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      • Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

        ( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

        In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

      • RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

        박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

        TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

      • SCIESCOPUSKCI등재

        Facile preparation of self-assembled wool-based graphene hydrogels by electron beam irradiation

        Mira Park,Bishweshwar Pant,Jawun Choi,Yong Wan Park,Chohye Lee,Hye Kyoung Shin,Soo-Jin Park4,Hak-Yong Kim 한국탄소학회 2014 Carbon Letters Vol.15 No.2

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        Three dimensional self-assembled graphene hydrogels were easily fabricated by electron beam irradiation (EBI) using an aqueous solution of wool/poly(vinyl alcohol) and graphene oxide (GO). After exposure to various levels of EBI radiation, the highly porous, self-assembled, wool-based graphene hydrogels were characterized using scanning electron microscopy and Fourier-transform infrared spectroscopy; to determine the gel fraction, degree of swelling, gel strength, kinetics-of-swelling analyses and removal of hexavalent chromium (Cr(VI)) from the aqueous solution. X-ray diffraction results confirmed that EBI played a significantly important role in reducing GO to graphene. The adsorption equilibrium of Cr(VI) was reached within 80 min and the adsorption capacity was dramatically increased as the acidity of the initial solution was decreased from pH 5 to 2. Changes in ionic strength did not exert much effect on the adsorption behavior.

      • KCI등재후보

        대량의 각혈로 사망한 장티푸스 1예

        한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

        We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.

      • LED 기생 커패시턴스를 고려한 접합온도 측정 시스템의 개선

        박종연,유진완 江原大學校 産業技術硏究所 2009 産業技術硏究 Vol.29 No.B

        Recently, we have used LEDs to illumination because it has a high luminous efficiency and prolong lifespan. However the light power and lifetime is reduced by junction temperature increment of LED. So it is important to measure the junction temperature accurately. In case of using a electrical method measuring junction temperature of LED. Temperature measurement errors are spontaneously generated because of a parasitic capacitances in LED. In this paper, we proposed a method that reducing LED's parasitic capacitance effects for electrical measurement. It was demonstrated by the experimental result that is more correct than established method.

      • 표고버섯의 저장성 연장에 관한 연구

        박철,윤희진,신완철,최석영 울산대학교 2006 생활과학논문집 Vol.7 No.2

        저농도의 산소를 함유한 기체조성(G108)과 고농도의 이산화탄소를 함유한 기체조성(G604)을 이용하여 표고버섯의 저장성 연장 효과를 평가하였다. 표고버섯을 실온(22°C)과 냉장저장(4°C)으로 나누어 저장하면서 중량감소율, pH 변화, 색도(L값), PPO 활성, 유리아미노산 함량을 측정하였다. 실온저장 대조군은 7일 경과 후 중량이 6.3%까지 감소하였으나, G108군과 G604군은 각각 3.0%, 3.2% 감소하였다. pH는 실온저장 대조군에서는 저장 3일째 급격히 감소하다가 7일에는 크게 상승하였다. 반면에 G108과 G604군에서는 pH 변화가 3일까지 변화가 없다가 7일에는 감소하였다. L값은 실온저장 3일에 대조군이 46.4인 반면, G108과 G6O4군은 7일에 각각 76.1, 76.6이었다. 냉장저장 시 대조군의 중량은 7일부터 감소하기 시작하여 35일에는 39.0%까지 감소하였으나, G108과 G6O4군에서는 42일까지 각각 3.1%, 3.0% 감소하였다. 대조군의 pH는 저장 21일까지 크게 감소하다가 증가하기 시작하여 저장 35일째에는 크게 상승하였다. 반면에 G108과 G604군에서는 저장 42일까지 pH변화가 거의 일어나지 않았다. 냉장저장 시 대조군의 L값은 14일 경과 후 큰 폭으로 떨어졌지만, G108군과 G6O4군은 42일까지 별로 감소하지 않았다. PPO 활성은 대조군에서는14일부터는 크게 증가하였으나 G108과 G604 모두 저장 42일까지 거의 증가하지 않았다또한 유리 아미노산 함량도 대조군에서는 크게 감소하였으나 기체치환군에서는 그 감소율이 크게 지연되었다. 이상의 결과로 표고버섯의 저장 시 기체치환으로 버섯의 호흡을 억제함으로써 버섯의 저장성을 연장시킬 수 있음을 알 수 있었다. 실온저장 시 대조군은 1일부터 각종 품질 변화가 일어나는 반면에, 기체치환저장은 3일까지 변화가 일어나지 않았으며, 냉장저장 시에는 대조군이 3일경부터 품질 변화가 일어나는데, 기체치환저장은 14일까지 품질의 큰 변화가 없음을 알 수 있었다. The prolongation of shelf-life of oak mushroom(Lentius edodes) using low oxygen concentration(G108) and high carbon dioxide concentration(G604) was evaluated. The weight loss, pH change, colour(L value), PPO activities and free amino acid contents were measured during 7 days at 22"C or during 42 days at 4'c storage. During 22"C storage the weight loss rate of control was 6.3% at 7 days, but that of G108 and G6O4 were 3.0% and3.2% at 7 days respectively. The pH of control was decreased at 3 days, but increased thereafter, However The pH of G108 and G6O4 were not changed during 3 days and were decreased thereafter. The colour (L value) of control was 46.4 at 3 days, but that of G108and G604 were 76.1, 76.6 during 7 days respectively. During 4"C storage the weigh loss rate of control was 39.0% at 35 days, but that of G108 and G6O4 were little changed during 42 days. The pH of control was decreased at 21 days, but increased after 35 days. The pH of G108 and G604 were not changed during 42 days. The colour (L value) of control was highly decreased at 14 days, but that of G108 and G6O4 were little changed during 42 days. Also, PPO activities of control were continually increased from 14 days, that of G108 and G6O4 however were little changed during 42 days. The contents of free amino acids of control were decreased continuously, but their decreasing rate was delayed in the groups of modified atmosphere packaging. These results suggested that the shelf-life of oak mushroom was prolonged by inhibiting the respiration of that using the modified atmosphere packaging. During 22'c storage the qualities of control were not changed at 1 days, but that of G108 and G6O4 were not changed during 3 days. Also during 4f storage the qualities of control were not changed at 3 days, but that of G108and G6O4 were not changed during 14 days.

      • 고압 수증기 내에서 산화막 형성에 관한 연구

        박경희,안순의,구경완,왕진석 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.2

        This paper shows experimentally that oxide layer on the p-type Si-substrate can grow at low temperature(500℃∼600℃) using high pressure water vapor system. As the result of experiment, oxide layer growth rate is about 0.19Å/min at 500℃, 0.43Å/min at 550℃, 1.2Å/min at 600℃ respectively. So, we know oxide layer growth follows reaction-controlled mechanism in given temperature range. Consequently, granting that oxide layer growth rate increases linearly to temperature over 600℃, we can expect oxide growth rate is 5.2Å/min at 1000℃. High pressure oxidation of silicon is particularly attractive for the thick oxidation of power MOSFET, because thermal oxide layers can grow at relatively low temperature in run times comparable to typical high-temperature. 1 atm conditions. In the condition of higher-temperature and high-pressure steam oxidation. the oxidation time is reduced significantly.

      • SCIESCOPUSKCI등재

        Block of ATP-Sensitive K Channels Expressed in Xenopus Oocytes by Dimethyl Sulfoxide

        Jin Bong Park1,Soo Wan Chae 대한생리학회-대한약리학회 2001 The Korean Journal of Physiology & Pharmacology Vol.5 No.2

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        <P> The effects of dimethyl sulfoxide (DMSO) were studied in two groups of Xenopus oocytes, one expressing ATP sensitive K (KATP) channel comprised of sulfonylurea receptor SUR1 and inwardly rectifying K channel subunit Kir6.2, and the other expressing renal KATP channel ROMK2. At concentrations of 0.3∼10% (vol/vol) DMSO inhibited whole cell Kir6.2/SUR1 currents elicited by bath application of sodium azide (3 mM) in a concentration-dependent manner. The inhibition constant and Hill coefficient were 2.93% and 1.62, respectively. ROMK2 currents, however, was not affected significantly by DMSO. The results support the idea that DMSO inhibits KATP channel expressed in Xenopus oocyte through a protein-specific mechanism(s) that remains to be further elucidated.

      • KCI등재후보

        친수성 프라이머를 이용한 교정용 브라켓 접착시의 전단결합강도에 관한 연구

        박철완,차경석,이진우 대한치과교정학회 2002 대한치과교정학회지 Vol.32 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        본 연구의 목적은 산부식된 법랑질에 수분이나 타액이 오염되어도 적절한 접착력을 얻을 수 있다고 소개된 교정용 친수성 프라이머를 이용한 교정용 브라켓 접착시 타액 오염 정도에 따른 전단결합강도와 접착 파절 양상을 기존의 소수성 프라이머와 비교함으로써 임상적 유용성을 평가하는 것이다. 사람의 소구치를 강철 원통에 교정용 레진으로 포매하여 만든 시편에 기존의 소수성인 Transbond XT primer와 친수성인 Transbond MIP primer 각각에 대하여 광중합형 접착 레진으로 브라켓을 접착시, 인공 타액을 이용한 오염정도에 따른 전단결합강도를 만능시험기로 측정하고, 접착 파절 양상을 stereomicroscope로 관찰하여 다음과 같은 결과를 얻었다. 1. 건조 상태에서 Transbond XT primer와 Transbond MIP primer의 전단결합강도는 유의한 차이가 없었다. 2. 타액 오염시 그정도에 상관없이 Transbond MIP primer는 Transbond XT primer에 비해 유의하게 높은 전단결합 강도를 나타냈다(p<0.001). 3. Transbond MIP primer는 한겹의 타액 오염시 건조 상태와 전단결합강도의 유의한 차이가 없었으나, 두 겹의 타액 오염시에는 유의하게 낮은 결합강도를 나타냈다(p<0.01). 4. 접착 파절 형태는 타액 오염의 정도에 따라 평균 접착제 잔류 지수가 낮아지는 경향을 나타냈다. Transbond MIP primer는 타액 오염시에도 반 이상이 브라켓-레진 계면에서의 파절을 보였으나, Transbond XT primer는 타액 오염시 대부분의 경우 레진-법랑질 계면에서의 파절을 나타냈다. 이상의 실험 결과, 교정용 친수성인 Transbond MIP primer는 수분 조절이 어려운 임상 상황에서 적절한 결합강도를 얻을 수 있는 좋은 방법으로 생각된다. The purpose of this study was to evaluate the clinical effectiveness of hydrophilic primer, which claim to retain adequate bond strength on moistened enamel resulting from moisture or saliva contamination, by comparing the shear bond strength and adhesive failure patterns of brackets bonded using hydrophilic primer and conventional hydrophobic primer. Brackets were bonded to human premolars embedded in metal cylinders utilizing light cured adhesive, primed with either a hydrophilic primer(Transbond MIP primer) or a conventional hydrophpbic primer(Transbond XT primer). Each sample was exposed to varying degrees of artificial saliva contamination during the priming process. The shear bond strength was measures using a universal testing machine, and the adhesive failure patterns after debonding were visually examined by stereomicroscope and assessed using the adhesive remnant index(ARI). The results were as follows : 1. In dry conditions, no significant differences in shear bond strength between Transbond MIP and Transbond XT primers were found. 2. Trandbond MIP primer exhibited a significantly higher shear bond stregth than Transbond XT primer in saliva-contaminated conditions, regardless of the degree of contamination. 3. When contaminated with one coat of saliva, Transbond MIP primer did not exhibit significant differences in shear bond strength compared to the dry condition. When contaminated with two coats of saliva, Transbond MIP primer exhibited a significantly lower shear bond strength compared to the dry condition. 4. The adhesive remnant index of the adhesive failure pattern had a tendency to decrease, as the degree of saliva contamination increased .Bracket-adhesive interface failure was observed in more than half of the saliva contaminated samples utilizing Transbond MIP primer, whereas the bond failure sites of the Transbond XT primer samples occurred almost exclusively at the adhesive-enamel interface in saliva-contaminated conditions. The results of this study suggest that in cases where moisture control is difficult, Transbond MIP primer is an effective alternative to conventional hydrophobic primers.

      • KCI등재

        강원대학교 연습림내 蓮葉山과 九節山地域의 植物相에 관한 硏究

        朴完根,廉圭眞,朴善貞 江原大學校 森林科學硏究所 1997 Journal of Forest Science Vol.13 No.-

        要約강원대학교 연습림의 연엽산과 구절산 지역을 대상으로 植物相을 조사한 결과, 管束植物資源은 98科 345屬 551種 2亞種 88變種 10品種 2雜種으로 총 653분류군이 분포하는 것으로 나타났다. 이들 所産植物중 韓國特産植物은 20種類, 그리고 稀貴 및 滅種危機植物은 18種類가 분포하였으며, 自然生態系에 예측할 수 없는 영향을 초래하는 歸化植物은 27種類가 출현하였다. 귀화식물 種類수에 의한 自然破壞度(UI)는 약 12.4%로 나타났다. 또한 所産植物들의 有用度는 食用資源(42.1%), 藥用資源(33.8%), 牧草用(28.8%), 觀賞資源(11.8%), 木材資源(5.2%), 纖維資源(1.4%), 工業用(1.1%) 등의 순으로 나타났으며, 아직 용도가 밝혀지지 않은 植物資源도 140種類(21.4%)로 나타났다.ABSTRACTThis study was to survey the flora of resources vascular plants in the Experiment Forests of Kangwon National University. Resources vascular plants were composed of 98 families, 345 genera, 551 species, 2 subspecies, 88 varieties, 10 formae, and 2 hybrid, totaling 653 taxa. Among the investigated resources plants, Korean endemic plants and rare or endangered plants were distributed 20 taxa and 18 taxa, respectively. There were 27 taxa of Naturalized plants in this study area, which would have unpredictable influence on the natural ecosystem. The urbanized index(UI) of all over the investigated areas was about 12.4%. Useful resources plants were composed edible source(42.1%), medicinal source(33.8%), pasture source(28.8%), ornamental source(11.8%), timber source(5.2%), fiber source(1.4%), industrial source(1.1%). Vascular plants of unknown use were 140 taxa(21.4%)

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