홍삼 엑기스를 함유한 분말주의 제조 및 평가

이사원,최한곤,박정일,김종국 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

To develop a dry alcohol containing red ginseng extract, dry alcohols composed of ethanol, water, dextrin and sodium lauryl sulfate were prepared using spray dryer, and their ethanol contents and encapsulation efficiencies were deter-mined. An optimal dry alcohol containing red ginseng extract was chosen and the feeling for its oral administration was eval-uated. Dextrin at dextrin/water weight ratios below 1.6/1 and ethanol at ethanol/water weight ratios below 1/1 remarkably increased both the ethanol contents and encapsulation efficiencies of dry alcohols. However, dextrin at dextrin/ water weight ratios above 1.6/1 and ethanol at ethanol/water weight ratio above 1/1 slightly decreased the both parameters. It might be due to the low solubility of dextrin in ethanol and limited diffusion coefficient of ethanol to the dextrin shell. Furthermore, 0.5%(w/w) sodium lauryl sulfate gave the maximum ethanol content of dry alcohol. The more increased amounts of red ginseng extract were added, the more increased amounts of ginsenoside Rb1 but the more decreased amounts of ethanol were encap-sulated in dry alcohols. A dry alcohol containing red ginseng extract was prepared with dextrin/ethanol/water(1/1/1, w/w/w) mixed solution, in which 0.5%(w/w) sodium lauryl sulfate and 20%(w/w) red ginseng extract were dissolved. It contained the ethanol contents of 31.17±1.33%(w/w) and ginsengoside Rb1 of 243.0±7.0㎍/g. It gave the moderate taste of red ginseng extract at its oral administration with or without water. Thus, the dry alcohol containing red ginseng extract can be further developed as a more convenient dosage form for red ginseng extract.

자동차용 실리콘 가속도센서의 개발(Ⅱ)

이종현,신장규,이상룡,천희곤,조찬섭,심준환,류인식,박석홍,허정준,박기열 경북대학교 센서기술연구소 1994 연차보고서 Vol.1994 No.-

자동차의 air-bag 장치에 실용될 수 있는 압저항형 단결정 실리콘 가속도센서 칩을 개발하기 위하여 결정 실리콘 미세구조의 제조방법을 확립하고, 단위공정의 검증을 통하여 일괄공정에 의한 PROTO-TYPE 칩을 만드는 기술을 연구하였다. 단결정 실리콘 미세구조는 선택확산법을 이용하여 정확히 선택된 영역에만 air-gap을 형성하여 미세구조의 측면식각을 방지하는 선택확산법에 의한 실리콘 마이크로머시닝 기술로 제조하였다. 일괄공정을 위한 단위공정확립을 위하여 PROTY-TYPE 8빔 브릿지형 가속도 센서를 제조하였다. 제조된 칩의 가속도에 따른 출력전압은 선형성을 나타내고 있으며, 감도는 약 50 ㎶/V·g로 나타났다. 이 감도는 50G용 가속도센서의 사양을 만족하지 못했다. 이는 공정에 의한 문제라기 보다는 가속도센서의 시뮬레이션에 의해 설계한 구조가 이미 원하는 감도에 못 미친다는 것으로 생각된다. 따라서 2차 공정으로 제조될 가속도센서의 파라미터를 SuperSAP 유한요소 패키지를 이용하여 실리콘 미세구조부의 파라미터에 따른 특성을 시뮬레이션하였다. 설계된 50G용 가속도센서의 mass Pad의 반경 및 빔 길이, 빔 폭, 빔 두께, 그리고 mass의 각 파라미터 값은 700 ㎛, 120 ㎛, 5 ㎛, 1.0 ㎎ 이었다. 반도체 공정기술, 관성질량 제조법 및 선택확산을 이용한 마이크로머시닝을 사용하여 일괄공정으로 8빔 브린지형 가속도센서를 제조하였다. We researched the establishment of the silicon microstructure fabrication technique to develop a piezoresistive type silicon acceleration sensor chip and the technique to make a proto-type chip by the verification of the unit-process. Silicon microstructure is fabricated silicon micro-machining by selective diffusion method. This method prevent a side-etching of microstructure because selective diffused region is only formed an air-gap. We fabricated a proto-type 8-beam bridge-type acceleration sensor to establish the unit-process for the batch-process. The output voltage of the chip represented linearity with acceleration, and the sensitivity was about 50 ㎶/V·g. But this sensitivity dosen't satisfy the requirements of a practical acceleration sensor. The cause of this result is assumed not process problem, but the structure designed by simulation isn't suitable already. Threfore, the characteristics of parameters of the acceleration sensor that will be fabricated by 2nd-process is simulated by SuperSAP finite-element package. The determined parameter values of beam length, beam width, beam thickness, mass, and mass radius are 120 ㎛, 5 ㎛, 1.0 ㎎, and 700 ㎛, respectively. We fabricated 8-beam bridge-type acceleration sensor by batch-process using a semiconductor process technique, proof-mass fabrication method, and micromachinig using selective diffusion.

G-CSF로 체내 증폭된 골수를 이용한 동종 조혈모세포이식

이종욱,김정아,민창기,김희제,엄현석,박수정,서정곤,김동욱,홍영선,민우성,김춘추,김동집 대한조혈모세포이식학회 1998 대한조혈모세포이식학회지 Vol.3 No.2

연구배경: 이식시 주입되는 골수세포수는 생착 속도 및 생존율과 밀접한 관계가 있으나, 임상적으로 환자의 체중이 공여자에 비해 지나치게 많을 때 단위 체중당 환자에게 주입되는 세포의 양이 적어 문제가 될 수 있다. 방법: 저자들은 가톨릭 조혈모세포이식 센터에서 동종 골수이식을 시행한 환자들 중 환자의 체중이 공여자에 비해 10kg이상 많은 경우 골수 채취 전에 공여자에게 3일간 피하로 G-CSF(10ug/kg/day)를 투여하여 골수를 체내(in vivo) 증폭시킨 후 골수이식을 시행한 25예의 환자를 대상으로 고식적인 방법으로 골수이식을 시행한 위험인자가 일치되는 대조군과 비교하여 이식된 세포수, 혈구의 회복속도 및 이식편대 숙주반응(GVHD)의 발생율, 그리고 생존율 등을 비교 분석하였다. 결과: 단기간의 G-CSF 투여로 증폭된 골수의 총 유핵세포수, 단핵구수는 대조군에 비해 3-5배, CD34 + 세포수는 6배 증폭되었으며, 두 군간에 세포의 면역표현형(CD4, CD5, CD7, CD8, CD20)의 차이는 없었다. 중성 백혈구 수가 0.5×10^(9)/L 이상 회복되는 시기는 두 군간의 차이가 없었으나(15.5일 vs 16일; p=0.131), 혈소판 수가 30×10^(9)/L 이상으로 회복되는 시기는 G-CSF 투여군에서 의의있게 단축되었다(20일 vs 26일; p=0.013). 두 군간 급성 및 만성 GVHD의 발생률과 정도의 차이는 없었으며, 이식 후 재발 및 생존율의 차이도 없었다. 결론: 이식전 공여자에게 단기간 G-CSF를 주사하여 생체내에서 골수 조혈모세포를 증폭시킨 후 이식하는 방법은 공여자와 환자의 심각한 체중 차이로 인해 단위체중당 이식되는 조혈모세포양이 부족한 환자들에게 안전하고 유용한 방법일 뿐만 아니라 GVHD의 증가 없이 빠른 혈구회복을 기대할 수 있는 이식기법이라고 생각한다. 향후 증폭된 골수 조혈모세포의 특성 연구 및 이의 효용성을 확인하기 위한 전향적인 연구가 필요하리라고 생각된다. Background: Sufficient stem cell doses are necessary to overcome engraftment failure in allogeneic bone marrow transplantation(BMT). Cell doses harvested may depend on body weight (BW) difference between donor and recipient. In practice, it is important to achieve large number of stem cells from donor who were lower BW than that of recipient. Methods: We have tried to inject G-CSF(10ug/kg/day) subcutaneously for 3 days to the allogeneic donor with lower BW than recipient before harvest to increase BM inoculum. BM was infused into patients without any manipulation on day 5. A total 25 patients were enrolled; 12 AML, 6 ALL, 5 SAA and 2 CML(expansion group). We compared the expansion group with 25 historical control patients, matched for diagnosis and clinical characteristics, who underwent unprimed BMT. Results: Recipient BW is not different between the expansion and control group. However, allogeneic donors in the control group weigh heavier than those in the expansion group. The yield of TNC, MNC, and CD34^(+) cells from G-CSFprimed BM was significantly higher than that from unprimed BM. There was no difference in immunophenotyping analysis(CD4, CD5, CD7, CD8, CD20) between two groups. The median time reaching to absolute neutrophil count more than 0.5×10^(9)/L was not different(15.5 vs 16 days: p=0.131), but time to platelet recovery more than 30×10^(9)/L was significantly shorter for the patients who received G-CSF-primed BM (20 vs 26 days: p=0.013). There was no difference in the incidence of acute and chronic GVHD, relapse rate and overall survival between the two groups with a median follow-up of 13 months. Conclusion: These data suggest that the short-term administration of G-CSF to an allogeneic donor prior to BM harvest seems to be a feasible method to achieve an adequate number of cell doses for patients who weigh higher than donors. Randomized, prospective study is needed to evaluate the efficacy of allogeneic BMT using in vivo expansion of BM by G-CSF.

교합 거상 금관이 어린이 교합 발육에 미치는 영향

신정근,백병주,김재곤,양연미,이선영 大韓小兒齒科學會 2005 大韓小兒齒科學會誌 Vol.32 No.1

혼합 치열기에서 유치의 탈락 및 영구치의 맹출 과정이 순조롭게 이루어지는 것은 중요한 일이다. 맹출 중인 치아는 인접치유무, 유치의 흡수속도, 유치의 조기상실, 만기잔존, 국소병소나 입술, 혀, 저작근 등의 다양한 요인들에 의해 영향 받을 수 있으며, 이러한 요소들 사이의 균형이 깨지게 되면 인접치 간의 관계가 변하고 심각한 부정교합이 초래될 수 있다. 본 증례는 유치열 및 혼합치열을 가진 어린이들에서 상하악 구치부 교합면에 접착된 교합 거상 금속 스플린트(bite raising metal splint)가 치열에 미친 영향에 대해 보여주고 있다. 이들은 스플린트로 인한 유치의 만기잔존으로 영구치 맹출에 방해를 받거나, 전치부 개방교합, 제 1대구치의 저위 맹출 등 혼합 치열기 발달에 큰 영향을 받고 있었다. 치료로써 고속 핸드피스를 이용하여 스플린트를 제거하고 주기적 점검을 통해 교합변화 및 영구치 맹출 양상을 관찰하였다. Children are in mixed dentition during 6 years after 3 years old. this time is very important for sound permanent dentition. There are many factors of influence to tooth eruption stage ; adjacent teeth, tooth resorption, early loss or retention of deciduous tooth, local lesion, lip and tongue, masticatory muscles, ect. These factors should be in balance, if not, relation of adjacent teeth is changed, then severe malocclusion is occurred maybe. These cases revealed influences of resin bonded metal splint on occlusal surface of children's molar to mixed dentition. Splints interfere with falling off of deciduous tooth, tooth eruption, normal occlusion formation, and development of mixed dentition and occlusion. Therefore we removed the metal splint from teeth, follow-up checked occlusion and tooth eruption.

Chlorhexidine과 fluoride 성분의 varnish가 타액 내 mutans streptococci 수에 미치는 효과

서정아,김재곤,백병주,양연미,이용희,김상훈 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.4

본 연구의 목적은 chlorhexidine(Cervitec)과 fluoride(Fluor-protector) 성분을 각각 포함한 varnish를 치아에 적용하였을 때 타액내의 mutans streptococci의 수의 감소에 미치는 효과를 알아보기 위한 것이다. 전북대학교 치과대학생 40명을 대상으로 하여 mutans streptococci 균종 수를 평가하였으며, 이들을 무작위로 네 군으로 나누어 다음과 같이 varnish를 적용하였다; A) varnish를 적용하지 않은 군(n=10), B) chlorhexidine varnish 적용군(n=10), C) fluoride varnish 적용군(n=10), D) chlorhexidine과 fluoride varnish를 모두 적용한 군(n=10), Mutans streptococci 수 측정에는 Orion diagnostica 사의 Dentocult SM을 사용하였다. Varnish를 적용하지 않은 상태에서 1차로 Mutans streptococci 수를 측정한 후 각각의 varnish를 적용하였으며, varnish 적용 12주 후 다시 측정하여 기록하였다. 모든 실험군(group B, C, D)에서 12주 후에 측정한 Mutans streptococci 수가 감소한 개체가 증가한 개체보다 많았으나 통계적으로 유의한 차이는 보이지 않았다(p>0.05). 실험에 사용된 어떠한 varnish도 적용 12주 후에 타액 내 mutans streptococci 수에 통계적으로 유의할 만한 변화를 주지는 못하는 것으로 나타났다(p>0.05). The objective of the present prospective trial was to compare the efficacy of chlorhexidine(Cervitec), Fluoride(Fluorprotector) and combination of chlorhexdine and fluoride varnishes in decreasing the level of salivary mutans streptococci. Forty healthy students of school of dentistry, Chonbuk national university were investigated to evaluate mutans streptococci(MS) counts and randomized into four groups to treat with the experimental varnishes: A) untreated group(n=10), B) chlorhexidine group(n=10), C) fluoride group(n=10), D) chlorhexidine and fluoride group(n=10). Dentocult SM^(�)(Orion Diagnostica) strip method was used for measurement of the level of mutans streptococci in saliva. Stimulated saliva were collected at baseline for mutans streptococci counts evaluation(ms1), 12 weeks later the completion of each varnish treatment, mutans streptococci counts were re-evaluated. In varnish group with chlorhexidine, fluoride and combination of chlorhexidine and fluoride, the level of mutans streptococci was lower after 12 weeks than at baseline, but there were no significant differences in saliva(p>0.05), when compared with baseline. After 12 weeks, a remarkable reduction was still found in the subjects with high level of mutans streptococci at baseline, but not different in the low and moderate level of mutans streptococci(p>0.05).

정신장애 환자의 심리사회적 문제사정 도구개발

김창곤,유수현,이혜원,채정호 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.4

Objeetives : There have been no consistent reliable and valid tool for examining the psychosocial problems for the patients with mental disorders in Korea. The purpose of the present study is to develop a scale that examine the psychosocial problems for the patients with mental disorders and to test its reliability and validity. Methods : To identify the psychosocial problems for 1,100 patients with mental disorders were selected in university hospitals and professional training institutes. And the items of the psychosocial problems were taken from them and divided them into individual and environmental categories. The representative items were established among these items of psychosocial problems and a questionnaire for a pilot study was made based on the representative items. The pilot study was done for 306 patients with mental disorders using the questionnaire to investigate reliability of the questionnaire. After the pilot study items and format of the questionnaire were revised and complemented. The revised questionnaire was given to 600 patients with mental disorders to test its validity and reliability and new scale for psychosocial problems was developed. Results : At the pilot study, which was performed with 1,100 patients, 3,200 items of psychosocial problems of 205 types were selected. Among these items 78 representative psychosocial problems items were withdrawn. Fifty-eight psychosocial problem items were finally set for the questionnaire after discussing them with the expert groups and examining its content reliability. Forty-seven psychosocial problems items were finally selected and given to 306 subjects at the pilot study. At the main study in which 600 subjects were tested inner-reliability. Correlation between items and scale was relevant and correlation coefficient between test and retest was high. The finally developed psychosocial problems testing scales were consisted of 46 testing items into 9 areas. Conclusion : The Psychosocial Problems Assessment Scale, which were developed at this study is proved to be valid and reliable and it will be able to be used to examine overall psychosocial problems for the patients with mental disorders in Korea.

Cariostat를 이용한 아동의 우식활성에 관한 임상적 연구

김재곤,이영수,양정숙,양연미,백병주 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.3

The purpose of the study was to determine the ability of the caries activity test, 'Car-iostat'to identify preschool children with dental caries, The subjects of this study were 76 children of 3 to 5 years of age. Oral examination was carried out to out to assess the caries experience using a mirror and explorer. In all subjects buccal plaque samples were obtained, incubated. and scored as the manufacturer's instructions for the cariostat test. statistical analyses were used with the Crosstabulation. The test group exhibited mean dft index 5.21, while 27.63% of subjects were caries free and had no restorations. The sensitivity of the Cariostat test was found to be 98.8% while the specifioity was found to be 19.05% The results showed that caries activity measured by visual reading was highly significant to the Cariostat score and dft index(P<0.001). the Cariostat method has indicated the future possible occurrence of caries. The use of this method is meant to require in the caries prevention program.

여러가지 수복물의 polishing조건에 따른 치수 온도변화

김재곤,이승영,박종하,백병주,양종숙 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.2

수복물의 연마의 중요성에 대하여 많은 연구들이 이루어져 왔으며, 이는 수복물의 금속성질의 개선, 변연적합성의 증가 그리고 치태축적의 감소 등을 이유로 시행된다. 그러나 불행하게도 수복물에 대한 연마시 열의 발생으로 인하여 치수부위에 손상을 초래하므로 열의 발생을 최소화하려는 노력이 뒤따라야 할 것이다. 이에 본 연구에서는 수복물의 연마시 회전속도의 차이, 냉각제의 적용 유무, 여러 종류의 수복물의 차이, 간헐적인 연마와 연속적인 연마의 차이 그리고 잔존 상아질의 두께의 정도에 따른 치수내부에서의 열의 변화를 알아보기 위하여 발거된 구치에 5급 와동을 형성한 후 복합레진(Z100, 3M co), resin-mod-ified GLC(Dyract, Fuji II LC), 그리고 아말감(Degussa)등으로 충전하였으며, 다양한 조건에서의 연마를 시행, 치수내부에서의 열의 변화를 측정하여 다음과 같은 결과를 얻었다. 1. Amalgam을 건조한 상태에서 연마시 다른 재료에 비해 큰 치수내 온도 상승을 보였다.(P<0.05). 그러나 Z100, Dyract, Fuji II LC간에는 간헐적 수복물 연마와 연속적인 수복물 연마 모두 어떤 경우든 유의차가 없었다.(P>0.05). 2. 같은 조건으로 연마를 시행했을 때, 간헐적인 연마보다 연속적인 연마를 한 경우가 치수내 온도가 더 크게 상승하였다.(p<0.01). 3. 아말감과 Dyract에 있어서는 잔존 상아질의 차이에 따른 치수내 온도변화에 유의한 차이를 보였으나(P<0.01), Fuji II LC에 있어서는 유의성이 없었다(P>0.01). 4. 냉각제의 유무에는 관계없이 어떤 경우든 시간에 따른 온도 상승을 보였다. 그러나, 냉각제를 사용한 경우가 냉각제를 사용하지 않은 경우에 비해 온도 상승값이 훨씬 작았다.(P<0.01) The importance of finishing and polishing the restoration has been described by several authors. The final step provides for improved metallurgical properties, better marginal adaptation, reduced plaque accumulation. Unfortunately, finishing of the restorations can produce damage from temperature rises at the pulpal wall. The aim of this study was to determine the changes in temperature can be occurred during the use of finishing and polishing instruments under a variety of conditions. ; with or without a water coolant, intermittent or continuous operation, high or low rotation speed, remaining dentin thickness, and various restorative materials. Class V preparations were cut on extracted molars and restored with composite resin(Z 100), resin-modified glass ionomer cements(Dyract, Fuji II LC), and amalgam. Finishing was done with aluminum oxide coated disc(Sof-lex polishing disc, 3M, USA). The following results were obtained. 1. The rise of temperature during polishing of amalgam restorations was the highest among the all experimental groups except polishing with water coolant(P<0.05). However, there were no statictical differences in temperature rises between Z 100, Dyract and Fuji II LC(P>0.05). 2. The intrapulpal temperature was greatly influenced by the applied time, and intermittent polishing was showed significantly lower temperature rises than continuous polishing(p<0.01). 3. The intrapulpal temperature was increased according to the application of polishing regardless of using water coolant. However, polishing with water coolant showed significantly lower temperature in the pulp than not used water coolant(P<0.01).

온도자극이 충전된 치질에 미치는 영향

김재곤,노용관,이영수,양정숙,백병주 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.2

본 연구에서는 수복에 따른 치질 내부 구조물의 열적변화를 평가하기 위하여 와동이 형성된 인공 치아모형에 아말감 충전, 아말감과 스테인레스 스틸관 수복, 금인레이와 금관수복, ZOE 이장 및 아말감 충전등으로 수복한 후 온도변화를 관찰하였다. 각 실험군에 4℃와 60℃로 2초와 4초 동안 자극을 가한 후 치아내부의 온도분포를 2차원적으로 분석하였고, 치수 중앙부에서 외측으로 층을 형성하여 3차원적인 해석을 시행한 결과, 다음과 같은 결론을 얻었다. 1. 4℃의 자극이 2초간 가해진 아말감 충전 경우, 아말감 충전과 스테인레스 스틸관을 장착한 경우에서는 1℃ 내외의 차이를 보였으며, 치수와 상아질 경계면에서 3초 경과시에 29℃까지 급격한 온도 하강을 보였으며 9초 후에 25℃에 도달하였다. 또한 금으로 수복한 경우에서는 3초 후 25℃까지 하강하여 그 온도를 유지하였으며, ZOE 이장 경우에서는 최저온도가 4℃ 이상 높게 나타났다. 2. 4℃ 자극을 4초간 가한 경우에서는 9초 후에서 2초 자극시보다 2-3℃ 낮았으며, 금 수복 경우에는 5초 후에 21℃의 최저온도를 보인 후 점차 회복되었다. 3. 아말감 충전 및 스테인레스 스틸관을 같이 장착한 경우에 60℃, 2초간 자극에는 3초 후 40℃의 온도로서 상승하였으나 9초 후에 30℃로 하강 하였으며, 금 수복에서는 2초 후 41℃까지 온도가 상승하였고, 9초 후 28℃를 보여 13℃의 차이가 있었다. ZOE 이장 경우에는 온도차이가 5℃로서 안정된 양상을 보였다. 4. 60℃, 4초 자극에 대한 아말감 충전 및 스테인레스 스틸관을 같이 장착한 경우에서 5초 후 42℃, 9초후 35℃를 보였으나 금 수복 경우에서는 3초 후 49℃, 9초 후 31℃의 온도변화를 나타내었다. 5. 3차원 분석에서 치수 중앙부에서 멀어질수록 온도변화가 심하였다. The dental structure substituted by restorative materials may produce discomfort resulting from hot or cold stimuli. To investigate the effects of this stimuli on the human teeth, thermal analysis was carried out by calculation of general heat conduction equation in a modeled tooth using numerical method. The method has been applied to axisymmetric and two-dimensional model, analyzing the effects of constant temperature 4℃ and 60℃. That thermal shock was provided for 2 seconds and 4 seconds, respectively and recovered to normal condition of 20℃ until 10 seconds. The thermal behavior of tooth covered with a crown of gold or stainless steel was compared with that of tooth without crown. At the same time, the effects of restorative materials(amalgam, gold and zinc oxide-eugenol(ZOE)) on the temperature of PDJ(pulpo-dentinal junction) has been studied. The geometry used for thermal analysis so far has been limited to two-dimensional as well as axisymmetric tooth models. But the general restorative tooth forms a cross shaped cavity which is no longer two-dimensional and axisymmetric. Therefore, in this study, the three-dimensional model was developed to investigate the effect of shape and size of cavity. This three-dimensional model might be used for further research to investigate the effects of restorative materials and cavity design on the thermal behavior of the real shaped tooth. The results were as follows: 1. When cold temperature of 4℃ was applied to the surface of the restored teeth with amalgam for 2 seconds and recovered to ambient temperature of 20℃, the PDJ temperature decreased rapidly to 29℃ until 3 seconds and reached to 25℃ after 9 seconds. This temperature decreased rather slowly with stainless steel crown, but kept similar temperature within 1℃ differences. Using the gold as a restorative material, the PDJ temperature decreased very fast due to the high thermal conductivity and reached near to 25℃ but the temperature after 9 seconds was similar to that in the teeth without crown. The effects of coldness could be attenuated with the ZOE situated under the cavity. The low thermal conductivity caused a delay in temperature decrease and keeps 4℃ higher than the temperature of other conditions after 9 seconds. 2. The elapse time of cold stimuli was increased also until 4 seconds and recovered to 20℃ after 4 seconds to 9 seconds. The temperature after 9 seconds was about 2-3℃ lower than the temperature of 2 seconds stimuli, but in case of gold restoration, the high thermal conductivity of gold caused the minimum temperature of 21℃ after 5 seconds and got warm to 23℃ after 9 seconds. 3. The effects of hot stimuli was also investigated with the temperature of 60℃. For 2 seconds stimuli, the temperature increased to 40℃ from the initial temperature of 35℃ after 3 seconds of stimuli and decreased to 30℃ after 9 seconds in the teeth without crown. This temperature was sensitive to surface temperature in the teeth with gold restoration. It increased rapidly to 41℃ from the initial temperature of 35℃ after 2 seconds and decreased to 28℃ after 9 seconds, which showed 13℃ temperature variations for 9 seconds upon the surface temperature. This temperature variations were only in the range of 5℃ by using ZOE in the bottom of cavity and showed maximum temperature of 37℃ after 3 seconds of stimuli. 4. In case of 4 seconds hot stimuli, the temperature increased 42℃ after 5 seconds and decreased 35℃ after 9 seconds in the teeth without crown. But in gold restoration, showed 49℃ after 3 seconds and 31℃ after 9 seconds, temperature variation was up to 18℃. Temperature variation of ZOE base case was only 5℃. 5. In three-dimensional analysis, we could find higher and lower thermal distribution pattern at the outer layer.

절제된 백서의 좌골 신경에서 절제 원위부의 변화에 따른 신경재생의 비교 실험연구

김정태,엄기일,김잉곤,류재만,이혜성 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

성숙 백서 좌골신경의 분지전 근위부에서 7㎜가량을 절제하여 신경 간격을 만든뒤 실라스틱도관 근위단에 절단된 신경의 근위부를 관통고정하고 원위부에는 아무것도 열결하지 않거나 좌골신경 절편, 종골건 절편, 안면신경 절편을 각각 관통고정시켜 제4주 및 8주에 신경 축색수를 대조군과 혹은 각 실험군끼리 비교하여 다음과 같은 결론을 얻었다. 1. 신경 절편을 연결헌 2,4군의 신경재생은 대조군과 거의 차이가 없었고(P>0.05) 아무것도 연결하지 않은 1군, 건절편을 연결한 3군에 비해 현격한 신경재생을 보였다.(P<0.01). 2. 신경 절편의공여부에 관계없이 2,4군의 실험군에서 각각 비슷한 신경재생을 보여 일단 신경이 손상되면 신경 성장인자가 손상된 신경단으로 부터 분비되며 종류에 관계없이 그 근위부의 신경 재생을 자극할 수 있음을 알 수 있었다. 3. 건절편을 연결한 3군의 실험군에서는 일부 신경재생이 일어나는 것으로 보아 여러가지 가능성을 제시할 수 있었다. 이상에서 같이 신경 재생에 대한 기전은 아직까지 밝혀지지 않고 많은 논란의 대상이 되고 있는 것이 사실이나 저자들은 이에 조금이라도 접근해보고자 본 실험을 시도하였고 많은 의문점이 제시되었으나 만족할 만한 결과를 얻었다. 앞으로 보다 더 세밀한 비교관찰과 많은 개체수를 이용한 실험이 필요하겠고 축색수 이외에도 수초의 두께, 수초와 축색의 비율, 유수 및 무수신경수의 비율등도 함께 비교하는 정확한 결과의 분석이 필요하리라 생각된다. An experimental model was presented for studying nerve regeneration over constant gaps of various distal stumps such as, empty tube(GroupⅠ), sciatic nerve segment(GroupⅡ), calcaneal tendon segment(Group Ⅲ), facial nerve segment(Group Ⅳ), to ascertain how the regenerating fibers of the proximal stump of the severed sciatic nerves of the rats are oriented and processed. In this experiment, the silastic tube was used as a guide to observe the sprouting of the regenerating fibers from proximal stump and then the regenerating fibers in the tubes were obtained and analyzed by light microcope, electron microscope and axon counts at 4 weeks and 8 weeks with the evoked action potential at 8 weeks. The results were as follows. 1. The axon counts of experimental group Ⅱ and Ⅳ with nerve segment stump were similar to those of their control groups(P>0.05) and were greater than those of experimental groupⅠ and Ⅲ(P<0.01). 2. The axon counts between the experimental group Ⅱ and Ⅳ were nearly indistinguishable and so nerve segments, irrespective of donor site in the same rat, induced the regenerating stimulus to their proximal stump of resected sciatic nerve. 3. In experimental group Ⅲ, the obtaining results of axon counts were not negligible, in spite of tendon stump, and the author could propose various possibilities. In conclusion, we could approach to the regenerating process of the nerve through the analysis of the above results but yet can not explain the exact mechanism. Anyway when the results are applied to the clinical situation, we would be helped much.