Combined transcriptomic and proteomic analysis of flubendiamide resistance in Plutella xylostella

Li Jing‐Jing,Jin Ming‐Hui,Wang Nian‐Meng,Yu Qi‐Tong,Shang Ze‐Yu,Xue Chao‐Bin 한국곤충학회 2020 Entomological Research Vol.50 No.10

Diamondback moth (DBM), Plutella xylostella, is an important pest of crucifers worldwide. The extensive use of diamide insecticides has led to DBM resistance in the world, and this presents a serious threat to vegetable production. In the present study, transcriptomic and proteomic analyses were combined to investigate the potential flubendiamide‐resistance mechanism in DBM. The lab‐selected (Rh) and field‐collected (Rb) flubendiamide‐resistant lines of P. xylostella with resistance ratios of 1889.92‐fold and 1250.97‐fold, respectively, were used, as well as a lab‐reared flubendiamide‐susceptible line (S). Compared with the S group, the transcriptomic analysis revealed 151 upregulated and 287 downregulated gene messengers in the Rh group and 432 upregulated and 565 downregulated gene messengers in the Rb group. The most frequently enriched pathways of differentially expressed genes (DEGs) were mainly involved in metabolic pathways. Metabolism related genes, including two P450, two ABC transporters, and three trypsins, were upregulated in the Rh line. Additionally, some P450 genes, trypsin, juvenile hormone (JH), and mucin genes were also upregulated in the Rb line. In proteomic analysis comparisons with the S group, there were 78 upregulated and 90 downregulated proteins in the Rh group and 221 upregulated and 155 downregulated proteins in the Rb group. Further analyses found that three CYP and 11 CYP proteins were over‐expressed in Rh and Rb lines, respectively. Four glutathione S‐transferase (GST) and four UGTs were over‐expressed in Rb line. So, we deduced that the detoxification metabolism may be the main mechanism of flubendiamide resistance in P. xylostella.

Strengthening flotation enrichment of Pb(Ⅱ)-activated scheelite with N-[(3-hydroxyamino)-propoxy]-N-hexyl dithiocarbamate

Jing Qi,Gang Zhao,Sheng Liu,Wei Chen,Guangyi Liu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

To improve the flotation recovery of tungsten minerals was of an urgent issue. In this paper, N-[(3-hydroxyamino)-propoxy]-N-hexyl dithiocarbamate (HAHD) was designed as a powerful collector toenrich and separate scheelite particles. In comparison to benzohydroxamic acid (BHA), HAHD dramaticallypromoted the floatability of the Pb(Ⅱ)-activated scheelite. Meanwhile, HAHD exhibited a competentselectivity for flotation separation of scheelite from calcite. The findings of zeta potential, contact angle,FTIR, UV and XPS deduced that both HAHD’s dithiocarbamate and hydroxamate groups were of the reactivecenters to the Pb(Ⅱ)-activated scheelite where the surface HAHD-Pb(Ⅱ) complex was generated. Theintroduction of the dithiocarbamate group had a limited influence on HAHD’s flotation affinity towardscalcite, while strengthened HAHD aggregation on to the Pb(Ⅱ)-activated scheelite surface, the floatabilitydifference between scheelite and calcite significantly increased. Moreover, the ‘‘h-shape” adsorptionmode and hexyl group of HAHD possessed a stronger hydrophobization towards scheelite than thesingle-site adsorption and benzene ring of BHA, returning a higher flotation recovery of scheeliteparticles.

Numerical Analysis on the Dynamics Pneumatic Vibrators

Jing Yang Qi,Guo Zhong Zhang,Bang Feng Wang,Rui Fang Zhang 대한기계학회 1997 Asia-Pacific Vibration Conference Vol.2 No.1

A hemoglobin-based oxygen-carrying biomimetic nanosystem for enhanced chemo-phototherapy and hypoxia alleviation of hepatocellular carcinoma

Jing-Qing Le,Fang Yang,Xun-Huan Song,Ke-Ke Feng,Ling-Wu Tong,Meng-Die Yin,Wen-Zhong Zhang,Ying-Qi Lin,Hui Wu,Jing-Wei Shao 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

Tumor microenvironment is characterized by low pH, high reactive oxygen species and hypoxia, whichprovides a suitable environment for cancer growth. The hypoxia not only elevates tumor angiogenesisand metastasis, but also is responsible for the development of treatment resistance, which graduallybecomes a significant impediment for cancer therapy. Therefore, we developed a biomimetic nanosystemcontaining hemoglobin extracted from red blood cells, chemotherapy drug sorafenib, sensitizer ursolicacid and photosensitizer indocyanine green for enhanced chemo-photo combination therapy of hepatocellularcarcinoma, which could not only enhance the chemotherapy effect of sorafenib bowing to thesensitizing effect of ursolic acid, but also achieved synergetic phototherapy in virtue of indocyaninegreen. Besides, the nanoparticles could effectively delivery exogenous oxygen to tumor site and amelioratethe tumor hypoxic environment with the assistance of hemoglobin. The dual-sensitization drugdelivery system was expected to effectively reduce the resistance of traditional treatment methodsagainst tumor hypoxia, providing a novel prospect for the synergistic hepatocellular carcinomatreatment.

Liaohe National Park based on big data visualization Visitor Perception Study

Qi-Wei Jing,Zi-Yang Liu(유자양),Cheng-Kang Zheng 한국컴퓨터정보학회 2023 韓國컴퓨터情報學會論文誌 Vol.28 No.4

국립공원은 세계 자연 보존 연맹(WWF)이 수립한 보호지역 관리 체계의 중요 유형 중 하나이며, 또한 자연 및 문화 유산의 효과적인 보호와 지속적인 이용을 실현하는 세계 각국의 관리 모델이다. 이러한 공원은 보호, 과학 연구, 교육, 레크리에이션 및 지역 개발을 비롯한 중요한 역할을 담당하다. 대용량 데이터의 배경 아래, 본 연구는 전 세계 연안 습지의 대표적인 대상인 중국 랴오하 국립공원을 사례 지역으로 삼아 파이썬 기술을 사용하여 중국의 주요 관광 OTA 사이트 중 하나인 망픈웨이(Mafengwo), 셰어이(Gonglve), 큐난우(Chujingyou), 메이툰(Meituan) 및 대중점평넷(Dianping)의 관광객 여행기와 댓글을 데이터 소스로 수집하였다. 텍스트 시간 범위는 2015년부터 2022년까지이며, 총 2,998개의 댓글과 166,588개의 단어를 포함하다. ROST 콘텐츠 마이닝 및 Gephi 소프트웨어를 사용하여 랴오하 국립공원 방문객의 만족도, 인지 과정, 공선 네트워크, 감정 성향 등을 시각적 분석하였다. 결과는 다음과 같다. 야생 동물 및 식물 자원, 강과 바다가 결합 된 자연 경관, 습지 생태는 랴오하 국립공원 방문객의 인식에서 충분히 반영되었다. 방문객은 랴오하 국립공원에 대해 강한 긍정적인 감정을 가지고 있지만, 시설 서비스, 대중교육, 방문객 참여 경험 등에서 여전히 개선할 여지가 있다. National parks are one of the important types of protected area management systems established by IUCN and a management model for implementing effective conservation and sustainable use of natural and cultural heritage in countries around the world, and they assume important roles in conservation, scientific research, education, recreation and driving community development. In the context of big data, this study takes Chinas Liaohe National Park, a typical representative of global coastal wetlands, as a case study, and using Python technology to collect tourists travelogues and reviews from major OTA websites in China as a source. The text spans from 2015 to 2022 and contains 2998 reviews with 166,588 words in total. The results show that wildlife resources, natural landscape, wetland ecology and the fishing and hunting culture of northern China are fully reflected in the perceptions of visitors to Liaohe National Park; visitors have strong positive feelings toward Liaohe National Park, but there is still much room for improvement in supporting services and facilities, public education and visitor experience and participation.

Identification of differentially expressed miRNAs after Lactobacillus reuteri treatment in the ileum mucosa of piglets

Qi Wang,Qian Sun,Jing Wang,Xiaoyu Qiu,Renli Qi,Jinxiu Huang 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Lactobacillus reuteri I5007 possesses many excellent probiotic characteristics in piglets. miRNA plays importantrole in host-microbiota interactions, but the mechanism by which L. reuteri I5007 regulates intestinal function throughits influence on miRNA expression is unknown. Objective This study analyzed the miRNA expression patterns in the ileum mucosa tissue of piglets by L. reuteri I5007treatment, aim to clarify its molecular mechanism for regulating intestinal function through miRNA. Methods Neonatal piglets were orally administered L. reuteri I5007 or a placebo daily starting on day 1, and differentialexpression of ileal miRNAs was analyzed at 10 and 20 days of age by small RNA sequencing. Results 361 known porcine miRNAs were identified, and ten miRNAs were highly expressed in the ileum mucosa in bothtreatments. Nineteen differentially expressed (DE) miRNAs were identified in response to L. reuteri treatment, and four DEmiRNAs (ssc-miR-196a, -196b-5p, -1285 and -10386) were differentially expressed at both time points. The KEGG pathwayanalyses showed the targets of 19 DE miRNAs were involved in 63 significantly enriched pathways, including the PI3KAktand MAPK pathways, which were confirmed to play important roles in probiotic-host communication. L. reuteri I5007exerted anti-inflammatory effects by influencing the levels of inflammatory cytokines. Suppressor of cytokine signalling 4gene was the target gene of ssc-miR-196a/-196b-5p, overexpression of ssc-miR-196a/-196b-5p downregulated the mRNAexpression of IL-1β and TNFα in IPEC-J2 cells. Conclusion Our study provides new insight into the role of miRNAs in the intestinal function of piglets after L. reuteriI5007 treatment.

Combustion Simulation of Variable Altitude Turbocharged Diesel Engine Using SVM

Jing Qi,Luo Qingguo,Gui Yong,Liu Hongbin 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.4

The combustion characteristics of a turbocharged diesel engine in a plateau environment have always been a focus of research on diesel engine power recovery. However, the influence of altitude on the empirical parameters of the combustion model has rarely been addressed in the literature. This paper presents a zero-dimensional combustion modeling method based on support vector machine (SVM). Taking a diesel engine as an example, the heat release rate data under different working conditions were measured on the test bench of a diesel engine at variable altitude. An SVM model was constructed and trained to predict the empirical parameters of the combustion model under varying altitude conditions, and the working process of the diesel engine was simulated. The results show that the working process simulation model with SVM prediction has better predictability. When the altitude increases, the average cylinder pressure and maximum pressure both decrease, the cylinder temperature and maximum temperature both increase, the combustion start point lags, and the combustion duration increases.

MiR-188-5p regulates the proliferation and differentiation of goat skeletal muscle satellite cells by targeting calcium/calmodulin dependent protein kinase II beta

Jing Jing,Sihuan Zhang,Jinbo Wei,Yuhang Yang,Qi Zheng,Cuiyun Zhu,Shuang Li,Hongguo Cao,Fugui Fang,Yong Liu,Ying-hui Ling Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.12

Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. The changes of differentiation marker gene expression were detected by qPCR method. Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. Target gene prediction and dual luciferase assays showed that miR-188-5p could target the 3'untranslated region of the calcium/calmodulin dependent protein kinase II beta (CAMK2B) gene and inhibit luciferase activity. Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. This study will provide a theoretical reference for future studies on the molecular mechanisms of skeletal muscle development in goats.

A Mutation in <i>PGM2</i> Causing Inefficient Galactose Metabolism in the Probiotic Yeast <i>Saccharomyces boulardii</i>

Liu, Jing-Jing,Zhang, Guo-Chang,Kong, In Iok,Yun, Eun Ju,Zheng, Jia-Qi,Kweon, Dae-Hyuk,Jin, Yong-Su American Society for Microbiology 2018 Applied and environmental microbiology Vol.84 No.10

<P>IMPORTANCE Saccharomyces boulardii is a probiotic yeast strain capable of preventing and treating diarrheal diseases. However, the genetics and metabolism of this yeast are largely unexplored. In particular, molecular mechanisms underlying the inefficient galactose metabolism of S. boulardii remain unknown. Our study reports that a point mutation in PGM2, which codes for phosphoglucomutase, is responsible for inferior galactose utilization by S. boulardii. After correction of the mutated PGM2 via genome editing, the resulting strain was able to use galactose faster than a parental strain. While the PGM2 mutation made the yeast use galactose slowly, investigation of the genomic sequencing data of other S. boulardii strains revealed that the PGM2 mutation is evolutionarily conserved. Interestingly, the PGM2 mutation was beneficial for growth at a higher temperature on glucose. We speculate that the PGM2 mutation was enriched due to selection of S. boulardii in the natural habitat (sugar-rich fruits in tropical areas).</P>

Polymorphisms in TYMS for Prediction of Capecitabine-Induced Hand-Foot Syndrome in Chinese Patients with Colorectal Cancer

Si-Qi Dong,Tong-Min Wang,Jiang-Bo Zhang,Yong-Qiao He,Wen-Qiong Xue,Zi-Yi Wu,Da-Wei Yang,Lian-Jing Cao,Jing-Wen Huang,Xi-Zhao Li,Pei-Fen Zhang,Xiao-Hui Zheng,Wei-Hua Jia 대한암학회 2021 Cancer Research and Treatment Vol.53 No.3

Purpose Capecitabine is an extensively used oral prodrug of 5-fluorouracil in treatment of colon cancer and is known to cause hand-foot syndrome (HFS). As the target enzyme for capecitabine, thymidylate synthase (TYMS) plays a key role for 5-fluorouracil metabolism and has been associated with some side effects caused by capecitabine. The aim of our study is to identify the possible genetic predictors of capecitabine-induced HFS (CAP-HFS) in Chinese colorectal cancer patients.Materials and Methods Whole exons of TYMS were sequenced for 288 extreme phenotype HFS patients, including 144 severe or early-onset (first 2 cycles) moderate HFS extreme cases and 144 extreme controls with no reported HFS. The associations between polymorphisms and CAP-HFS were analyzed using logistic regression under an additive model.Results We identified a novel risk mutation (c.1A>G, chr18:657743), was associated with severe HFS in an extreme case who was affected during the first cycle of treatment. Moreover, we identified three new variants, rs3786362, rs699517, rs2790, and two previously reported variants, 5’VNTR 2R/3R and 3′-untranslated region 6-bp ins-del, which were significantly associated with CAP-HFS (p < 0.05). In silico analysis revealed that the effect of these polymorphisms in the TYMS region on the development of HFS might not be restricted solely to the regulation of TYMS expression, but also the TYMS catalytic activity through the indirect effect on ENOSF1 expression.Conclusion This study identified new polymorphisms in TYMS gene significantly associated with CAP-HFS, which may serve as useful genetic predictors for CAP-HFS and help to elucidate the underlying mechanism of HFS.