Effects of Phenytoin and Diazepam on the Seizure Activity in the Cortical Dysplasia Animal Models

Kim, Si-Hyung,Choi, In-Sun,Cho, Jin-Hwa,Park, Eun-Ju,Jang, Il-Sung,Choi, Byung-Ju,Kim, Hyun-Jung,Kim, Young-Jin,Nam, Soon-Hyeun The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Dysplasia-associated seizure disorders are markedly resistant to pharmacological intervention. Relatively little research has been conducted studying the effects of antiepileptic drugs(AEDs) on seizure activity in a rat model of dysplasia. We have used rats exposed to methylazoxymethanol acetate(MAM) in utero, an animal model featuring nodular heterotopia, to investigate the effects of AEDs in the dysplastic brain. Pilocarpine was used to induce acute seizure in MAM-exposed and age-matched vehicle-injucted control animals. Field potential recordings were used to monitor amplitude and numbers population spikes, and paired pulse inhibition in response to stimulation of commissural pathway. Two commonly used AEDs were tested: diazepam 5, 2.5mg/kg;phenytoin 40, 60mg/kg. Diazepam(DZP) and phenytoin(PHT) reduced the amplitude of population spike in control and MAM-exposed rats. However, the amplitude of population spike was nearly eliminated in control rats as compared to the MAM-exposed rats. Pharmaco-resistance was tested by measuring seizure latencies in awake rats after pilocarpine administration(320mg/kg, i.p.) with and without pretreatment with AEDs. Pre-treatment with PHT 60 mg prolonged seizure latency in control rats, but not in MAM-exposed animals. The main findings of this study are that acute seizures initiated in MAM-exposed rats are relatively resistant to standard AEDs assessed in vivo. These data suggest that animal model with cortical dysplasia can be used to screen the effects of potential AEDs.

한국인 알코올의존환자에서의 제1형 부신피질자극 호르몬 분비 촉진 호르몬수용체의 유전자 다형성에 관한 연구

김철민,김성곤,김지훈,김현경,김미경,유정현,민은정 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

스트레스 반응에 중요한 역할을 하고 있는 CRHR1 유전자 SNP와 알코올 의존과의 연관성에 관한 선행 연구들이 있다. 이에 본 연구는 백인을 대상으로 조사했던 기존연구와는 달리 한국인 남녀를 대상으로 하여 알코올 의존군과 정상 대조군의 CRHR1 유전자형 빈도 및 대립유전자형 빈도를 비교하였다. 본 연구의 연구대상자들은 알코올 의존군 268명(남자 218명, 여자 50명)과 정상 대조군 137명(남자 78명, 여자 59명)이었다. CRHR1 유전자 SNP를 SNP database와 선행연구를 근거로 선정한 후, 연구 대상자들의 이러한 SNP 유전자형을 PCRRFLP방법으로 조사하였다. 그 결과, 한국인의 CRHR1 유전자 SNPs로 7개(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887)가 분석되었다. 이중 rs28364027의 A 대립 유전자의 빈도는 남녀 각각에서 알코올 의존군이 대조군보다 유의하게 높았다. 그리고 rs28364027의 AA 유전자형 및 A 대립 유전자와 rs878886의 CC유전자형 및 C 대립유전자의 빈도는 알코올 의존군내에서 여성이 남성보다 유의하게 높았다. 이러한 결과를 종합해 보면 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인의 중요한 요인일 가능성을 제시하고 있다. 또 이러한 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인에서 남녀간 차이를 설명할 수 있을 가능성도 시사하고 있다. Objectives:There were several preceding studies investigating the association between alcohol dependence and corticotropine releasing hormone receptor1(CRHR1) gene SNPs that play an important role in response to stress. The frequencies of CRHR1 genotypes and alleles were compared between alcohol-dependent patients and normal control subjects. Methods:The subjects were 268 alcohol-dependent patients(218 males, 50 females) and 137 normal controls(78 male, 59 female). CRHR1 gene SNPs were investigated according to the SNP database and the results from previous studies, and their genotypes were analyzed by polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP). Results:Seven CRHR1 gene SNPs(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887) were found in Korean subjects. The frequency of rs28364027 A allele was significantly higher in alcohol-dependent patients than normal controls in both genders. In alcohol-dependent patients, the frequencies of AA genotype and A allele of rs28364027, and of CC genotype and C allele of rs878886 were higher in female than male. Conclusion:These results suggest that CRHR1 gene SNP is one of the important genetic factors in the etiology of alcohol dependence. And also it is supposed that the different frequency of SNP genotype could explain stress-related gender difference in the genetic etiology of alcohol dependence.

24주간 추적하는 임상 연구에서 한국인 남자 알코올 의존 환자의 비재발률

강철중,김성곤,남궁기,조동환,이병욱,최인근,최용성,박성봉,제영묘,김현경,김성연 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.1

Objetives : In order to provide useful data tbr the further clinical studies with Korean alcohol-dependent patients, enrollment and non-relapse rates were investigated while alcohol-dependent patients wcre followed up for 24 weeks. Methods : The subjects of this study were alcohol-dependent male patients who, for the first time, visited a hospital among 3 groups of hospitals, University Hospital, General Hospital or Mental Hospital, or those who needed to be followed up after discharge from those hospitals. After assigning 12 subjects to each hospital, we investigated enrollment and non-relapse rates while cognitive-behavioral therapy and pharmacotherapy were provided. Results : 1) Forty-eight patients were enrolled and the rate of enrollment was 36.4%. The enrollment rate in the University Hospital group was 52.1%, in the General Hospital group 50.0%. and in the Mental Hospital group 10.4%. 2) In 48 patients, non-relapse rate was 27.1% at 12 week and 18.8% at 24 weeks after starting follow-up. It was 24.4% and 20.0% at 12 and 24 weeks, repectively, in the University Hospital group while the General Hospital group showed 33.3% and 16.7%, respectively, and the Mental Hospital Group showed 20.0% at both 12 and 24 weeks. Conclusion : These results provide important data for further clinical studies of Korean alcohol-dependent patients.

Priming 처리종자의 저장기간에 따른 발아력 전당함량 및 α-amylase 활력

김재현,이석순,박성태,정명근 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.3

벼 종자의 priming처리 후 저장성을 검토하기 위하여 저장기간과 저장온도에 따라서 종자의 발아력과 저장양분 및 발아에 관여하는 주요 효소활력의 변화를 조사한 결과를 요약하면 다음과 같다. 1. Priming한 종자를 6개월간 저장하여도 어느 저장온도에서나 발아율의 차이가 없었다. 2. Priming한 종자에서 발아속도(T_50)가 2∼3일 빨랐으며 3개월 동안은 변화가 없었고 3개월 이후 발아속도가 약간 감소하였으나 priming 처리하지 않은 종자에 비해서 빨랐다. 3. 발아균일도는 priming 처리효과가 6개월간 계속 유지되었으며 25℃보다 저온인 17℃에서 효과가 더 크게 나타났다. 4. α-amylase 활력은 저장기간이 길수록 높았고 전당함량의 경우 3개월 이상 저장하였을때 감소하였으나 발아력에는 영향을 미치지 않았다. 5. 종자 수확후 6개월 동안은 어느 시기에 priming 처리하여 저장하여도 발아력 및 종자의 활력은 유지되었다. This experiment was carried out to determine changes of seed germination ability, total sugar, and α-amylase activity to different storage period, and temperature after seed priming treatment. In the storage during the six months, final germination rate and speed were similar to the untreated control seeds and the uniformity was continuously preserved. Germination speed(T_50) was faster than the control seeds by 2∼3 days. T_50 was a little decreased to priming effect after three months but faster than the control seeds. In the case of germination temperature 25℃, germination uniformity was decreased to long term storage periods. Total sugar content was increased to compare with the control seed during the one months at -10, 5 and 20℃ and total sugar content was decreased after two months. α-amylase activity was increased to storage of long term period and seed of storage temperature 5℃ was increased compared with -10 and 20℃. Germination ability and α-amylase activity was preserved continuously during six months after priming treatment.

EDIFACT에서 따른 EDI 변환처리 시스템 설계구조

김선형,남성현 순천향대학교 1994 논문집 Vol.17 No.1

EDI intended to actualize paperless eleltronic documents trade among enterprises or trade partners. It is forecasted broad usage as a way of strategy for survival of enterprises and making sure of competitive power. X.435. extended MHS(CCITT recommendation X.400 '88 version), is applied to communication protocol for EDI. EDI translation system that imported from overseas is operated by several enterprises and organizations in domestic, but not existence to develope independently and its area highly depend on external product. Hence, this paper suggests the sturcture of EDI translation system which is one of the core of EDI as basic study for development in domestic, and suggests the implementation method emphasizing flat-file creative aspect which is the core of EDI translation system. Also, for verification to validation of suggested sturcture and implementation method, it is proved through simple modeling. We show that the advantages of flat-file creation are that EDI translation system is easily structurized, modulized and extended in system later.

상치와 부추의 구조탄수화물의 정량분석 방법간의 비교연구

홍상식,윤수현,김경자,김대진 東亞大學校 附設 生活科學硏究所 1998 생활과학연구 논문집 Vol.6 No.-

This study was conducted to determine several analytical methods to quantitative structural carbohydrate(dietary fiber). Two kinds of vegetables(lettuce and leek) with each, having several components-dietary fiber(DF, includ nondigestible component), total dietary fiber(TDF), neutral detergent fiber(NDF), protein corrected NDF(c-NDF), indigestible dietary fiber(IDF) and acid detergent fiber(ADF) were compared in this study. Two kinds of vegetable components were calculated to the regression equation and correlation coefficients between them-Y=39.955+0.37X(r=0.27) between DF(Y) and NDF(X), Y=53.346+0.12(r=0.08) between DF(Y) and c-NDF(X), Y=30.519+0.80X((r=0.81, p<0.05) between DF(Y) and ADF(X), Y=65.097-0.128X(r=0.26, p<0.01) between TDF(Y) and IDF(X), and Y=47.89+0.2X(r=0.49, p<0.05), between DF(Y) and TDF(X), respectively.

동등백분위법을 이용한 순발력 검사 방법간의 동등화 방안

남덕현,윤상화,김도준,박재우 龍仁大學校 體育科學硏究所 1999 體育科學硏究論叢 Vol.9 No.1

This paper is designed to equalize the points obtained from different tests of agility by means of equipercentile equating in accordance with grade and sex. The subjects consist of 923 high school students who have ever gone through the tests of power. As a result, I have reached the following conclusion. 1. I used equipercentile equating for recording the tests of power of the first male graders, coming up with equalized values such as 223㎝ in a standing board jump, 33.7m in five seconds' running and 42m in throwing, and 53m in a vertical jump at the 50 percent level. 2. The second male graders, coming up with equalized values such as 232㎝ in a standing board jump, 33.5m in five seconds' running and 42m in throwing, and 57㎝ in a vertical jump at the 50 percent level. 3. The third male grades, coming up with equalized values such as 242㎝ in a standing board jump, 34.8m in five seconds' running and 45m in throwing, and 57㎝ in a vertical jump at the 50 percent level. 4. The first female graders, coming up with equalized values such as 168㎝ in a standing board jump, 25.8m in five seconds' running and 10m in throwing, and 34㎝ in a vertical jump at the 50 percent level. 5. The second female grader, coming up with equalized values such as 174㎝ in a standing board jump, 25.8m in five seconds' running and 17m in throwing, and 31㎝ in a vertical jump at the 50 percent level. 6. The third male graders, coming up with equalized values such as 187㎝ in a standing board jump, 27m in five seconds' running and 16.6m in throwing, and 30㎝ in a vertical jump at the 50 percent level.

韓國産 天南星科 植物에 關한 本草學的 硏究

황상욱,김태현,宋昊埈,辛民敎 한국전통의학연구소 1999 한국전통의학지 Vol.9 No.1

For the purpose of developing Korean Herbalogy with the plants of Araceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been thoroughly investigated and the results obtained were as follows: 1. There were totaled to 14 genera and 42 species in Korea and among them medicinal plants are 7 genera, 23 species, some 55% in total but, the number of species may be added because of the similiar plants. 2. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Araceae family were classified as Rhizoma 20, Radix 10, Folium 4, Flos 3, Herba 1. Thus it was noticed that Rhizoma and Radix was the main kind. 3. According to sum of 42 species in Araceae family, they were classified into Arisaema genera 17, Acorus and Zantedeschia genera 4 each, Philodendron and Symplocarpus genera 3 each, Anthurium and Colocasia genera 2 each. Thus it was noticed that Arisaema genera was the main kind, some 40.5% in total. 4. According to nature and flavour of medicinal plants, they were classified into warmth and heat ; 25, cold and cool ; 3, balance 2 and unidentified 6. Thus it was noticed that warmth and heat is the main in nature and flavour of medicinal plants. 5. Comparing to whole medicinal plants 23 kinds, toxic drugs include minor toxin were 18 kinds, 78.3% of the whole. From this result, It was revealed that the plants for medical purpose in Araceae was 55% of the whole, in which RHIZOMA and RADIX was mostly abundunt. It is considered that many experiements and clinical approaches must be continued to use Araceae plants widely.

경기북부 접경지역의 개발에 대한 기본구상

조세현,김정태,오상근 忠南大學校 地域開發硏究所 1997 地域開發論叢 Vol.9 No.-

The main purpose of this study is to suggest the primary way of planning in north Kyonggy adjacent to DMZ. For this study, the current situations of north Kyonggy were surveyed and analyzed in terms of land use, economic-industry structure, SOC development etc. The results are as followed. ① The aspects of environment conservation and land use should be considered at the level of development strategies. ② The development of growth poles and the infrastructure should considered in accordance with the national strategy of unification.

소음 노출 남성 근로자의 고음영역 청력손실과 관련 요인

정상재,우극현,박완섭,유재영,최태성,김상우,김진석 대한산업의학회 2000 대한직업환경의학회지 Vol.12 No.2

Objectives : The purpose of the study was to assess the risk factors assumed to be related to the high frequency hearing loss in the noise-exposed male workers. Methods : 712 occupationally noise-exposed male workers were included in this cross sectional study. The subjects filled out a questionnaire on the history of noise exposure, hearing protection, otologic diseases, tobacco smoking, and were tested on height, weight, hematocrit, serum total cholesterol level, fasting plasma glucose level and arterial blood pressure level. Pure tone audiometry and otoscopy were conducted and the subjects were allocated into two groups according to whether they had 4000 Hz hearing loss or not. Results : The multiple logistic regression analysis showed that military noise exposure, poor wearing status of the hearing protection devices, increased age, long noise exposure duration, mean arterial pressure less than 80mmHg, high fasting plasma sugar are the factors significantly related to high frequency hearing loss (P〈0.05). On the contrary, there was no significant relationship between noise intensity, body mass index, serum total cholesterol level, smelling and high frequency hearing loss. Low hematocrit was shown to be a significant factor with a p-value less than 0.1. Conclusions : High frequency hearing loss in the noise-exposed male workers is related to military noise exposure, use of hearing protection devices, age, noise exposure length, fasting glucose level and mean arterial pressure, in this order. Appropriate use of the hearing protection devices has a great impact on the prevention of the noise-induced hearing loss.