Effect of gomisin A on osteoblast differentiation in high glucose-mediated oxidative stress

Takanche, Jyoti Shrestha,Kim, Ji-Eun,Han, Sin-Hee,Yi, Ho-Keun Elsevier 2020 Phytomedicine Vol.66 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Gomisin A is a lignan isolated from the hexane of <I>Schisandra chinensis</I> fruit extract with antioxidant properties. Oxidative stress mediated by high glucose is one of the major complications of diabetes mellitus.</P> <P><B>Purpose</B></P> <P>This study investigates the role of gomisin A in osteoblast differentiation under high glucose-induced oxidative stress in MC3T3 E1 cells and determines its relationship with heme oxygenase-1 (HO-1) and mitochondrial biogenesis.</P> <P><B>Methods</B></P> <P>MC3T3 E1 cells were treated by gomisin A following induced by high glucose levels and glucose oxidase to investigate the inhibitory effect of gomisin A against high glucose oxidative stress. Western blot analysis, alizarin red staining, alkaline phosphatase (ALP) activity, analysis of reactive oxygen species (ROS) and confocal microscopy were used to determine mitochondrial biogenesis, oxidative stress, osteoblast differentiation and mineralization. To analyze the role of HO-1, the MC3T3 E1 cells were treated with the HO-1 inhibitor zinc protoporphyrin IX (ZnPP).</P> <P><B>Results</B></P> <P>Gomisin A enhanced the expression of HO-1, increased mitochondrial biogenesis factors (peroxisome proliferator-activated receptor gamma coactivator 1–alpha, nuclear respiratory factor-1, and mitochondrial transcription factor A), antioxidant enzymes (copper-zinc superoxide dismutases and manganese superoxide dismutase), osteoblast differentiation molecules (bone morphogenic protein-2/7, osteoprotegerin and Runt-related transcription factor-2) and mineralization by upregulation of ALP and alizarin red staining, which were decreased by ZnPP and high glucose oxidative stress. Similarly, gomisin A inhibited ROS which was increased by ZnPP and the high glucose-mediated oxidative stress.</P> <P><B>Conclusions</B></P> <P>The findings demonstrated the antioxidative effects of gomisin A, and its role in mitochondrial biogenesis and osteoblast differentiation. It potentially regulated osteoblast differentiation under high glucose-induced oxidative stress <I>via</I> upregulation of HO-1 and maintenance of mitochondrial homeostasis. Thus, gomisin A may represent a potential therapeutic agent for prevention of bone fragility fractures and implant failure triggered by diabetes.</P> <P><B>Graphical abstarct</B></P> <P>[DISPLAY OMISSION]</P>

LPS로 유도된 Human Dental Pulp Cells에서 Schisandrin C 의 항염증 효과

Jyoti Shrestha Takanche,Sang Won Lee,Yae Jin Kim,Young Hee Lee,Jeong Seok Kim,Ji Eun Kim,Ho Keun Yi,Sin Hee Han 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10

Background : Tooth vitality is reflected by the health of dental pulp. Schisandrin C is a natural compound extracted from the fruit of Schisandra chinensis which has anti-inflammatory and anti-oxidant properties. The role of Schisandrin C on human dental pulp cells (HDPCs) has not been studied yet. This study examined the properties of Schisandrin C as an anti-inflammatory and anti-oxidant compound, and whether its characteristics promote mitochondrial biogenesis in HDPCs. Methods and Results : HDPCs were extracted from fresh third molars and cultured. Reactive oxidative stress (ROS) and nitric oxide (NO) formation were analyzed by a Muse cell analyzer. Western blotting and gelatin zymography were used to identify the presence of anti-oxidants, as well as inflammatory and mitochondrial biogenesis. Confocal microscopy was used for the detection of mitochondrial activity. Schisandrin C inhibited lipopolysaccharide (LPS)-stimulated inflammatory molecules; intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), matrix metalloproteinase-2 and -9 (MMP-2/9), NO production, ROS formation and the mitogen-activated protein (MAPK) pathway through minimizing the nuclear factor kappa B (NF-kB) translocation. Schisandrin C increased the expression of superoxide dismutase (SOD) enzymes as well as heme oxygenase-1 (HO-1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a) through the phosphorylated-protein kinase B (p-AKT) and nuclear factor erythroid 2-related factor-2 (Nrf-2) pathways. The anti-inflammatory and anti-oxidant properties of Schisandrin C promoted mitochondrial biogenesis. Conclusions : These results suggest that Schisandrin C may be used as an anti-inflammatory compound to reduce oral inflammation such as pulpitis.

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

한국인 여성의 자궁경부암에서 PCR을 이용한 Human papillomavirus 아형의 검출에 관한 연구

김향우,전호종,김윤신,장원재,이미숙,이미자 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The polymerase chain reaction (PCR) was used for detection of human papillomavirus (HPV) in paraffin-embedded tissue. Tissues stored as paraffin blocks were a potential source of DNA for retrospective clinicogenetic analysis. Sections in 6 ㎛ (three or five) thick cut from paraffin blocks of 29 uterine cervix dysplasia, 34 uterine cervix carcinoma in situ, 42 uterine cervix squamous cell carcinoma and 10 uterine cervix metaplasia samples were deparaffinized and incubated at 37℃ for 48hrs. in the lysis solution. Following organic extraction and alcohol precipitation, and extracted DNAs were identified by gel electrophoresis. These DNAs carried out PCR. After repeated cycles of specific oligonucleotideprimed extension of viral DNA with thermophilic DNA polymerase. the type of HPV present was then determined on the basis of the size of the ethidium-bromide stained band visible after agarose gel electrophoresis : for HPV 6 is 263 bp. for HPV 11 is 144 bp, for HPV 16 is 601 bp. and for HPV 18 is 360 bp. This study detected HPV DNA in four cases. which broke down to HPV 16 in two case of uterine cervix carcinoma in situ and two case of uterine cervix squamous cell carcinoma, no HPV type in the remainder. Histologically, HPV types 6, 11 are mainly detected in benign genital warts and lowgrade cervical dysplasias, squamous cell carcinoma frequently contained HPV 16, whereas, HPV 18 was present in adenocarcinoma, adenosquamous cell carcinoma and small cell carcinoma of the cervix. These results indicate that cervical cancers might be clinically influenced by the differences in sutible of the infecting HPV.

유방에서 기원된 전이성 췌장암종 1예 : A case report

김윤신,이미숙,장원재,임성철,기근홍,전호종,서재홍 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

Pancreas is known for the unfavorable soil to the metastatic carcinoma, and pancreatic metastasis is achieved after systemic dissemination was developed. The primary tumors of metastatic pancreatic carcinoma are breast, lung, Iymphoma, malignant melanoma, leukemia, stomach. and colon. The breast carcinoma is the commonest tumor to metastasize to pancreas and both tumors are related to estrogen receptor for tumor growth. In the present case, tumor cells had small round nucleus and showed indian file infltrating pattern and lack of cohesion, which are seen in infiltrative breast carcinoma. The patient was underwent radical mastectomy due to infiltrative ductal carcinoma three years ago. And also the tumor cells showed nuclear reactivity to immunostaining for estrogen receptor In combination of the histopathologic. immunohistochemical, and her past history, we concluded that breast carcinoma was metastasized to pancreas, and we report a case of metastatic pancreatic carcinoma from breast with review of literatures.

역상 미셀용액에 가용화된 acetanilide, p-diacetylbenzene 및 tetrabromo-p-quinone의 미세환경의 성질

金信局,朴哲浩,李相佐,高正秀 木浦大學校 基礎科學硏究所 1995 基礎科學硏究誌 Vol.13 No.-

Solubilization of some carbonyl compounds such as acetanilide, p-diacetylbenzene and tetrabromo-p-quinone was investigated in a variety of reversed micellar solutions by UV spectrophotometric method. In the reversed micellar solutions used cyclohexane as a solvent, the microenvironmental polarity of these carbonyl compounds decreased as DAP> DAB> DAC> DAL By adding water into the reversed micellar solution, the polarity of the site for the solibilizate became higher.

급성 운동 신경 축삭성 신경병증과 동반된 기관지내 이물 1예

조영신,전웅,이호성,최재성,나주옥,서기현,김용훈 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

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CFS로 보수된 RC보의 파괴에서 사전균열의 영향

김충호,고신웅 경성대학교 공학기술연구소 2005 공학기술연구지 Vol.12 No.-

This investigation performed monotonic incremental loading test and repeating loading test of the beams repaired with Carbon Fiber Sheet(CFS) after occurring the crack corresponding to 0.75P_(y) representing the damages. Experiments confirmed that growth of the special pre-crack under the loading point was a direct cause of a delamination of the CFS and it led a failure of the beams. From the test results, it is concluded that the failure of beams repaired by CFS will occur at pre-crack under the loading point.

비침습적 검사를 이용한 수술후 하지 심부 정맥 혈전증의 감시

김영욱,정한준,윤봉호,김신윤,이상국,변경환,김태헌 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

Deep vein thrombosis is recognized as a common complication in surgical patients in western countries especially in patients with high risk factors. The purposes of this study were to detect leg DVTs in early postoperative period by non-invasive surveillance and to analyze the risk factors of DVT. One hundred seventy one patients who underwent major operations(67 curative resection of colorectal cancer, 64 total hip replacement, 38 femur operation for fracture, and 2 colon resections for benign colon disease) were included for the prospective surveillance of leg DVT within 2 weeks after the operations. For the surveillance of leg DVT, strain gauge plethysmography(SPG) and Duplex scanning of both legs were completed for all patients except 10 patients. These 10 patients were examined in only one leg. The patients with past history of leg DVT or under prophylactic anticoagulant therapy were excluded from this study. To determine the risk factors related with leg DVT formation, age and sex of the patients, indication of surgery or surgical procedures, duration of operation, position during the operation, duration of postoperative immobilization, and preoperative serum level of antithrombin III (AT- III) were analyzed using Chi-square test. After performing SPG of 342 legs, 38 legs, showed abnormal on venous outflow/venous capacitance discriminant line chart, and 13 legs of 12 patients showed the finding(s) suggesting DVT on duplex scanning. Among the patients with abnormal duplex findings, 7 limbs(53.8%) were symptomatic, but the remaining 6 legs were silent. In 6 (15.8%) patients of 36 femur operations, 3(4.7%) of 64 total hip replacements and 3(4.5%) of 67 curative resections of colorectal cancer developed DVT by duplex scanning in the iliac(5), femoral-popliteal(6), and isolated calf(2) veins. After analyzing the risk factors, we could not find any statistically significant(p<0.05) factor related with leg DVT.

디스크 배열의 최적 불록크기에 관한 연구

김신택,강창수,전창호 한양대학교 공학기술연구소 1994 공학기술논문집 Vol.3 No.1

Parallel, hence high speed, data transfer is possible with disk arrays on which files are interleaved on many disks. For disk arrays where data are interleaved in block units, the size of a block is a major performance parameter. Therefore it is important to find an optimal block size in order to maximize the bandwidth of disk arrays. In this paper, we analyze the effects of workload, disk parameters, and the number of disks on the block size. Based on the analysis, we present a method to find an optimal block size for a disk array.