야생효모, Wickerhamomyces anomalus HO9-2로부터 급성 폐손상 억제물질인 호중구 elastase 저해물질 생산 및 생산균주의 균학적 특성

장지은, 문정수, 김하근, 이종수 배재대학교 자연과학연구소 2023 自然科學論文集 Vol.34 No.1

This study was performed to produce acute lung injury-preventing neutrophil elastase inhibitor from non-pathogenic wild yeast and further determine enzyme activity and physiological functionality of the potent wild yeast. Culture supernatants and cell-free extracts of one hundred twenty five non-pathogenic wild yeast strains were prepared and their neutrophil elastase inhibitory activities were measured. The neutrophil elastase inhibitory activity of the cell-free extract from Wickerhamomyces anomalus HO9-2 showed the highest inhibitory activity of 38.4% and its elastase inhibitor was maximally produced(IC50,3.2㎎) when Wickerhamomyces anomalus HO 9-2 was cultured in pH 7.0 of malt extract medium at 20℃ for 24h. Wickerhamomyces anomalus HO9-2 was oval shape and did not form ascospore and pseudomycelium. The HO9-2 strain grew well in YPD and YM media and was halophilic yeast which grew well in YPD medium containing 15% NaCl. Cell-free extract of the HO9-2 strain were showed high 17.2U of glucoamylase activity and anti-diabetic α-glucosidase inhibitory activity of 87.5%. 본 연구는 급성 폐손상 예방(또는 치료)의약 소재를 개발하기 위하여 비병원성 야생효모들의 elastase 저해활성을 측정하여 우수 균주를 선발하였다. 또한 선발균주의 elastase 저해제 생산 조건을 최적화 하였으며 선발균주의 미생물학적 특성 등을 조사하였다. 비병원성 야생효모인 Wickerhamomyces anomalus HO9-2의 무세포추출물이 38.4%의 호중구 elastase 저해활성을 보여 우수균주로 선발하였고 이 균을 pH 7.0 의 malt extract 배지에서 20℃로 24시간 배양하였을 때 최고의 elastase 저해활성(IC50, 3.2mg)을 보였다. 또한 이 균은 난형으로 포자와 의균사를 형성하지 않았고 YPD와 YM배지에서 생육이 양호하였고 NaCl을 15% 함유한 배지에서도 생육하는 호염성 효모이었다.

漁貝類의 低溫貯藏中의 品質變化에 對하여

張壽賢,金範淑,金種培 군산대학교 1976 群山水産專門大學 硏究報告 Vol.10 No.1

It is so far suggested from the results of investigation on the changes of quality during the freezing storage of shrimp and swell fish that increasing of VB-N, TMA rapidly break down, increasing of Coliform MPN and decreasing of concentration of pigment mainly in the early stage of storage. In this paper, the raw material used for experimental sample after soaking during 1hr. in 5% ascorbic acid in 2% NaCl solution and 5% EDTA solution. The results of this study was that the sea food of freezing storage for domestic use and expore had better soaking during 1hr. in 5% EDTA solution.

건축설계실무와 의사결정에 관한 사례 연구 : 공공프로젝트의 경우 Case of Buchon Welfare center and Yonsu Sub-municipal Office

장성준,서윤영,이현창,하종수 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-

The study analyzes a series of decisions made by Building Construction Review Committee on public building construction project. Several government authorities are engaged on the review and required the building client authority to revise the design according to revise items suggested by the committee members ranging from Architectural Planning to other Building Engineering fields. The study delves the legal and design aspects of the review, the meaning of private, semi-public and public function of building, and the construction policy. Two Public building projects were analyzed in terms of revise items. We found some patterns of problem and the current review system works to promote technical aspect of the building and to keep public entity of the projects.

시멘트 종류별 콘크리트의 적산온도에 의한 강도예측에 관한 연구

장종호,나철성,김재환,김용로,길배수,남재현,김무한 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

The purpose of this study is to exhibit fundamental data which can apply to quality and process management of structure concrete using special cement in the construction field investigating strength prediction by maturity method of concrete according to cement types. The activation energy value was derived from the experimental study that experimental factors were selected as W/C ratio of 45%, the kinds of cements of 3 levels of O.P.C, H.B.C, H.S.C and curing temperature of 3 levels of 5℃, 20℃, 35℃. Also, after investigating relationship between compression strength and equivalent ages of concrete according to cement types, the strength prediction was investigated by maturity method. As the above of results, differences between the prediction strength by strength prediction equation and the actual measurement strength were lower, therefore it is considered that the strength prediction by maturity method is possible not only concrete using normal portland cement but also concrete using special cement.

축구포지션별 무산소 운동능력 분석

손장식,정정욱,김종호,임정수,김훈 대구대학교 기초과학연구소 2003 基礎科學硏究 Vol.19 No.3

In this study, the Wingate power test was performed to investigate of the anaerobic power output in soccer players who play in different position. The main purpose of the test was to provide the enhancement of anaerobic capacity which was conducted by comparing and analysing of the Wingate test. This will improve the performance during the soccer games. Seventeen soccer players with three different positions, FW, MF, DF, were recruited, and undertook the Wingate power test, and anaerobic power, peak power output, anaerobic capacity, and fatigue index was analysed. There was a significant effect of a peak power output among positions showing MF>DF>FW (P<.05). MF revealed the highest peak power output at 15 and 30 seconds time points compared to DF and FW. As far as the players' positions were concerned, a relative anaerobic capacity showed a similar results with a peak power output (P<.05; MF>DF>FW). Unlike with these results, the fatigue index in different positions did not show any statistical significant results (P>.05). Based on these results, it was suggested that the systematical training program is required to perform a modern soccer, pressure and total soccer, in MF players. If the physical strength, especially power out, in MF players is enhanced, the results of the soccer games will be also improved.

한국어 판 구조화 임상면담도구 개발 : 신뢰도 연구

한오수,안준호,송선희,조맹제,김장규,배재남,조성진,정범수,서동우,함봉진,이동우,박종익,홍진표 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

연구목적: 정신장애의 정확한 진단과 평가는 임상에서뿐만 아니라 신뢰성 있는 연구를 위해서도 매우 중요하다. Structured Clinical Interview for DSM-IV(SCID)는 임상전문가들이 사용하는 진단도구로서, 비교적 짧은 시간에 정확한 진단을 내릴 수 있다. 본 연구는 한국어판SCID를 개발하고 그 신뢰도를 평가하기 위해 수행되었다. 방법: 국문학자가 포함된 번역위원회를 통하여 연구용판 SCID를 번역한 후, 정신과 의사 2인과 임상심리학자 1인에게 한국어판SCID 실시방법을 교육시킨 뒤 한국어판SCID를 이용한 면담의 평가자간 신뢰도 (interrater reliability)를 검증하였다. 면담 대상은 1999년 2월에서 3월까지 2개 병원 정신과에 치료중인 환자 90명(남:41명, 여:29명)이었다. 결과: 현재 장애(current disorder) 평가에서 주요 우울장애, 기분부전장애, 정신분열병, 알코올 남용 및 의존, 기타 물질 남용 또는 의존, 여러 불안장애들 및 섭식장애 등과 같은 대부분 장애의 kappa값은 .70이상으로 매우 높았다. 그 이외의 양극성 장애, 망상장애, 광장공포증, 감별 불능 신체화 장애 및 건강염려증의 kappa값도 .69에서 .40사이로 수용할 수 있는 정도였다. 평생 장애(lifetime disorder)에서는 양극성 장애(k=.69)와 감별 불능 신체화장애(k=.59)를 제외한 다른 모든 장애의 kappa값이 .70이상이었다. K-SCID 면담시 Ⅰ축 질환에 대하여 흔하게 다중 진단이 내려졌으며, 평균 진단 수는 1.5∼1.7개로 나타났다. 결론: 한국어판 SCID는 신뢰도가 높은 진단도구로 생각되며, 향후 정신질환의 정확한 진단과 임상연구에 유용하게 사용될 수 있다. Objectives: Accurate diagnosis and assessment for psychiatric disorders is crucial for research, as well as for clinical practice. Structured Clinical Interview for DSM-Ⅳ(SCID-RV) is a less time-consumimg and more accurate structured diagnostic interview form. It can be used by clinical professions and is known for a reliable diagnostic tool. Present study was conducted to develop Korean version of SCID-RV and to test the inter-rater reliability. Methods: The authors have translated original SCID-RV into Korean, and revised in parallel with sociocultural background of Korea. Ninety patients from two psychiatric hospitals, both outpatient and inpatient, were interviewed and rated independently by three raters. Results: The kappa coefficients for most of illnesses, such as major depressive disorder, dysthymia, schizophrenia, alcohol abuse and dependency, anxiety disorder and eating disorder were excellent(>0.70) in the evaluation of current disorders. And the kappa coefficients for bipolar disorder, delusional disorder, agoraphobia, undifferentiated somatoform disorder, and hypochondriasis were acceptable(>0.40) in the evaluation of current disorders. In the evalua-tion of lifetime disorders, the concordant rates of all the diagnoses except bipolar disorder and undifferentiated somatoform disorder were excellent. Lack of hierarchy in DSM-Ⅳ allows for multiple Axis I diagnoses. Mean numbers of Axis I diagnoses per subject assigned by the three raters were 1.5-1.7. Conclusions: Our findings confirm that SCID-RV yields highly reliable diagnoses. SCID-RV is recommended for accurate diagnosis in clinical practice and research on psychiatric disorders.

VDT작업별 정신사회적 스트레스와 근골격계 장애에 관한 연구

백남종,강종두,주영수,배인근,권호장,박종만,조수헌,김돈규,김재용,최홍렬 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

It has been hypothesized that jobs that have both high psychological demands and low decision latitude("job strain") can lead to musculoskeletal disorder. The objective of this study was to test whether job strain was correlated with the presence of work-related musculoskeletal disorder, especially myofascial pain syndrome(MPS). Information on demographic factors, confounders such as household load and taking care of children or not, and scores for decision latitude, job demand, and social support was obtained by self-administered questionnaire, which had been developed in Korean language, by adopting NIOSH instrument and Extended Karasek Model(16 items). All subjects were also examined by rehabilitation medicine specialists for musculoskeletal disorders. Subjects(n=370) could be categorized into 4 groups, these were, housewives(n=89), shipyard CAD workers(n=89), general female workers(n=79; nurses, insurance counselors, public officials, clerks, etc), and telephone directory assistance operators(n=113). Results from univariate analyses indicated that all demographic factors, all confounders, scores for decision latitude and social support were not associated with the risk of musculoskeletal disorder. However, score for job demand was higher in musculoskeletal disorder cases than others. In subgroup analysis, this association was convinced again, in telephone directory assistance operators. Job strain model showed that the group of telephone directory assistance operators was high-stain group, and OR of musculo skeletal disorder was 2.446(95% C. I. : 1.174, 5.096), when comparing this with the low strain group. In conclusion, job strain is a risk factor for work-related musculoskeletal disorder.

비병원성 야생효모 Rhodosporidium toruloides HGG35-2와 호중구 elastase 저해제 생성효모인 Wickerhamomyces anomalus HO9-2로부터 Killer toxin 생산

문정수, 장지은, 김하근, 이종수 배재대학교 자연과학연구소 2023 自然科學論文集 Vol.34 No.1

항진균 바이오의약의 소재인 killer toxin을 비병원성 야생효모로부터 생산하기 위하여 25개 비병원성 색소 생성 효모들의 killer toxin 활성을 측정하여 우수균주로 Rhodosporidium toruloides HGG35-2와 Wickerhamomyces anomalus HO9-2을 선발하였다. 이들 killer toxin 생성균주들의 효모 감수성 균주들에 대하여 killer 활성을 측정한 결과 시판 포도주등의 알콜발효로 이용되고 있는 Saccharomyces cerevisiae (La Parisienne®)에 대하여 우수한 항진균활성을 보였다. Killer toxin production of non-pathogenic wild yeasts, Rhodosporidium toruloides HGG35-2 and Wickerhamomyces anomalus HO9-2 were investigated. Rhodosporidium toruloides HGG35-2 showed strong killer activity against pathogenic yeast, Candida guilliermondii AHP28-1 and commercial alcohol fermentation yeast, Saccharomyces cerevisiae(La Parisienne). Wickerhamomyces anomalus HO9-2 also showed killer activity against Rhodotorula nothofagi JSC10-1 and commercial alcohol fermentation yeast, Saccharomyces cerevisiae(La Parisienne®).

지도자의 경쟁가치리더십과 선수만족 및 인지된 경기력의 관계

김종욱,양용길,이정수,장갑석,정종환 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

The purpose of this study was to identify the relationships among competing value leadership, athlete satisfaction and performance in sport field, The instrument for data collection was a questionnaire in which competing value leadership consisted as identified by Ha(1996), athlete satisfaction consisted as identified by Che(1997), and performance consisted as identified by An(2002), 350 subjects were selected from universities in Seoul by the stratified cluster sampling method, Among these selected subjects, 322 responded, and data analyses consisted of employing descriptive statistics, one-way ANOVA, multiple-regression using SPSS/PC version 11.0. First, competing value leadership and athlete satisfaction differ statistically and partially according to socio-demographic variables of athletes, Second, athlete satisfaction and performance statistically and significantly is influenced by competing value leadership of leader, Third, athlete satisfaction statistically and significantly influence performance of athletes.

합성수지 충전물을 이용한 충전탑에서 암모니아 처리에 관한 연구

김장호,김형호,김종수,김재홍,이경재 동아대학교 환경문제연구소 2003 硏究報告 Vol.25 No.2

In this study, packed column was chosen so as to remove ammonia gases, kinds of odor generated from industrial fields. Random packing 35mm Pallring pp., that is suitable for the environmental protection industry, was used because the packings are comparatively simple and easy to manufacture. Random packings 35mm Pallring, PP. has a form of lattice as plastic(po1ypropylene). By studying the characteristics for packing by experiment of dry pressure drop, hydraulic pressure drop, liquid hold-up and mass transfer, packed column is made as 300 [mm] in diameter, 1400 [mm] in height, the applicable criteria to the noxious gas treatment process was presented as follows.