신규 방사성 항암제 DW-166HC 의 소핵시험

문은이(Eun Yi Moon),이진(Jin Lee),이원용(Won Yong Lee),최청하(Chung Ha Choi),이덕근(Dug Keun Lee),유제만(Jei Man Ryu),정용호(Yong Ho Chung),윤성준(Sung June Yoon),박경배(Kyung Bae Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

DW-166HC (^(166)Holmium (^(166)Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumorigenic spectrum, especially against human hepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous arid intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I (^(165)Ho-Chitosan complex : cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC (^(166)Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr arid 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative controi group but there was significant difference of this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

Schisandrin C enhances odontoblastic differentiation through autophagy and mitochondrial biogenesis in human dental pulp cells

Takanche, Jyoti Shrestha,Kim, Jeong-Seok,Kim, Ji-Eun,Han, S-H.,Yi, Ho-Keun Elsevier 2018 Archives of oral biology Vol.88 No.-

<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>To investigate the role of Schisandrin C in odontoblastic differentiation, and its relations between autophagy and mitochondrial biogenesis in human dental pulp cells (HPDCs).</P> <P><B>Design</B></P> <P>Fresh third molars were used, and cultured for HDPCs. Western blotting technique, Alizarin red S staining, alkaline phosphatase (ALP) activity, and confocal microscopy were used to detect autophagy, mitochondrial biogenesis, and odontoblastic differentiation. To understand the mechanism of Schisandrin C, the HDPCs were treated with lipopolysaccharide (LPS), autophagy and heme oxygenase-1 (HO-1) inhibitors: 3-Methyladenine (3-MA) and Zinc protoporphyrin IX (ZnPP), respectively.</P> <P><B>Results</B></P> <P>LPS decreased the expression of autophagy molecules [autophagy protein 5 (ATG-5), beclin-1, and microtubule-associated protein 1A/1B light chain 3 (LC3-I/II)] and mitochondrial biogenesis molecules [heme oxygenase-1 (HO-1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α)], and disrupted odontoblastic differentiation. The down-regulation of autophagy and mitochondrial biogenesis with 3-MA and ZnPP inhibited odontoblastic differentiation. However, Schisandrin C restored the expression of all the above molecules, even with LPS and inhibitor treatment. This result demonstrates that autophagy and mitochondrial biogenesis plays an essential role in odontoblastic differentiation, and Schisandrin C activates these systems to promote odontoblastic differentiation of HDPCs.</P> <P><B>Conclusion</B></P> <P>Schisandrin C has potential characters to regulate odontoblastic differentiation, and may be recommended for use as a compound for pulp homeostasis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Autophagy and mitochondrial biogenesis are linked with odontoblastic differentiation. </LI> <LI> Schisandrin C promotes odontoblastic differentiation in HDPCs. </LI> <LI> It mediated this function via mitochondrial biogenesis and autophagy. </LI> </UL> </P>

Effect of gomisin A on osteoblast differentiation in high glucose-mediated oxidative stress

Takanche, Jyoti Shrestha,Kim, Ji-Eun,Han, Sin-Hee,Yi, Ho-Keun Elsevier 2020 Phytomedicine Vol.66 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Gomisin A is a lignan isolated from the hexane of <I>Schisandra chinensis</I> fruit extract with antioxidant properties. Oxidative stress mediated by high glucose is one of the major complications of diabetes mellitus.</P> <P><B>Purpose</B></P> <P>This study investigates the role of gomisin A in osteoblast differentiation under high glucose-induced oxidative stress in MC3T3 E1 cells and determines its relationship with heme oxygenase-1 (HO-1) and mitochondrial biogenesis.</P> <P><B>Methods</B></P> <P>MC3T3 E1 cells were treated by gomisin A following induced by high glucose levels and glucose oxidase to investigate the inhibitory effect of gomisin A against high glucose oxidative stress. Western blot analysis, alizarin red staining, alkaline phosphatase (ALP) activity, analysis of reactive oxygen species (ROS) and confocal microscopy were used to determine mitochondrial biogenesis, oxidative stress, osteoblast differentiation and mineralization. To analyze the role of HO-1, the MC3T3 E1 cells were treated with the HO-1 inhibitor zinc protoporphyrin IX (ZnPP).</P> <P><B>Results</B></P> <P>Gomisin A enhanced the expression of HO-1, increased mitochondrial biogenesis factors (peroxisome proliferator-activated receptor gamma coactivator 1–alpha, nuclear respiratory factor-1, and mitochondrial transcription factor A), antioxidant enzymes (copper-zinc superoxide dismutases and manganese superoxide dismutase), osteoblast differentiation molecules (bone morphogenic protein-2/7, osteoprotegerin and Runt-related transcription factor-2) and mineralization by upregulation of ALP and alizarin red staining, which were decreased by ZnPP and high glucose oxidative stress. Similarly, gomisin A inhibited ROS which was increased by ZnPP and the high glucose-mediated oxidative stress.</P> <P><B>Conclusions</B></P> <P>The findings demonstrated the antioxidative effects of gomisin A, and its role in mitochondrial biogenesis and osteoblast differentiation. It potentially regulated osteoblast differentiation under high glucose-induced oxidative stress <I>via</I> upregulation of HO-1 and maintenance of mitochondrial homeostasis. Thus, gomisin A may represent a potential therapeutic agent for prevention of bone fragility fractures and implant failure triggered by diabetes.</P> <P><B>Graphical abstarct</B></P> <P>[DISPLAY OMISSION]</P>

데이터 마이닝을 이용한 산업재해 예측모델에 관한 연구

이관형,정호근,박정선 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.4

목적 : 우리나라 전체 산업재해의 발생패턴과 추이를 파악하구 미래시점에 발생할 수 있는 산업재해자수를 예측 개발하여 장단기 산업보건 예방정책을 수립하는데 기여하고자 한다. 방법 : 예측모형에 사용된 자료는 1986년 1월부터 1999년 7월 까지 발생된 월별 누적 재해자수이며, 이 자료로부터 테이터 마이닝 기법을 사용하여 미래시점의 산업재해자 예측모델을 개발하였다. 결과 : 163개월 분의 산업재해 발생자료로부터 미래시점의 산업재해자수를 예측한 결과, Robust한 예측모형은 Winter∼method multiplicative in exponential smoothing로 예측력이 95%을 보였다. 산업재해 시도표를 탐색하면 전체적으로 산업재해자는 감소추세를 보이며, 순환주기를 1년으로 보면 2월과 9월이 가장 낮고, 6, 7, 10, 11월에 재해가 가장 많이 발생됨을 알 수 있었다. 월 평균 재해자 발생규모는 8,709명이다(95% CI;8277명, 9140명), 개발된 예측모형으로부터 1999년 8월 이후의 산업재해 발생자 규모를 보면, 1999년 12월과 2000년 1/4분기에 급격히 감소추세를 보이다가 2/4분기 시점을 정점으로 다시 재해자수가 증가할 것으로 예측된다. 결론 : 개발된 윈터스 모형을 이용한 미래시점의 산업재해 월별 발생 예측치는 (Table 3)과 같다. 예측치를 보면 1999년 긴월에서 2000년 1월, 2월에 급격히 감소추세에서 2000년도 2/4분기에 다시 서서히 증가하고 있다. 그리고 과거시점과 미래시점의 월별 산업재해 발생 실측치와 예측치 시도표는 Fig. 12와 같다. 또한 1998년에는 전반적인 발생추이 경향이 무너졌는데, 이는 한국 경제의 크나 큰 사건인 1997년 10월에 발표한 IMF에 의한 산업전반의 침체가 개입된 것으로 판단되며, 1999년에는 경기침체에서 벗어나 경제가 활성화 국면이 된다면 10월, 11월에는 이전보다 다소 재해자가 증가할 것으로 예상된다. 그리고 시간이 지남에 따라 추가적으로 발생된 월별 산업재해자수를 개발된 모델에 투입시키면서 검증과 평가를 통해모델을 정립할 계획이다. Objectives : This study is to see the transition and pattern of the industrial i울ureal worker, and to develop the prediction model. Methods : The data of the study are based on the samples from data-warehouse of Occupational Safety & Health Research Institute and are summed monthly from Jan 1986 to Dec 1999. This study data used data mart and Meta data from DW in KOSHA. The prediction model of the injured worker in Industry is designed by using a winters time series method after data preparing (i. e. sample, explore, modify) from DW. Results : Thls predicted model obtained Winters-method multiplicative in exponential smoothing among applied all models, after the tlme series (total 163 months). It showed that the prediction power was 95.5 %. Conclusions : In the process of exploring the data, totally the rate of industrial injureal workers reduced, and in the yearly circulation, in February and September the number is the lowest but in June, July, October and November the higher. The number of monthly average injureal workers is 8709 (95 % confidence interval 8277, 9140). From the developed prediction model, since Aug 1999 the industrial injureal worker reduced rapidly in Dec 1999 and first period of 2000. But In second period of 2000 the number of the injured workers is increasing. To conclude, as the total economic situation is becoming better in 2000 than In 1999, its is supposed that the injured workers will increase more than the predictive injured workers because of the increase of production rate and labor force.

성공적인 기업자원계획 시스템 도입 방안

이항,서의호,이근수 한국경영과학회 1998 經營 科學 Vol.15 No.2

오늘날의 기업환경에서 정보시스템은 기업에 있어서의 경쟁우위를 확보해주는 아주 보편적이고 중요한 수단이 되었다. 정보기술이 발전해 감에 따라 기업에서의 정보 시스템은 이제 거의 전분야에 걸쳐 활용되고 있다. 독자적으로 확대 구축된 정보시스템 간에는 상호통합의 필요성이 증가 되었다. 최근에 등장한 기업자원계획 시스템은 전사 자원을 통합 관리하여 생산성과 효율성을 크게 향상시켜 줄 뿐 만 아니라, 패키지로 되어 있어 단기간에 구축이 가능한 획기적인 정보 시스템으로 많은 기업들의 주목을 받고 있다. 기업자원계획 시스템을 구축하려면 리엔지니어링이 필연적으로 따르게 된다. 많은 기업들이 정보 시스템의 구축 또는 그를 이용한 리엔지니어링을 단지 비용절감이라는 소극적인 목적을 위한 수단으로 인식한데서 문제가 있었다. 리엔지니어링은 단지 업무 프로세스를 재구축하는 것이 아니라, 기업의 문화자체를 바꾸게 되고 기존의 장점까지도 버려야 할지도 모른다. 기업문화는 프로세스라기 보다는 인적자원의 몫이다. 리엔지니어링에 따른 종업원들의 혼란과 저항은 정보시스템 구축을 어렵게 만들고, 직업문화에 악영향을 줄 수도 있다. 기업의 업무 전반을 지원해주는 통합된 시스템은 업무 대체를 위한 수단으로 보다는 기업의 경쟁력을 높여주는 전략적 목적으로의 사용이 중요하며, 경쟁우위의 지속성을 확보할 수 있어야 한다. 기업자원계획 시스템의 구축 그 자체에 관심을 가질 것이 아니라, 장기적인 관점에서 기업의 전략을 뒷받침해 줄 수 있는 sustainer로서의 역할을 염두에 두어야 한다. 정보 시스템이 경쟁우위를 지속시켜주는 역할을 할 수 있으려면 인적자원에 대한 관심이 무엇보다도 중요하다.

고정층과 순환유동층에서 CaSO_4의 환원반응에 대한 온도와 CO농도의 영향

배달희,류호정,박재현,이창근,선도원,이동규 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.1

순환유동층 석탄연소로에서 석회석에 의한 찰황반응에 영향을 미치는 CaSO_4의 환원반응에 의한 SO-2 재배출을 실험적으로 확인하고 SO_2의 재배출에 미치는 온도와 CO 농도의 영향에대해 상용 순환유동층에 적용할 수 있는 기초자료를 확보하기 위해 고정층과 순환유동층에서 층물질로 석회석과 상용 순롼 유동층 하부회를 이용하여 환원실험을 수행하였으며 CaSO_4의 환원반응에 미치는 온도와 CO의 영향을 측정 및 해석하였다. 고온조건에서 CO가 환원제로 작용하여 C_aSO_4로부터 SO_2가 재배출되는 현상을 확인하였으며 CaSO_4로부터 SO_2의 재배출은 온도와 CO농도가 증가함에 따라 증가하였다. 본 실험의 결과에 의해 온도가 증가함에 따라 석회석에 의한 탈황율이 감소하는 현상을 환원이론으로 설명할 수 있다는 것이 확인되었다. For qualitative understanding of the sulphur capture process in a circulating fluidized bed fumace, the effects of temperature and CO concentration on the reactivity of partially sulfated CaO were experimentally examined. The tests were performed in a fixed bed reactor and lab-scale circulating fluidized bed reactor. The materials used were partially sulfated domestic limestone and bottom ash drained from commercial circulating fluidized bed furnaces. The re-emission of SO_2 from partially sulfated limestone and bottom ash increases with temperature as well as with concentration of the reducing agent CO. From the results of this study, the temperature dependence of sulfation could be explained by reduction theory.

Inducing apoptpsis by the inhibition of c-myb in oral squamous carcinoma cell line, KB cell

Lee,Jung-Chang 대한구강생물학회 2007 International Journal of Oral Biology Vol.32 No.4

Oral squamous cell carcinoma (OSCC) is the most commonmalignancy and is a major cause of worldwide cancer mortality.The proto-oncogene c-myb plays an important role in regulationof cell growth and differentiation, and it is expressed at highlevels in hematopoietic cells and many other types of cancers.However, the function of c-myb is not well known in OSCC.The present study aimed to reveal the function of c-myb and totest the alternation of cell growth and signaling by c-myb inOSCC. In this study, c-myb and dominant-negatibe myb(DN-myb) were expressed in an adenovirus-mediated gene deliverysystem to KB cells. The over-expressed c-myb broughtincreased cellular proliferation compared with control cells.However, DN-myb infected KB cells showed significantreduction of cell growth and enhanced induction of apoptosis toactivate PARP and caspase 9. c-myb induced increase of IGF-I,-II and IGF-IR expressions while DN-myb down-regulatedthese expression. Activation of ERK and Akt/PKB pathwaywas shown only in c-myb transduced cells. These findingssuggest that the role of c-myb in cell growth of oral cancer cellsis partially mediated through the modulation of IGFs, ERK andAkt/PKB. From this results, DN-myb is strongly recommendedas a curable gene for the treatment of c-myb dependentmalignancies such as OSCC.

LPS로 유도된 Human Dental Pulp Cells에서 Schisandrin C 의 항염증 효과

Jyoti Shrestha Takanche,Sang Won Lee,Yae Jin Kim,Young Hee Lee,Jeong Seok Kim,Ji Eun Kim,Ho Keun Yi,Sin Hee Han 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10

Background : Tooth vitality is reflected by the health of dental pulp. Schisandrin C is a natural compound extracted from the fruit of Schisandra chinensis which has anti-inflammatory and anti-oxidant properties. The role of Schisandrin C on human dental pulp cells (HDPCs) has not been studied yet. This study examined the properties of Schisandrin C as an anti-inflammatory and anti-oxidant compound, and whether its characteristics promote mitochondrial biogenesis in HDPCs. Methods and Results : HDPCs were extracted from fresh third molars and cultured. Reactive oxidative stress (ROS) and nitric oxide (NO) formation were analyzed by a Muse cell analyzer. Western blotting and gelatin zymography were used to identify the presence of anti-oxidants, as well as inflammatory and mitochondrial biogenesis. Confocal microscopy was used for the detection of mitochondrial activity. Schisandrin C inhibited lipopolysaccharide (LPS)-stimulated inflammatory molecules; intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), matrix metalloproteinase-2 and -9 (MMP-2/9), NO production, ROS formation and the mitogen-activated protein (MAPK) pathway through minimizing the nuclear factor kappa B (NF-kB) translocation. Schisandrin C increased the expression of superoxide dismutase (SOD) enzymes as well as heme oxygenase-1 (HO-1) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a) through the phosphorylated-protein kinase B (p-AKT) and nuclear factor erythroid 2-related factor-2 (Nrf-2) pathways. The anti-inflammatory and anti-oxidant properties of Schisandrin C promoted mitochondrial biogenesis. Conclusions : These results suggest that Schisandrin C may be used as an anti-inflammatory compound to reduce oral inflammation such as pulpitis.

Some Properties of Gluco - Amylase Produced by Pediococcus halophilus

Ho Keun Yi,Hae Ki Min,Yong hyo Jang,Kook Hee Kang 한국미생물생명공학회(구 한국산업미생물학회) 1991 한국미생물생명공학회 학술발표초록집 Vol.1991 No.1

The current status and outcomes of in-hospital P2Y12 receptor inhibitor switching in Korean patients with acute myocardial infarction

( Keun-ho Park ),( Myung Ho Jeong ),( Hyun Kuk Kim ),( Young-jae Ki ),( Sung Soo Kim ),( Youngkeun Ahn ),( Hyun Yi Kook ),( Hyo-soo Kim ),( Hyeon Cheol Gwon ),( Ki Bae Seung ),( Seung Woon Rha ),( Shu 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.2

Background/Aims: While switching strategies of P2Y12 receptor inhibitors (RIs) have sometimes been used in acute myocardial infarction (AMI) patients, the current status of in-hospital P2Y12RI switching remains unknown. Methods: Overall, 8,476 AMI patients who underwent successful revascularization from Korea Acute Myocardial Infarction Registry-National Institute of Health (KAMIR-NIH) were divided according to in-hospital P2Y12RI strategies, and net adverse cardiovascular events (NACEs), defined as a composite of cardiac death, non-fatal myocardial infarction (MI), stroke, or thrombolysis in myocardial infarction (TIMI) major bleeding during hospitalization were compared. Results: Patients with in-hospital P2Y12RI switching accounted for 16.5%, of which 867 patients were switched from clopidogrel to potent P2Y12RI (C-P) and 532 patients from potent P2Y12RI to clopidogrel (P-C). There were no differences in NACEs among the unchanged clopidogrel, the unchanged potent P2Y12RIs, and the P2Y12RI switching groups. However, compared to the unchanged clopidogrel group, the C-P group had a higher incidence of non-fatal MI, and the P-C group had a higher incidence of TIMI major bleeding. In clinical events of in-hospital P2Y12RI switching, 90.9% of non-fatal MI occurred during pre-switching clopidogrel administration, 60.7% of TIMI major bleeding was related to pre-switching P2Y12RIs, and 71.4% of TIMI major bleeding was related to potent P2Y12RIs. Only 21.6% of the P2Y12RI switching group switched to P2Y12RIs after a loading dose (LD); however, there were no differences in clinical events between patients with and without LD. Conclusions: In-hospital P2Y12RI switching occurred occasionally, but had relatively similar clinical outcomes compared to unchanged P2Y12RIs in Korean AMI patients. Non-fatal MI and bleeding appeared to be mainly related to pre-switching P2Y12RIs.