OPT-VCG: A Novel Proposal for 3D SoC Test Optimization

Jingbo Shao,Yongqing Fu,Xiaoxiao Liu,Guohui Zhou 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2

As an emerging technology for on-chip interconnect scaling in vertical direction in semiconductor industry, through-silicon-via (TSV) demonstrates its advantages and has been adopted for 3D SoC implementation. Optimal test architecture and test scheduling are significant for stacked 3D SoC design. However existing design methods cannot achieve both optimal test time and individual rationality. In this paper, game theory based 3D SoC test architecture optimization and test scheduling method is proposed under constraints of the available number of TSVs for test time minimization and rational test band width allocation. VCG algorithm is brought to 3D SoC design. Three kinds of stacked SoCs are built using ITC’02 SoC test benchmarks, and experimental results on them show the advantages of the proposed method over prior work.

CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin

Jingbo Ma,Xiaolong Xu,Chunjin Fu,Peng Xia,Ming Tian,Liuhai Zheng,Kun Chen,Xiaolian Liu,Yilei Li,Le Yu,Qinchang Zhu,Yangyang Yu,Rongrong Fan,Haibo Jiang,Zhifen Li,Chuanbin Yang,Chengchao Xu,Ying Long,J 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: It is highly desirable to develop new therapeutic strategies for gastric cancer given the low survival rate despite improvement in the past decades. Cadherin 17 (CDH17) is a membrane protein highly expressed in cancers of digestive system. Nanobody represents a novel antibody format for cancer targeted imaging and drug delivery. Nanobody targeting CHD17 as an imaging probe and a delivery vehicle of toxin remains to be explored for its theragnostic potential in gastric cancer. Methods: Naïve nanobody phage library was screened against CDH17 Domain 1-3 and identified nanobodies were extensively characterized with various assays. Nanobodies labeled with imaging probe were tested in vitro and in vivo for gastric cancer detection. A CDH17 Nanobody fused with toxin PE38 was evaluated for gastric cancer inhibition in vitro and in vivo. Results: Two nanobodies (A1 and E8) against human CDH17 with high affinity and high specificity were successfully obtained. These nanobodies could specifically bind to CDH17 protein and CDH17-positive gastric cancer cells. E8 nanobody as a lead was extensively determined for tumor imaging and drug delivery. It could efficiently co-localize with CDH17-positive gastric cancer cells in zebrafish embryos and rapidly visualize the tumor mass in mice within 3 h when conjugated with imaging dyes. E8 nanobody fused with toxin PE38 showed excellent anti-tumor effect and remarkably improved the mice survival in cell-derived (CDX) and patient-derived xenograft (PDX) models. The immunotoxin also enhanced the anti-tumor effect of clinical drug 5-Fluorouracil. Conclusions: The study presents a novel imaging and drug delivery strategy by targeting CDH17. CDH17 nanobodybased immunotoxin is potentially a promising therapeutic modality for clinical translation against gastric cancer.

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

Liu, Di,Zhang, Ting,Chen, Zhifei,Wang, Ying,Ma, Shuang,Liu, Jiyun,Liu, Jingbo The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.2

Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by $40{\mu}M$ $H_2O_2$. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of $14.48{\pm}4.04{\mu}M\;TE\;per\;{\mu}g/mL$). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

ECLOGITES AND THEIR COUNTRY GNEISSES STUDIES ON INCLUSIONS IN THE ACCESSORY MINERALS OF COUNTRY GNEISSES

LIU JINGBO,WU YIN,GUO LIANJIE 대한지질학회 1997 대한지질학회 학술대회 Vol.1997 No.

Database Performance Optimization for SQL Server Based on Hierarchical Queuing Network Model

Jingbo Shao,Xiaoxiao Liu,Yingmei Li,Jingyu Liu 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.1

With the ever-increasing complexity and variety of database workload, database application system has been imposed on higher and higher performance requirements. Database system consists of software and hardware. And the factors that affect database performance are uncertain. In order to tackle the issue of database system for SQL server, this paper proposes hierarchical queuing network model for performance prediction, and a model is established for both software resources and hardware resources, the nested resources are linearized by hierarchical calling, thus finding out the main factors for system performance bottleneck, and system performance is adjusted and optimized accordingly. The performance tuning algorithm for SQL server database based on the hierarchical queuing network is presented in detail. And TPC-C benchmark is adopted for simulation. Experimental results show the proposed method achieves 16.8% performance increase on average, and TPS is improved by 40% compared to previous method.

CCN4 Regulates Vascular Smooth Muscle Cell Migration and Proliferation

Hao Liu,Zhengjun Liu,Wenpeng Dong,Zhiqi Lin,Jingbo Lu,Heng Wan,Zhongxin Zhou 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.2

The migration and proliferation of vascular smooth muscle cells (VSMCs) are essential elements during the development of atherosclerosis and restenosis. An increasing number of studies have reported that extracellular matrix (ECM) proteins, including the CCN protein family, play a significant role in VSMC migration and proliferation. CCN4 is a member of the CCN protein family, which controls cell development and survival in multiple systems of the body. Here, we sought to determine whether CCN4 is involved in VSMC migration and proliferation. We examined the effect of CCN4 using rat cultured VSMCs. In cultured VSMCs, CCN4 stimulated the adhesion and migration of VSMCs in a dose-dependent manner, and this effect was blocked by an antibody for integrin 51. CCN4 expression was en-hanced by the pro-inflammatory cytokine tumor necrosis factor  (TNF-). Furthermore, knockdown of CCN4 by siRNA significantly inhibited the VSMC proliferation. CCN4 also could up-regulate the expression level of marker proteins of the VSMCs phenotype. Taken together, these results suggest that CCN4 is involved in the migration and proliferation of VSMCs. Inhibition of CCN4 may provide a promising strategy for the prevention of restenosis after vascular interventions.

The beneficial effect of ginsenosides extracted by pulsed electric field against hydrogen peroxide-induced oxidative stress in HEK-293 cells

Di Liu,Ting Zhang,Zhifei Chen,Ying Wang,Shuang Ma,Jiyun Liu,Jingbo Liu 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.2

Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by 40mM H2O2. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of 14.48 4.04mM TE per mg/mL). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.

Geographical distribution patterns of Metaeuchromius in Asia and description of a new species from China (Lepidoptera, Crambidae)

Jingbo Yang,Wei Liu,Weichun Li 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.3

The geographical distribution patterns of Asian Metaeuchromius are analysed with MaxEnt and ArcGIS based on known localities and nineteen environmental variables. The results suggest that the most highly suitable area of the genus located in southeastern China. Precipitation of the warmest quarter (Bio18) and temperature seasonality (Bio4) are the most significant variables affecting geographical distribution Metaeuchromius. Furthermore, a new species from Jiangxi Province, southeast China is added to the genus, Metaeuchromius grandispinata Li, sp. nov. Its habitus, tympanic organs and genitalia are illustrated, and two maps showing geographical distribution patterns of Metaeuchromius in Asia are provided.

Chemical Composition and Antimigraine Activity of Essential Oil of Angelicae dahuricae Radix

Jingbo Sun,He Li,Jinghui Sun,Huimin Liu,JianGuang Chen,Chunmei Wang 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.8

The aim of this study was to explore the chemical composition and the effect of essential oil of Angelicae dahuricae radix on a nitroglycerin (NTG)-induced rat model of migraine. The CO2 supercritical fluid extraction method was optimized for the extraction of essential oil of A. dahuricae radix (EOAD) and its chemical composition was determined. The migraine model was established by subcutaneous injection of NTG (10 mg/kg) 1 h after the last administration of EOAD. The therapeutic effect of EOAD and its underlying mechanism were assessed by monitoring behavioral changes, levels of nitric oxide (NO) in serum and brain tissues, plasma levels of calcitonin gene-related peptide (CGRP) and endothelin (ET), and ET/NO ratio. The optimal conditions for CO2 supercritical fluid extraction of EOAD, as determined by orthogonal test [L9(34)], were as follows: 2 h extraction time, 20 MPa pressure, 40°C temperature, and 30 mesh. The yield of EOAD was 1.8%. On gas chromatography–mass spectrometry, 45 peaks were found in EOAD, and 22 compounds were identified and quantified. The main constituents of EOAD were 1-dodecanol (13.71%), elemene (7.54%), palmitic acid ethyl ester (7.32%), α-pinene (6.25%), and 1-pentadecanol (6.08%). Compared with rat migraine model controls, EOAD (35, 70, and 140 mg/kg) significantly reduced the number of head shaking, head scratching, and hind leg shooting events, decreased serum and brain NO levels, decreased plasma CGRP, and increased ET levels in rats. ET/NO ratio was elevated to 28.68 in the EOAD high-dose group. EOAD ameliorates NTG-induced migraine in rats likely by modulating the levels of vasoactive substances.

Coordinated Attitude Control for Synthetic Aperture Radar Satellites with Quantization and Communication Delay

Jingbo Fu,Ming Liu,Huayi Li,Xibin Cao 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.7

This paper studies the coordinated attitude control problem of synthetic aperture radar satellites andproposes two types of terminal sliding mode control schemes. The first control law is investigated based on theconventional non-singular terminal sliding mode approach and modified Rodrigues parameters with the consideration of signal quantization and communication delay. Based on the first control scheme, we develop the secondcontrol method with an integral-based event triggering condition to reduce the updating frequency of the controlcommand from controllers to actuators. Finally, numerical simulation examples based on a cluster of three satellitesare presented to show the effectiveness of the proposed two designed control methods.