Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

Lidocaine의 산성도가 경막외 마취에 미치는 영향

이원형,신효철,신용섭 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

The affective factors on regional anesthesia are lipophilic-hydrophilic balance of local anesthetics, protein binding, hydrogen concentration, structure of intermediate chain, and etc. Local anesthetics in solution exit in a chemical equilibrium between the basic uncharged form(B) and the charged cationic form(BH^+) by Henderson-Hasselbalch equation(PH_a=PK+log[B]/(BH^+)). The increasement of uncharged form brig about rapid onset, good quality, and long duration of anesthesia. Eight patients in group Ⅰ were given 2% lidocaine 21.7ml with 1: 200,000 epinephrine(pH 5.1), and eight patients in group Ⅱ were given 2% lidocaine 20ml with 1: 200,000 epinephrine added NaHCO_3 1.7ml(pH 7.2) to determine the epidural analgesic effect of the pH-adjusted solution. The results are summerized as follow 1. The onsets of analgesia and surgical anesthesia were significantly rapid in group Ⅱ. 2. The levels of analgesia were raised more rapidly in group.

아라키돈산 및 아이코사펜타엔산 생성 곰팡이의 분리 및 생산

신효선,오인호,이장한 동국대학교 산업기술환경대학원 1994 산업기술논총 Vol.2 No.-

Fungal strains producing extraordinary high amounts of arachidonic acid(ARA) and eicosapentaenoic acid(EPA) were isolated from soil samples and the effects of various culture condition on the production of ARA and EPA were investigated. Three strains of fungi with the high acids yields were identified as Mortierella alpina 49, M. parvispora 58 and M. horticola 73. Highest amounts of EPA and ARA were produced when three strains were cultivated at 18℃ for 4 and 6 days, respectively. When the three strains of fungi were grown in a medium with increase of 5% glucose, EPA production was decreased in every case, whereas ARA production not decreased in every case. There was no significant effect of oxygen on the fungi growth. But the production of EPA and ARA as well as the total fatty acids decreased when cultivated in 200 ml of culture volume in 500 ml flask. When the fungi cultivated for 6 days at 18℃ and stand for 6 days at room temperature, there was no difference in growth amount, while there was increase in the total fatty acid production, i.e., 36% increase as for M. parvispora 58 and 10∼20% as for the other two fungi. Aging didn't influence the EPA production much. the amount of the fungi growth and the total fatty acid production increased with more soybean oil addition as a nutrient of culture medium.

Sol-Gel Process를 利用한 Y-SrTiO_3 微分體 合成

申孝順,이병교 경북대학교 산업기술연구소 1992 産業技術硏究誌 Vol.20 No.-

Y-SrTiO_3 powders are synthesized by pechini process as a kind of sol-gel process and compared with powders by solid state reaction with respect to powder properties. As a result, calcination temperature is lowered about at 500℃. Furthermore, obtained powders are fine and homogeneous. And these powders are agglomerated and the size of primary particles is about 30㎚.

노부모의 성인자녀와의 갈등 및 우울에 관한 연구

이윤정,이신숙,신효식 全南大學校家政科學硏究所 1994 生活科學硏究 Vol.4 No.-

This study was to investigate the depression of the aged parents perceived through the conflicts between the aged parents and their adult children. The subjects of the study were 469 old parents, consisted of 234 male and 235 female over 60 years old lives in Kwangju. The datas were analysed by frequency, percentage, mean, standard deviation, oneway ANOVA, t test, Duncan's test and multiple regression using SAS program and the results are as follows. 1) In the conflict between the aged parents and their adult children which the aged parents perceive, the mean values are 32.54 in the case of old father and 34.26 in old mother from the average point. The average values of the depression the aged parents perceive are 26.03 and 28.35 each in old father and old mother and show the level of depression over the middle value. 2) The background variables which show the significant differences of the conflict between the aged parents and their adult children are marital status, age, education, pocket money, health and activity of the aged parents. 3) The background variables which show the significant difference in the depression the aged parents perceive are marital atatus, education, pocket money, health, activity, support type and occupation of the aged parents. 4) The depression and conflict between the aged parents and their adult children show high correlations and the greatest variable as the effect of conflict on the depression is emotional conflict. In addition, significant difference is shown depending on health, value and economic conflict and its explanatory power is 50%.

고등학생의 성폭력 경험과 인식

신효식,이경주 한국 가정과 교육 학회 2003 한국가정과교육학회지 Vol.15 No.1

The purpose of this study is to investigate the cognition and experience of sexual violence and to present the influential factors of the cognition of sexual violence. The subjects were 835 high school students living in Kwangju and Chonnam. The data analysis used Mean, Standard Deviation, T-test, F-test, and Multiple Regression Analysis. The major findings is as follows; 1. There are meaningful differences in the experience of sexual violence between male and female adolescents. 2. There are meaningful differences in the cognition of sexual violence in sex, sex-role attitude, experience of obscene materials and sex education. 3. The variables which affect cognition of sexual violence is sex-role attitude, sex and experience of obscene materials that were explained about 10%.

Quick Response 기반의 Mass-Customization구현을 위한 점포유형에 따른 직물감성에 관한 소비자 태도 연구

신상무,이효정 한국의류학회 2002 한국의류학회지 Vol.26 No.11

Quick Response based Mass-Customization can be produced and distributed customized goods and services on mass basis in apparel e-business. Because consumers can't touch and feel the apparel products in e-business, they tend to have the negative buying behavior. The purpose of this study is to investigate the differences of consumer's texture sensibility of apparel products based on marketing channels(on-line/off-line). Two types of questionnaires for on-line and off-line were used to assess consumer sensibility on apparel fabric. The 8 swatches were selected in regard to the previous literatures. 205 questionnaires for each type (on-line/off-line) were distributed. Statistical devices were t-test, mean and standard deviation with SPSS_(10.0.) The result of this study was showed that there were partially significant differences on consumers' texture sensibility on apparel products between on-line and off-line. Under on-line environment, consumers perceived corduroy as warm, strong, and sandy. taffeta as warm, sandy, and glossy, denim as sandy, and warm, organza as sandy, and thin, satin as sandy, dense, and modern, chiffon as sandy, and fiat, velvet as warm, and soft, single jersey as warm, soft, and comfortable. Therefore, apparel firms cooperating QR based Mass-Customization in e-business have to pay attention to the differences on consumers' texture sensibility of on-line apparel products from those of off-line.

심상성 루프스에서 Nested-Primer Gene Amplification Assay에 의한 결핵균 DNA의 검출

신문석,전효진,김병천,이규석,김재룡,권호준,송준영 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

Lupus vulgaris, which is a progressive form of postprimary tuberculosis in patients with a moderate to high degree of immunity and tuberculin sensitivity, is the most common, most serious, and most variable type of cutaneous and mucous membrane tuberculosis. Domonstration of M.tuberculosis directly or in culture in some of these eruptions can be difficult. It is essential to demonstrate the presence of M.tuberculosis in a clinical sample for a definitive diagnosis of tuberculosis. With the discovery of the polymerase chain reaction (PCR), the DNA diagnostic approach for infection has developed in a short period. With the dual purpose of definitive and rapid diagnosis of tuberculosis, this study examined the usefulness of a nested PCR for detection of M. turberculosis. Compared with a single-step PCR, the two-step PCR was able to enhance sensitivity approximately 1,000-fold on the basis of bacterial counts and DNA quantity. We concluded that two-step nested PCR appeared to be the most useful PCR protocol for early and sensitive diagnostic method detecting M.tuberculosis in acid-fast stain negative specimens.

집적화된 실리콘 압력센서의 출력전압 보상파라미터 추출 및 그 특성

이보나,김건년,박효덕,신상모,이경탁,김찬,권혁채,이상조,박현주 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

An integrated silicon pressure sensor has been designed, fabricated and tested. The signal conditioning circuits were designed to include calibration and temperature compensation of output voltage through trimming of diffusion and ion-implanted resistors. Before trimming of resistors, the compensation parameters such as pressure sensitivity, temperature coefficient of pressure sensitivity, temperature coefficient of piezoresistors and pressure sensitivity of piezoresistors were measured. Then offset voltage, span, and temperature coefficients of offset voltage and span were calibrated by trimming of resistors. The measured output voltage met our design specification and simulation value above room temperature. But, the measured output voltage at -30°C deviated from our design specification and simulation value because the offset voltages were found to vary randomly as a function of temperature.

들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.