OPT-VCG: A Novel Proposal for 3D SoC Test Optimization

Jingbo Shao,Yongqing Fu,Xiaoxiao Liu,Guohui Zhou 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2

As an emerging technology for on-chip interconnect scaling in vertical direction in semiconductor industry, through-silicon-via (TSV) demonstrates its advantages and has been adopted for 3D SoC implementation. Optimal test architecture and test scheduling are significant for stacked 3D SoC design. However existing design methods cannot achieve both optimal test time and individual rationality. In this paper, game theory based 3D SoC test architecture optimization and test scheduling method is proposed under constraints of the available number of TSVs for test time minimization and rational test band width allocation. VCG algorithm is brought to 3D SoC design. Three kinds of stacked SoCs are built using ITC’02 SoC test benchmarks, and experimental results on them show the advantages of the proposed method over prior work.

Coordinated Attitude Control for Synthetic Aperture Radar Satellites with Quantization and Communication Delay

Jingbo Fu,Ming Liu,Huayi Li,Xibin Cao 제어·로봇·시스템학회 2019 International Journal of Control, Automation, and Vol.17 No.7

This paper studies the coordinated attitude control problem of synthetic aperture radar satellites andproposes two types of terminal sliding mode control schemes. The first control law is investigated based on theconventional non-singular terminal sliding mode approach and modified Rodrigues parameters with the consideration of signal quantization and communication delay. Based on the first control scheme, we develop the secondcontrol method with an integral-based event triggering condition to reduce the updating frequency of the controlcommand from controllers to actuators. Finally, numerical simulation examples based on a cluster of three satellitesare presented to show the effectiveness of the proposed two designed control methods.

CDH17 nanobodies facilitate rapid imaging of gastric cancer and efficient delivery of immunotoxin

Jingbo Ma,Xiaolong Xu,Chunjin Fu,Peng Xia,Ming Tian,Liuhai Zheng,Kun Chen,Xiaolian Liu,Yilei Li,Le Yu,Qinchang Zhu,Yangyang Yu,Rongrong Fan,Haibo Jiang,Zhifen Li,Chuanbin Yang,Chengchao Xu,Ying Long,J 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: It is highly desirable to develop new therapeutic strategies for gastric cancer given the low survival rate despite improvement in the past decades. Cadherin 17 (CDH17) is a membrane protein highly expressed in cancers of digestive system. Nanobody represents a novel antibody format for cancer targeted imaging and drug delivery. Nanobody targeting CHD17 as an imaging probe and a delivery vehicle of toxin remains to be explored for its theragnostic potential in gastric cancer. Methods: Naïve nanobody phage library was screened against CDH17 Domain 1-3 and identified nanobodies were extensively characterized with various assays. Nanobodies labeled with imaging probe were tested in vitro and in vivo for gastric cancer detection. A CDH17 Nanobody fused with toxin PE38 was evaluated for gastric cancer inhibition in vitro and in vivo. Results: Two nanobodies (A1 and E8) against human CDH17 with high affinity and high specificity were successfully obtained. These nanobodies could specifically bind to CDH17 protein and CDH17-positive gastric cancer cells. E8 nanobody as a lead was extensively determined for tumor imaging and drug delivery. It could efficiently co-localize with CDH17-positive gastric cancer cells in zebrafish embryos and rapidly visualize the tumor mass in mice within 3 h when conjugated with imaging dyes. E8 nanobody fused with toxin PE38 showed excellent anti-tumor effect and remarkably improved the mice survival in cell-derived (CDX) and patient-derived xenograft (PDX) models. The immunotoxin also enhanced the anti-tumor effect of clinical drug 5-Fluorouracil. Conclusions: The study presents a novel imaging and drug delivery strategy by targeting CDH17. CDH17 nanobodybased immunotoxin is potentially a promising therapeutic modality for clinical translation against gastric cancer.

The 14-3-3 Gene Function of Cryptococcus neoformans Is Required for its Growth and Virulence

( Jingbo Li ),( Yun C. Chang ),( Chun Hua Wu ),( Jennifer Liu ),( Kyung J. Kwon Chung ),( Sheng He Huang ),( Hiro Shimada ),( Rob Fante ),( Xiaowei Fu ),( Ambrose Jong ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.5

Cryptococcus neoformans is a life-threatening pathogenic yeast that causes devastating meningoencephalitis. The mechanism of cryptococcal brain invasion is largely unknown, and recent studies suggest that its extracellular microvesicles may be involved in the invasion process. The 14-3-3 protein is abundant in the extracellular microvesicles of C. neoformans, and the 14-3-3-GFP fusion has been used as the microvesicle’s marker. However, the physiological role of 14-3-3 has not been explored. In this report, we have found that C. neoformans contains a single 14-3-3 gene that apparently is an essential gene. To explore the functions of 14-3-3, we substituted the promoter region of the 14-3-3 with the copper-controllable promoter CTR4. The CTR4 regulatory strain showed an enlarged cell size, drastic changes in morphology, and a decrease in the thickness of the capsule under copper-enriched conditions. Furthermore, the mutant cells produced a lower amount of total proteins in their extracellular microvesicles and reduced adhesion to human brain microvascular endothelial cells in vitro. Proteomic analyses of the protein components under 14-3-3-overexpressed and -suppressed conditions revealed that the 14-3-3 function(s) might be associated with the microvesicle biogenesis. Our results support that 14-3-3 has diverse pertinent roles in both physiology and pathogenesis in C. neoformans. Its gene functions are closely relevant to the pathogenesis of this fungus.

Up-regulation of Insulin-like Growth Factor Binding Protein-3 Is Associated with Brain Metastasis in Lung Adenocarcinoma

Yang, Lishi,Li, Junyang,Fu, Shaozhi,Ren, Peirong,Tang, Juan,Wang, Na,Shi, Xiangxiang,Wu, Jingbo,Lin, Sheng Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.4

The brain is the most common metastatic site of lung adenocarcinoma; however, the mechanism of this selective metastasis remains unclear. We aimed to verify the hypothesis that exposure of tumor cells to the brain microenvironment leads to changes in their gene expression, which promotes their oriented transfer to the brain. A549 and H1299 lung adenocarcinoma cells were exposed to human astrocyte-conditioned medium to simulate the brain microenvironment. Microarray analysis was used to identify differentially expressed genes, which were confirmed by quantitative real-time PCR and western blotting. Knockdown experiments using microRNAs and the overexpression of genes by cell transfection were performed in addition to migration and invasion assays. In vitro findings were confirmed in clinical specimens using immunohistochemistry. We found and confirmed a significant increase in insulin-like growth factor binding protein-3 (IGFBP3) levels. Our results also showed that the up-regulation of IGFBP3 promoted A549 cell epithelial-mesenchymal transition, migration, and invasion, while the knockdown of IGFBP3 resulted in decreased cell motility. We also found that Transforming growth factor-${\beta}$ (TGF-${\beta}$)/Mothers against decapentaplegic homolog 4 (Smad4)-induced epithelial-mesenchymal transition was likely IGFBP3-dependent in A549 cells. Finally, expression of IGFBP3 was significantly elevated in pulmonary cancer tissues and intracranial metastatic tissues. Our data indicate that up-regulation of IGFBP3 might mediate brain metastasis in lung adenocarcinoma, which makes it a potential therapeutic target.

Nrf2 in adipocytes

Zhendi Wang,Zhuo Zuo,Lu Li,Suping Ren,Tianchang Gao,Jingqi Fu,Yongyong Hou,Yanyan Chen,Jingbo Pi 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.3

White adipocytes play a key role in maintainingwhole body energy homeostasis by forming white adiposetissue (WAT). The impairment of WAT formation or WATdysfunction is clearly associated with severe metabolic disorders. Mature adipocytes are derived from differentiatedpreadipocytes and are pivotal in energy storage and metabolism. Nuclear factor erythroid 2-related factor 2 (Nrf2) isa member of a family of CNC-bZIP proteins which exerttheir transcriptional control on genes harboring antioxidantresponse elements (ARE) in partnership with smallmusculoaponeurotic fibrosarcoma proteins. The activationof Nrf2-ARE coordinated by specific repressor Kelch-likeECH-associated protein 1 (Keap1) regulates networks ofgenes controlling diverse homeostatic processes involvingadaptive antioxidant response and detoxification amongmany other adaptive responses. Interestingly, accumulatingevidence indicates that Nrf2 may act as a transcription factorin regulating the formation and function of adipose tissues,including adipogenesis, lipid metabolism and insulin sensitivity. In this mini-review, an overview on the distinct rolesof Nrf2 in adipocytes is provided. While highlighting the regulatory role of Nrf2 in adipogenesis, recent key findingson Nrf2 in insulin signal transduction and energy metabolismof adipocytes are also summarized.