肝吸蟲症 診斷에 있어서 ELISA법의 應用에 관한 硏究

梁正成,李駿商,林漢鍾 고려대학교 의과대학 1983 고려대 의대 잡지 Vol.20 No.1

Human clonorchiasis is one of the most common trematode diseases and of very inportant public health porblems in Korea. Many techniques have been deviced for immunological diagnosis of clonorchiasis. But, these methods have been recognized as low specificity and sensitivity. In the present study the enzyme-linded immunosorbent assay(ELISA) test was demonstreted in the diagnosis of human clononchiasis using clonorchis VBS(veronal buffer solution) skin test antigen and peroxidase conjugated anti-human IgG, and ortho-phenylenediamine as a substrate by microminor modifications. The results obtained are as follows : 1. In the preliminary study optimal dilutions of conjugate, stock antigen and serum were 1/5,000, 1/400 respectively. 2. The O.D.(optical density) values of the ELISA in the sera of clonorchiasis cases using stock antigen and VBS skin test antigen were 1.347±0.728 and 2.332±0.420 respectively, and the mean O.D. value in the sera of clonorchiasis cases using VBS skin test antigen was higher than that of stock antigen in sensitivity. 3. During the storage in a refrigerator, the mean O.D. value of the ELISA in 138 sera of clonorchiasis cases was 1.674±0.601 in the storage for 1 month and that in 244 sera of clonorchiasis casse was 1,494±0.884 from 6 months to 3 years. 4. In the distribution of the O.D. Values of the ELISA in the sera of clonorchiasis cases, 115 (83.3%) out of 138 sera in the storage for 1 month and 161 (66.0%) out of 244 sera in the storage from 6 months to 3 years showed higher O.D. value than 1.000(O.D. at 488nm) which is referedn to the positive serum. 5. The O.D. values of the ELISA in the sera were compared with the degree of infection by E.P.G. (eggs per gram of feces) counts in the stools of the clonorchiais cases and the regression equation was applied for the evaluation: y=0.004x+1.6637 (x : 1/100 of E.P.G; Y : O.D. values) There was no correlation between the O.D. values of the ELISA and the number of egg In the results, the application of the ELISA in the diagnosis of human clonorchiasis using VBS skin test antigen within 1 month period storage seemed to be higher than stock antigen with long period storage in sensitivity. In conclusion, the present the present study suggests that the ELISA test can be applied in the diagnosis of human clonorchiasis as applicable and reproducible test in comparison with other immunological tests, especially in the sere-epidemiological study.

Precision-TPA를 이용한 구치부 회전시 근심 및 원심삽입법에 따른 동적인 치아이동 양상에 관한 실험 연구

양성재,정성권,노준,전윤식 대한치과교정학회 1999 대한치과교정학회지 Vol.29 No.4

회전된 구치를 개선시키기 위해 사용하는 precision-TPA(transpalatal arch)를 삽입방법들-근심삽입법, 원심삽입법, 확장력을 부여한 근심삽입법 (이하 근심확장법) -에 따라 동적인 치아이동의 양상과 치열궁의 변화를 비교하기 위해 본교실에서 개발, 제작한 Calorific machine을 이용하여 실험한 후 회전된 치아와 고정치간의 이동양상을 계측하여 통계처리한 결과 다음과 같은 결론을 얻었다. 1. 구치간 폭경은 근심삽입법에 의해 감소하였고, 원심삽입법과 근심확장법에 의해서는 증가하였다. 2. 원심삽입법에 의해서 치열궁 장경이 가장 증가하였고, 근심확장법, 근심삽입법순으로 증가하였다. 3. 근심삽입법의 회전중심은 근심소와(mesial pit)와 중심소와(central pit)사이에위치하였고, 원심삽입법에서는 원심소와(distal pit)와 원심설측교두 (distolingual cusp)사이에, 근심확장법은 원심소와(distal pit)에 위치하였다. Dr. Youn-Sic Chun and coworkers invented the new machine for getting information about the relative effectiveness of the orthodontic appliances and we named it Calorific machine. The author and colleagues used this machine to compare with tooth moving mechanism by 3 types of the insertion method of precision-TPA for derotating the posterior teeth. We measured the distance of tooth movement and found out the rotational center on the occlusal X-ray film and the tooth movement on the occlusogram and then processed paired t-test by SAS program. The results were as follows : 1. In the intermolar width, the mesial insertion method showed the loss of distance, and the other methods (-distal insertion method, mesial expansion method-) showed the increase of distance. 2. In the arch length, the distal insertion method was exhibited as most recommandable way for increasing the arch length. 3. The rotation center of the mesial insertion method for derotating the molar, was located between mesial pit and central pit of the lower 2nd molar. And, in the distal insertion method, it was located between distal pit and distolingual cusp. and in the mesial expansion method was located at distal pit.

완전 기저 동맥 중복과 동반된 모야모야병(Moyamoya disease) 1예

권준범,양현덕,한범기,이지용,문준식,이성수 대한신경과학회 1999 대한신경과학회지 Vol.17 No.2

성숙한 Longissimus Dorsi근과 Soleus근의 근원섬유단백질의 특성비교

양융,최혁준,윤창훈,신완철,오상환 한국노화학회 1991 한국노화학회지 Vol.1 No.1

수근관 증후군에서의 무증상성 척골신경병증

문준식,이성수,이지용,한범기,권준범,양현덕,이성익 대한신경과학회 1999 대한신경과학회지 Vol.17 No.2

급성충소돌기염의 임상 : 병리학적 고찰

양기화,조준필,하영천,한진우,정학재,손수창,이성희,황재훈,신철호 최신의학사 1989 最新醫學 Vol.32 No.4

肝吸蟲 感染家兎에 있어서 感染强度, 經過 및 治療에 따른 ELISA抗體價의 變動에 관한 實驗的 硏究

梁元容,李駿商,林漢鍾 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.3

Human clonorchiasis is one of the most common trmatode diseases and of very important pubic health problems in Korea. In the present study, the changing patterns of specific IgG antibody was demonstrated by the enzyme-linked immunosorbent assay(ELISA) in the sera rabbits infected with clonorchis sinensis. The O.D> (optical density) values of the ELISA in the sera were merwured according to intensity and course of infections and tendency of decrease after chemotherapy. The ELISA was performed with veronal buffered saline antigen of clonorchis, peroxidase conjugated antirabbit IgG and ortho- phenylendiamine as a substrate by micro method. In the 3 groups of rabbits infected with 50(A group), 500(B group) or 100×5(C group) metacercariae, the sera were prepared biweekly up to 18th week before chemotherapy, and biweekly up to 24th week after chemotherapy. The results were as follows: 1. O.D. values increased gradually with lapse of time before treatment. The O.D. values indicated firstly 1.000 or more in 8 weeks after infection in A group and 4 weeks after infection in both B and C groups. The O.D. values reached plateau at 10 weeks after infection in A group and 8 weeks after infection in both B and C groups. Regression equations between the O.D. values of the ELISA and period(week) after infection among 2 groups were as fllows, y=0.09x+0.319 in A group y=0.08x+ 0.826 in B group and y=0.08x+0.807 in C group. 2. The O.D. values in A group which had been treated decreased gradually and became preinfection level at 24 weeks after chemotherapy. On the contrary the O.D. values in A group which has not been treated showde plateau. The O.D. values in both B and C groups which had been cured decreased gradually and became preinfection level at 24 weeks after chemotherapy.

실린다라이나의 磨滅特性에 미치는 붕소合金??鐵의 影響

朴常輔,崔洋鎭,朴相俊 大田開放大學 1986 論文集 Vol.4 No.-

파킨슨씨병 환자에 있어서 자가부신수질 이식술

정상섭,박상근,오성훈,김선호,윤도흠,박정수,양우익,최인준,안영수,김경환,박창일,김진수,이현철 대한신경외과학회 1988 Journal of Korean neurosurgical society Vol.17 No.6

Recent experimental studies and clinical cases have suggested that grafting tissue from the adrenal medulla into the brain may alleviate the symptoms of Parkinson's disease. Autologous adrenal medullary grafts into the striatum was performed stereotactically in two patients with Parkinson's disease. Both patients were bedridden preoperatively, but 4 months after the surgery both became ambulatory and one was even capable of returning to his job. Even though the number of cases was small⑵ and the follow-up period was short, this study shows that adrenal medullary transplantation into the striatum may have some beneficial effects in patient with Parkinson's disease. Further experimental and clinical trials are necessary to see whether this procedure will be a recommendable surgical treatment for patients suffering from Parkinson's disease.

水稻의 登熟向上에 關한 硏究

諸商律,李寅杰,김랑춘,李準琸,張南日,洪鍾旭 慶北大學校 1974 論文集 Vol.18 No.-

This study was made to get the cultural information for increasing yield with recently improved variety, investigating the effects of different transplanting date and seed size, the number of plant per hill on the growth, and the yield and yield components in rice. The results obtained were summaried as follows: 1. In larger seed, the percentage of germination was higher and the early growth was more vigorous. However, there appeared no effects of seed size in the later growing period. 2. When the young plants sown on April 26 were transplanted on 5th, 15th, 25th of June and 5th of July with 10-days interval, the heading date was promoted in earlier transplanted plots. The days to heading, however, was shortend. 3. In the case of the earlier transplanting in Tongil variety, the maximum number of tillers was increased, the percentage of effective tillers was decreased, and the rate of ripened grains was slightly higher. The yield was the highest in the transplanted plot of June 25. The next came the transplanted plots of 25th, 5th of June and 5th of July. In the early transplanted plot of Palkweong variety, heading was also promoted. The days required for heading were also proportionly shortend, the number of the effective tillers was smaller and the percentage of ripened grains also decreased. The productivity was the highest in transplanted plot of June 25. 4. When the number of plants per hill was increased, the heading date was promoted, the number of panicles was high and the number of grains per panicles was decreased. In the early transplanting of Tongil variety, the yields was the highest in 4-plants plot. When transplanted late, it was the highest in 6-plant plot. In Palkweong variety, there was general tendency of increasing of yield as the number of plants per hill increased. 5. The dwarf disease of Tongil variety and the stripe disease of Palkweong variety occurred much in case of the lesser number of plants per hill and the earlier transplanting.