COMPLETELY GENERALIZED MILDLY NONLINEAR COMPLEMENTARITY PROBLEMS FOR FUZZY MAPPINGS

Huang, Nan-Jing,Zhang, Wen-Bin 한국전산응용수학회 1997 Journal of applied mathematics & informatics Vol.4 No.2

In this paper we introduce and study a new class of completely generalized mildly nonlinear complementarity problems for fuzzy mappings and construct some new iterative algorithms. We also show the existence of solution and the convergence of iterative sequences generated by the algorithm. Our results extend some recent results of Noor, Chang and huang.

Selecting and Identifying Lactic Acid Bacteria in Sourdough and Their Effect on Sourdough Physical and Chemical Properties

( Jing Han Huang ),( Syue Fong Lai ),( Wen Fu Wu ),( Chen Wei Huang ),( Ying Chen Lu ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

Sourdough is traditionally used as the leavening agent for bread making and a potential source of probiotics. In this study, sourdoughs from seven restaurants in Taiwan were collected. The lactic acid bacteria strains were isolated and identified by 16S rDNA sequencing from these sourdoughs. The following lactic acid bacteria species were identified: Lactobacillus pentosus, Lactobacillus paracasei, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus sanfranciscensis. Five lactic acid bacteria strains, Lactobacillus paracasei LYC1242, Lactobacillus casei LYC1244, Lactobacillus plantarum LYC1245 and Lactobacillus casei LYC1246, were selected and singly used to ferment wheat flour. The pH value of various sourdoughs decreased to pH3 from pH5 and kelp at pH3. The titratable acidity and viscosity of various sourdoughs increased significantly compared to spontaneous fermented flour. However, there is no significant difference in titratable acidity between various sourdoughs. The sourdough fermented with L. casei LYC1244 had highest cell count. These studies provided preliminary results for choosing the best lactic acid bacteria starter strain to develop probiotic sourdough bread.

Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides

Huang, Mei-Juan,Fei, Jing-Jing,Salam, Nimaichand,Kim, Chang-Jin,Hozzein, Wael N.,Xiao, Min,Huang, Hai-Quan,Li, Wen-Jun Springer-Verlag 2016 Archives of microbiology Vol.198 No.8

<P>An actinomycete strain, designated YIM T102(T), was isolated from the rhizospheric soil of Psammosilene tunicoides W. C. Wu et C. Y. Wu collected from Lijiang, Yunnan Province, China. The taxonomic position of the new isolate was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM T102(T) belongs to the genus Streptomyces. Strain YIM T102(T) was most closely related to Streptomyces eurocidicus NRRL B-1676(T) with a pairwise 16S rRNA gene sequence similarity of 98.9 %. However, DNA-DNA relatedness value between strain YIM T102(T) and S. eurocidicus NBRC 13491(T) was found to be 37.8 +/- 1.8 %. The menaquinone composition detected for strain YIM T102(T) was MK-9 (H-6) and MK-9 (H-8), while the major fatty acids were summed feature 4 (38.0 %), anteiso-C-15:0 (13.1 %), iso-C-16:0 (10.1 %), summed feature 3 (9.8 %) and C-16:0 (9.0 %) and iso-C-15:0 (5.2 %). The whole-cell hydrolysates contained galactose, glucose, ribose and mannose, along with ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 70.7 mol%. Strain YIM T102(T) also exhibited antagonistic activity against Alternaria alternata, Alternaria brassicae and Colletotrichum nicotianae Averna, based on the findings from the comparative analyses of phenotypic and genotypic characteristics; it is proposed that strain YIM T102 represents a novel species of the genus Streptomyces, for which the name Streptomyces zhihengii sp. nov. is proposed. The type strain is YIM T102(T) (=KCTC 39115(T) = DSM 42176(T) = CGMCC 4.7248(T)).</P>

Comparative Study of Bioactive Constituents in Crude and Processed Glycyrrhizae radix and Their Respective Metabolic Profiles in Gastrointestinal Tract In Vitro by HPLC-DAD and HPLC-ESI/MS Analyses

Wen-wu Huang,Xiaobo Li,Meng Yue Wang,Hai-ming Shi,Ying Peng,Chong-sheng Peng,Min Zhang,Yue Li,Jing Lu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.11

Two HPLC methods with diode array detection (HPLC-DAD) and electrospray ionization-mass spectrometry (HPLC-ESI/MS), respectively, were developed to investigate the differences of chemical constituents and their metabolism in gastrointestinal tract in vitro between two decoctions of crude and processed Glycyrrhizae radix. Total of eleven constituents (liquiritin apioside, liquiritin, licuraside, isoliquiritin, ononin, glycyrrhizin, liquiritigenin-7,4'-diglucoside, licorice saponin A3, 22β-acetoxylglycyrrhizic acid, licorice saponin G2, and yunganoside E2) were identified in the two decoctions, whereas lower contents of these constituents were usually found in the decoction of processed G. radix. Furthermore, these constituents were metabolized into their respective aglycons in human intestinal bacteria juice, and the metabolism ratios were all higher in processed G. radix decoction. No change was found in artificial gastric or intestinal juice. This study revealed that the processing can alter the contents of main constituents in crude G. radix and their metabolism in gastrointestinal tract, in which intestinal bacteria play an important role in the metabolism of licorice constituents.

Mucin2 is Required for Probiotic Agents-Mediated Blocking Effects on Meningitic E. coli-Induced Pathogenicities

Jing Yi Yu,Xiao Long He,Santhosh Puthiyakunnon,Liang Peng,Yan Li,Li Sha Wu,Wen Ling Peng,Ya Zhang,Jie Gao,Yao Yuan Zhang,Swapna Boddu,Min Long,Hong Cao,Sheng He Huang 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.10

ORiginal Article : Tryptase and Protease-Activated Receptor 2 Expression Levels in Irritable Bowel Syndrome

( Wen Jing Liang ),( Guo Zhang ),( He Sheng Luo ),( Lie Xin Liang ),( Dan Huang ),( Fa Can Zhang ) 대한간학회 2016 Gut and Liver Vol.10 No.3

Background/Aims: Previous studies have revealed that mast cells (MCs) may activate the protease-activated receptors and release of neuropeptides involved in the pathogenesis of irritable bowel syndrome (IBS). The levels of proteaseactivated receptor 2 (PAR-2) and tryptase can contribute to understanding the pathogenesis of IBS. Methods: Colonoscopic biopsies were performed of 38 subjects (20 with IBSdiarrhea [IBS-D], eight with IBS-constipation [IBS-C], and 10 healthy volunteers). The mRNA and protein levels of tryptase and PAR-2 were assessed by real-time PCR and Western blot. The levels of vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) were measured by immunohistochemistry, and MCs were counted by toluidine blue staining. Results: Significant increases in the mRNA expression of tryptase (p<0.05, IBS-D, IBS-C vs control) and PAR-2 (p<0.05, IBS-D, IBS-C vs control) and in the tryptase protein level (p<0.05, IBS-D, IBS-C vs control) were detected in IBS. Elevations of MCs, CGRP, VIP and SP (p<0.05, IBS-D vs control) were observed for IBS-D only. Conclusions: Tryptase levels may upregulate the function of PAR- 2, resulting in the release of neuropeptide and they were correlated with clinical symptoms associated with IBS. (Gut Liver 2016;10:382-390)

Molecular cloning, expression profiles and subcellular localization of cyclin B in ovary of the mud crab, Scylla paramamosain

Wen-Xing Li,Hui-Yang Huang,Jing-Ru Huang,Jin-Jin Yu,Jun Ma,Hai-Hui Ye 한국유전학회 2013 Genes & Genomics Vol.35 No.2

A full-length cDNA of cyclin B was isolated from ovary of the mud crab (Scylla paramamosain) in this study. This transcript encodes a polypeptide of 401 amino acids, which is highly homologous to cyclin B protein family. Reverse transcription-PCR (RT-PCR) showed that cyclin B mRNA was expressed at highest levels in ovary of the mud crab. During the ovarian maturation process, realtime RT-PCR revealed that the abundance of cyclin B mRNA increased from the second stage (early-developing stage) to the fourth stage (nearly-ripe stage) and reached the peak level at the fifth stage (ripe stage). This result indicates the identified cyclin B gene might be related to the cell proliferation in ovary, both mitotically and meiotically. Immunohistochemistry showed that cyclin B protein was localized in the cytoplasm of prophase oocytes at the second stage while enriched in the nuclei of pro-metaphase oocytes at the fourth stage. It suggests the tested cyclin B protein might play different roles in ovary at the two stages.

Convenient hydrogel adhesion with crystalline zones

Wen-Tao Chen,Liangpeng Zeng,Ping Li,Yi Liu,Jing-Liang Huang,Hui Guo,Ping Rao,Wei-Hua Li 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

Adhering soft hydrogels to hard substrates is challenging. Existing adhesion approaches mainly rely oninterfacial chemical bonds or physical interactions, which typically require complex fabrication processesor specific surface properties. Herein, we report a convenient approach to achieving effective hydrogeladhesion onto various hard substrates with in-situ formed crystalline zones by heating. Semicrystallizablepoly(vinyl alcohol) hydrogels were directly cast on various subtracts by a dryingswellingprocess, which demonstrates strong adhesion with adhesion energy as high as 890 J/m2. Theadhesion performance demonstrates an obvious positive correlation with the crystalline property. Therefore, the factors determining the crystalline degree, including the polymer type and preparationtemperature, demonstrate remarkable influences on the adhesion performance. Moreover, this adhesionperformance manifests high durability in harsh environments. Benefitting from the strong and durableadhesion performance, the crystalline gels can serve as a tie coating layer to joint various hydrogels,which demonstrate satisfactory antifouling ability. Therefore, this strategy may endow the materials withgreat potential for various practical applications in the future.

Treatment of Growth Plate Injury with Microencapsulated Chondrocytes

Wen-Chao Li,Rui-Jiang Xu,Yi-Long Xu,Jing-Xiang Huang,Yu-Hong Gao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4

Background: Tissue-engineered cartilage has provided a promising method in the treatment of physeal growth arrest. This study was designed to investigate transplantation of microencapsulated allogeneic chondrocytes to treat the injured growth plate. Methods: Allogeneic chondrocytes were encapsulated within alginate-polylysinealginate semipermeable membranes. Microencapsulated chondrocytes co-cultured with Bone Mesenchymal Stem Cells (BMSCs) were evaluated whether it could promote chondrogenesis of BMSCs. An experiment model of an injured growth plate was made by resecting the lateral half of the right distal femur physis in rabbits. Microencapsulated allogeneic chondrocytes, allogeneic chondrocytes as well as empty microcapsules were grafted into growth plate defects of 6-week-old rabbits. Histological and radiographic examinations were examined after transplantation up to 16 weeks. Results: The histological study showed that BMSCs co-cultured with microencapsulated chondrocytes could produce GAG and II collagen similarly with chondrocytes. Angular deformity and length discrepancy in the group with microencapsulated allogeneic chondrocytes were less than those in other groups (p < 0.001). The histological study confirmed the viability of microencapsulated chondrocytes at 16 weeks postoperatively. The neogenetic chondrocytes of columnar arrangement have been found in the growth plate defect to prevent early ossification and closure of the growth plate. Conclusions: The histological study confirmed the viability of microencapsulated chondrocytes at 16 weeks postoperatively. The neogenetic chondrocytes of columnar arrangement have been found in the growth plate defect to prevent early ossification and closure of the growth plate.

A BP Neural Network Based on Improved PSO for Increasing Current Efficiency of Copper Electrowinning

Wu Jing,Cheng Yan-Ming,Liu Cheng,Lee Il-Kyoo,Cha Jae-Sang,Huang Wen-Lin 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.3

In this paper, to improve the current effi ciency in the copper electrowinning process is taken as the research objective. In the traditional production process, sulfate ion concentration, copper ion concentration and current density are carried out according to the empirical value, which cannot ensure the current effi ciency to reach the optimal level. Therefore, fi rstly, this paper proposes a BP neural network model to improve the current effi ciency according to the relationships between sulfate ion concentration, copper ion concentration, current density and the established BP neural network model is trained by using real data from the enterprise. The simulation results indicate that there is a defi nite error between the predicted current effi ciency and corresponding to the current effi ciency measured at the production site. It is proposed that the BP neural network improved by the improved PSO to further improve the prediction accuracy of the BP neural network. Simulation results indicate that the prediction error of the current effi ciency is greatly reduced that meets the accuracy requirements. On the premise of guaranteeing the quality of copper electrowinning, the current density, sulfate ion concentration and copper ion concentration corresponding to the maximum current effi ciency accurately predicted by this method can be respectively adjusted in real-time in the copper electrowinning process, which realizes the optimization of current effi ciency in the process of copper electrowinning under the background of low carbon and environmental protection.