Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

[Book Review:] Yi Jin-sang’s “the Theory of Sim jeuk ri (Mind is the Principle),” in Complete Works of Hanju.

( Choi Young-jin ) 성균관대학교 유교문화연구소 2014 儒敎文化硏究(中文版) Vol.0 No.21

충청지역 판소리 문화 유적 현황 연구

최혜진(Choi, Hye Jin) 한국구비문학회 2018 口碑文學硏究 Vol.0 No.48

본 논의는 충청지역의 판소리와 관련된 인물들과 지역을 살피고, 발굴해야 할 문화유적과 기초자료의 중요성을 강조한 글이다. 특히 충남지역의 판소리적 위상을 살피면서 현장 답사를 시행하였고, 판소리 지형도를 살펴, 시급히 조사 연구해야 할 명창과 유적에 대해 논하였다. 먼저 조선창극사에 나타난 충청지역의 명창 23명과 후대의 주요 명창, 명고 등 35명의 생애과 정보, 의의를 정리하고 충남지역이 판소리 발생기와 일제강점기에 매우 중요한 역할을 하였음을 밝혔다. 다음으로는 현지 답사를 중심으로 홍성, 서산, 서천, 공주, 논산 지역의 명창 관련 정보와 문화유적 등을 조사하여 보고하였다. 홍성에서는 판소리의 시조 최선달의 가계와 묘역이 밝혀졌으며, 김창룡의 주거지 등이 남아있음을 알 수 있었다. 한성준 관련 여러 유적도 제대로 보전되어야 할 필요성이 있다. 또한 동편제의 다른 맥을 만든 정춘풍에 대한 지역조사가 시급하다. 서산에서는 심정순 일가와 연관되어 낙원식당 등이 중요한 유적지가 될 수 있으며, 이를 활용할 필요가 있다고 보았다. 고수관 등의 유적지를 조사하였고 방만춘 일가 조사의 필요성을 제기하였다. 서천에서는 이동백과 김창룡 관련 유적지가 발굴되고 보호되어야 한다. 공주에서는 박동진 전수관의 현황을 둘러보았고, 황호통이 중요한 명창으로 활약했음을 밝혔다. 이와 함께 황해천, 김석창, 박상도, 이동백과 김창룡, 고수관 등이 활약했고 거주했던 지역으로 공주의 판소리 문화를 함께 연구할 필요성을 논했다. 논산은 중고제의 시조라 불리는 김성옥이 태어난 곳이고, 그의 생가터가 추정 발굴되고 있다. 이에 대한 관심이 필요할 것이다. 초기 판소리는 충남지역을 중심으로 위로는 경기권, 아래로는 전라권으로 뻗어나가 판소리 문화를 확산 개발시킨 중요한 역사적 사명을 감당하였다. 현재적 의미에서 중고제는 충청지역을 중심으로 발굴, 보전되어야 할 필요가 절실하며 이를 위한 기초연구가 시급히 시행되어야 한다. 흩어져 있는 중고제 관련 명창과 유적지의 정보를 최대한 모아서 충청도 판소리의 위상을 세우는 작업을 하는 일이 필요하다. 중고제 소리를 제대로 전승할 수 있는 실력있는 명창을 보호하고 육성할 필요도 있다. This article emphasizes the importance of cultural heritages that should be developed upon reviewing persons related to Joonggoje Pansori in the Choongchung area and its basic data. It reviews, in particular, the position of Pansori in South Choongchung by dividing the territory of Pansori, and discussing the master singers and heritages that should be prioritized. First, it summarizes the information about 23 master singers in Choongchung, the history of Chosun traditional opera, and 35 masters in the late period, including their lifetime achievements and meanings, and reveals that they played a crucial role in the development period of Pansori in South Choongchung, and in the Japanese colonial period. Next, it is reported that the information and cultural heritages relate to the master singers in Hongsung, Seosan, Seochun, Gongju, and Nonsan, focusing on field visits. In Hongsung, the family history and the boundaries of Sun Dal Choi’s grave, the founder of Pansori, were found, and Chang Ryong Kim’s residence was revealed. It is necessary to conserve a variety of heritages related to Sung Jun Han. In addition, it is urgent tolocally investigate Choonpoong Jung who developed the different roots of Dongpyunje. In Seosan, the Nakwon restaurant, and so on, relate to the family of Jung Soon Shim, and could be an important historical place, which should be utilized. A historical place, such as Gosoogwan, was investigated, suggesting the needs to investigate the family of Man Choon Bang. In Seochun, the historical places related to Dong Baik Lee and Chang Ryong Kim should be developed and conserved. In Gongju, the statuses of Dong Jin Park and Soo Gwan Go were reviewed, and it was revealed that Ho Tong Hwang had been performed as an important master singer. Together with this, further studies are required on Pasori culture in Gongju, where Hae Chon Hwang, Suk Chang Kim, Sang Do Park, Dong Baik Lee, Chang Ryong Kim, and Soo Gwan Go were actively performed and lived. Nonsan is where Sung Ok Kim, a founder of Joonggoje, was born, and his place of residence is under development. Hence, attention in this is required. Joonggoje Pansori took part in the important historical mission to expand and develop Pansori culture up to the Gyunggi area, to the North, and Cholla area, to the South, in the center of South Choongchung. It is urgently required that Joonggoje Pansori is developed and conserved in the center of the Choongchung area, which requires basic research on this. It is necessary to establish the position of Choongchung Pansori by collecting the information of master singers and historical places related to Joonggoje, which are scattered around. Also, it is necessary to protect and develop the master singers who are well placed to succeed Joonggoje Pansori.

Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr

Ehlers - Danlos 증후군

주용진,최성문,최영자,성호석 ( Yoing Jin Joo,Sung Moon Choi,Young Ja Choi,Ho Suk Sung ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Ehlers-Danlos syndrome Yoing Jin Joo, M.D., Sung Moon Choi, M.D., Young Ja Choi*, M.D., Ho Suk Sung, M.D. Department of Dermatology, Clooego of Medicine, Inje University, Pusan, Korea We presented a case of Ehlers-Danlos syndrome in a 25-year old female, who had hyperextensibility of skin, skin fragility, cigarette paper-like atrophic scar, joint hyperextensibility and skeletal abnormality of scoliosis since birth. There was no family history. Microscopic and electron microscopic examinations of the skin biopsy specimens from normal appearing skin on the forearm and the site of subcutaneous nodule was compatible with finding of Ehlers-Danlos syndrome. Although the manifestations of this case including marked extensibility of skin, joint, skin fraility and atrophic scar might be in accord with type Ⅰ(gravis) Ehlers-Danlos syndrome, skeletal abnormality of scoliosis did not correspond to this type, but to type Ⅳ(hydroxylysine deficient) Ehlers-Danlos syndrome. We consider this case as a type Ⅳ Ehlers-Danlos syndrome.

국내 조혈모세포이식 환자에서 Human Cytomegalovirus gB 유전형의 분포와 질환과의 연관성 : 예비 보고

최수미,김진희,이동건,박선희,최정현,유진홍,박철민,이종욱,민우성,황응수,신완식,김춘추 대한감염학회 2007 감염과 화학요법 Vol.39 No.2

목적 : 사람 거대세포바이러스(Human cytomegalovirus, 이하 HCMV) glycoprotein B (gB)는 UL55 유전자에 의해 부호화되는 당단백으로, UL55 유전자의 염기서열변화에 따라 4가지 유전형으로 나뉜다. 본 연구에서는 국내 조혈모세포이식 환자들에서 HCMV gB 유전형의 분포와 그 특성을 알아보고, gB 유전형에 따라 특정 HCMV 질환 발생과 연관이 있는지 그 임상적 의미를 분석해 보고자 하였다. 재료 및 방법 : 동종 조혈모세포이식 환자 52명의 혈액검체 94개에 대해, Chou 등이 제시한 방법에 따라, 먼저 UL55 유전자 부위를 nested PCR로 증폭한 후, RsaI과HinfI으로 Restriction fragment length polymorphism(RFLP) 분석을 시행하였다. 결과 : gB type 1은 73.1% (38/52), gB type 2는 13.5%(7/52), gB type 3는 1.9% (1/52), gB type 1과 type 2에 의한 혼합감염은 9.6% (5/52)로 나타났다. gB type 4는 관찰되지 않았다. 1명(1.9%)에서 gB 유전형을 결정할 수 없었는데, RFLP 패턴으로 보아 Trincado 등이 제시한 gB type 7에 해당하는 것으로 생각되었고, 이 새로운 아형에 대해서는 현재 염기서열 분석 중이다. 52명 중 5명(9.6%)에서 HCMV 질환이 발생하였고, 3명에서 HCMV 폐렴, 1명에서 망막염과 위장관염, 나머지 1명에서 망막염이 발생하였다. 5명 중 HCMV 질환과 관련하여 사망한 예는 없었고, 감염된 HCMV는 모두 gB type 1이었다. HCMV gB유전형과 HCMV 질환 발생 사이에 유의한 연관성은 없었고, 단일 주에 의한 감염과 혼합감염에 따른 HCMV 질환발생 사이에도 유의한 연관성은 관찰되지 않았다. 자료 분석 중 gB type 2에 감염되어 있던 환자에서 gB type 1에 재감염 되면서 발열, 간효소 수치 상승 및 pp65 HCMV 항원혈증이 나타난 예가 있었다. 결론 : 연구결과 gB type 1이 아주 우세하고, gB type 4는 검출되지 않았으며, 혼합감염의 빈도가 비교적 낮은 분포를 보였다. 이는 외국의 보고와는 다른 국내 조혈모세포이식 환자에서의 독톡한 감염 양상으로 추정된다. 본 연구에서 HCMV gB 유전형과 질환 발생과의 연관성을 밝힐 수는 없었으나, HCMV 유전형에 대한 연구는 바이러스 감염의 발병기전이나 전파 경로 및 양식과 같은 역학적 연구에 중요한 자료가 될 것이다. 현재 더 많은 수의 조혈모세포이식 환자를 대상으로 연구가 진행 중에 있으며, 앞으로 다른 질환군의 환자나 건강한 잠복 감염자에서의 추가 연구가 필요할 것으로 생각된다. Background : Human cytomegalovirus (HCMV) glycoprotein B (gB) is the major envelope glycoprotein, encoded by the UL55 gene. Based on sequence variation in the UL55 gene, HCMV can be classified into four gB genotypes. Previous studies have suggested an association between HCMV gB genotypes and clinical outcome in the immunocompromised hosts. The goal of this study was to determine the distribution of HCMV gB genotypes and the effect of gB genotype in the developement of HCMV diseases in hematopoietic stem cell transplant (HSCT) recipients in Korea. Materials and Methods : DNA was extracted from 94 blood specimen of 52 allogeneic HSCT recipients with HCMV infection. HCMV gB genotype was determined using polymerase chain reaction to amplify a region of UL55, followed by restriction fragment length polymorphism (RFLP) analysis based on RsaI and HinfI digestion. Results : The distribution of gB types were as follows: gB1, 73.1% (38/52) of patients; gB2, 13.5% (7/52); gB3, 1.9% (1/52) and mixed infection (gB1 and gB2), 9.6% (5/52). While gB4 was not detected, a new genotype (described as gB7 by Trincado et al, 2000) was identified on the basis of their RFLP pattern. During average 708 days’ follow up period, HCMV diseases developed in 5 patients. All of them had gB1 genotype. There was no statistically significant association between the incidence of HCMV diseases and the gB genotypes. Re-infection with gB1 strain was detected in one patient who had been previously infected with gB2. This episode was associated with fever, elevated liver enzyme and positive antigenemia. Conclusion : HCMV gB1 was the dominant genotype and no gB4 was detected in allogeneic HSCT recipients in Korea, which is an unique pattern compared with the previous reports. Although we can not find significant association between the HCMV diseases and the gB genotypes, genotyping of HCMV will serve in the study of pathogenesis and transmission of this virus in transplant patients. Further study is underway with large study population.

Exception Handling in Defense Industry Logistics Process Using Process Repository

Choi,Doug Won,Shin,Jin Gyu 韓國防衛産業學會 2010 韓國防衛産業學會誌 Vol.17 No.1

일상적인 상황에서 방위산업에 관련된 물류관리의 중요성은 일반 산업에 비하여 크지 않으나, 예외적인 상황이 발생했을 때에는 신속한 해결책을 찾아내어 문제를 해결하지 않으면 국방에 있어서 전략적 공백으로 이어질 수 있다. 그러므로 예외상황에 대한 신속한 대비책을 구축하는 것이 필요하며 본 논문에서는 업무절차관리(BPMS) 시스템을 도입하여 물류 프로세스의 지속적인 개혁 및 개선을 유도한다. 이를 위하여 프로세스 저장소의 사용을 제안하며, 예외발생시 신속한 처리방안을 검색하기 위한 검색트리의 사용방법론을 제시한다. 획일적인 업무처리가 강조되는 군사작전 및 방위산업의 환경 하에서 예외상황의 처리를 위한 효율적 장치를 마련하는 것은 방산운용의 측면에서 매우 중요하다. 본 논문에서는 특히 지식경영의 관점에서 예외처리 시 생성되는 새로운 프로세스 지식을 프로세스 저장소(process repository)에 저장하고, 예외발생시 이 문제의 해결에 적합한 프로세스를 저장소에서 신속하게 검색하는 데 활용할 수 있는 검색트리의 생성과 활용에 대한 방법론을 연구하였다. 검색트리의 생성과 활용을 위하여 ‘상황변수’와 ‘의사결정변수’의 개념을 새로 도입함으로써 문제의 상황을 체계적으로 기술하고 저장 및 검색하는 방편을 제시하였으며, 예외상황의 인식능력(context awareness)을 제고시켰다. 정보의 엔트로피를 기초로 한 C5.0 알고리즘을 적용하여 정보이득을 최대화하는 검색트리를 생성하는 방법을 제시하였다.

Reliability Considerations of Hybrid Power Systems for Critical Facilities

Choi, Se Jin · Ham, Young Hwan · Kim, Myung Chin 충북대학교 컴퓨터정보통신연구소 2008 컴퓨터정보통신연구 Vol.27 No.2

본문참고

진주지역 제조기업의 산업재해 실태분석과 대책방안

최진현,강호욱 경상대학교 경남문화연구소 1983 慶南文化硏究 Vol.6 No.1

The process of manufacturing enterprise takes the potential injury agents (moving equipment, machines and tools, hazards, etc). The industrial accidents due to these occasion happen unexpectedly. Safety management, in the manufacturing enterprise, are a moral action for worker, and improve the production efficiency and decrease the accident cost. In this study, industrial accidents are analyzed on the actual condition for 339 manufacturing enterprise, and select the 9 sampling enterprise. We simulate the relationship between accident cost and safety management cost. So, We suggest the safety management counterplan. The actual condition of the industrial accidents were analyzed by I.L.O. statistical analysis equations, which are expressed by (1) and (2). FR=N/H×10^6 …(1) SR=S/H×10³ …(2) Logical development of the alternatives for safety management counterplan based on the structure of industrial accident. We derived characteritics of the industrial accidents, and suggested a few counterplan of industrial accidents for manafacturing enterprises in Jin-Ju area.

How Young Consumers Perceive Firms’ Environment, Social, and Governance (ESG)

Choi,Woo Jin,Yoon,Ho-Jung 한국상품학회 2021 商品學硏究 Vol.39 No.6

Demand for ESG is surging. However, not many are known regarding how consumers perceive firms’ ESG. To fill this gap in the literature, the current research seeks to explore how firms’ ESG is perceived by the young generation as well as to explore how the young generation perceive South Korean firms from the ethical perspective. For this purpose, we surveyed ninety-two respondents (35.8% male; Mage=23.5) and they completed the survey. In the survey, respondents indicated their perception regarding South Korean firms’ ethical business management and South Korean firms’ ESG management. Most respondents said ESG would be important (88%) and helpful to achieve competitive advantage (81.5%). However, interestingly, almost of respondents (46.7%) said that ESG would be helpful to maximize productivity. Rather, 92.4% of respondents perceived that ESG would be helpful to enhance firms’ images. In addition, they also perceived that the importance of ESG would become great in the future (85.9%) enough to make a significantly positive impact on firms’ productivity (72.8%). In addition to these findings about ESG, we further investigated how this young generation perceived the ethical business climate among South Korean companies. The results show that only 17.3% of respondents said South Korean business is ethical and 70.6% thought ethical problems would be a problem in the future. While the investigation for South Korean consumers’ perception of ESG and ethical business is not many, our work makes a significant theoretical contribution by providing empirical evidence on this issue. Our results are also managerially meaningful by being a reference to South Korean companies as well as global companies who are interested in the Korean market.