기업간의 전자상거래 - 전자상거래의 증대로, 기업은 대폭적인 비용절감과 신규판매의 확대 실현

오상환 대한기계설비건설협회 2005 기계설비 Vol.3 No.-

화재예방안전 목표향상을 위한 소방관련 제도개선

오상환,O, Sang-Hwan 한국방재안전학회 2015 防災安全 Vol.5 No.1

최근 들어 전국의 곳곳에서 화재가 발생하여 귀중한 인명과 재산을 잃는 대형 참사(慘事)로 이어지고 있다. 화재가 발생했을 때에 초기진화(初期鎭火) 및 인명(人命)의 대피시간(待避時間)인 소위 골든타임은 대략 5분정도이다. 또한 화재사고를 직접체험하게 되면 패닉(panic)현상을 초래하여 우왕좌왕, 혼란의 블랙홀(Black hole)에 함몰(陷沒)되게 마련이다. 의정부 아파트 화재에서처럼 대부분의 골목길은 많은 차량들이 주차되어 소방차 긴급출동에 장애가 되고 있는 실정이다. 보편적으로 화재발생 시 소방대는 5분 내에 출동을 목표로 훈련을 한다. 하지만 화재현장에서 소방대에 화재신고가 신속히 이루지지 못하는 지체시간(遲滯時間)을 감안하면 소방대가 현장에 도착하여 진압작업을 개시하는 시점에는 이미 골든타임을 벗어나 화재가 확산되어 있는 상황인 것이다. 그러므로 골든타임을 감안(勘案)하여 소방대의 출동이전에 자체적으로 초기진압을 할 수 있는 방재시스템을 구축(構築)하여야 하는 것이다. 이에 소방대의 도착이전의 골든타임 이내에 자체적으로 조기진압 및 피난을 할 수 있어야 할 것이며, 다만 출동한 소방대는 인접한 건물이나, 주유소 등으로의 연소 확산(延燒 擴散)을 방지하는 역할로 개념(槪念)을 정리할 필요가 있는 것이다. 소방관련 법령 및 화재안전기준 등은 수시로 개정 발전 되어 오늘날에 이르렀다, 하지만 아직까지도 불합리하고 모순된 규정 등이 곳곳에 산재해 있다. 이에 틀에 박힌 고정관념에서 벗어나 보다 논리적이고 합리적이며 현장여건에 부합하는 선진화된 소방제도를 구축하여 화재참사로부터 국민의 생명과 재산보호는 물론 그 손실을 최소화하기 위한 선진화된 소방관련 제도로 정착시켜야 할 것이다.

방호성능 보장을 위한 소방규제 발전방안

오상환 대한지방행정공제회 2018 도시문제 Vol.53 No.594

성공하는 시간관리와 인생관리를 위한 10가지 자연법칙

오상환,O, Sang-Hwan 한국경영기술컨설턴트협회 2004 컨설턴트 Vol.21 No.-

실리콘 인상재의 보관온도에 따른 특성변화

오상환,문승균,김경남 한국치위생과학회 2010 치위생과학회지 Vol.10 No.6

The purpose of this study was to evaluate the physical properties of hydrophilic polyvinyl siloxane impression materials as change of material's storage temperatures. Working time, strain-in-compression, elastic recovery and consistency were tested according to ISO Standard NO. 1563. The results are as followed. 1. Working time decreased in cold storage. 2. Strain-in-compression was different in storage temperatures. Material's strain-in compression in cold temperatures were higher than in room temperature and in incubator. 3. A coefficient of elastic recovery varied by storage temperatures. The rate in cold temperature was the lowest and in incubator was the highest. 4. Consistency of impression substance different in storage temperatures. The extent in cold temperature is the highest and in incubator was the lowest. Statistical analysis(SPSS 14.0k, p>0.05) showed that storage temperature affect to material's physical properties. We recognized that the physical properties of polyvinyl siloxane impression materials were changed according to storage temperature.

사회적 기업가정신이 시장지향성에 미치는 영향

오상환,윤대홍,옥정원 대한경영정보학회 2017 경영과 정보연구 Vol.36 No.5

The purpose of this study was to empirically verify the effect of social entrepreneurship on market orientation. total of 500 questionnaires were distributed to workers in social enterprise and preliminary social enterprise. 202 questionnaires were used for final validation of research model, The hypotheses set in this study were validated through SPSS18.0 and LISREL8.3 based on the research model. The results showed that all hypotheses were accepted, except for 5 hypotheses(Hypothesis 1-1, Hypothesis 1-2, Hypothesis 1-3, Hypothesis 1-6, Hypothesis 1-9). First, we examined the effect that empathy might have on market orientation in connection with social entrepreneurship. The results suggested that empathy did not have a statistically significant effect on customer-orientation, inter-department cooperation and coordination, and competitor orientation. Second, we examined the effect that innovativeness might have on market orientation in connection with social entrepreneurship. The results showed that innovativeness had a positive(+) effect on customer-orientation and inter-department cooperation and coordination but did not have a statistically significant effect on competitor-orientation. Third, we examined the effect that risk-taking might have on market orientation in connection with social entrepreneurship. The results implied that risk-taking had a positive(+) effect on customer-orientation and inter-department cooperation and coordination but did not have a statistically significant effect on competitor-orientation. Finally, the relationship among market orientation variables was like this: The inter-department cooperation and coordination had a positive(+) effect on both customer-orientation and competitor-orientation. The results of this study are expected to provide a useful basis for overall understanding about the effect of social entrepreneurship on market orientation and present important theoretical and practical implications. 본 연구의 목적인 사회적 기업가정신이 시장지향성에 미치는 영향 관계를 실증적으로 검증하기 위해, 사회적기업 및 예비사회적기업 종사자를 대상으로 500부의 설문지를 배포하였으며, 202부의 설문지가 사용되었다. 연구모형을 토대로 SPSS18.0과 LISREL8.3을 이용하여 가설을 검증한 결과 5개의 가설(가설 1-1, 가설 1-2, 가설 1-3, 가설 1-6, 가설 1-9 )을 제외하고 모든 가설이 채택되었다. 이에 대한 구체적인 결과는 다음과 같다. 첫 번째, 사회적 기업가정신 중 공감성이 시장지향성에 미치는 영향을 살펴보면 다음과 같다. 공감성은 고객지향성, 부서 간 협력 및 조정, 경쟁자 지향성 모두에 통계적으로 유의하지 않는 것으로 나타났다. 두 번째, 사회적 기업가정신 중 혁신성이 시장지향성에 미치는 영향을 살펴보면 다음과 같다. 혁신성은 고객지향성과 부서 간 협력 및 조정에 정(+)의 영향을 미치고 있지만, 경쟁자지향성에는 통계적으로 유의하지 않는 것으로 나타났다. 세 번째, 사회적 기업가정신 중 위험감수성이 시장지향성에 미치는 영향을 살펴보면 다음과 같다. 위험감수성은 고객지향성과 부서 간 협력 및 조정에 정(+)의 영향을 미치고 있지만, 경쟁자지향성에는 통계적으로 유의하지 않는 것으로 나타났다. 마지막으로 시장지향성 변수들 간 관계는 다음과 같다. 부서 간 협력 및 조정은 고객지향성과 경쟁자지향성 모두에 정(+)의 영향을 미치고 있는 것으로 나타났다. 본 연구의 결과는 사회적 기업가정신이 시장지향성에 미치는 영향대한 종합적인 이해와 함께 중요한 이론적, 실무적 시사점을 제공할 것으로 기대된다.

[특집 : 대형화재와 방재] 화재예방 안전적폐 청산을 위한 건축 · 소방관련 제도개선

오상환 한국방재학회 2018 한국방재학회지 Vol.18 No.2

유전성 비만 마우스 ( ob / ob ) 에서 Retrovirus 를 이용한 Leptin 유전자의 전입 및 발현

오상환,조무연,정인철,변용준 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4

Background: Leptin gene is known to be related to obesity in human and animals and complete genetic defect of the gene in ob/ob mouse has been identified. Therefore, ob/ob mouse is widely used as an animal model for the study of etiology and therapy of obesity. The main biological function of leptin was thought to involve in the regulation of food intake and weight gain, however, the regulatory mechanisms by which leptin functions in the weight reduction and lowering the blood glucose level are uncertain. In the present study, retroviral-mediated leptin gene transduction into ob/ob mouse was attempted for the correction of biochemical parameters of obesity. Methods: Leptin cDNA was inserted into pLXSN retroviral vector (pLXSN-lep) and recombinant leptin expressing retrovirus particles (3 X10 CFU/mL) were produced in ?2 ecotropic packaging cells and subsequent transfection into PA317 amphotropic packaging cells. The leptin expressing recombinant viruses (LER) were transduced into NIH3T3 mouse fibroblasts and nsertion of leptin cDNA into chromosomal DNA of PA317 and MH3T3 mouse fibroblasts was confirmed by Southern blot hybridizations. Leptin mRNA and its protein expressed in the cells were identified by Northern blot hybridization and Western blot immunodetection method, respectively. LER were injected I. P. into ob/ob mice, and body weight, food intake, serum leptin level and blood glucose level were measured. Results: Expression of leptin was identified in PA317 and NIH3T3 mouse fibroblasts transduced with LER. Leptin content in sera of mice transfused with LER was drastically increased after 1 week and decreased to the almost basal level at 3 weeks after the transfusion. The body weight as well as food intake of ob/ob mouse transduced by LER decreased for the first 3 weeks and slightly increased thereafter. The reduction of both body weight and food intake in ob/ob mice transduced with LER was observed with the concomitant increase of serum leptin level, indicating that retroviral-mediated transduction of leptin gene in ob/ob mouse in vivo produced a biologically active leptin protein and released it into blood circulation. Conclusion: A transient expression of leptin cDNA in ob/ob mice by a retroviral-mediated transduction was performed and further studies are required for long term expression of the gene in vivo (J Kor Soc Endocrinol 15:502-512, 2000).

한국산 집먼지진드기 주알레르겐의 분리 및 합성

오상환,홍천수,박중원,김경섭,박상욱 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.1

Background: Purified major allergens of house dust mite are essential for evaluation of the allergic mechanism in molecular basis and development of new modalities of immunemodulation. Objective: In this study, we aimed to purif group 1 and group 2 allergens from Dermatophagoides pteronyssinus (Dp). In addition, cDNAs corresponding to Der pI and II in Korean Dp were isolated and recombinant Der p1 and Der pII were synthesized. Materials and method: Der pI allergen was purified by ammonium sulfate precipitation, anionexchange column chromatography, and gel filtrat,ion chromatography. Der pII allergen was purified by anion exchange chromatography, gel filtration chromatography, and a preparative isoelectric focusing method. Results: Eight hundred ug of Der pI and 50㎍ of Der pII were obtained from 100 g of culture medium and 1 g of mite bodies, respectively. The purities of these allergens were confirmed by SDS PAGE and the strong reactivity to the patient sera was identified. In order to produce a recombinant allergens, poly(A) RNA from house dust mites were isolated and used for cDNA synthesis by RT PCR. The cDNA was inserted into prokaryotic expression vector and the vectors were transformed into E. coli. A little amount of recombinant Der pI protein was produced due to the low solubility, and 1.2 mg of recombinant Der pII was produced from 1 L of E. coli culture medium. The antigenicity of Der pI was relatively weak, however, Der pII showed a strong antigenicity. Amino acid sequence of the amplified cDNA deduced from DNA sequences of Der pII showed 6 different variants. The variation of amino acid sequences suggests the possibility of high incidence of mutation of Der pII protein. Conclusion: A simplified method for the purification of Der pI and Der pII was developed. Recombinant allergens will be useful for the diagnosis and treatment of allergy with lower costs.

N-ras Mutation in a Human Hepatoma Cell Line PLC/PRF/5

오상환 암연구센터 1994 암연구의최신지견 Vol.1994 No.-