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Wei Tong,Qiang He,Xiao-Qiang Wang,Min-Young Yoon,Won-Hee Ra,Feng Peng Li,Jie Yu,Win Htet Oo,Sun-Kyung Min,Buung Choi,Eun-Beom Heo,Byoung-Kook Yun,Kyu-Won Kim,Tae-Sung Kim,Chang-Yong Lee,Yong-Jin Park 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Although the overall structure of the chloroplast genome is generally conserved, a number of sequence variations have been identified, which are valuable for plant population and evolutionary studies. Here, we constructed a chloroplast variation map of 30 landrace rice strains of Korean origin, using the Oryza rufipogon chloroplast genome (Genbank: NC_017835) as a reference. Differential distribution of single nucleotide polymorphisms (SNPs) and indels across the rice chloroplast genome is suggestive of a region-specific variation. Population structure clustering revealed the existence of two clear subgroups (indica and japonica) and an admixture group (aus). Phylogenetic analysis of the 30 landrace rice strains and six rice chloroplast references suggested and supported independent evolution of O. sativa indica and japonica. Interestingly, two “aus” type accessions, which were thought to be indica type, shared a closer relationship with the japonica type. One hypothesis is that “Korean aus” was intentionally introduced and may have obtained japonica chloroplasts during cultivation. We also calculated the nucleotide diversity of 30 accessions and compared to six rice chloroplast references, which shown a higher diversity in the indica and aus groups than in the japonica group in lower level substitution diversity.
Laser Cladding Novel NiCrSiFeBW–CeO2 Coating with Both High Wear and Corrosion Resistance
Min Guo,Suiyuan Chen,Fanmin Shang,Jing Liang,Tong Cui,Changsheng Liu,Mei Wang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.8
In order to obtain the high wear- and corrosion-resistant nickel based alloy coatings for laser remanufacturing fretting damaged metal parts which are serviced under high-temperature corrosion and wear conditions, a novel NiCrSiFeBW–CeO2alloy powder was designed by increasing the content of B and Si, adding tungsten and CeO2 using JMatpro software onthe basis of Ni60 alloy powder composition, and the NiCrSiFeBW–CeO2 coating was successfully cladded on 45# steelunder diferent laser energy area densities. The microstructure, wear and corrosion behaviors of the NiCrSiFeBW–CeO2coatings were systematically studied. The results show that novel NiCrSiFeBW–CeO2 coating produced by laser claddingnot only has no cracks but also has both high wear resistance and corrosion resistance due to some ultra-fne compoundparticle phases in situ generated in its structure. Among these phases, the B3Cr5, CrB4, (Cr, Ni)3C2, Cr7C3, W3Cr12Si5 and(Fe, Ni)5Si3 played a signifcant role in reinforcing the wear resistance of the coating, while the B3Cr5, W3Cr12Si5 and CrB4enhanced the corrosion resistance of the coating. The novel NiCrSiFeBW–CeO2 coating prepared under 100 J/mm2EADhas the best comprehensive performance, the wear loss is 7.53×10−5 mm3/N, the Ecorr is − 0.1738 V. Compared with Ni60alloy coating, the novel Ni-based coating not only has a better laser cladded formability but also similar wear resistance andbetter corrosion-resistance. It provides a reference for repairing fretting damaged metal parts by laser cladding the nickelbased coating with high wear and corrosion resistance.
Wang, Jun-Xi,Zhang, Ying-Yao,Yu, Xue-Min,Jin, Tong,Pan, Xin-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3
Purpose: Centromere protein H (CENP-H) and Ki67 are overexpressed in some malignancies, but whether they are predictors of survival after primary resection for hypopharyngeal squamous cell carcinoma (HSCC) remains unknown. Methods: We assessed immunohistochemical expression of CENP-H and Ki67 in 112 HSCC specimens collected between March 2003 and March 2005 for analysis by clinical characteristics. The Kaplan-Meier method was used to analyze relapse-free survival and logistic multivariate regression to determine risk factors of relapse-free survival. Cholecystokinin octapeptide assays and flow cytometry were used to examine cell proliferation and apoptosis after siRNA inhibition of CENP-H in HSCC cells. Results: Overall, 50 (44.6%) HSCC specimens showed upregulated CENP-H expression and 69 (61.6%) upregulated Ki67. An increased CENP-H protein level was associated with advanced cancer stage and alcohol history (P=0.012 and P=0.048, respectively) but an increased Ki67 protein level only with advanced cancer stage (P=0.021). Increased CENP-H or Ki67 were associated with short relapse-free survival (P<0.001 or P=0.009, respectively) and were independent predictors of relapse-free survival (P=0.001 and P=0.018, respectively). siRNA knockdown of CENP-H mRNA inhibited cell proliferation and promoted cancer cell apoptosis in vitro. Conclusions: Upregulated CENP-H and Ki67 levels are significantly associated with short relapse-free survival in HSCC. These factors may be predictors of a relapsing phenotype in HSSC cases.
Xue, Tong,Wang, Xin,Kwak, Sang Kyu,Lee, Jong-Min American Chemical Society 2013 INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH - Vol.52 No.14
<P>Mesoporous dual layers of polyaniline (PANI) supported selenium–tellurium (Se<SUB>0.5</SUB>Te<SUB>0.5</SUB>) films have been synthesized from lyotropic liquid crystalline (LLC) template with Brij56 surfactant. The optical texture of LLC is observed by polarized optical microscopy (POM). The structure of the dual-layer film is characterized by low-angle and wide-angle X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The band gap of Se<SUB>0.5</SUB>Te<SUB>0.5</SUB>/PANI dual-layer films was estimated to be 0.9 eV, and the band gap of Se<SUB>0.5</SUB>Te<SUB>0.5</SUB> films was calculated to be 1.4 eV, indicating that the incorporation of PANI into Se<SUB>0.5</SUB>Te<SUB>0.5</SUB> enhances its electron transfer.</P>
Su Tong,Zhang Zhe,Chen Yu,Wang Yun,Li Yumei,Xu Min,Wang Jian,Li Jing,Tian Xinping,Jin Zhengyu 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.4
Objective: To evaluate the image quality of novel dark-blood computed tomography angiography (CTA) imaging combined with deep learning reconstruction (DLR) compared to delayed-phase CTA images with hybrid iterative reconstruction (HIR), to visualize the cervical artery wall in patients with Takayasu arteritis (TAK). Materials and Methods: This prospective study continuously recruited 53 patients with TAK (mean age: 33.8 ± 10.2 years; 49 females) between January and July 2022 who underwent head-neck CTA scans. The arterial- and delayed-phase images were reconstructed using HIR and DLR. Subtracted images of the arterial-phase from the delayed-phase were then added to the original delayed-phase using a denoising filter to generate the final-dark-blood images. Qualitative image quality scores and quantitative parameters were obtained and compared among the three groups of images: Delayed-HIR, Dark-blood-HIR, and Dark-blood-DLR. Results: Compared to Delayed-HIR, Dark-blood-HIR images demonstrated higher qualitative scores in terms of vascular wall visualization and diagnostic confidence index (all P < 0.001). These qualitative scores further improved after applying DLR (Dark-blood-DLR compared to Dark-blood-HIR, all P < 0.001). Dark-blood DLR also showed higher scores for overall image noise than Dark-blood-HIR (P < 0.001). In the quantitative analysis, the contrast-to-noise ratio (CNR) values between the vessel wall and lumen for the bilateral common carotid arteries and brachiocephalic trunk were significantly higher on Darkblood- HIR images than on Delayed-HIR images (all P < 0.05). The CNR values were significantly higher for Dark-blood-DLR than for Dark-blood-HIR in all cervical arteries (all P < 0.001). Conclusion: Compared with Delayed-HIR CTA, the dark-blood method combined with DLR improved CTA image quality and enhanced visualization of the cervical artery wall in patients with TAK.
Expression of IER3 in Primary Hepatocarcinoma: Correlation with Clinicopathological Parameters
Liu, Zhong,Wang, Xin-Mei,Jia, Tong-Fu,Zhai, Yi,Sun, Ling-Yan,Cheng, Yu-Ping,Zhang, Yue-Min,Liu, Shi-Hai,Liang, Jun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2
Background: Studies indicate the immediate early response gene 3 (IER3) is involved in many biological processes. Recently, it was discovered that IER3 plays an important role in tumorigenesis and tumor progression. Thus it may be a valuable biomarker in tumor. This study was designed to investigate the expression status of IER3 in primary hepatocarcinoma (PHC) and correlation with clinicopathological parameters. Materials and Methods: Real-time PCR was performed to evaluate the expression levels of IER3 in 62 pathologically diagnosed human PHC specimens. Results: A statistically significant association was disclosed between the expression of IER3 and P53 mutant protein (short for P53), Ki-67, EGFR and the biggest diameter, differentiation grade of tumor. Conclusions: This work is the first to shed light on the potential clinical usefulness of IER3, as an efficient tumor biomarker in PHC.
Jang, Ji Yong,Min, Ji Hyun,Chae, Yun Hee,Baek, Jin Young,Wang, Su Bin,Park, Su Jin,Oh, Goo Taeg,Lee, Sang-Hak,Ho, Ye-Shih,Chang, Tong-Shin Mary Ann Liebert 2014 ANTIOXIDANTS AND REDOX SIGNALING Vol.20 No.16
<P>The collagen-stimulated generation of reactive oxygen species (ROS) regulates signal transduction in platelets, although the mechanism is unclear. The major targets of ROS include protein tyrosine phosphatases (PTPs). ROS-mediated oxidation of the active cysteine site in PTPs abrogates the PTP catalytic activity. The aim of this study was to elucidate whether collagen-induced ROS generation leads to PTP oxidation, which promotes platelet stimulation.</P>
Simin Liu,Weiwei Chen,Min Wang,Tong Wu,Lingli Dong,Chu Pan,Wenzhen Zhu 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.3
Objective: To evaluate the secretory function of parotid glands by dynamic magnetic resonance (MR) sialography and determine the clinical performance of this technique in diagnosing and evaluating Sjögren’s syndrome (SS) patients. Materials and Methods: This study enrolled 29 healthy volunteers (25 women and 4 men; mean age, 34.8 ± 6.3 years; age range, 26–47 years) and 25 primary SS (pSS) patients (23 women and 2 men; mean age, 37.7 ± 7.9 years; age range, 25–50 years) with decreased secretory function. The volume of the parotid gland ducts was precisely measured for both groups at single pre- and 6 post-gustatory-stimulated phases. Time-dependent volume change ratio curves were generated, four parameters were derived from the curves: the slope of the increase in the first post-stimulation phase (slope1st), the peak value, the time-to-peak, the total saliva secretion post-stimulation. All values were used to quantitatively evaluate the secretory function of the parotid gland. The repeated measurement analysis, Mann-Whitney U test and receiver operating characteristic curve were applied. Results: Time-dependent volume change ratio curves demonstrated that there is a statistically significant difference between the two groups (F = 8.750; p = 0.005). A quickly increasing curve was shown in the volunteer group, whereas a slowly increasing curve was shown in the pSS patient group. The slope1st, peak value and total saliva secretion post-stimulation of the patient group were significantly lower than those of the volunteer group (p = 0.005, p = 0.003, and p = 0.002, respectively). The timeto-peak between the two groups was not significantly different (p = 0.383). The slope1st can be used as a discriminator to diagnose SS patients (p = 0.015; odds ratio = 4.234; area under the curve = 0.726). Conclusion: Dynamic MR sialography is proven to be an effective method in evaluating salivary gland function and has a great potential in diagnosing and evaluating pSS patients.