腎皮質 S-Adenosyl-L-Methionine : Protein-Carboxyl O-Methyltransferase에 關한 硏究 Protein-Carboxyl O-Methyltransferase from Bovine Renal Cortex

宋晩秀,林圭,黃炳斗,李載欣 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

S-Adenosyl-L-methionine : protein-carboxyl 0-methyltransferase (EC 2.1. 1. 24, protein methylase II) was purified from bovine renal cortex approximately 6, 780-fold with a 9% yield by combination of ammonium sulfate precipitate, Sephadex G-75 chromatography, S-adenosyl-L-homocysteine-sepharose 4B chromatography and hydroxyapatite chromatography, and then its properties and physiological role were investigated. 1. The enzyme showed a pH optimum around 5.6-6. 0. It is heat labile, inactivated by heattreatment for 3 minutes at 60℃, and when stored at -20℃ in the presence of 10% glycerol, its activity has been almost stable in a month. 2. Copper ion (Cu^2+}) and zinc ion (Zn^2+) were a potent inhibitors, the activity being completely inhibited at 2 mM. The inhibition of copper and zinc ions were recovered 85%and 63% of its activity adding of 4 mM EDTA, respectively. The inhibition of copper ion was completely recovered by adding of 10 mM mercaptoethanol, but zinc ion did not almost affect. 3. The apparent Km value for S-adenosyl-L-methionine was 4 x 10^-6M. Kinetic analysis of the enzyme in the presence of 25 uM copper ion showed that the nature of the inhibition to the enzyme was noncompetitive, considering that Km was constant and Vmax was decreased, and 50uM zinc ion showed that it was uncompetitive, considering that both Km and Vmax were decreased. 4. Immunoglobulin A, histone II and histone H3 were relatively good substrates for the enzyme. 5. The enzyme activity was completely inhibited by 0.4 mM of p-hydroxymercuribenzoic acid, but all of the activity was recovered in adding 10 mM mercaptoethanol, and the molecular weight of the enzyme was 24, 000. 6. The carboxylmethylated fractions of the brush-border membrane of bovine renal cortex had molecular weight of 70,000, 67,000, 45,000, 40,000, 31,000 and 27,000 etc. (6 fractions), as determined by SDS/polyacrylamide gel electrophoresis. 7. The carboxylmethylation of the brush-border membrane increased with time, S-adenosylmethionine, substrate concentration, and methylated brush-border membrane was demethylated by 50% for 10 minutes at pH 7.4. 8. D-Glucose uptake was inhibited by carboxylmethylation of brush-border membrane. These results suggest that protein methylase II may play some roles in regulating physiological function of brush-border membrane.

Pseudomonas aeruginosa와 Acinetobacter baumannii에 대한 Imipenem, Panipenem 및 Meropenem의 시험관내 항균력 비교

송원근,우흥정,강희정,김재석,박민정,이규만 대한화학요법학회 2002 대한화학요법학회지 Vol.20 No.3

목적 : Pseudomonas aeruginosa와 Acinetobacter baumannii는 중요한 병원감염균으로 알려져 있으며 다약제 내성을 잘 유발한다. 최근 들어 전 세계적으로 carbapenem제에 대한 감수성이 떨어지고 있어 문제가 되고 있다. 이에 저자들은 한국에서 분리된 P. aeruginosa와 A. baumannii를 대상으로 imipenem, panipenem 및 meropenem의 항균력을 비교하고자 하였다. 방법 : 150주의 P.aeruginosa (imipenem 감수성, 62주; imipenem 내성. 88주)와 53주의 A.baumannii (imipenem 감수성, 9주; imipenem 내성 44주)를 대상으로 National Committee for Clinical Laboratory Standards의 기준에 따라 미량액체배지희석법으로 imipenem, panipenem. meropenem에 대한 최소억제농도(MIC)를 측정하였다. 결과 : 총 150주의 P. aeruginsa 균주의 MIC_50/MIC_90은 imipenem, panipenem 및 meropenem이 각각 16/64, 16/64 및 4/32㎍/mL이었다, 53주의 A.baumannii 균중의 MIC_50/MIC_90은 imipenem, panipenem 및 meropenem이 각각 32/64, 32/64 및 16/32 ㎍/mL이었다. 88주의 imipenem 내성 P. aeruginosa 균주 중 33주 (38%)는 감수성 결과를 보였으나 그 반대의 결과를 보인 경우는 없었다. 결과 : MIC 결과 상 imipenem에 내성인 P. aeruginosa와 A. baumannii에 대하여 meropenem이 impenem이나 panipenem보다 좀더 강한 활성이 있음을 알 수 있었다. Background : Pseidomonas aeruginosa and Acinetobacter baumannii are important nosocomial pathogens. Many strains are multi-drug resistant, with decreased susceptibility to carbapenem emerging worldwide. We investigated the in vitro activity of imipenem, panipenem, and meropenem against P. aeruginosa and A. baumannii isolates in Korea. Methods : One hundred and fifty strains of P. aeruginosa (imipenem-susceptible, 62strains; imipenem-resistant, 88 strains) and 53 strains of A baumannii (imipenem-susceptible, 9 strains; imipenem-resistant, 44 strains) were examined. Minimal inhibitory concentration (MIC) testing for imipenem, panipenem, and meropenem was performed using the broth microdilution method as described by the National Committee for Clinical Laboratory Standards. Resilts : The MIC_50/MIC_90 of imipenem, panipenem, and meropenem against the clinical isolates were: 16/64, 16/64, and 4/32 ㎍/mL, respectively, against P. aeruginosa; 32/64, 32/64, and 16/32 ㎍/mL against A. baumannii. 33 (38%) of the 88 strains of impenem-resistant P. aeruginosa were susceptible to meropenem but the reverse was observed in none. Conclusion : The MIC data showed that meropenem was more active than imipenem and panipenem against imipenem-resistant P. aeruginosa and A. baumannii isolates.

낭성법랑모세포종, 함치성낭, 치성각화낭의 방사선소견과 Ki-67, PCNA, Cytokeratin 발현과의 연관성에 관한 연구

송만용,이삼선,이진구,이원진,허민석,이재일,민병무,최순철 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To compare the proliferation potential of the epithelial cells between unicystic ameloblastoma (UA), dentigerous cyst (DC), and odontogenic keratocyst (OKC) and to correlate this proliferation potential with the radiographic features of these three pathoses. Materials and Methods : Immunohistochemical expression of PCNA, Ki-67, and cytokeratin as a proliferation marker were assessed for 15 cases of UA, 15 cases of DC, and 15 cases of OKC. The degree of immunochemical expression of three proliferation markers were correlated with the radiographic features, especially cortical expansion (negative and positive) and shape of border (scalloped and round). Results : Using PCNA and Ki-67, OKC showed the highest proliferation potential and UA the lowest. Statistically significant differences were found between the OKC and the UA (p.0.05). However, no statistically significant difference was present according to the radiographic features in all pathoses. Using cytokeratin, there was no significant differences of proliferation potential among three pathoses. Conclusions : OKC epithelium has the most intense proliferation potential, followed by the dentigeous cyst and then unicystic ameloblastoma. There is no significant relation between the radiographic features and the proliferation potential of epithelium of these three pathoses.

컴플라이언스를 실현할 수 있는 매니퓰레이터 선단부의 기계적 구조의 설계

송재성,서만승 동명정보대학교 1999 東明情報大學校論文集 Vol.2 No.-

An analytical method for the design of compliant end-effector appropriate to the given assembly task is presented. Use of passive compliance in mating operations is effective in order to prevent excessive contact forces and to guide a part to be assembled to its goal location despite of positioning errors. First, quasi-static equilibrium conditions are established based on the kinematic and quasi-static analyses of mechanical contacts. Second, feasible candidates for the desired end-point compliance are calculated based on the equilibrium conditions. Next, the configurations and the spring constants of virtual springs consisting of the desired compliant mechanism are designed. Simulation of mating operations is then introduced in order to determine the spring constants and to verify whether the derived compliant mechanism enables the operations successful or not. Finally, one numerical example is shown to illustrate the proposed design method.

고객주문에 기초한 자율분산형 생산시스템에 관한 연구

송재성,서만승 동명정보대학교 2000 東明情報大學校論文集 Vol.3 No.-

We present a new framework for an autonomous distributed manufacturing system to improve the system efficiency as well as to cope flexibly with environmental change. When a customer order inputted, some alternatives for working group and their production schedules are made through autonomous decision making of the system constituents, and an optimal resources group is allocated through the bidding process based on a contract net protocol. In the proposed system, the concept of transition lots are introduced in order to cope with one to middle volume production and to improve the efficiency. Especially, for the purpose of the latter, an intermediate conceptual agent called process agent is created by a constituent who is willing with leadership to fulfill the bid order, organizes a working group, and manages the task execution, whereas these roles are played by a central computer in traditional distributed manufacturing systems. Every constituent makes decisions and actions so as to maximize its possessing value, and the overall behavior of the system is controlled by the value distribution.

국내의 Acinetobacter baumannii와 Pseudomonas aeruginosa 임상분리주에 대한 Colistin과 Polymyxin B의 감수성 양상

송원근,이태재,박민정,김한성,김재석,우흥정,이규만 대한감염학회 2006 감염과 화학요법 Vol.38 No.6

배경 : 다약제 내성 Acinetobacter baumannii와 Pseudomonas aeruginosa에 의한 감염의 증가로 인해 기존의 치료제를 대신할 수 있는 colistin이나 polymyxin B와 같은 대체 항균제들이 사용되고 있다. 이에 국내에서 분리된 A. baumannii와 P. aeruginosa에 대한 colistin과 polymyxin B의 감수성 현황을 알아보고자 하였다. 재료 및 방법 : 국내 5개 대학병원에서 분리된 균주 중 ceftazidime과 imipenem의 감수성에 따라 93주의 A. baumannii와 99주의 P. aeruginosa를 선택하였다. 액체배지 미량희석법으로 colistin과 polymyxin B의 감수성을 시험하였다. 결과 : Ceftazidime과 imipenem이 감수성인 균주에서는 기타 β-lactam제, aminoglycoside제 및 ciprofloxacin에 대한 감수성율(각각 58-100%, 50-100%, 75-100%)이 높았으나, ceftazidime 또는 imipenem이 내성인 균주에서는 위의 항균제에 대한 감수성율이(각각 ≤31%, ≤47%, ≤18%)이 낮았다(P<0.05). Colistin과 polymyxin B에 대한 A baumannii (MIC_(50/90), 1/2 ㎍/mL)와 P. aeruginosa(MIC_(50/90), 1/1 ㎍/mL)에의 항균 범위는 거의 유사하였고 두 약제 모두 매우 우수한 항균력을 보였다. A. baumannii에서는 1주(1.1%)가 colistin 내성(MIC, 4 ㎍/ mL)이 었으나 polymyxin B에는 감수성(MIC, 2 ㎍/mL)이었으며 이외의 모든 A. baumannii와 P. aeruginosa는 colistin과 polymyxin B에 감수성(MIC, ≤2 ㎍/mL)이었다. 결론 : 국내에서 colistin과 polymyxin B에 둘 다 내성인 A. baumannii나 P. aeruginosa 감염은 없었다. 따라서polymyxin제를 다약제 내성 A. baumannii나 P. aeruginosa 감염에 대한 대체 항균제로 유용하게 사용할 수 있을 것으로 생각된다. Background : The emergence of multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa has become a major problem. The use of nontraditional agents such as colistin and polymyxin B have been tried. The purpose of this study was to evaluate the colistin and polymyxin B susceptibility of A. baumannii and P. aeruginosa isolates in Korea. Materials and Methods : According to susceptibility of ceftazidime and imipenem, selected 93 isolates of A. baumannii and 99 isolates of P. aeruginosa were collected from 5 university hospitals in Korea. Susceptibility to colistin and polymyxin B was tested using the reference broth microdilution method. Results : The rates of other β-lactams, aminoglycosides, and ciprofloxacin susceptibility were high (58-100%, 50-100%, and 75-100%, respectively) in ceftazidime- and imipenem-susceptible isolates but were low (≤31%, ≤47%, and ≤18%, respectively) in ceftazidime- or imipenem-resistant isolates (P<0.05). Colistin and polymyxin B displayed a nearly identical spectrum of activity, exhibiting excellent potency against A. baumannii (MIC_(50/90), 1/2 ㎍/mL) and P. aeruginosa (MIC_(50/90), 1/1 ㎍/mL). Only one of the A. baumannii isolates was resistant to colistin (MIC, 4 ㎍/mL), but the isolate was susceptible to polymyxin B (MIC, 2 ㎍/mL). Conclusion : In Korea, no A. baumannii and P. aeruginosa isolates were resistant to both colistin and polymyxin B. These data suggested that polymyxins may be alternative drugs for multidrug- resistant A. baumannii and P. aeruginosa isolates.

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

분자역학적 방법을 이용한 부산시내 대형건물 냉각탑수의 레지오넬라균의 형별 분류

김정만,정석훈,서대영,박은희,송은주,최재철,이은엽,장철훈 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

섬이 있는 포켓의 가공을 위한 최적공구경로의 결정에 관한 연구

서만승,송재성,김혜령 동명정보대학교 2000 東明情報大學校論文集 Vol.3 No.-

In area milling such as pocket machining with islands, optimal tool path planning is very difficult problem. In this paper, new algorithm is presented, which determine it. Our algorithm is consisting of three modules; (1)generating the offset curves, (2)constructing and removing the offset loop and between loops, (3)finding the shortest path for minimal tool movement, and the uncut areas. For optimal tool path planning, we make use of the concepts of loop entity, which is chain of offset curves of contour curves. It is an entity that treat systematically both the intersection of offset curves and detection of an unuseful offset curves. And our algorithm is also applied to path planning for uncut area without the modification of our algorithm. Emprical tests show that the proposed algorithm fulfils its requirements.

국내기업 적용을 위한 학습민첩성의 개념정의와 구성요인에 관한 탐색적 연구

강금만(Kang, Kum-man),김민재(Kim, Min-jae),송영수(Song, Youngsoo) 한국경영교육학회 2020 경영교육연구 Vol.35 No.6

[연구목적] 학습민첩성은 Lombardo and Eichinger(2000)가 그 개념을 최초로 주창한 이래 다양한 관점에서 개념정의와 구성요인 연구가 이루어졌으나 국내기업에의 적용을 위한 목적에서의 연구는 상대적으로 미흡하였다. 이에 실천적 관점에서 국내기업에의 적용을 위한 학습민첩성의 개념정의와 구성요인을 탐색해보고 시사점을 제공하고자 하였다. [연구방법] 본 연구는 선행연구에 대한 문헌고찰, 국내 인적자원개발 관련 산학연 전문가를 활용한 델파이 기법, 초점집단면접을 연구방법으로 채택함으로써 삼각검증을 실시하였다. 먼저 문헌고찰을 통해 사전적 구성요인을 도출하였고, 산학연 전문가 23명으로부터 3차에 걸친 델파이조사를 통하여 개념정의와 구성요인을 확인하였고, 2차와 3차 델파이조사 뒤에 초점집단면접을 실시하여 최종적인 개념정의와 구성요인을 확정하였다. 이 과정에서 내용타당도비율(CVR)을 파악하여 델파이조사의 타당도를 검토하였다. [연구결과] 국내기업에의 실질적 적용을 위한 학습민첩성의 개념적 정의에 있어서 포괄적 접근 관점에서의 개념정의가 이루어졌다. 구성요인은 크게 성장지향, 자아성찰, 관계지향, 변화지향, 유연한 사고, 도전적 실험의 6개가 도출되었다. [연구의 시사점] 학습민첩성 구성요인을 확인함으로써 실천적 관점에서의 교육과정 개발과 상관관계 분석을 위한 토대를 제공할 수 있다. [Purpose] Since Lombardo and Eichinger(2000) advocated the concept of learning agility, a lot of studies had been conducted worldwide. But the research for the purpose of applying to Korean companies is relatively insufficient. The purpose of this study is to explore the conceptual definition and constructs of learning agility from a practical point of view. [Methodology] For this purpose, we conducted triangulation by adopting literature review, 3-round Delphi method, and 2-round focus group interview. 23 experts from universities, research institutes and industries participated in the Delphi survey. CVR(Content Validity Ratio) was used as a criteria for the selection of constructs. [Findings] The characteristic of learning agility concept derived from Delphi method and FGI is that it takes the comprehensive approach. 6 major constructs of growth-oriented, self-reflective, relationship-oriented, change-oriented, flexible thinking, and challenging experiments are derived. [Implications] Identified constructs provide a foundation for curriculum development and correlation analysis from a practical perspective. As a result, it can help reinforce learning agility in Korean companies.