Production and Characterization of Enzymes Involved in Chitin Catabolic Cascade from a Bacterial Strain Isolated from Soil

( Ji Hyun Lee ),( Jeong In Lee ),( So Yeon Park ),( Yoo Kyeong Jang ),( You Bin Lim ),( Hye Yoon Lee ),( Young Min Jung ),( Hae Chang Lee ),( Jong Hwa Lee ),( Shaheen Amna ),( Jae Kweon Park ) 한국키틴키토산학회 2019 한국키틴키토산학회지 Vol.24 No.1

The present study was aimed to isolate the bacteria producing chitinolytic enzymes including chitin deacetylase (CDA) from soil origin. About 24 bacteria were screened for their chitinolytic enzymes producing ability on the basis of colloidal chitin. Amongst one of the most potent isolates designated to JI-02 was further selected as the most prominent chitinolytic and CDAlike enzyme (CDA-X) producer based on the ability of deacetylation of an artificial substrate pNP-acetamilide. The maximum production of CDA-X in crude enzyme from JI-02 was observed in the presence of 1% colloidal chitin at 37°C and pH 6.4 after 120 h of incubation. Potent enzyme activity of CDA-X was determined toward pNP-acetamilide, demonstrating that optimal pH, temperature and ionic strength of the CDA-X were assessed to be 7.0, 52°C and 125 mM, respectively. Unexpectedly, Nacetylglucosamine (GlcNAc) was not fully converted by the action of CDA-X to glucosamine (GlcN) under the established conditions. Further study on enzyme activity toward chitin-oligosaccharides consisting of multi-N-acetylglucosamine (GlcNAc)n, n=2-5 may be necessary to elucidate the mode of action which requires the minimum size of (GlcNAc)n. However, our data suggest that CDA-X can convert chitin to chitosan with the maximum yield of approximately 0.08g/L during the fermentation of the strain in the presence of chitin. Furthermore, we found that crude enzyme showed the significant chitosanolytic activity. Taken together, our data suggest that the strain JI-02 may be a potential candidate as a particular strain for better understating of chitin catabolic pathway.

한국예술가곡 효시에 관한 연구

이지연 ( Ji-yeon Lee ),공정배 ( Jeong-bae Kong ),김용범 ( Yong-beom Kim ) 한국사상문화학회 2016 韓國思想과 文化 Vol.85 No.-

본 연구는 독일어권에서 비롯된 예술가곡 리트(Lied)의 한국적 전이과정을 통해 한국예술가곡의 효시를 규명하고자하는 목적이다. 예술가곡은 시문학과 음악의 합일된 예술장르로서 독일과 오스트리아를 포함한 독일어권에서 발달된 가곡으로, 시인의 시에 작곡가가 선율을 붙이고 이를 성악가가 부르는 음악 양식이다. 그 효시를 슈베르트의 `실 잣는 그레첸(Gretchen am Spinnrade)`로 비정하고 있다. 작곡가의 임무란 좋은 시를 널리 알리는 것이었기 때문에 음악은 시에 종속되어 있었다. 그러나 예술가곡은 상호매체성에 의한 문화융합결과물로서 시와 음악이 서로가 각기 독립된 예술이었던 상태로부터 벗어나 완전히 하나로 융합되면서 또 하나의 새로운 양식으로 탄생한다. 슈베르트 이후 가곡의 작곡자는 더 이상 시의 언어 음율을 선율적으로 고양시키는 낭음자가 아니라 가사에서 말로 표현될 수 없는 영역을 음악적으로 표출하는 시의 자유로운 해석자의 역할을 하게 된다. 음악을 단순히 시를 쉽게 이해하는 보조수단에 국한시킨 이전의 가곡미학을 극복하고자 하는 새로운 경향들이 나타나면서 이전까지 작법인 유절가곡이 아닌 통절 작곡 형식이 새롭게 떠오르기 시작하였다. 이렇게 완성된 예술가곡은 1835년경 이후부터 독일을 벗어나 다른 나라로도 전파되어, 각 나라의 음악적 특징에 맞는 독자성을 지니며 민족음악으로 자리잡는다. 그리하여 그 갈래의 하나가 한국에 이르게 된다. 독일 예술가곡의 개념과 정의 및 조건에 맞는 한국 최초의 예술가곡이 어떤 작품인가는 학자들의 견해에 따라 논란이 존재 한다. 그러나 이 관점은 예술가곡을 시와 음악의 등가의 결합을 전제로 보았을 때 우리 시문학사적 위상과 일치하는 시기이다. 한국 시문학사는 1930년대에 이르러서 성숙함이 묻어나면서 시인들은 창작 행위에 대한 확고한 자신의 색깔을 드러내기 시작했다. 이 시기에 전문적인 시인의 등장이 활발해지는데 이때 정지용을 대표적으로 꼽을 수 있다. 1932년 7월 동방평론 4호에 발표한 정지용의 시 `고향`에 작곡가 채동선이 곡을 붙였고, 채선엽이 독창을 맡았으며 반주는 김애식이 맡았다. 작곡가 채동선은 독일에서 정통으로 작곡을 공부하고 귀국하여 <고향>을 작곡하였고 시기적으로는 물론 예술가곡의 완성의 필요충분조건은 모두 갖춘 한국 최초의 예술가곡 그 효시로 비정할 수 있다. This study aims to clarify the beginning of the Korean art song by reviewing how the German art song "lied" was traditionally transferred and spread in Korea. As a genre of art that unites poetry and music, the art song was originally developed in German-speaking countries including Germany and Austria; the then composers put a melody on poems so that singers(vocalists) could sing the songs. Gretchen am Spinnrade by Franz Peter Schubert is considered the beginning of such a style of music. Since promulgating good poems was one of the composer`s missions then, music was viewed as subordinate to poems. As an outcome of cultural convergence through intermediality, however, the two independent forms of art, poetry and music, came to converge into a completely single genre, heralding a birth of a new music style. Since Shubert, composers were no longer a mere reciter exalting poems by putting melodies on rhythmical poetic language; instead, they served as a free interpreter of poems to express the sentiment beyond the lyrics musically. In an attempt to overcome the stereotype that regarded music simply as an aid to better understand poems, a new trend such as through-composed songs, away from traditional strophic songs, emerged. Those German art songs were widely disseminated beyond Germany since 1835, and took root in many areas as folk music combined with each country`s traditional musical hallmarks. Korea was no exception. With Korean art songs originated from Germany, scholars in the field have long disputed over which one is the first Korean art song that complied with the concept, definition, and conditions of the German lied. When we consider the art song as a fair combination of poetry and music, a hint can be found from the history of Korean poetry. Korean poetry began to mature in the 1930s with poets clearly expressing their own signature colors in their creations. Professional poets actively broke onto the art scene, and Ji-yong Jeong was one of them. Mr. Jeong released a poem titled "Home (Gohyang)" on No. 4 Dongbang Critique in July, 1932, and Dong-sun Chae, a composer, put a melody on it; later it was sung by Sun-yub Chae with the piano accompaniment by Ae-sik Kim. After studying composition officially in Germany, Dong-sun Chae returned to Korea and composed the song, meeting all of necessary and sufficient conditions of the German lied. In this sense, "Home (Gohyang)" can be regarded as the first Korean art song.

임부의 연령에 따른 보완대체요법에 관한 인식 태도 경험 비교 연구

김연진,김지연,노경민,신혜리,유예지,이지민,장희조,정아인,최소정,강숙정,이향연,민혜영 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: This study aims to test if the age of pregnant women affects the perception, experience, and attitude towards complementary alternative therapy (CAT), by investigating the perception, experience, and attitude of pregnant women under the age of 35, and above 35 (Advanced Maternal Age). The study will 1) find the general characteristics of pregnant women under and over the age of 35 2) compare the perception, behavior, and experience of pregnant women under and over the age of 35, and 3) find the comparison of the perception, behavior, and experience according to the general characteristics of pregnant women under and over the age of 35. Method: The subjects of this study are composed of 44 women under the age of 35 and 44 women above the age of 35, totaling 88 pregnant women visiting midwiferies, obstetrics and gynecology, and baby fairs in Seoul and Gyeong-Gi area. Data for this study was collected from November 28, 2015 to January 6, 2016 for 40 days. The collected data analyzed mean, standard deviation, t test, ANOVA, and chi-square by using SPSS 23.0. Result Results show that there is a significant difference in the perception, attitude, and experience of the two-sample groups in the use of CAT. The question of “what is most necessary when applying CAT in a hospital clinical environment” for the perception criteria came out with the results of t=9.980, p<.05. For the attitude, results showed that the answers of pregnant women under the age of 35 displayed a more positive attitude towards CAT. (t=2.421, p<.05) Lastly, in the experience section, pregnant women under the age of 35 showed more CAT use than the other sample group. Conclusion Results have shown that there is a difference in the perception, behavior, and experience between the two sample groups. Therefore further research on the efficacy and the adverse effects of CAT needs to be done.

역추적 에이전트를 이용한 역추적 시스템 설계 및 구현

정종민,이지율,이구연 江原大學校 産業技術硏究所 2002 産業技術硏究 Vol.22 No.B

It is very important to detect and remove original sources of DOS (Denial of Service) attacks or connection oriented/connectionless attacks. In this paper, we implement a traceback system that does not require the reaction of routers and administrators and does not need log data. We bring in a traceback server and traceback agents in each network and use sniffing and spoofing schemes. Finally, the traceback server detects attacking hosts using information transmitted from traceback agents.

신경회로망을 이용한 이면비드 폭 예측에 관한 연구

정재원,김일수,박창언,김학형,서주환,김인주,심지연 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

The development of robotic welding process is a very complex assignment because the system is affected by a number of process parameters which are very difficult to determine or predict in practice. The full automation welding has not yet been achieved partly because the mathematical model for the process parameters of a given welding task is not fully understood and quantified. Several mathematical models to control welding quality, Productivity, micro structure and weld properties in arc welding processed have been studied. However, it is not an easy task to apply them to the various practical situations because the relationship between the process parameters and the bead geometry is non-linear and also they are usually dependent on specific experimental result. Practically, it is difficult, but important to know how to establish a mathematical model that can predict the result of the actual welding process and how to select the optimum welding condition under a certain constraint. In this paper, an attempt has been made to develop an neural network model to predict the back-bead width as a function of key process parameters in the GMA (Gas Metal Arc) welding and to compare the developed models with the experimental results

폐지 소각 공정의 전과정평가

정수정,류지연,허탁 한국전과정평가학회 2004 한국전과정평가학회지 Vol.5 No.1

본 연구에서는 전과정평가(Life Cycle Assessment, LCA)기법을 이용하여 폐지 소각 공정에 대한 전과정영향평가를 수행하였다. 폐지 1kg을 소각할 경우, 전력 0.089kwh, 용수 0.86kg, 각종 화학약품 0.0086kg이 투입되고 대기배출물로 이산화탄소는 1.13kg, NOx는 0.0015kg 발생되고 바닥재와 비산재는 각각 0.1kg, 0.013kg 발생하며 폐열을 이용한 스팀 에너지 3.42MJ이 회수된다. 소각 공정 자체에 의한 이산화탄소 배출은 1.13kg/f.u.이며 에너지 회수로 인하여 0.32kg/f.u.가 환경 이득이 되어 최종적으로 0.82kg /f.u.의 이산화탄소가 발생한다. 전과정 영향평가 결과, 지구온난화가 77.7%, 자원고갈 6.3%, 산성화, 부영양화, 생태독성 순으로 조사되었다. 이산화탄소의 주원인인 탄소함량을 줄이면 환경영향이 줄어들 수 있으나 일반적인 종이의 탄소함량은 일정하므로 이는 해결방안으로 적절치 못하다. 그렇다면 폐열 에너지 회수율을 높이는 것이 전체 환경부하 저감에 도움이 될 것이다. 회수율을 높이는 방안으로는 수분함량을 줄이는 것, 즉 폐지와 함께 섞여 들어오는 음식물 쓰레기의 양을 줄이면 에너지 회수율을 좀 더 증가시켜 환경부하를 저감하는 하나의 방안이 될 것이다. 또한 보다 근본적인 해결방안으로는 되도록이면 현재 56% 정도인 폐지의 재활용률을 높여 폐지소각으로 인한 환경부하 발생을 감소시켜야 할 것이다. This study is about practicing LCA on the incinerating waste paper. One kilogram of waste paper needs a 0.089kwh of electricity, 0.86kg of water and 0.0086kg of various chemicals while burned. As a result, 1.13kg of CO2, 0.0015kg of NOx are eliminated as the outcome to the air. Also, 0.1kg of ashs and 0.013kg of arsenic acids are produced. Steam caused by using waste heat is recovered by 3.42MJ. The exhaust of CO2 in the stage of incinerating itself is 1.13kg/f.u. but it gets 0.32kg/f.u. from the energy recovery, so, the total counts 0.82kg/f.u. The order of impact categories from the LCA result is global warming(77.7%), abiotic resource depletion(6.3%) and acidification, eutrophication, ecotoxicity. Even though it might somewhat reduce the bad effect on ecology to cut back the amount of carbon, which is the main ingredient in CO2, however, given that the amount of carbon in paper remains unchanged, that is not the proper plan. Now we can suppose that promote energy recovery would help minimizing the overall burden on the environment. One solution to increase the rate of energy recovery is reducing the content of water in food waste came along with waste paper. And this results in lessening the burden on the environment. Promoting the recycling rate of waste paper, which accounts 56% now, might be the basic solution to reduce the burden on ecology generated from the incineration of paper.

Horseshoe Appliance를 이용한 Ⅲ급 부정교합의 교정적 접근

한지혜,김재곤,,백병주,양연미,서정아 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.4

성장기 아동의 Ⅲ급 부정교합의 치료에는 많은 악기능 정형장치가 사용되어 왔으며 다양한 형태로 변화하여 발전되어 왔다. Horseshoe appliance는 Schwarz에 의해 소개된 장치로서 elastic의 견인력으로 장치의 free sliding 효과를 통해, 하악와와 과두의 관계를 재정립시켜, 악골 주위의 연조직이나 muscle matrix와 조화되는 새로운 위치에 하악골을 repositioning시켜 악골관계를 개선시킬 수 있다. 또한 하악의 후하방 회전 효과를 극소화시켜 전안면고경의 증가를 방지할 수 있고, 구외 장치에 비해 환자의 협조도가 좋다. 저자들은 전치부 반대교합을 주소로 전북대학교 치과병원 소아치과에 내원한 Ⅲ급 부정교합 환자에게 Horseshoe appliance를 사용한 결과 하악골에 대한 상대적인 상악골의 전방 성장과 교합평면의 반시계방향 회전, 상악 전치의 순측 경사와 하악 전치의 설측 경사로. 3개월 내에 전치부 반대교합이 해소되는 효과를 얻었다. The Horseshoe appliance was introduced by Dr. Schwarz, and it is used to correct sagittal relationships by elastic force in class Ⅲmalocclusion.It minimizes the increment of lower anterior facial height and allows the mandible to be repositioned harmoniously with the soft tissue and muscle matrix of the jaw. It has the advantages of better patient cooperation, easier construction, and more effective modification. In the patients who were treated with Horseshoe appliance, forward growth of maxilla and counterclockwise rotation of occlusal plane with labioversion of maxillary incisors and linguoversion of mandibular incisors were obtained Minimum downward and backward rotation of mandible was accepted, so increasing of lower anterior facial height was minimized.

電氣集塵機 效率向上을 위한 放電極 印加電壓 特性에 關한 硏究

李廷株,金鍾浩,都硏知 龍仁大學校 1996 용인대학교 논문집 Vol.12 No.-

The purpose of this study was to determine the optimum collection efficiency for various applied voltage of Electrostatic Precipitator. The experiment was performed between 15kV and 30kV. Collection efficiency was measured by Anderson Sampler(cascade impactor). The results of this experiment were as follows ; (1) In order to determine the ESP's optimum operating condition, we measured an electric property of discharging electrode. (2) Optimum applied voltage and overall collection efficiency were 25kV and 98.4%, respectively. (3) When applied voltage value was higher than 25kV, the collection efficiency shows a tendency to decline. We estimated that this result was due to ionic winds generated by electric field.

항결핵 6개월 단기요법의 성과 고찰

서지원,정연채,김남재,홍석철,김주옥,김선영,노흥규 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

To evaluate the effect of 6-month short term antituberculosis chemotherapy with INH, Rifampin, Ethambutol, and Pyrazinamide(2HREZ/4HRE) in the patients with pulmonary and/or extrapulmonary tuberculosis, the authors prescribed 2HREZ/4HRE regimen in 79 tuberculosis patients for 6 months with measuring the sputum staining for AFB, chest X-ray findings, recurrence rates and possible side reactions of the treatment. The result were as follow; 1. Pulmonary Tuberculosis 1) Among the 56 pulmonary tuberculosis patients who had taken 2HREZ/4HRE regimen. 32 patients showed initial positivity in sputum AFB smear stain(57.2%). Negative conversion occurred usually within 2 months after initiation of chemotherapy and the mean period of negative conversion was 1.4 months. 2) Among the 56 pulmonary tuberculosis patients, chest X-ray finding changed in 41 patients (37.21%). From these 41 patients 39 patients showed continuous improvements in chest X-ray finding, though 3 patients showed initial aggravation in spite of continuous medication. The remainder 2 cases aggravated due to the failure of treatment. 3) Treatment failure occurred in 2 patients (3.57%) during the chemotherapy among 56 patients of pulmonary tuberculosis and they were infected with secondary drug-resistant strains of M. tuberculosis. 2. Extrapulmonary tuberculosis. Among 8 patients with tuberculous pleurisy and 15 patients with superficial tuberculous lymphadenitis, there were no evidence of treatment failure after completion of antituberculosis chemotherapy for 6 months with 2HREZ/4HRE regimen. 3. Follow-up study was performed from 6 months to 50 months after completion of antituberculosis chemotherapy and the relapse was not noted in both pulmonary and extrapulmonary tuberculosis patients group during this period. 4. Serum AST/ALT elevated in 9 patients(11.4%) during the treatment and this occurred usually within 3 months after the initiation of antituberculosis chemotherapy. However treatment interruption occurred in 2 patients (2.5%) due to the development of hepatitisone due to drug-induced hapatitis and the other due to type B viral hepatitis. In conclusion, we could find this 2HREZ/4HRE 6-months short-term antituberculosis regimen is effective and could be recommanded as a promising regimen for the treatment of tuberculosis.

학령기 아동의 신체상과 학교생활 적응

한지영;윤희옥;정경순 김천과학대학 2005 김천과학대학 논문집 Vol.31 No.-

The purpose of this study is to investigate the relation between body image and school adjustment among elementary school students. The subjects of this study were 120 students who were currently enrolled in 5th grade elementary school in Andong-si. The data were collected by self - reported questionnaire and collected data were analyzed SPSS WIN 12.0. The results of this study were as followings. First. The mean score of body image was 29.70 (SD 5.27). Second. The mean score of school adjustment was 84.29(SD 12.24). Third. The relation between body image and school adjustment showed a positive correlation, which was significant statistically( r = .36, p<.01). these results demonstrated that there is a strong interrelationship between body image and school adjustment among elementary school students ; the higher body image is, the higher school adjustment, therefore, it is suggested that positive body image can help to do successful school adjustment.