the instruction from DAG. Three heuristics and some assumptions are explained to prevent the problem to be O(n⁴). Furthermore, the impact on another phase in compiler is discused. Actually this scheduling algorithm assumes to perform the instruction scheduling after register allocation. More work relating to this area will be the relationship beween two phases-register allocation and code schduling.

中心壓軸力을 받는 高强度 鐵筋콘크리트 기둥의 耐力과 延性에 關한 실험적 硏究

곽노현,이영호,김남주,이경종,정헌수 중앙대학교 기술과학연구소 1997 기술과학연구소 논문집 Vol.27 No.-

A study on the behavior of short reinforced concrete columns was experimently described. Twenty-four concrete columns, with 20cm square and 60cm height, containing 8 longitudinal steel bars and different the amount of ties, spacing and concrete strengths, were subjected to concentric load by failure. Test results indicated that 1) It is incompatible in high-strength concrete to arrange stirrups to the AIK code of D/2 throughout the example of 10cm stirrup spacing following AIK codes. 2) As the amount of stirrup increased, the strength and ductily of reinforced concrete column improved. 3) As the concrete strength is high, the effect of the was reduced. 4) If strength enhancement factors increased, strain at maximum load also does and ductility ratios does almost linearliary. 5) The rate of strength enhancement factor does well with the modified kent & Park's theory. 6) As to the result of calculating the effective confined area which is suggested in Sheikh & Uzumeri's theory, the middle vertical steel is effective, only if the stirrups are arranged with spacing of 3cm.

全北地方의 藥用作物 재배 및 生産現況

郭晙洙,金昌洙,宋英恩,金柱,朴珍華,韓宗鉉,宋昊俊 圓光大學校 韓醫學硏究所 1997 원광한의학 Vol.7 No.1

본격적인 지방화 시대를 맞이하면서 지역특화작물의 개발과 농가소득증대를 위한 새로운 기술개발이 절실한 이때 주요 소득원작물의 하나인 약용작물의 재배 및 생산현황을 파악하여 지역특성에 맞는 새로운 작목개발 및 정책 입안 자료로 활용하고자 조사분석한 결과는 다음과 같다. 1. 생산량을 기준으로한 전북지방의 주요 약용작물을 특화계수 분석결과 총 31개작목 중 독활, 백지, 양유, 오미자, 지황 등은 핵심품목으로 길경, 두층, 방풍, 작약, 천마 등은 주산품목으로 조사되었으며 강활 등 21개 작목은 비특화 작물로 분석되었다. 재배면적을 기준으로 한결과는 독활, 백지, 양유, 지황 등이 핵심품목으로 길경, 두층, 목단, 방풍, 백출, 오미자, 의이인, 작약 등은 주산품목으로 강활 등 19개 품목은 비특화 작물로 분석되었다. 2. 주요 약용작물 재배 계획면적 조사에서는 당귀, 두층, 오미자, 독활, 천궁 등이 계획 대비 실제 재배면적의 차이가 큰 작목으로서 이들은 일반적으로 상품가격의 영향을 많이 받는 것으로 분석되었다. 3. '96 재배면적 조사 결과 전북지방의 주요 약용작물 재배 계획 면적은 총 1,692.7ha였으며 이중 독활이 426ha로 가장 많았고, 길경(283ha), 양유(179ha), 작약(165ha), 두층(154ha), 당귀(113ha), 오미자 의이인, 지황, 산수유, 천궁, 방풍 등의 순이었으며 기타작목으로 백지, 황기, 백하수오 등 28작목이 조사되었다. With the beginning of the new localization era, epoch-making techniques in local agriculture are needed to improve farm income and devlop a locally specialized crops. On the basis of these trends in local agriculture, the status quo of production in medicinal plants which are one of the major income crops were surveyed and analyzed the data for developing a new crops and drafting a policy. The major results are as follows; 1. The results analyzed by Location Quotient of production rate in Chonbuk were classified three catagories. 5 items including Aralia continentalis K_ITAGAWA among 31 items were so rted out as a core crops, another 5 items including Platycodon grandiflotum(J_ACQ.) A.DC. were as a main crops and other 21 items were as a non-specialized crops. Analyzed by cultivation area, 4 items were sorted out as a core crops, another 8 items were as a main crops and the other 19 items were as a non-specialized crops. 2. In comparison with the gap between intended cultivation area and real cultivation area, 5 items including Eucommia ulmoides OLIVER were showed more larger than the others. The above mentioned items were assumed to be more influenced by the price. 3. Farmer's intended cultivation areas of the major medidinal plants in 1996 were surveyed totals of 1.692 ha in Chonbuk. Among them, Aralia corolata was surveyed as the top totals of 426 ha. in area.