Comparison of trophic factors changes in the hippocampal CA1 region between the young and adult gerbil induced by transient cerebral ischemia.

Yan, Bing Chun,Park, Joon Ha,Kim, Sung Koo,Choi, Jung Hoon,Lee, Choong Hyun,Yoo, Ki-Yeon,Kwon, Young-Geun,Kim, Young-Myeong,Kim, Jong-Dai,Won, Moo-Ho Kluwer Academic/Plenum Publishers 2012 Cellular and molecular neurobiology Vol.32 No.8

<P>In the present study, we investigated neuronal death/damage in the gerbil hippocampal CA1 region (CA1) and compared changes in some trophic factors, such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), in the CA1 between the adult and young gerbils after 5 min of transient cerebral ischemia. Most of pyramidal neurons (89%) were damaged 4 days after ischemia-reperfusion (I-R) in the adult; however, in the young, about 59% of pyramidal neurons were damaged 7 days after I-R. The immunoreactivity and levels of BDNF and VEGF, not GDNF, in the CA1 of the normal young were lower than those in the normal adult. Four days after I-R in the adult group, the immunoreactivity and levels of BDNF and VEGF were distinctively decreased, and the immunoreactivity and level of GDNF were increased. However, in the young group, all of their immunoreactivities and levels were much higher than those in the normal young group. From 7 days after I-R, all the immunoreactivities and levels were apparently decreased compared to those of the normal adult and young. In brief, we confirmed our recent finding: more delayed and less neuronal death occurred in the young following I-R, and we newly found that the immunoreactivities of trophic factors, such as BDNF, GDNF, and VEGF, in the stratum pyramidale of the CA1 in the young gerbil were much higher than those in the adult gerbil 4 days after transient cerebral ischemia.</P>

분석심리학적 관점에서 본 동의보감의 정신치료

박선영 ( Park Sun Young ),정인모 ( Jung In Mo ),여한구 ( Yeo Han Koo ) 한국정신분석심리상담학회 2022 정신분석심리상담(구 정신역동치료) Vol.8 No.-

본 연구는 Jung의 분석심리학적 관점에서 동의보감의 병리와 치료원리를 탐구한 연구이다. 연구 결과, 분석심리학의 핵심 개념인 대극의 원리가 동의보감을 관통하는 기본적인 철학임을 알 수 있고, 동의보감에 나타난 정신치료 원리는 3가지로 구분할 수 있다. 첫째, 정신의 작용은 에너지 균형과 순환을 나타내는 소장평형(消長平衡)의 원리로 모든 우주 만물이 형태나 성격이 바뀔 뿐 에너지는 사라지지 않고 균형을 이루는, 대극의 초월과 합일을 통해 계속 순환하는 만물의 생성과 소멸의 원리이다. 둘째, 정신병리는 대극의 불균형을 극복하려는 물극필반(物極必反)의 원리로 에너지가 한쪽으로 치우쳐 불균형이 심화되면 다시 반대쪽으로 에너지가 기울게 된다는 상호작용의 원리이다. 정신병리는 불균형을 만드는 에너지의 일방성과 에너지의 상호작용의 부재 및 부조화, 들고나는 에너지의 속도 차이에 의해 초래된다. 셋째, 정신치료는 불균형에서 조화와 균형을 회복하는 것이며 치우침 없이 균형을 유지하는 중화(中和)의 원리로 설명한다. 중화는 신체뿐만 아니라 정신의 에너지 불균형을 다루는 정신치료 원리이며, 이는 마음을 다스려 양생(養生)을 돕는 음평양비(陰平陽秘)와 대극의 균형과 합일을 통해 치료적 작용을 돕는 치이권형(治以權衡)으로 구분할 수 있다. 요컨대 동의보감은 정기신(精氣神) 대극(對極)의 상호관계 속에서 신체와 정신이 조화와 균형을 이루고 생리와 병리 및 치료의 원리를 다루고 있으며, 그 치료원리는 분석심리학적 관점의 대극의 합일과 개성화 과정으로 이해할 수 있다. 이를 통해 한의학의 치료에서 분석심리학을 기초로 한 개입 가능성을 살펴볼 수 있었다. The present study reviews the pathology and treatment principles of Donguibogam from the perspective of Jung’s analysis psychology in order to explore psychotherapy principles inherent in Donguibogam. The results indicate that the principle of opposing poles, a core concept of analytical psychology, is in line with Donguibogam. More specifically, three principles of psychotherapy are derived from Donguibogam. First, Sojangpyeonghyeong, a concept of the balance and circulation of energy, describes psychological functioning. Sojangpyeonghyeong, in which all things in the universe change only in forms or characteristics whereas their energy does not disappear but maintains its balance, is a principle of generation and extinction of things that circulate consistently through the unity and transcendence of two opposing poles. Second, Mulgeukpilban, a concept of overcoming the imbalance of opposing poles, accounts for psychopathology. It is a principle of interactions that, if energy is skewed to one side and its imbalance intensifies, the energy is tilted back to the other side. Psychological disorders develop if unilateral tendencies of energy create imbalance and occur from the absence and incongruity of interactions due to the confinement of energy and speed discrepancy in the ebb and flow of energy. Third, psychotherapy restores harmony and balance from imbalance, and is explained through JoongHwa, a concept of maintaining balance without bias. JoongHwa is a principle dealing with psychological as well as physical imbalances. It is categorized into two parts. One is Yinpyeongyangbi that helps protection from psychological disfunction. The other is Chiygwonhyeong, which helps therapeutic action through balance and unity of opposing poles. In summary, Donguibogam deals with the principles of physiology, pathology and treatment where the body and mind are harmonized and balanced in the mutual relationship of the Jung (精), Qi (氣) and Shin (神) polarities. The treatment principle could be interpreted as the unity and individualization of opposing poles from the perspective of analytical psychology. The findings implicate the plausibility of the psychotherapeutic application based on analytical psychology to the interventions of Korean medicine.

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

만선신부전 환자에서 관상동맥조영술 소견에 관한 연구

한대희,김성구,박상호,김성한,조원영,방덕원,조윤행,정의룡,은영근,권영구 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Patients with chronic renal fialure have a substantially elevated risk of death from cardiovascular diseases than do the general population. the patients with chronic renal failure are at significantly increased freqeuncy of hypertension, hyperlipidemia, and diabetes mellitus which are known to the risk factors of the coronary artery diseases, and the prevalence of the coronary artery diseases in chronic renal failure patients is highly associated with the hemodynamic disorder and metabolic abnormalities. therefore we expected that the coronary angiographic findings in patients with chronic renal failure should be different from the general population (control groups) and investigated the risk factors contributing to coronary artery diseases. Method : we have retrospectively compared the coronary angiographic findings of 44 patients with chronic renal failure on hemodialysis with that of 88 patients in the general population and investigated the factors contributing to the development and acceleration of coronary artery diseases in patients with chronic renal failure Result : Hypertension and diabetes mellitus which is risk factors for coronary artery disease is significantly increased in patients with chronic renal failure, in coronary angiographic finding the severity of the lesion is worse. the incidence of PCI or CABG of patients with chronic renal failure is more than that of control group but statistically no difference between patients with chronic renal failure and control group. the sex, the duration of disease, the duration of dialysis, serum creatinine in patients with chronic renal failure have no correlation to the prevalence of coronary artery disease and severity of lesion. hyperglycemic patients with chronic renal failure have high incidence of coronary artery disease and are worse in the severity of lesion Conclusions : There is significantly increased the pevalence and severity of involving multiple coronary artery diseses in hyperglycemic patients with CRF.

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

크론병에 대한 CoPPLX의 방어효과에 대한 연구

이호학,김유림,문창기,이재일,양병윤,김종수,이정민,정재혁,안석진,박상준,김윤권,김소연,김영중,조민구,최영자,장성조 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.1

급성 허혈성 뇌졸중에 대한 동맥내 혈전 용해술 후 발생한 혈복증 1예

정성구,김명천,고영관 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Although thrombolytic therapy is one of the most effective therapeutic option for acute ischemic stroke, hemorrhagic complication still remains major concern about its application. A patient with hemoperitoneum, who previously underwent thrombolytic therapy(intraarterial urokinase infusion, IAUK) for acute ischemic stroke was treated successfully with emergency transarterial embolization for rupture of hepatocellular carcinoma. We present a rare case of rupture of hepatocellular carcinoma after intraarterial urokinase infusion for acute ischemic stroke with brief review of the literature.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

병원전처치에 대한 의학적 지도관리 : 구급업무일지 분석을 통한 사후관리 Retrospective Run Record Review

정구영,김찬웅 대한응급의학회 1999 대한응급의학회지 Vol.10 No.4

Background : Emergency Medical Technicians can do emergency care only under the control of the physicians. Emergency medical services system(EMSS) in Korea is now extended from the level of basic life support(BLS) to advanced life support(ALS). Unfortunately we don't have any official medical control system yet. We and regional EMSS, 119 rescue team(BLS), have had monthly joint meeting to validate and improve the prehospital care since August, 1998. Methods : From August, 1998 to February, 1999, 1,708 patients were transported to Ewha Mokdong Hospital by regional 119 rescue team. Eight hundred and six(47.2%) run record were collected and analyzed. The appropriateness of the prehospital care were evaluated based on the comparison of assessment data and treatment data in each run record. The run reccord were divided into 5 groups; 1) necessary-adequate, 2) necessary-inadequate, 3) necessary-undo, 4) unnecessary-done, and 5) unnecessary-undo. 1) and 5) were judged as acceptable, and 2),3), and 4) as unacceptable. Results : Among 806 transported patients, 60.8% required one of more emergency care. 21.0% of required care were not provided, and 20.7% of provided care were not adequate. 78.8% of unprovided and 75.9% of inadequate care were 'airway and oxygen supply'. Overall unacceptable rate was decreased from 31.7% to 17.0% during first 5 months, but it rose up again to 24.7% after 2 months. Cardiopulmonary resuscitation(CPR) was performed in 29 prehospital cardiac arrest victims and admitted to ICU in 5 cases. CPR was not provided jin 8 necessary situations, and unnecessary CPR was done in 3 cases. Conclusion : EMSS in Korea also need a kind of medical control system, even for BLS level. Major portion of the quality assurance program could be 'airway and oxygen supply', but attention should be focused in cardiac arrest victims and CPR. Record keeping and reliance of run record data are now pending problems. Fire department should develop a formal medical control system and the referring hospital should have an organization for maintaining the quality of prehospital care.

제조업 종사 지체장애근로자의 스트레스 관련요인

정민예,박정일,구정완,노영만 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.1

목적: 본 연구는 지체장애근로자의 스트레스의 정도를 파악하고 이에 영향을 미칠 것으로 추정되는 여러 요인 들 즉 일반적 특성, 장애관련 특성, 직업관련 특성, 직무 스트레스 원, 중재변인들과의 관련성을 규명함으로써 지체장애근로자들의 스트레스의 예방적 관리 방안에 대한 방향을 설정하는데 필요한 자료를 구하고자 수행하였다. 방법: 본 연구의 대상자는 경·인 지방 제조업 종사 지체장애근로자로 방문과 우편 설문 조사를 통하여 응답한 341명이었다. 스트레스 수준의 측정은 신뢰도와 타당도가 인정된 Goldberg의 GHQ-60을 수정보완한 사회 심리적 건강 측정도구인 Psychosocial Well-being Index(PWI)를 사용하였다. 관련변수들은 일반적 특성, 장애관련특성, 직업관련특성과 직무 스트레스 원으로 하였고 기타변수로 조직 외 요인(가정요인, 사회적 자원), 개인관련요인(욕구 및 가치, A형 행동, 통제소재 및 인구통계 특성), 자아존중감, 대처전략으로 설정하였다. 수집된 자료는 SAS 6.12 통계프로그램으로 분석하였다. 결과: 지체장애근로자의 스트레스수준에 영향을 미치는 관련요인은 다변량 로지스틱 회귀분석을 이용하여 분석한 결과 본인이 지각한 건강상태가 좋지 않은 경우, 자아 존중감이 낮은 경우, 규칙적 운동을 하지 않은 경우, 업무 중 장애로 인한 어려움을 느끼는 경우, 집안이동능력이 독립적이지 못한 경우, 직업에서 집단 갈등이 있는 경우, 그리고 역할 갈등을 느끼는 경우, A형 행동 유형인 경우였다. 결론: 지체장애근로자의 스트레스 수준을 낮추기 위해서는 건강증진프로그램의 개발과 제공, 작업보조시설 지원과 더불어 집단갈등 및 역할갈등을 포함한 직업관련갈등을 감소시키기 위한 방안마련이 요구된다. Objectives: This study was performed to measure the stress level of physically disabled workers, and to inestigate the relationship between the stress dimensions and stress inducting factors of physically disabled workers in the Korean manufacturing industry. Methods: The sample used in this study consisted of 341 physically disabled workers who had been working at companies in Seoul, Inchon, and Gyung-gi provinces. The Psycho-social Well-being Index (PWI), a modified version of Goldberg's 60-items of General Health Questionnaire (GHS), was used to measure the stress level. The following independent and moderate variables were desinged for the same purpose. The independent variables were general and disability characteristics, workplace-related characteristics, and job stressor of the respondents. The other variables included organization-related stressors (social and family support) and person related stressors (needs and values, type A behavior pattern, locus of control and demographics), self-esteem and coping strategy. The collected data were analyzed using SAS version 6.12 program. Results: Multiple logistic regression analysis showed that the significant variables on the stress level of physically disabled workers were poor self-perceived health status, low self-esteem, absence of regular exercise, physically disabled condition affecting work performance capability, dependent mobility at home, interpersonal conflict, role conflict and A type behavior pattern. Conclusions: The results of this study suggest that appropriate health program should be established and provided for reducing the stress of physically disabled workers in the manufacturing industry. In addition, various vocational programs for decreasing interpersonal and role conflicts should be developed.